Objective:To explore the SPECT detection method of quality control in detection of main performance index. Methods: according to the 2007 edition of SPECT performance measurementof the NEMA (the United States Electrical Manufacturers Association) standard, and the corresponding model of intrinsic spatial resolution, intrinsic spatial non-linearity, intrinsic non-uniformity, characteristic count rate, system planar sensitivity, system spatial resolution, fault spatial resolution and other indicators. Results: after quality control testing, have deviation more index hospitals SPECT results. Conclusion: the hospital should detect quality control as far as possible in nuclear medicine and nuclear medicine imaging equipment, ensure the normal operation of equipment, and improve the quality of image.

Objective: To study the new device and technology in prepartion of superfined Chinese herbal medicine powders. Methods: Improving the Trost jet mill was to divide continually the feed and the coarse grains classified from a circular disc into two two phase flow by means of a especial four way pipe in mixing. and to impact with high speed oppositely each other, the superfined powders are prepared. Results: The superfined powders of ginseng, boat fruited sterculia seed and balsam pear are successfully prepared to guarantee 10～15?m in diameter. Conclusions: It is very effective to pulverize Chinese herbal medicine with this device in mass production.

Objective To evaluate the monitoring value of serum albumin and plasma von willebrand factor in patients with stroke.Methods Serum albumin and plasma von willebrand factor were measured from 145 patients with stroke(95 patients with nonpoor outcome and 50 patients with poor outcome) in two weeks and after six months from stroke onset to study the relationships with prognosis.Results Serum albumin level was significantly higher in patients with nonpoor outcome(mRS≤2) after six months stroke onset than those with poor outcome(mRS≥3) and was lower after six months stroke onset than in two weeks from stroke onset.On the other hand,plasma vWF level in patients with stroke was significantly higher than that in healthy controls and was higher in two weeks than after six months from stroke onset.Patients with poor outcome had higher plasma vWF level than patients with nonpoor outcome in two weeks and after six months from stroke onset,but had no significant difference.Conclusion The level of serum albumin and plasma von willebrand factor is related with nutritional status,active extent and prognosis in patients with stroke.

Extracorporeal membrane oxygenation(ECMO) is a kind of extra life support technique that can support cardiac and pulmonary function in a relatively long time.With the application of nitric oxide,pulmonary surfactant and high frequency ventilation,the use of ECMO in neonatal respiratory failure decreased.Although received these treatment,there are some newborn with respiratory failure still required ECMO at last.On this paper,the application of ECMO in neonatal respiratory failure from foreign medical institute was introduced,and compared with the domestic situation,in order to improve the application of ECMO in neonatal respiratory failure.

Objective To investigate the situation of oxygen supplement and the incidence and clinical characteristics of long-term oxygen inhalation newborns in neonatal intensive care unit(NICU).Methods The records of oxygen supplement and the related clinical data of 12 155 neonates admitted in our NICU from Oct 2009 to May 2011 were collected and retrospectively analyzed.The results were compared with the data from a survey on 19 hospitals in China which reported by other authors.Results In 12 155 newborns,4 951 were full term,7 204 were preterm.One hundred and two patients (0.84％,102/12 155 ) accepted oxygen for more than 28 days.Among them,88 were preterm,14 were full term,with the average gestational age (31.16 ±3.70) weeks,the average birth weight (1.60 ±0.68) kg and the mean oxygen supplement period (40.60 ± 12.25) d.Finally,98 were cured or improved,4 died.The incidence of bronchopulmonary dysplasia (BPD) in 7 204 preterm infants was 1.22％ ( 88/7 204) according to the standard of continuous oxygen supply more than 28 days after birth.The incidence of BPD in preterm infants less than 32 weeks was 4.92％ (68/1 381 ) according to the standard of continuous oxygen supply more than 28 days after birth,while the rate was only 2.10％ (29/1 381 ) according to the standard of continuous oxygen supply more than 36 weeks postmenstrual age.The rates of BPD according to the two different standards were significantly different ( x2 =16.251,P ＜0.001 ).There were significant differences in the rate of supply oxygen( x2 =119.99) and supply oxygen time( F =109.27 ) among different gestational age groups in overall the 5 499 neonates ( P ＜0.001 ),but no significant differences in the average time of oxygen supply and mechanical ventilation among different gestational age groups in infants with long-term oxygen dependence ( P ＞ 0.05 ).There were significant differences in rates of pulmonary surfactant therapy,heart failure,retinopathy of prematurity,congenital heart disease,other congenital malformation and mortality among different gestational age groups in long-term oxygen dependence infants (x2 =8.789,13.538,23.176,7.778,8.842,8.246,P ＜ 0.05 ).As compared with the data from 19 hospitals,the corrected rate of long-term oxygen supplement in preterm infants in our hospital was obviously lower[0.99％ (71/7204) vsl.54％ (190/12 351),P ＜0.001].Conclusion Theincidence of BPD in our NICU is low.Lower gestational age,immature lung and secondary lung injury may be the mainly cause of neonatal long-term oxygen dependence,but some factors such as congenital heart disease,congenital malformations should be considered in more mature infants.The most appropriate standard for BPD still remains to be discussed.

