Objective:To explore the ability of osteo-articular allograft of variant deproteinized bone combining calcium phosphate cement with recombinant human vascular endothelial growth factor and bone morphogenetic protein-2 in repairing osteo-articular defect.Methods:36 adult mixtus dogs were constructed as semi-articular defect models through resecting 30mm right femur inferior extremity near articular facet,and they were randomly divided into 3 groups.In group A,material CPC/rh VEGF/rh BMP-2/DBP was transplanted into the semi-articular defect.In group B,material rhVEGF/rhBMP-2/DBP was transplanted there.In group C,simple material DPB was transplanted there.At 4,8,12 and 16 weeks after operation,the effects were as- sessed by macro-morphology observation,X-ray examination,histologic examination as well as collagen type I immunohistochem- istry analysis transmission electron microscope examination and vitodynamic tests in order to observe bone reparation matter. Results:The new bone formation observed by vitodynamic tests,macro-morphology observation,X-ray examination,histologic ex- amination and transmission electron microscope examination in group A was significantly superior to that in group B and C.The results of collagen type I immunohistochemistry analysis and vitodynamic tests were significantly superior to that in group B and C(P0.05).Conclusion:The mate- rials CPC/rh VEGF/rh BMP-2 can promote the new bone formation,achieve bone healing and make them become autologous tissue at last.

OBJECTIVE:To develop a RP-HPLC method for the simultaneous determination of Notoginsenoside R1 and Ginsenoside Rg1 in Danqi tablets. METHODS:The chromatographic separation was performed on a Kromasil C18(250mm?4.6mm,5?m) column with column temperature at room temperature. The mobile phase consisted of acetonitrile-water-H3PO4(20.5∶79.5∶0.02) at a flow rate of 1.0mL?min-1.The detection wavelength was set at 203nm. RESULTS:The linear range of Notoginsenoside R1 and Ginsenoside Rg1 were 4.67～46.68?g?mL-1 (r=0.999 0) and 4.62～115.50?g?mL-1 (r=0.999 9), respectively; with the average recover of Notoginsenoside R1 at 97.93% and that of Ginsenoside Rg1 at 97.98%, and RSD at 1.31%(n=6) and 1.38%(n=6), respectively. CONCLUSION:The method is simple, rapid and accurate, and suitable for the quality control of Danqi tablets.

Objective To summarize the characteristics of diagnosis and surgical treatment of the leiomyoma of cardia.Methods The clinical data of 18 cases of leiomyoma of cardia confirmed by pathological examination was retrospectively analysed.Results Dysphagia,chest or epigastric pain and gastric hemorrhage were the most common presenting symptoms.The average course of disease was 9 months.Two patients was misdiagnosed as carcinoma of cardia before operation.Seventeen patients received partial gastric resection,one patient proximal subtotal gastrectomy.No surgical death and severe complications occurred in all patients.Conclusion Leiomyoma of cardia is a rare benign tumor.The differential diagnosis is important because it may be misdiagnosed as malignancy.Surgery is the most effective therapy to this disease.

ObjectiveTo evaluate the clinical effect of the percutaneous fixation for atlas-axis combined fractures in the elderly patients,especially with brain injury.MethodsFrom March 2006 to February 2011,a total of 7 cases with C1,2 combined fractures entered the study,including 5 males and 1 female with an average age of 72.4 years (range,64-84 years).Fracture combinations included two Jefferson/type Ⅱ odontoid,two anterior ring/type Ⅱ odontoid,two anterior and posterior ring/type Ⅱ odontoid,and one anterior and posterior ring+lateral mass/type Ⅲ odontoid.There were 5 patients with head injuries in varying degrees of coma.All patients underwent percutaneous placement of odontoid screw and anterior C1,2 transarticular screws (triple anterior screw fixation),and were immobilized in a hard collar for 3 months postoperatively.Results The average duration of the operation was 56 min(range,36-78 min).Satisfactory results were achieved in all patients,and all screws were in good position.There were no intraoperative surgery-related complications.The mean follow-up period was 10.7 months.During the follow-up,no pullout,loosening,distortion,or breakage of internal screws was observed.Radiographic union occurred in all odontoid fractures and C1,2 stable was found in all case.ConclusionFor elderly patients especially those with brain injury,the triple anterior screw fixation is reliable and effective in dealing with combined atlas-axis fractures.This percutaneous surgery has the advantages of less invasive,less blood loss,less pain and early recovery.

