Five meroterpenoids, rhodonoids K-M (1-2), daurichromene E (3), and grifolins A-B (4-5), together with seven known compounds (6-12), were isolated from Rhododendron anthopogonoides. The chemical structures of these compounds were elucidated through comprehensive analysis of high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), ultraviolet (UV), infrared spectroscopy (IR), and nuclear magnetic resonance (NMR) data. Their absolute configurations were determined by comparing experimental electronic circular dichroism (ECD) spectra with computed values. Notably, compounds 1 and 3 demonstrated significant inhibitory effects on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells. These compounds markedly suppressed the mRNA expressions of inflammatory factors, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) while also down-regulating the protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2).
Mice ; Rhododendron/chemistry* ; Animals ; Anti-Inflammatory Agents/isolation & purification* ; RAW 264.7 Cells ; Terpenes/isolation & purification* ; Molecular Structure ; Tumor Necrosis Factor-alpha/immunology* ; Cyclooxygenase 2/immunology* ; Nitric Oxide Synthase Type II/immunology* ; Macrophages/immunology* ; Interleukin-6/immunology* ; Lipopolysaccharides ; Interleukin-1beta/immunology*

With the progress of information technology and intelligent technology,the intelligent development of urine testing instruments is facing new opportunities.Using the disease cybernetics theory model to analyze the business process and current urine testing instruments of clinical urine analyzer,a generational theoretical model of urine testing instruments has been constructed,which is conducive to guiding the intelligent development direction of urine testing instruments.The study divides urine testing instruments into one to four generations of products,with the first-generation of products being operated by doctors.The second-generation products are currently available for laboratory technicians to use various urine analyzers.The third-generation products further optimize the testing process and intelligence,without the need for inspectors to operate.The fourth-generation products are unmanned and do not require sampling.It can be seen that with the development of technology,urine analysis has indeed become more convenient,but after all,various instruments have their limitations.Therefore,the establishment of a theoretical model for the generation of urine testing instruments should be applied in clinical urine testing,which can not only improve the efficiency of urine analysis but also improve its quality.

Objective:To evaluate and compare the early and long-term outcomes of rheumatic patients who underwent left-side heart valve replacement with concomitant tricuspid valve replacement, and analyze the difference of early mortality and long-term survival rate between mild pulmonary artery systolic pressure (PASP) and moderate to severe PASP patients.Methods:From January 2009 to December 2018, 79 cases of patients were reviewed and summarized. The mean age before operation was (45.7±7.8) years old. These patients were divided into mild PASP group(<50 mmHg, 38 cases) (1 mmHg=0.133 kPa) and moderate-severe PASP group (>50 mmHg, 41 cases) . Kaplan- Meier method was used to estimate the overall long-term survival rate and the incidence of complications, and to compare the long-term survival rate of patients with mild and moderate -severe PASP elevation. Results:The mortality rate of early postoperative patients was 8.9% (7/79). The causes of death included: low cardiac output syndrome in 3 cases, multiple organ failure caused by pulmonary infection in 2 cases, acute renal insufficiency in 1 case, sudden cardiac arrest in 1 case. Although the mortality rate of early moderate-severe PASP group (12.2% vs. 5.3%) was higher than that of mild PASP group, there was no significant difference ( P>0.05). The mean follow-up time was (51.8±31.7) months (3-115 months). Kaplan- Meier method was used to estimate the 10-year survival rate, the rate of avoiding thrombosis, the rate of avoiding serious bleeding event and the rate of avoiding reoperation, which were (67.2±10.6)%, (85.7±6.2)%, (83.4± 6.9)% and (93.7 ± 3.7)%, respectively. Although the long-term survival rate of mild PASP (78.0±10.6) % was higher than that of moderate-severe PASP (62.8 ± 13.4) %, there was no significant difference ( P>0.05). Conclusion:The early mortality rate of rheumatic patients who underwent left-side heart valve replacement with concomitant tricuspid valve replacement is still relatively high. The recurrent heart failure is the major causes of long-term death.

Objective To evaluate the effectiveness and safety of modified Zuogui Pill combined with hormone in the treatment of premature ovarian failure ( POF) . Methods Randomized controlled trials were carried out by searching Chinese and English databases from the establishing to June, 2018. The meth-odological quality was assessed in the included randomized controlled trials. The treatment of premature o-varian failure by modified Zuogui Pill combined with hormone was systematically evaluated by RevMan 5. 3 software. Results 11 randomized controlled trials involving 1004 patients were included. Meta-analysis results showed that total effective rate (OR=4. 44, 95％ CI: 3. 10 -6. 36, P<0. 00001), Tranditional Chinese Medcine ( TCM ) syndromes ( including dizziness, tinnitus, hot flash night sweats, five upset fe-ver, waist and knees), serum follicle stimulating hormone ( FSH) level [ standardised mean difference (SMD) =0. 54, 95％ CI:0. 13-0. 95, P=0. 01], luteinizing hormone (LH) level (SMD=0. 90, 95％CI:0. 45-1. 36, P<0. 0001), estradiol (E2)level (SMD=1. 26, 95％ CI:0. 12-2. 40, P=0. 03), endometrial thickness [weighted mean difference (WMD) =2. 03, 95％ CI:0. 07-3. 99, P=0. 04] im-provement and the incidence of adverse reactions (OR=0. 34, 95％ CI:0. 12-0. 95, P=0. 04) in modi-fied Zuogui Pill combined with hormone therapy were superior to those of pure hormone therapy. Conclu-sions The modified Zuogui Pill combined with hormone therapy shows better therapeutic effect and safety than hormone therapy on premature ovarian failure.

