Objective ::To investigate the role of trehalose in hepatic ischemia-reperfusion injury and its underlying mechanisms.Methods:C57BL/6J mice were randomly divided into no-ischemia group, ischemia-reperfusion group, trehalose-treated group and normal saline control group. After ischemia for 90 minutes, reperfusion immediately or 6h, blood and liver tissues were collected, and serum was separated. The liver function parameters of ALT, AST, the inflammatory factors of TNF-α, IL-1β and IL-2, and the pathological changes of liver were detected to study the role of trehalose during hepatic ischemia-reperfusion injury. Hypoxia-reoxygenation cell model was established by AML12 mouse hepatocyte line, and divided into experimental group and control group. The experimental group was divided into low dose group and high dose group according to the concentration of trehalose administrated. And the control group had no use of trehalose. The level of apoptosis was measured to study the effect of trehalose on apoptosis induced by hepatic ischemia-reperfusion injury with flow cytometry. Western blot was utilized for detecting the levels of Caspase-3, Cleaved Caspase-3 and Bcl-2 protein to understand the molecular mechanisms of trehalose in apoptosis during hepatic ischemia-reperfusion injury.Results:In vivo animal experiments showed that liver function and such inflammatory factors as ALT, AST, TNF-α, IL-1β and IL-2 increased in ischemia-reperfusion group after hepatic ischemia-reperfusion ( P<0.05), and liver tissue became necrotic. After a treatment of trehalose, the levels of ALT, AST, TNF-α, IL-1β and IL-2 were lower than those of normalsaline control group and the area of liver tissue necrosis also decreased ( P<0.05). In vitro cell experiments showed that the apoptosis level of hepatocytes in the experimental group decreased compared with the control group.And the level of activated pro-apoptotic protein Cleaved Caspase-3 decreased, the level of anti-apoptotic protein Bcl-2 increased. Conclusions:Trehalose has protective effects on hepatic ischemia-reperfusion injury in vivo and in vitro. The mechanism may be involved in inhibiting inflammation induced by hepatic ischemia-reperfusion injury, suppressing the activation of Caspase-3 and promoting the expression of Bcl-2, thus played a protective role by extenuation of hepatocyteapoptosis.

Objective To explore the application effect of nosting barrier film in the skin nursing of laryngeal carcinoma patients with trachea cannula after surgery.MethodsTotally 90 cases of laryngeal cancer patients after radiotherapy with tracheal cannula,were randomly divided into three groups,with 28 cases in the control group,33 cases in the dressing group,and 29 cases in the skin protective film group. Patients in the control group were treated with basic nursing method including using saline to clean plastic casing,cleaning and disinfecting skin,using sterile gauze to cover the field of radiation skin and plaster fixation;dressing group adopted Comfeel hydrocolloid dressings on the basis of basic nursing method;skin protective film group adopted Genetime spray skin protective film to protect skin of radiation field on the basis of basic nursing. After radiotherapy,the incidence of skin injury,severity of skin injury,patient comfort,and the time and times of changing skin dressing everyday were observed and compared among three groups.Results The incidence of radiation skin injury in the skin protective film group (6.89%) and dressing group (15.15%),were all lower than the control group (50%) (χ2=16.75,P<0.05);the times of changing skin dressing everyday in the skin protective film group (4.28±0.53) times/d and the dressing group (4.52±0.76) times/d,were lower than the control group (6.04±1.35) times/d (Hc=32.65,P<0.05);patient comfort in the skin protective film group and the dressing group was superior to the control group (Hc=22.42,P<0.05);the time of changing skin dressing in the skin protective film group (10.40±1.35) min,was less than the dressing group (14.09±1.27) and the control group (12.35±1.28) min (F=85.87,P<0.05).Conclusions The skin protective film can effectively reduce the incidence of skin injury in patients with laryngeal cancer and radiotherapy,improve patient comfort,reduce the labor time of nurses,and improve the quality of care and efficiency.

Objective To explore the value of pediatric end-stage liver disease (PELD)score system in predicting prognosis after pediatric living donor liver transplantation (LDLT). Methods Clinical data of 101 infants undergoing living-donor liver transplantation from October 2006 to December 2012 in Department of Liver Surgery in Affiliated Renji Hospital of School of Medicine of Shanghai Jiaotong University,were analyzed retrospectively. All infants were diagnosed as biliary atresia. PELD scores before LDLT were graded. According to PELD scores,all the patients were divided into two groups:low score group (PELD score <16,n=62) and high score group (PELD≥16,n =39 ).The basic data during perioperative period and incidence of postoperative complications were compared between two groups. Results There were significant differences in age and body weight between two groups (both in P<0.05 ). But there was no significant difference between the two groups in gender,graft to recipient weight ratio (GRWR),cold isehemia time and intraoperative blood loss (all in P>0.05 ). The incidence of lung infection and biliary complications in high score group were significantly higher than those in low score group. Conclusions Preoperative PELD score can be used in predicting prognosis after pediatric LDLT and provide a reference for the treatment,caring and nursing during perioperative period of pediatric LDLT. For infants with high PELD score before operation,the care of perioperative complications should be enhanced.