Objective:To explore the nursing knowledge of extracorporeal membrane oxygenation (ECMO) in cardiac surgery nurses and analyze its influencing factors, in order to provide a reference for improving the nursing ability of clinical cardiac surgery nurses.Methods:Totally 104 nurses were selected from the Department of Cardiac Surgery, the First Affiliated Hospital of Zhengzhou University from January 2017 to January 2018, and investigated with the self-designed questionnaire of ECMO-related nursing knowledge and attitude. The status quo and influencing factors of ECMO nursing knowledge in cardiac surgery nurses were analyzed.Results:For the content of ECMO-related nursing knowledge, the percentages of nurses giving the 100% correct answers to "bleeding, anticoagulation problems and nursing measures", "nursing measures of the circulatory system", and "management of the catheter" were higher, being 92.31%, 88.46%, and 84.61% respectively, while the dimension to which the percentage of nurses giving 100% correct answers was the lowest was "prevention and control of related nosocomial infections", which was only 65.38%. The total proportion of nurses who gave 100% correct answers was 79.81%. There was no statistically significant difference in ECMO-related nursing knowledge scores between nurses at different ages ( P>0.05) . The difference in ECMO-related nursing knowledge and attitude scores between nurses with different working years, positions, and educational backgrounds was statistically significant ( F/t=96.834, 12.014, 44.726; P<0.05) . Multiple linear regression analysis showed that 1-3 years of working and educational backgrounds were the influencing factors to the difference in ECMO nursing knowledge scores between cardiac surgery nurses ( P<0.05) , and two variables could account for 65.7% of the variance of ECMO-related nursing knowledge scores in cardiac surgery nurses ( R2=0.657, P<0.01) . Conclusions:Cardiac surgery nurses have a good grasp of ECMO-related nursing knowledge, and 1-3 years of working and educational backgrounds were the influencing factors its scores. Cardiac surgery nurses still need to strengthen learning and training in terms of aseptic technical operation practices and related prevention measures for hospital infection.