Objective To discuss the significance of three-dimensional reconstruction as a method of preoperative planning of laparoscopic radiofrequency ablation (LRFA).Methods Thirty-two cases of LRFA admitted from January 2014 to December 2015 in Department of Hepatobiliary Surgery,Chinese People's Liberation Army General Hospital were analyzed (3D-LRFA group).Three-dimensional (3D) reconstruction were taken as a method of preoperative planning in 3D-LRFA group.Other 64 LRFA cases were paired over the same period without three-dimensional reconstruction before the operation (LRFA group).Hepatobiliary system contrast enhanced CT scan of 3D-RFA patients were taken by multi-slice spiral computed tomography(MSCT),and the DICOM data were processed by IQQA(R)-Liver and IQQA(R)-guide to make 3D reconstruction.Using 3D reconstruction model,diameter and scope of tumor were measured,suitable size (length and radiofrequency length) and number of RFA electrode were chosen,scope and effect of radiofrequency were simulated,reasonable needle track (s) was planed,position and angle of laparoscopic ultrasound (LUS) probe was designed and LUS image was simulated.Data of operation and recovery were collected and analyzed.Data between two sets of measurement data were compared with t test or rank sum test,and count data with x2 test or Fisher exact probability test.Tumor recurrence rate was analyzed with the Kaplan-Meier survival curve and Log-rank (Mantel-Cox) test.Results Compared with LRFA group ((216.8±66.2) minutes,(389.1 ±183.4) s),3D-LRFA group ((173.3 ±59.4) minutes,(242.2 ± 90.8) s) has shorter operation time(t =-3.138,P =0.002) and shorter mean puncture time(t =-2.340,P =0.021).There was no significant difference of blood loss (P =0.170),ablation rate (P =0.871) and incidence of complications(P =1.000).Compared with LRFA group ((6.3 ± 3.9)days,(330 ± 102)U/L,(167 ±64) ng/L),3D-LRFA group ((4.3 ± 3.1) days,(285 ± 102) U/L,(139 ± 43) ng/L) had shorter post-operative stay(t =-2.527,P =0.016),less post-operation ALT changes (t =-2.038,P =0.048) and post-operative TNF-αt changes (t =-2.233,P =0.027).Disease-free survival between two groups was significantly different (x2 =4.049,P =0.046).Disease-free survival of 12 months survival rates were 77.6％ and 65.7％ in 3D-LRFA group and LRFA group,respectively.The median disease-free survival was 16.0 months in LRFA group and over 24.0 months in 3D-LRFA group.Conclusions Three-dimensional model of liver reconstruction based on image information is a powerful tool in liver surgery planning.It helps to simulate tumor location and vital tubular structure,make plan for interventional treatment,and therefore mean puncture time and operation time is shortened,influence on liver function is reduced,hospital stay is decreased and DFS is prolonged.

Objective To discuss the significance of three-dimensional reconstruction as a method of preoperative planning of laparoscopic radiofrequency ablation (LRFA).Methods Thirty-two cases of LRFA admitted from January 2014 to December 2015 in Department of Hepatobiliary Surgery,Chinese People's Liberation Army General Hospital were analyzed (3D-LRFA group).Three-dimensional (3D) reconstruction were taken as a method of preoperative planning in 3D-LRFA group.Other 64 LRFA cases were paired over the same period without three-dimensional reconstruction before the operation (LRFA group).Hepatobiliary system contrast enhanced CT scan of 3D-RFA patients were taken by multi-slice spiral computed tomography(MSCT),and the DICOM data were processed by IQQA(R)-Liver and IQQA(R)-guide to make 3D reconstruction.Using 3D reconstruction model,diameter and scope of tumor were measured,suitable size (length and radiofrequency length) and number of RFA electrode were chosen,scope and effect of radiofrequency were simulated,reasonable needle track (s) was planed,position and angle of laparoscopic ultrasound (LUS) probe was designed and LUS image was simulated.Data of operation and recovery were collected and analyzed.Data between two sets of measurement data were compared with t test or rank sum test,and count data with x2 test or Fisher exact probability test.Tumor recurrence rate was analyzed with the Kaplan-Meier survival curve and Log-rank (Mantel-Cox) test.Results Compared with LRFA group ((216.8±66.2) minutes,(389.1 ±183.4) s),3D-LRFA group ((173.3 ±59.4) minutes,(242.2 ± 90.8) s) has shorter operation time(t =-3.138,P =0.002) and shorter mean puncture time(t =-2.340,P =0.021).There was no significant difference of blood loss (P =0.170),ablation rate (P =0.871) and incidence of complications(P =1.000).Compared with LRFA group ((6.3 ± 3.9)days,(330 ± 102)U/L,(167 ±64) ng/L),3D-LRFA group ((4.3 ± 3.1) days,(285 ± 102) U/L,(139 ± 43) ng/L) had shorter post-operative stay(t =-2.527,P =0.016),less post-operation ALT changes (t =-2.038,P =0.048) and post-operative TNF-αt changes (t =-2.233,P =0.027).Disease-free survival between two groups was significantly different (x2 =4.049,P =0.046).Disease-free survival of 12 months survival rates were 77.6％ and 65.7％ in 3D-LRFA group and LRFA group,respectively.The median disease-free survival was 16.0 months in LRFA group and over 24.0 months in 3D-LRFA group.Conclusions Three-dimensional model of liver reconstruction based on image information is a powerful tool in liver surgery planning.It helps to simulate tumor location and vital tubular structure,make plan for interventional treatment,and therefore mean puncture time and operation time is shortened,influence on liver function is reduced,hospital stay is decreased and DFS is prolonged.

