Objective:To evaluate the usefulness of Periocheck in clinical application before and after periodontal curettage.Methods:Periocheck was applied in 65 sites in 65 adult patients with periodontitis within 15 min before and after scaling and root planing to monitor peptidase activity produced by Porphyromones gingivalis (Pg),Treponema denticola(Td) and Bacterides forsythus (Bf) in subgingival plaque. The peptidase activity was determined both spectrophotometrically and by using color standard.Results:The peptidase concentrations (Try U/L) before and after treatment were 0.44±0.23 and 0.34±0.26 (P<0.05),while Periocheck positiveness was found in 65/65 and 43/65 (P<0.01) of the patients,respectively.After treatment clinical parameters such as GI, PD,AL and peptidase concentration decreased (P<0.05) in 22 Periocheck positive patients,while those did not (P>0.05) in 43 negitive.Conclusion:Periocheck might provide a promising chair-side monitoring tool for the evaluation of scaling and root planing.

Objective: To determine the distribution of Actinobacillus actinomycetemcomitans (Aa) in subgingival plaques in the patients with chronic periodontitis(CP). Methods: Samples of subgingival plaque were detected for Aa distribution by oligonucleotide probe from 60 sites of CP patients and 10 healthy sites of healthy people.Each sample was obtained from each site of each people. Results: Aa was detected in 19 out of 60 diseased sites(31.67%); Aa wasn't detected in healthy sites.The age(28.68±7.33) of Aa-positive patients was younger than that (44.48±12.48) of Aa-negative patients(P<0.05).Conclusion: Aa is possibly to cause chronic periodontal infection in younger people.

Objective To evaluate the efficacy of radiofrequency ablation combined with absolute ethanol injection in the treatment of hepatocellular carcinoma (HCC), and to study the value of contrast-enhanced ultrasound in the evaluation of therapeutic effect.Methods From May 2015 to May 2017, 60 patients with liver cancer (72 lesions) were treated in our hospital.The patients were divided into 3 groups, the RFA treatment group (n=20), 24 lesions, the PEI treatment group (n=20), 22 lesions, and the RFA+PEI treatment group (n=20) with26 lesions.3 groups of patients were treated with contrast-enhanced ultrasound (CEUS), routine color Doppler ultrasound (CDUS), enhanced CT to evaluate the specific situation of the tumor, and evaluate the therapeutic effect again after operation.The rates of necrosis, lesion inactivation and residue were evaluated in each group.Results The necrosis rate of RFA group, PEI group and RFA+PEI group were66.67%,81.82% and 96.15%, respectively.The necrosis rate of CT group was the highest, and the difference was statistically significant (P<0.05) in RFA+PEI group.Comparison of different imaging to assess the sensitivity and specificity of CDUS, to evaluate the sensitivity and specificity were 81.36% and 53.85%;CEUS to evaluate the sensitivity and specificity were 98.31% and 84.62%, significant difference was statistically significant (P<0.05).Conclusion RFA+PEI treatment program is better than RFA and PEI alone treatment, the treatment effect is better, CEUS in the evaluation of RFA+PEI treatment effect, has higher clinical value.

Objective To explore the imaging features of bone tumor of fibrous tissue origin.Methods 31 patients with bone tumor of fibrous tissue or igin confirmed by pathology were analyzed. All patients were examined by X-ray, of them, 6 by CT in the meantime.Results Non-ossifying fibroma(NOF n=14)occured commonly i n long bone,the imaging findings presented sacculated and expanded bone destruct ion which was encircled by sclerotic borders,bony septum could be observed iusid e the lesion.Ossifying fibroma(OF n=10)occured more in maxillofacial bone,there were circle or elliptical and expansive bone change,and most of them associated with local bone malformation.Depending on the ossified degree,the density of les ion,could be hyperdense,ground-glass opacity,cyst or mixed density,defined borde r,partly associated with sclerotic borders.Desmoplastic fibroma of bone(DF n=1) presented multiple patchy bony erosion at lateral of lower part of femur,and ext ending to articalar facies,ill-defined border,and soft tissue slightly swelling. Fibrosarcoma of bone(FS n=6) occured commonly in long bone,presened bitted-like or greater patchy osseous absorption with soft fissue mass,rarely periosteal rea ction and calcification.58.08% patients(18/31) were accurately diagnosed.The nat ure of 6 patients was undefined.Misdiagnosis was made in 7.Conclusion Most of NOF and OF are of specific imaging fin dings and can be accurately diagnosed before cperation.DF is of slight symptoms with invaded growth and is easily misdiagnosed.In the imaging features of FS are apparently different with various type and histological differentiation,it must be differentiated from other diseases.

