The preparation and the quality control of cross-linked agarose coated activated (CAAC-II) were described. The crosslinking r eagent epichlorohydrin was removed. Investigations indicate that the trace elements such as Pb, Cd, As, Cr, Fe and heavy metals, the pH, NH4+, SO42- , and ultraviolet a bsorbance in sample have met the requirements in Chinese Pharmacopeia and some other relevant standard promulgated by the Ministry of Health. No epichloro hydrin was detected in CAAC-II.

The preparation and properties of a new adsorbent cross-linked agar beads entrapped attapulgite clay (CAA) are reported in detail. Medicinal attapulgite clay made in China was encapsulated with agar, shaped in organic solvent, and cross-linked by epichlorohydrin . The products withstood autoclaving at 121 ℃ for half an hour with no breakage, and had a good selective adsorption ability for some model compounds and medicaments such as methylene blue and phenothiazine drugs. Preliminary investigations and SEM photographs indicate that the CAA as an adsorbent is relatively hemocompatible for hemoperfusion.

The leukocytic phagocytosis rate and the index of phagocytosis of rats on cross-linked agar beads entrapped attapulgite clay (CAA) hemoperfusion were studied. The results revealed that the leukocytic phagocytosis rate and the index of phagocytosis descended significantly after 1 hour and rose gradually after 6 hours. Finally it reached the normal level after 48 hours. Hemoperfusion repeated two times gave similar results. In conclusion, the function of leukocytic phagocytosis declined temporarily during CAA hemoperfusion. Many times hemoperfusion will not notably affect the body's defense system of rats.
Agar ; Animals ; Biocompatible Materials ; Hemoperfusion ; methods ; Leukocytes ; drug effects ; physiology ; Magnesium Compounds ; Male ; Materials Testing ; Microspheres ; Phagocytosis ; drug effects ; Rats ; Rats, Wistar ; Silicon Compounds

The adsorption of methylene blue (MB) in plasma on cross-linked agarose beads entrapped activated charcoal (CAAC-I), cross-linked agarose coated activated charcoal (CAAC-II) and cross-linked agar beads entrapped attapulgite (CAA) was tested in this study. These adsorbents are intended to be applied to blood purification for eliminating MB from virucidal MB-phototrated plasma units. The experimental results indicated that the adsorption of MB in plasma on CAAC-I and CAA was quick and efficacious, but it was slower on CAAC-II. The relationship between the adsorption time and the adsorption rate was examined with reference to the plasma with a specific concentration of MB flowing over a certain volume of CAA. A flow rate of about 1 ml.min-1 through 10 ml CAA at 25 degrees C could eliminate more than 95% of the MB in 200 ml human plasma (1 mumol.L-1).
Adsorption ; Agar ; chemistry ; Animals ; Charcoal ; chemistry ; Hemofiltration ; instrumentation ; Humans ; In Vitro Techniques ; Methylene Blue ; isolation & purification ; Rabbits