Objective To observe the clinical effect of surgery combined with traditional Chinese medicine in the treatment of sacrococcygeal pilonidal sinus.Methods Retrospective analysis of clinical characteristics of 18 patients with sacrococcygeal pilonidal sinus was conducted.Results 18 cases of sacrococcygeal pilonidal sinus with recurrent infection,five cases of whom were misdiagnosed in other hospitals;18 patients were treated with surgery,pathological diagnosis showed foreign body granuloma,which were coincidence with the diagnostic criteria of pilonidal sinus.All of these were treated with Chinese herbal fumigation and washing of clearing away heat,eliminating swell,drying dampness,dntipruritic effect and promoting granulation.No recurrence was found in follow-up of 6 months to 3 years.Conclusion Pilonidal sinus is easy to misdiagnosis,and extensive excision combined with traditional Chinese medicine are the key to prevent recurrence.

Large Hiatal Hernia(LHH)is a special type of hiatal hernia,usually defined as more than 1/3 of stomach mi-gration with or without other organs or diameter of esophageal hiatus more than 5 cm.Operation is the only therapeutic method. With the development of laparoscopic surgery, laparoscopic repair of large hiatal hernia(LRLHH)is the standard technique for repair of large hiatal hernias.However LRLHH still is a kind of complexity, difficulty, high risk and recurrence to some extent. Moreover, there is still no consensus amongst surgeons about what is the optimal technique for LRLHH .Currently, controver-sies and advancement of LRLHH mainly concentrated on completely dissection and reduction of the sac from the mediastinum , suture cruroplasty with or without mesh, deal with short esophagus and gastroesophageal reflux.Modified LRLHH achieves completely dissection hiatal hernia sac , which is more convenient and more helpful for suture cruroplasty and reduce the ten-sion.The use of mesh effectively reduces the anatomical recurrence rate and may lead to complications such as esophageal ero-sion or extensive fibrosis with troublesome dysphagia .LRLHH with mesh or without mesh should be individualized .Optimal technique for LRLHH needs continual improvement and high-quality randomized controlled trials to obtain the most effective and safe mesh in the long term.In this paper, the controversy and progress of laparoscopic huge esophageal hiatal hernia repair will be summarized.

Objective To observe the clinical effect of the treatment of intestinal rupture with peritonnitis. Methods The clinical data of 16 patients with hernia and intestinal rupture complicated with peritonitis who were treated by primary repair surgery were analyzed retrospectively.In the operation,the full debridement,cleaning,and the operation of a hernia repair,postoperative drainage irrigation.Results 16 cases were good and no recurrence after operation.Conclusion It was reliable to use prin ary repair for the treatment of intestinal rupture with peritonitis and incarcerated hernia.

Objective:To study daily maintenance and quality control of high pressure steam sterilizer in the hospital to provide effective methods and suggestions for medical equipment maintenance.Methods: 6 high-pressure steam sterilizers were selected and divided into three groups according to the brands and the departments which use them, in order to compare the data, analyze equipment failure, improve the maintenance process and control the risks.Results:The equipment failure rate reduced lower than 21% through the improvement of maintenance. And the equipment utilization rate was above 95% through improving personnel training. Conclusion: The pertinence of the medical equipment maintenance and training is of great significance to carrying out effective regular maintenance of equipment by the office personnel, and it can reduce the failure rate and ensure the equipment operate safely.

Objective　The microvasculature of the anterior lobe, posterior lobe and cerebellar vermis was observed under the optical microscope in 6 rats. Methods　The tannic acid-ferric chloride method (TAFM) was used to stain the vessels in cerebellum. Results　The arteries within the cortex and medulla of the cerebellum originated from pia mater arteries and central branches of the cerebellar arteries, the branches of pia mater arteries mostly penetrated into the cerebellar cortex vertically and formed capillary net in the molecular layer, purkinje layer and granular layer, the arteriole in the medulla of cerebellar posterior lobe branched and formed clawed-like figurations. These vessels beneath lobule cortex connected mutually by vessel anatomosis across the medulla. Conclusion　The arteriole in the cerebellum can be manifested distinctly in a strong three-dimension by TAFM and the vessel anatomosis in deep layer of cerebellum can be easily observed.