Objective: The present study aimed to isolate and characterize a cancer stem cell-like sphere-forming cell subpopula-tion. Methods: By using a spheroid culture stem cell-conditioned medium, we isolated a subgroup of cancer stem-like cells from naso-pharyngeal cancer cell lines. Chemotherapy resistance was analyzed by using methyl thiazolyl tetrazolium, and clone-forming capabili-ty was determined by using softer agar clone formation assay. Finally, we verified the expression of the stemness-specific gene andβ-catenin by using immunocytochemistry staining and RT-PCR. Results: The lower-differentiated nasopharyngeal cancer lines con-tained more sphere-forming cells, whereas sphere-forming cells were not observed in the high differentiated nasopharyngeal cancer line CNE-1. Compared with CNE-2, CNE-2S (sphere-forming cells derived from CNE-2) exhibited higher chemotherapy resistance and clone-forming ability. Interestingly, the stemness genes BMI-1, ABCG2, and ALDH1 exhibited higher expression in CNE-2S than in CNE-2. β-catenin, a vital transcription factor of the WNT pathway related to stem cells, exhibited higher expression in CNE-2s cellular nucleus and plasma than in CNE-2. Conclusion: We isolated a subgroup of stem-like nasopharyngeal cancer sphere-forming cells. This discovery paves the way for the development of therapeutic strategies aimed at eradicating tumorigenic subpopulations in nasopharyn-geal cancer.

OBJECTIVETo systemically investigate receptor activator of nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) induced differentiation of osteoclasts.

METHODSMouse protein-protein interaction(PPI) database NIA and published microarray dataset GES16749 were used to construct and analyze PPI network of RANKL and M-CSF induced mouse monocyte RAW264.7.

RESULTSIn the PPI network, transforming growth factor beta receptor 1 (TGFBR1), Rous sarcoma oncogene (SRC), myelocytomatosis oncogene(MYC) and integrin beta 3 (ITGB3) were able to interact with more proteins and they were the key nodes in the signaling transduction.

CONCLUSIONTGFBR1, SRC, MYC and ITGB3 might be the key points of RANKL and M-CSF induced differentiation of osteoclasts.

Animals ; Carrier Proteins ; Cell Differentiation ; Macrophage Colony-Stimulating Factor ; Membrane Glycoproteins ; Mice ; Osteoclasts ; Protein Interaction Maps ; RANK Ligand ; Receptor Activator of Nuclear Factor-kappa B