Objective To study the specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalian. Method The specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalians were amplified and directly se-quenced. The sequence data of PCR products of human beings and mammalians were declared. Results The sequences of 3'UTR of SON gene of human beings differed from 14 kinds of mammalians. The sequences of samples came from different human individuals did not show any difference. Conclusion The 3'UTR of SON gene is a good DNA region for specie identification. Using this technology, DNA of human beings can be distinguished from 14 kinds of mammalians. This technology is very useful in of forensic science.

In this study the umbilical artery and uterine artery blood flow was measured by Acuson 128 color computer ultrasound in 90 healthy pregnant women, and the reference ranges and mathematical models of its indices were established. The variation of observers and measurement time and the effect of different areas of the same vessel on measured blood flow values were also studied. The results show reducing of resistance and increasing of blood flow of the umbilical and uterine arteries in healthy pregnancy with increased gestational age. Doppler blood flow measurement has a good repeatability and is worth while to be used universally. The Doppler signals shound be recorded from near placenta part of umbilical artery and upper branch of uterine artery in a side near to placenta.

Objectives To learn the bednet use and influential factors among Wa and Lahu ethnics,and further to explore feasible ways of promoting the use of bednets and insecticide-treated nets (ITNs). Methods Questionnaire surveys and statistical analysis with EpiInfo 2000 software package were carried out. Results Only did 2.8% [95% confidence intervals(95%CI):1.2%-5.4%] of Wa and 4.8 % (95%CI:2.8%-8.1%) of Lahu ethnic members use bednets frequently or sometimes. Bednets were not available for 67.7% (95% CI:62.0%-73.1%) of Wa and 59.7% (95% CI:53.1%- 66.1%) of Lahu ethnic peoples. Most people who had bednets did not often use them for anti-mosquitoes. Results of multivariate logistic regression confirmed significant correlation between bednet use and knowledge of malaria prevention. Conclusion The coverage and use rates are very low in the two ethnical minorities. Health education, communication and social marketing are the strategies for promoting the usage of bednet and ITNs.

Objective To establish a new method for determination of copper, nickel, silver, lead, cadmium and mercury in water with solid phase extraction followed by rapid high performance liquid chromatography. Methods The copper, nickel, silver, lead, cadmium and mercury ions were pre-column derived by tera-(4-dimethylaminophenyl)-porphine (T4-DMAPP). These chelates were enriched by solid phase extraction with C18 cartridge and the 100 times enriched products of were obtained. The chelates were separated on a ZORBAX Stable Bound (4.6 mm?50 mm, 1.8 ?m) by gradient elution with methanol and THF as mobile phase at a flow rate of 2.0 ml/min and detected with photodiode array detector from 350-600 nm. Results The detection limits of nickel, copper, silver, lead, cadmium, and mercury were 3, 2, 4, 3, 1.5 and 3 ng/L respectively, the recovery rates were 92%-104%, relative standard deviations were 2.8%-3.5%. Conclusion This method can be applied to the determination of nickel, copper, silver, lead, cadmium, and mercury in water with good results.

