TLC scanning technique was, developed for the determination of matrine in Jieshenbao lotion. The method is simple, and highly sensitive, with an average recovery of 99.87%and a variation cofficient of 1.33%.

Objective To develop a HPLC method for the determination of protopine in Rhizoma Corydalis Decumbentis Capsules. Methods A HPLC was performed on a Hypersil ODS column( 4.6? 250 mm, 5 ? m) . The mobile phase was methanol - 0.1 % water solution of triethylamine (65∶ 35). Determination wavelength was 290 nm and flow rate was 1.0 mL/min. The theoretical plates should over 4000 according to the content of protopine. Results A good linearity was shown in the range of 0.3168～ 1.5840 ? g, r=0.9996. The average recovery was 100.6 % , RSD=2.68 % . Conclusion The method is simple, effective and can be used for the quality control of Rhizoma Corydalis Decumbentis Capsules.

Objective To develop a HPLC method for the determination of ephedrine hydrochloride in Xiaochuanning capsules.Methods A Hypersil ODS column was used.The mobile phase was acetonitrile-water solution of 0.05 mol / L NaH2PO4(pH2.7,22 ∶ 78).The flow rate was 1.0 mL / min and column temperature was 40 ℃.Detcted wavelength was 205 nm.Results A good linearity was in the range of 0.1005 ~ 0.5025 ?g(r = 0.9993).The average recovery was 103.1 %,RSD=1.81 %.Conclusion The method is simple,accurate,and can be used for the quality control of Xiaochuanning capsules.

AIM: To establish a HPLC method for determining of ginsenoside Re,ginsenoside Rg_1 and schisandrin in Huoliyuan Tablets(total ginsenoside,Radix Astragali,Fructus Schisandrae Chinensis,Radix Ophiopogonis,etc.). METHODS: A Hypersil C_(18) column was used.The mobile phase for ginsenoside Re and Rg_1 was acetonitrile-water mixed with 0.05 mol/L NaH_2PO_4(pH2.7,20∶80),and determination wavelength was 203 nm.The mobile phase for schisandrin was acetonitrile-0.1%H_3PO_4(48∶52),and determination wavelength was 250 nm. RESULTS: The linear ranges of ginsenoside Re,Rg_1 and schisandrin were 1.84-14.72 ?g(r=0.999 4),1.49-11.92 ?g(r=0.999 0) and 0.094-0.47 ?g(r=0.999 9),respectively.The average recoveries of ginsenoside Re,Rg_1 and schisandrin were 99.86%(RSD=1.69%),98.17%(RSD=2.03%) and 101.54%(RSD=(2.62%),) respectively. CONCLUSION: The method is simple,accurate,and can be used for the quality control of Huoliyuan Tablets.

TLC scanning technique was used for the determination of tanshinone ⅡA in "Prostato Healthcare Bag" at ?S=470nm, and ?R = 620nm. This method is simple and highly sensitive, with an average recovery of 97.45％ and a variation coeffioient of 1,28%.

OBJECTIVE To establish a method of real-time PCR for detection of mecA gene in meticillin-resistant Staphylococcus aureus(MRSA).METHODS The experiment conditions including Mg2+ concentrations,primers concentrations and probe concentrations were optimized for real-time PCR in the light of factorial design principle.Totally 109 strains of S.aureus were collected and detected respectively in oxacillin disk diffusion method and real-time PCR and MRSA detection rate in the two methods were compared and analyzed.RESULTS Thirty seven MRSA isolates were detected by real-time PCR from the 109 S.aureus strains.The MRSA isolating rate by real-time PCR was conspicuously higher than oxacillin disk diffusion method,by which 27 MRSA isolates were detected.CONCLUSIONS The technology of real-time PCR to rapid identification of MRSA is superior to common PCR and it can make up the deficiency of detecting borderline-resistant strains in oxacillin disk diffusion method.

OBJECTIVE To establish and evaluate detection method of meticillin-resistant Staphylococcus aureus(MRSA) by fluorescence in situ hybridization(FISH) and flow cytometry.METHODS The experiment conditions including bacteria suspension concentrations,probe concentrations,and hybridization temperatures were optimized for FISH test in the light of orthogonal design principle.The fluorescence signals were detected by flow cytometer,and detection results by FISH and flow cytometry were compared with that of real-time PCR.RESULTS The optimal hybridization conditions of FISH detecting MRSA were 40?105 of bacteria suspension concentrations per microliter of hybridization buffer solution,and 4 ng of probe concentrations per microliter of hybridization buffer solution and 50 ℃ of hybridization temperatures.The sensitivity and specificity of detecting MRSA by FISH-FCM were 97.3% and 100.0%,respectively,and detection results between FISHFCM and real-time PCR had not significant deviation.CONCLUSIONS Fluorescence in situ hybridization and flow cytometry,which detect directly mecA gene in MRSA, are a fast and accurate methods for MRSA detection and can apply in clinical laboratory.

The special environmental features of high altitude, such as hypobaric hypoxia, low temperature, arid, high solar radiation, variable climate and geochemical anomaly, cause great effects on human physiology and health. It will provide valuable references and new ideas to study drug's metabolism in special environment of high altitude hypoxia, and give the guidance to clinical reasonable medication, avoiding adverse reactions and personalized medicine in plateau areas. This article reviewed the effect of high altitude hypoxia on drug metabolism, elaborated metabolic characteristics of some drugs and the activity and expression of drug metabolism enzymes under hypoxia environment at high altitude, and discussed related mechanism.

AIM: To establish the HPLC method for determinating syringin and betaine in Qianglining Tablet(Radix et Rhizoma seu Caulis Acanthopanacis senticosi,Fructus Lycii). METHODS: A Hypersil C_18 column was used for the determination of syringin.The mobile phase for syringin was methanol-0.1 mol/L acetic acid (24∶76),and the detection wavelength was at 270 nm.A Hypersil NH_2 column was used for the determination of betaine.The mobile phase for betaine was acetonitrile-water(25∶75),and ELS detector was used. RESULTS: The linear range of syringin and betaine were 0.121 6-0.608 0 ?g(r=0.999 9) and 0.660 8-3.964 8 ?g(r=0.999 1),respectively.The average recoveries of syringin and betaine were 99.09%(RSD=2.13%) and 96.34%(RSD=1.31%),respectively. CONCLUSION: The method is simple,accurate,and can be used for the quality control of Qianglining Tablet.

Objective To develop a simple mobile lead room to reduce the scattered radiation to adjacent patients and medical staffs. Methods The triple ply, lead protection sheet, lead glass stainless steel plate, hinge, rotating wheel and the plank were used to build the lead room. Results The dose of the scattered radiation to adjacent patients and medical staffs was decreased and the expenditure of the hospital on the lead room was saved.