Objective To investigate the application and clinical significance of flow cytometry(FCM)in cerebrospinal fluid(CSF)detection in the diagnosis of central nervous system leukemia(CNSL).Methods By the CD34,CD45 based different monoclonal antibody combined FCM detection method,CSF of 100 patients with CNSL was detected multi parameter of minimal residual disease(MRD).And with the natural sedimentation of cerebrospinal fluid after smear method,CSF cytology results were compared and analyzed.Results Of the 100 patients,CSF exami-nation was positive in 42 patients.CSF cytology was positive in 16 cases,the difference was statistically significant (χ2 =4.19,P <0.05).10 cases of nervous system symptoms of CSF examination were positive,and only 5 cases of CSF cytology positive(χ2 =6.81,P =0.018).In 100 cases of MRD detection,18 cases increased in white blood cell count,FMC detected in 17 cases,cytological diagnosis of 11 cases (χ2 =5.89,P =0.024).CSF with elevated pres-sure in 11 patients,FCMpositive in 11 cases,cytology detected in 4 cases(χ2 =5.12,P =0.031).CSF protein was elevated in 19 cases,FCMdetected 17 cases,cytology detected 7 cases(χ2 =7.14,P =0.001).Conclusion FCM combined with routine CSF detection can improve the positive rate of CNSL diagnosis,and it has important significance for early detection and treatment of CNSL,and to prolong the disease -free survival of patients with leukemia.

Objective To investigate the clinical significance of flow cytometry(FCM)in cell apoptosis and prognosis in patients with acute leukemia.Methods FCMpropidium iodide(PI)DNA quantitative stain was used in 55 patients with acute leukemia(acute lymphoblastic leukemia treatment in 20 cases,alleviating period of 25 cases, 10 cases of acute non lymphocytic leukemia)to detect cell apoptosis and DNA cycle content changes,and compared with the control group.Results Acute leukemia cell apoptosis percentage Apo% in remission period was the highest, and it was (26.70 ±4.11)%,which of the initial treatment group was (10.23 ±1.05)%,remission with early treat-ment group and control group,the differences were statistically significant(t =3.970,2.341,all P <0.05).Leukemia DNA index (DI),aneuploid (AN rate),S phase cell percentage (SPF)value were (2.03 ±0.66 ),40% and (15.68 ±2.17)respectively,which were significantly increased.Conclusion The content of DNA in patients with acute leukemia is abnormal,the amount of apoptosis,SPF and other indicators have certain reference value for the prognosis of patients with acute leukemia.

Objective To study the changes of tartrate resistant acid phosphatase in the serum of patients with bone metastasis in pros-tate cancer,and to explore its clinical significance for bone metastasis diagnosis and prediction of prostate cancer. Methods Seventy-eight cases of prostate cancer and 40 cases of benign prostatic hyperplasia patients as the research object,at the same time,40 healthy young men as controls,were divided into the group of patients with prostate cancer bone metastases (group A,n=41),prostate cancer patients with no bone metastasis group (group B,n=37),benign prostatic hyperplasia patient group (group C,n=40),healthy control group (group D,n=40). Determination of serum TrACP levels in patients with the immunoassay using double antibody sandwich ELISA,combined with pathologi-cal grade,Gleason score,PSA,ALP and ALT were statistically analyzed. Results The serum TrACP concentration in patients with bone me-tastasis significantly increased,with significant difference compared with the other groups. The concentration of serum TrACP and PSA levels showed a positive correlation. The area under the ROC curve ( AUC) was higher than that in ALT,and the ROC curve of cross TrACP and PSA,suggesting that high value in the diagnosis of bone metastasis. Conclusion The detection of TrACP has directly diagnosis and predic-tive value for prostate cancer with bone metastasis,the serum TrACP content monitoring in patients with prostate cancer has valuable clinical significance in understanding the growth progression status of prostate cancer,judgment and prediction of bone metastases.

Purpose:To study the reversal effects on multidrug resistance in multidrug-resistent bladder cell lines BIU-87/ADM. Methods:The degree of drug-resistence was detected by MTTmethod; the concentration of ADM in cells BIU-87 and BIU-87/ADM was detected after the cells were treated by carvedilol and ADM. Results:Pretreatment by carvedilol increased the concentration of ADM in BIU-87/ADM cells, ,and the concentration was much higher than that in those cells treated by ADM only. Conclusions:Carvedilol can enhance the cytotoxic effect of ADM,and can reverse the multidrug resistance in bladder cancer lines BIU-87/ADM.

Objective:To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell.Methods:Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8.The invasion of HepG2 was detected by Transwell assay.The expression of MMP-2 and MMP-9 was analyzed by ELISA.Results:In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure.In the Transwell assay,the inhibitory rate of invasion was 52.45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2.65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang(P<0.05). Conclusion:Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.