ObjectiveTo investigate the differences and the similarities of clinical presentations and CT features between type B3 thymoma ( atypical thymoma) and type C thymoma ( thymic carcinoma) in the WHO classification of thymic epithelial tumors.MethodsComplete CT findings of thirty cases of type B3 and seventeen cases of type C thymic epithelial tumors confirmed by histopathology according to WHO 2004 Classification System and clinical features including the prognosis of each case were reviewed retrospectively.Statistical analyses of the data for the age and the long diameter were performed with Independent-Samples t test between the two groups.Statistical analysis for gender,association with myasthenia gravis,method of the operation,contours,shapes,calcification,necrosis,enhancement pattern of the tumors on CT,presence of mediastinal lymphadenopathy,invasion of mediastinal fat,chest wall,pericardium,great vessel,pleural mediastinum,metastasis to the plural,pleural effusion,distant metastasis were performed with Fisher exact test.Kaplan-Meier method was employed for survival analysis.Results Clinical data:the average age of type B3 group was significantly younger ( t = - 2.905,P = 0.006).90.0％(27/30) of patients in type B3 group were complicated by myasthenia gravis,while only 5.9％ (1/17) of patients in type C group were complicated by myasthenia gravis.The difference between the two groups was statistically significant ( P = 0.000 ).The ratio of complete resection of type B3 group ( 80.0％ ) was significantly higher than that in type C group ( P = 0.001 ),70.6％ (12/17) of patients in type C group died within 2 years after surgical resection,while only 20.0％ (6/30) of patients in type B3 group died within one to nine years after surgical resection.Three years'survival ratio of C group was 29.4％,and five years'survival ratio of C group was lower than 14.7％,which was significantly lower than B3 group( five years'survival ratio was 94.7％ ),which was statistically significant (P =0.000).CT features:the average long diameter of tumor in type C group was ( 6.4 ± 2.1 ) cm,significantly longer than that in type B3 group,which was (4.6 ± 2.3) cm (t = - 2.778,P = 0.008 ),and the contour of tumor in type C group usually showed irregularity ( 13/17,76.5％ ),while it usually showed lobulated ( 18/30,60.0％ ) in type B3 group (P = 0.019 ),more necrosis found in C group ( 15/17,88.2％ ) than B3 group ( 17/30,56.7％ ),compared with type B3 group on enhanced CT,more tumor in type C group showed heterogeneous enhancement (P =0.042),and more great vessel invasion ( 15/17,88.2％,P =0.001 ) and more distant metastasis (8/17,47.0％,P = 0.028) occurred in type C group.ConclusionMost of type B3 and type C thymoma have a certain significant difference in clinical factor,prognosis and CT findings,which are helpful for differentiation.

TLC scanning technique was, developed for the determination of matrine in Jieshenbao lotion. The method is simple, and highly sensitive, with an average recovery of 99.87%and a variation cofficient of 1.33%.

Carcinoid tumors are relatively rare neuroendocrine malignancies most commonly originating from enterochromaffin cells in the gastrointestinal tract.Carcinoid syndrome occurs when tumor cells metastasize to the liver,as the vasoactive substances produced are able to reach the systemic circulation via the hepatic vein.The development of carcinoid heart disease (CHD) is thought to be related to the vasoactive substances secreted by the metastatic tumor cells in the liver,reaching the right heart.This is associated with deposition of fibrous tissue on the endocardial surfaces of the heart.Several series have reported CHD in up to 70% of cases of carcinoid syndrome.Somatostatin analogue use is a therapeutic cornerstone,effective in symptomatic control of the endocrine syndrome.In patients with severe cardiac involvement and well-controlled systemic disease,valve replacement surgery is an effective treatment modality that can relieve intractable symptoms and contribute to improved outcome.

Objective: To evaluate the effects of one lung ventilation on hemodynamics and blood gas during thoracoscopic surgery in piglets. Methods: Eight domestic pigs weighting (7.1±0.7) kg were included in this study. The anaesthesia was maintained with 1% isoflurance. After tracheotomy, an endotracheal tube was positioned in the left mainstem bronchus. Hemodynamic parameters as well as blood gas analyses were obtained using Swan-Ganz and arterial catheter. Results: During one lung ventilation, pulmonary artery pressure increased from (15.8±1.5) mm?Hg to (19.5±2.3) mm?Hg. Arterial oxygen saturation, blood gas analyses and other hemodynamic parameters remained unchanged. Conclusion: One lung ventilation was well tolerated in regard to hemodynamics and blood gas analyses in piglets.

AIM: To establish a HPLC method for determining of ginsenoside Re,ginsenoside Rg_1 and schisandrin in Huoliyuan Tablets(total ginsenoside,Radix Astragali,Fructus Schisandrae Chinensis,Radix Ophiopogonis,etc.). METHODS: A Hypersil C_(18) column was used.The mobile phase for ginsenoside Re and Rg_1 was acetonitrile-water mixed with 0.05 mol/L NaH_2PO_4(pH2.7,20∶80),and determination wavelength was 203 nm.The mobile phase for schisandrin was acetonitrile-0.1%H_3PO_4(48∶52),and determination wavelength was 250 nm. RESULTS: The linear ranges of ginsenoside Re,Rg_1 and schisandrin were 1.84-14.72 ?g(r=0.999 4),1.49-11.92 ?g(r=0.999 0) and 0.094-0.47 ?g(r=0.999 9),respectively.The average recoveries of ginsenoside Re,Rg_1 and schisandrin were 99.86%(RSD=1.69%),98.17%(RSD=2.03%) and 101.54%(RSD=(2.62%),) respectively. CONCLUSION: The method is simple,accurate,and can be used for the quality control of Huoliyuan Tablets.