BACKGROUND: It is indicated in researches of recent years that both astragalus and angelica act on anti-free radical and protect renal injury due to ischemia / reperfusion.OBJECTIVE: To observe the protection and its mechanism of astragalus and angelica injections on adenosine triphosphate-ase (ATPase) in renal injury due to ischemia/reperfusion.DESIGN: The observing controlled experiment based on experimental animals .SETTING: Physiological teaching & research room and teaching & research room of renal functional protection in a medical college. MATERIALS: The experiment was performed in Physiological Experimental Room of Luzhou Medical College from January 2001 to March 2001. Totally 33 Japanese big-ear white healthy adult rabbits of either sex were employed,provided by Experimental Animal Center of Luzhou Medical College, in the mass of(1.63 + 0. 22) kg. According to random number table, they were divided in sham-operation control(8 rabbits), simple ischemia/reperfusion group (8 rabbits), astragalus injection + ischemia/reperfusion group (astragalus group) (8 rabbits) and angelica injection + ischemia/reperfusion group(angelica group) (9 rabbits).METHODS: One day before operation, on the day of operation and 1 day after operation, successively, intravenous medical injections (astragalus 1.25 g/kg,angelica 12.5 g/kg) were administrated in astragalus and angelica groups everyday respectively, and injection with physiological saline 5 mL/kg was applied in the control and simple ischemia/reperfusion group. In 48 hours reperfusion after 1 hour ischemia in kidney, blood sample was collected from inferior vena cava. The upper tissue of the right kidney was collected and fixed by placed in 30 mL/L glutaraldehyde and the lower tissue was prepared into homogenate. Ultrastructure of renal tissue was examined with electron microscope; serum creatinine level and ATPase activity in renal tissue were assayed.MAIN OUTCOME MEASURES: Ultrastructure of renal tissue, serum creatinine level and ATPase activity in renal tissue.RESULTS: In simple ischemia/reperfusion group, renal tissue was degenerated significantly, and the disorders in astragalus and angelica groups were reduced markedly compared with simple ischemia/reperfusion group. Serum creatinine level in simple ischemia/reperfusion group was higher remarkably than the sham-operation control ( P ＜ 0. 05 ), and that in astragalus and angelica groups was reduced than simple ischemia/reperfusion group (P ＜ 0. 05) . In simple ischemia/reperfusion group, the levels of Mg2+-ATPaes, Na+-K+-ATPase and Ca2+-ATPase were(0. 155 ±0. 020),(0.179±0.018), (0.150±0.022) nkat/g respectively, which was markedly reduced compared with sham-operation control [ (0. 174 + 0. 012),(0. 198 + 0. 012), (0. 181 + 0. 017) nkat/g], ( t = 2. 344, 2. 438, 3. 014,P ＜ 0.05 ). In astragalus and angelica groups, respectively, the activities of Mg2+-ATPaes, Na+-K+-ATPase and Ca2+-ATPase were(0. 172 ± 0. 023),(0. 196 ±0. 077), (0. 175 ±0. 016) and (0. 177 ±0. 015), (0. 200 ±0.011 )and (0. 181 ± 0. 025) nkat/g successively. Except that Mg2+-ATPaes activity in astragalus group was not different significantly from that in simple ischemia/reperfusion group, all the rest were higher than simple ischemia/reperfusion group(t =2. 372 -2. 786, P ＜0.05).CONCLUSION: Both astragalus and angelica inhibit the decrease of ATPase and improve the disturbed local blood-flow adjustment in kidney, which has provided experimental basis of astragalus and angelica on reducing renal injury induced by ischemia/reperfusion through protecting ATPase.