Objective To explore the significance and value of Kisspeptin in the diagnosis and therapeutic evaluation for idiopathic central precocious puberty(ICPP) in girls.Methods Twenty-four girls with ICPP and 21 girls with premature thelarche(PT) who were hospitalized in the Children's Hospital of Jiangxi Province from Jun.2012 to Jan.2013 were selected as ICPP group and PT group,and 25 healthy girls were selected as healthy control group.The coagulation vein blood in ICPP girls before treatment and after 6 months treatment and PT girls and healthy girls were collected,and enzyme linked immunosorbent assay was used to detect the plasma Kisspeptin level,and t test was used to analyze the differences among the 4 groups.Results The Kisspeptin level of girls with ICPP [(1.80 ± 0.13) μg/L]was apparently higher than that of PT groups [(1.41 ± 0.10) μg/L] and healthy control group[(1.39 ± 0.13) μg/L],and the differences were statistically significant (t =10.974,14.787,all P =0.000).However,the difference of Kisspeptin between PT group and healthy control group was not statistically significant(t =10.970,P =0.095).In addition,the Kisspeptin level of ICPP girls who undewent 6-month treatment [(1.49 ± 0.15) μg/L] was significantly lower than that before treatment,and the difference was statistically significant (t =10.80,P ＜ 0.05) ;but,compared with PT group and healthy control group,there was no significant difference (t =6.32,P =0.060 ; t =7.44,P =0.214).Conclusions Kisspeptin level is related with pubertal development,and it can be used as an important evidence in ICPP diagnosis and an important parameter in ICPP therapeutic evaluation.

AIM: A novel method for the overall quality control of Herba Artemisiae scopareiae has been established by HPLC fingerprint. METHODS: The chromatographic fingerprints were obtained by injecting ~5 ?L of the sample solution each time on a Kromasil ODS column (250 mm?~4.6 mm ,~5 ?m ) with the gradient elution solvent system composed of 1%acetate acid-water-methanol. The flow rate was 1 mL/min, the column temperature was maintained at ~30 ℃ and the detection wavelength was set at ~326 nm . RESULTS:22 co-possessing peaks were selected as the fingerprint peaks of Herba Artemisiae scopareiae and the similarities between the chromatographic fingerprints of the herbal drugs were calculated taking chlorogenic acid peak as the reference peak. The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index (F), the relative index (Fr) and the fingerprint components apparent prercentage contents (P%). CONCLUSION: This method with good precision and reproducibility can be useful in the quality control of Herba Artemisiae scopareiae.

Objective To detect the relationship between ecNOS gene polymorphism and ischemic stroke in Dalian,China.Methods The polymorphism of intron 4 of ecNOS gene was analyzed in normal group(90 cases) and ischemic stroke group(170 cases) by a combination of polymerase chain reaction(PCR).Results In ischemic stroke group,hypertension, diabete mellitus, smoking, fibrinogen and internal carotid artery narrow were significantly different from those of control group(all P

Sleep is a vital phenomenon of life,the disturbance of which could be associated with a wide range of diseases,such as depression,anxiety,memory loss and hypertension. After years of efforts,pharmacological research of sleep in China has been in line with the pace of international sleep study,especially in the mechanisms of sleep. Our research is beginning to involved in several hot spots of study,such as the relationship between sleep disorders and their comorbidities(PTSD, depression,hypertension,diabetes and neurodegenerative diseases). Attention has also been paid to the research and discovery of novel hypnotic drugs. Despite the gap in sleep research between China and other developed countries,sleep study in China will definitely step into a gold period as well as solve sleep problems for more patients as soon as possible with the joint efforts of researchers and with the increasing attention to healthy sleep.