Objective:To investigate the surgical treatment for compression of inferior vena cava (IVC) by hepatic hydatid cyst.Methods:The retrospective and descriptive study was conducted. The clinical data of 10 patients with hepatic hydatid cyst compressing inferior vena cava who were admitted to the First Affiliated Hospital of School of Medicine of Shihezi University from May 2013 to May 2018 were collected. There were 7 males and 3 females, aged (46±5)years, with a range from 38 to 51 years. Patients underwent subadventitial close total pericystectomy, subadven-titial partial pericystectomy or subadventitial close total pericystectomy with partial IVC resection according to the relative distance between hepatic hydatid cyst and IVC, and the feasibility of total pericystectomy. Observation indicators: (1) surgical situations; (2) postoperative situations; (3) follow-up. Follow-up was conducted using outpatient examination or telephone interview. Patients were followed up at the outpatient department at postoperative 3, 6, 12, 24 months using color Doppler ultrasound examination to detect the recurrence of hepatic hydatid cyst and inferior vena cava occlusion. The endpoint of follow-up was 2 years after operation, and the secondary endpoint of follow-up was recurrence of hepatic hydatid cyst. The follow-up time was up to May 2020. Measure-ment data with normal distribution were represent as Mean±SD, and count data were described as absolute numbers. Results:(1) Surgical situations: 10 patients underwent surgery successfully without massive hemorrhage and septic shock during the operation, including 7 patients undergoing subadventitial close total pericystectomy, 2 patients undergoing subadventitial partial pericys-tectomy, and 1 patient undergoing subadventitial close total pericystectomy with partial IVC resection. The operation time and volume of intraoperative blood loss of 10 patients were (99±27)minutes and (99±48)mL, respectively. (2) Postoperative situations: the time to drainage tube removal and duration of postoperative hospital stay of 10 patients were 2-5 days and (7.1±1.8)days. None of patients underwent postoperative bleeding, residual cavity infection, biliary fistula, or perioperative death. (3) Follow-up: of 10 patients, 9 patients completed the endpoint of follow-up and no recurrence of hepatic hydatid cyst or inferior vena cava occlusion formation was detected. The other 1 patient was followed up at postoperative 3, 6 months but failed to be followed up at postoperative 12 months, and no recurrence of hepatic hydatid cyst or inferior vena cava occlusion formation was detected during the follow-up time.Conclusion:Complete resection of hepatic hydatid cyst can be achieved by subadventitial close total pericystectomy, subadventitial partial pericystectomy or subadventitial close total pericystectomy with partial IVC resection.

AIM:To analyze the changes in the active components alignment of Puerariae Decoctions between its two different compatibility proportion. METHODS: Reversed phase high-performance liquid chromatography (RP-HPLC) was applied. RESULTS: When the compatibility ratio of Radix Puerariae Lobatea, Radix Scutellariae, Rhizoma Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum melle was 5∶3∶3∶2, the contents of the eight drug active components alignment were 2.48% puerarin, 0.101% daidzin, 0.614% baicalin, 0.155% berberine, 0.048% palmatine, 0.094% jateorhizine, 0.154% glycyrrhizic acid, 0.283% liquiritin, and the proportional relationship of compatibility was 51.7∶2.1∶12.8∶3.2∶1∶2∶3.2∶5.9. When the compatibility ratio of Radix puerariae lobatae, Radix scutellariae, Radix Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum melle was 8∶3∶3∶2, the proportion of contents of the eight active components alignment were 1.67%, 0.143%, 0.36%, 0.085%, 0.031%, 0.048%, 0.126%, 0.174%. And the proportional relationship of compatibility was 54.4∶4.7∶11.7∶2.6∶1∶1.5∶4.1∶5.7. CONCLUSION: When the compatibility percentage consists of 8∶3∶3∶2, only the puerarin content relatively increases. The active components alignment of the rest have no significant difference. This illustrates the contents of the active components alignment of puerariae decoction is related to the compatibility percentage and herb solubility.

OBJECTIVE To explore the factors and effective preventive method of nosocomial lower respiratory(infection) after general anesthesia through trachea intubation.METHODS Using bacterial culture and identification technique to detect the samples collected from pipe and the sides of anesthetic machine,from disposable virus/(bacteri)a respiratory filter and buccal and bronchial secretion of patients with general anesthesia through trachea(intubation.) RESULTS Eight of 15 anesthetic machines without disinfection were with positive bacterial culture.All sides of virus/bacteria respiratory filter,and buccal and bronchial secretion of patients during operation had same(bacteria.) No bacteria growth was found in anesthetic machine and disposable virus/bacteria respiratory filter used after operation.The same bacteria as from buccal secretion were isolated from anesthetic machine in patients(without) using virus/bacteris respiratory filter.CONCLUSIONS The(results) showed the anesthetic machine is more easy to be contaminated by patients,and the reuse of anesthetic machine is an important factor for nosocomial(lower) respiratory infection after general anesthesia through trachea intubation.Intensifying periodic disinfection of(anesthetic) machine and using virus/bacteria respiratory filter can prevent contamination between anesthetic(machine) and patients and decrease nosocomial lower respiratory(infection) after operation.

Purpose:To study the expression of TopoⅡ gene in multidrug resistant cells of human bladder cancer. Methods:The degree of drug resistance was detected by MTT method ;the expression of TopoⅡ gene in cell lines BIU 87/ADM and BIU 87 was detected with reverse transcriptase polymerase chain reaction. Results:The cell lines BIU 87/ADM were 56.4 times more resistant to ADM than the cell lines BIU 87;the expression of TopoⅡ gene was poorly positive in BIU 87/ADM but strongly positive in BIU 87 cells. Conclusions: The decreased expression of TopoⅡ gene in BIU 87/ADM cells might contribute to the development of multidrug resistance of human bladder cancer.

Objective To predict the B cell epitope for MUC1 antigen. Methods The secondary structure and surface properties of MUC1, such as physical and chemical characters, hydrophilicity, antigenicity, were analyzed with various methods. Results Many distinct antigenic epitopes in MUC1 were identified by computation: 1-10, 24-54, 65-77, 84-91, 108-134, 140-156, 159-174, 179-196, 199-210, 220-233, 237-265, 270-299, 316-337, 351-362, 369-396, 411-420, and 445-502. Conclusion Prediction of the B cell epitope for MUC1 can provide a base for the studies of structure and function of MUC1, construction of its mutants, and selection of new expression forms of MUC1.