Objective To explore the effect of recombinant human tumor necrosis factor-α receptor Ⅱ:IgG Fc fusion protein injection (rhTNFR:FC) on the expression of cartilage oligomeric matrix protein (COMP) in the synovial fluid and peripheral blood of juvenile idiopathic arthritis (JIA); and to explore the clinical significance of COMP for JIA and the relationship between rhTNFR:FC and COMP in JIA.Methods Thirty-five patients with JIA (JIA group),30 patients with traumatic arthritis (trauma group) and 30 patients with indirect inguinal hernia hernioplasty (normal group) were included.Peripheral blood from all enrolled patients and synovial fluid from 15 JIA and 10 trauma arthritis were obtained for COMP detection before the treatment.Fifteen JIA (group A) patients were treated with combined rhTNFR:FC,diseasemodifying antirheumatic drugs (DMARDs) and non-steroid anti-inflammatory drugs (NSAIDs),20 JIA (group B) were treated with combined DMARDs and NSAIDs.After three to six months' treatment and when the disease were in remission,peripheral blood from group A and B were drawn for COMP detection.In group A,the synovial fluid from 5 patients were obtained for COMP detection after treatment.At the same time,such as tender joint count (TJC),swollen joint count (SJC),time for morning stiffness,blood routine,erythrocyte sedimentation rate (ESR),and C-reactive protein (CRP) and other parameters before and after treatment were measured.The level of COMP was tested by double antibody sandwich enzyme-linked immunosorbent assay.The measurement data were tested for variance and independent sample t-test; and the enumeration data were tested by chi-squared or Fisher's exact test.Pearson's correlation analysis was adopted to analyze the association among the variables.Results ① The blood COMP level before treatment was (0.77±0.29) ng/ml in the JIA group,(1.00±0.28) ng/ml in the traumatic arthritis group,and (1.33±0.37) ng/ml in the normal control group.The level in the former two groups was obviously lower than that in the normal control group.The variation was statistically significant (F=25.345,P＜0.05).The comparison between any two groups was statistically significant (P＜0.05).② The COMP level in the synovial fluid before treatment were (14.8±1.6) ng/ml in the JIA group,(15.1±1.0) ng/ml in the traumatic arthritis group.The variation was not stati-stically significant (t=0.523,P=0.606).③ The serum COMP level of the systemic JIA group was obviously lower than that of the oligoarticular JIA patients,and patients with enthesitis-related arthritis and polyarticular JIA (0.26± 0.03 vs.0.87±0.17,0.89±0.22 and 0.70±0.35 ng/ml,respectively; F=9.244,P＜0.05).④ The serum COMP level of JIA at the acute phase was negatively correlated with white blood cells count (WBC),CRP and ESR (r=-0.556,-0.582 and-0.684,respectively; P all＜0.05).By contrast,no correlation was detected between the serum COMP level and joint tenderness index,joint swelling index,morning stiffness duration,hemoglobin level and platelet count(r=0.06,-0.206,-0.107,0.15 and-0.185,respectively; P all ＞0.05).⑤ The serum COMP level was obviously lower in the JIA with joint destruction than that without joint destruction (0.52±0.22 vs.0.92±0.22 ng/ml; t=5.207,P＜0.05).⑥After treatment,the blood COMP level in group A was (1.33±0.21) ng/ml and (0.96±0.22) ng/ml in group B,which was obviously higher than that in the JIA group before treatment (0.77±0.29) ng/ml.In addition,the level in group A was higher than that in group B.The variation was statistically significant (F=24.681,P＜0.05).⑦ After treatment,the COMP level in the synovial fluid (18.4± 1.1) ng/ml (n=5) was higher than that before the treatment was (14.8± 1.6) ng/ml (n =15).The variation was of statistical significant (t=4.565,P＜0.05).Conclusion The COMP level in blood and synovial fluid declines before treatment and increases after treatment.The increase is more obvious after combined with rhTNFR:FC treatment.The serum COMP level is remarkably decreased in JIA at the acute phase,systemic JIA,and the JIA with destruction of joint,and showes a negative correlation with WBC,CRP and ESR.Serum COMP may be a useful marker of active disease,destruction of joint and growth inhibition for patients with JIA.rhTNFR:FC treatment for JIA can facilitate the recovery of COMP.