OBJECTIVETo investigate the clinical application of expanded flap based on cutaneous branch of transverse cervical artery for reconstruction of cervical cicatricial contracture.

METHODSBased on the clinical anatomy of cutaneous branch of transverse cervical artery flap, we design the corresponding subclavicular area for expansion. The incision was usually located at the anterior axillary fold, 5-8 cm in length. The expander was implanted under the deep fascial layer, without injury of the vascular pedicle. Fixation sutures were put about 1 cm apart from the incision to prevent the expander from transposition and exposure. After expansion, the cervical cicatricial contracture was excised and released. According to the defect, the expanded flap based on the cutaneous branch of transverse cervical artery was designed, with the pedicle located at the posterior margin of sternocleidomastoid and 1. 8 cm above median point of clavicle. "S" shape incision was made at the location of vascular pedicle. Subcutaneous dissection was performed 1.5 cm in width along the incision on both sides. Then the flap was harvested under the deep fascial layer and rotated to cover defect without tension. It was not necessary to dissect the vascular pedicle further. The defect at donor site was closed directly.

RESULTS17 cases were treated with the island flap. The contracture of the cervical scar was corrected completely with aesthetic appearance.

CONCLUSIONSExpanded flap based on cutaneous branch of transverse cervical artery has reliable blood supply. It' s an ideal flap for the treatment of cervical cicatricial contracture.

Arteries ; Cicatrix ; surgery ; Clavicle ; Contracture ; surgery ; Dissection ; methods ; Fasciotomy ; Humans ; Neck ; blood supply ; Neck Muscles ; anatomy & histology ; Surgical Flaps ; blood supply

Objective To investigate the treatment methods of tissue defects in nose and lower eyelid area.Methods Based on the impaired area,combined pre-expansion of forehead and facial region was adopted.The volume of soft tissue expander was determined.150-170 ml expander was implanted between frontalis muscle and periosteum of forehead,while at the facial region,and the volume of expander should be determined by the normal skin area as large as possible.The excision of impaired tissue was performed after sufficient expansion,and then skin flap or transposition skin flap were advanced at the facial region.At forehead,ortho-position skin flap based on supratrochlear vessels was designed for repairing the nose and palpebra inferior region.Three weeks later division of the ortho-position skin flap was carried out.With the flattened pedicle,the rest part of impaired area was replaced.Results Six cases were performed with this method.For all the cases,the survival rate of flap was 100％.Esthetic appearance and satisfying color-match were achieved,without appearance of lower eyelid ectropion.Conclusions Combined pre-expansion of forehead and facial region is an ideal method in treatment of the defects in nose and lower eyelid area.

Objective To explore the repair method for wounds after excision of facial benign tumors.Methods Unilateral or bilateral deltopectoral skin flaps were expanded depending on the area of the facial benign tumor.Expander was implanted underneath deltopectoral flap region through an incision inferior to the clavicle.When expansion was completed,all or part of the benign tumor was excised before designing the flap according to the area of the skin defect.The area of the skin flap should be more than that of skin defect with 10％ to 15％.The pedicle wound could be sealed by rolling it around to form a tube or a hinge using the benign tumor and pedicle.The flap was delayed three weeks later and the pedicle was divided one week after flap delaying.Results All 20 cases got the satisfactory results with treatment of pedicled expanded deltopectoral skin flaps for repair of wounds after excision of facial benign tumor.Conclusions It is a better option to repair a large area wound after excision of facial benign tumor with an expanded deltopectoral skin flap.

Adolescent ; Adult ; Female ; Humans ; Liver Neoplasms ; diagnosis ; surgery ; Male ; Retrospective Studies ; Sarcoma ; diagnosis ; surgery ; Young Adult