Objective To explore the effect of triglyceride glucose(TyG)index and uric acid level on carotid athero-sclerosis in patients with type 2 diabetes mellitus(T2DM),and build a risk nomogram model for T2DM complicated with carotid atherosclerosis.Methods A total of 125 T2DM patients admitted to the First People's Hospital of Xinxiang City from January 2020 to December 2022 were selected as the research subjects.The patients were divided into the carotid atherosclerosis group(n=33)and the non-carotid atherosclerosis group(n=92)according to whether they had carotid atherosclerosis.The general clinical data such as gender,age,course of disease,body mass index(BMI),diastolic blood pressure(DBP),and systolic blood pressure(SBP)of patients were collected through the electronic medical record system.The levels of triglycerides(TG),fasting plasma glucose(FPG),creatinine(Cr),fasting insulin(FINS),glycosylated hemoglobin(HbA1c),high-densi-ty lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),and total cholesterol(TC)were measured by using a fully automatic biochemical analyzer.The uric acid level was determined by using the uricase method.The risk factors of carotid atherosclerosis in T2DM patients were analyzed through univariate and multivariate logistic regression.R3.5.3 software was used to draw a nomogram model to predict carotid atherosclerosis in T2DM patients.The predictive performance of the nomogram model was validated by the receiver operating curve(ROC),and the accuracy of the model was tested by the Bootstrap method.Additionally,a total of 27 T2DM patients admitted to the First People's Hospital of Xinxiang City from January 2023 to June 2023 were selected to validate the predictive performance of the model.Results The univariate analysis showed that there were significant differences in the age,course of disease,Cr,FINS,FPG,TC,TG,TyG index and uric acid level of patients between the carotid atherosclerosis group and the non-carotid atherosclerosis group(P＜0.05);there was no statisti-cally significant difference in gender,BMI,DBP,SBP,HbA1c,HDL-C and LDL-C of patients between the two groups(P＞0.05).Multivariate logistic regression analysis showed that age ≥60 years,high Cr level,low FINS level,high TC level,high TyG index and high uric acid level were the risk factors for carotid atherosclerosis in T2DM patients(P＜0.05).The.The validation results of Bootstrap method for validating the early warning model established based on age,Cr,FINS,TC,TyG index and uric acid level to predict the risk of carotid atherosclerosis in T2DM patients showed that the C-index of the model was 0.762(95％confidence interval:0.728-0.808),and the area under the ROC curve,sensitivity,and specificity were 0.779,77.34％,and 82.46％,respectively.Among the 27 T2DM patients,8 cases developed carotid atherosclerosis,while the risk nomogram model predicted 7 cases of carotid arteriosclerosis.The ROC curve showed that the area under the curve,sensitivity and specificity were 0.785,80.47％and 75.36％,respectively.Conclusion High TyG index and high uric acid level are risk factors for T2DM patients with carotid atherosclerosis.The risk nomogram model constructed based on TyG index,uric acid level,age,Cr,FINS and TC has a good predictive effect on carotid atherosclerosis in T2DM patients.

Objective To explore the value of probe-based confocal laser endomicroscopy(pCLE)in the differential diagnosis of fundic gland polyps.Methods A total of 63 patients who underwent conventional white light image(WLI)and suggested with gastric polyps in our gastroenterology endoscopy center during June and December 2023 were enrolled,and their clinical data,WLI,narrow band images(NBI),pCLE images and postoperative histopathological findings were collected.The characteristics of fundic gland polyps under pCLE were statistically verified,and the sensitivity,specificity,accuracy and consistency of different endoscopic diagnoses were calculated respectively.Results Under pCLE,fundic gland polyps were mainly manifested as elongated gastric pit opening,"hand-in-hand"gastric pit opening,well-arranged blood vessels around the pit opening,and no luciferin oozing.The sensitivity,specificity,accuracy of WLI were 72.73％,75.00％,73.13％and 0.343 for fundic gland polyps,and 81.82％,71.43％,71.64％and 0.354 for hyperplastic polyps,respectively.While,the above values of NBI were 87.27％,83.33％,86.57％and 0.673 for fundic gland polyps,and 81.82％,85.71％,85.07％and 0.554 for hyperplastic polyps;and those of pCLE were 92.73％,91.67％,92.53％and 0.769 for fundic gland polyps,and 90.91％,94.64％,94.03％and 0.797 for hyperplastic polyps pCLE had better sensitivity,specificity and accuracy than WLI and NBI,and had the results consistent with histopathological findings.Conclusion pCLE can be used in the differential diagnosis of fundic gland polyps.

Objective:To explore the diagnosis and clinical characteristics of congenital meningocele combined with type 1 neurofibromatosis (NF1).Methods:Clinical data of a child with congenital meningoencephalocele complicated with NF1 diagnosed and treated by the Hunan Provincial Brain Hospital on July 12, 2021 were collected, and relevant literature was reviewed.Results:Patient, female, 9 years old, with milk coffee stains on the skin of the trunk and limbs, mostly with a length greater than 1.5 cm; A three-dimensional CT scan of the skull showed a skull defect in the left temporal occipital region (approximately 7.5 cm×6.7 cm) and below the right posterior occipital tuberosity (approximately 3 cm×3 cm). The patient′s clinical whole exome sequencing detected one pathogenic mutation (NF1: p. Arg681Ter), and underwent lumbar cistern drainage, cyst resection, occipital cistern ostomy, and titanium mesh repair and shaping surgery. The pathological result showed proliferation of arachnoid and fibrous tissue, and good postoperative recovery.Conclusions:Congenital meningoencephalocele combined with NF1 is extremely rare, and early surgery has more advantages than disadvantages. The use of titanium mesh with sliding groove can alleviate the limitation on skull growth to a certain extent. NF1 has diverse clinical manifestations and different onset ages, and should be followed up by a multidisciplinary team for a long time.