Objective To explore the effect of long non-coding RNA CASC11(lncRNA CASC11)on the proliferation and invasion of colon cancer cells by targeting and regulating miR-146a-3p and its possible mechanism.Methods The expressions of lncRNA CASC11 and miR-146a-3p in three different human colon cancer cell lines HCT116,SW620 and SW480 were detected by real-time fluorescence quantitative PCR(qRT-PCR),and SW620 cells cultured in vitro were randomly divided into control group(untreated group),lncRNA CASC11 siRNA group,lncRNA CASC11 siRNA negative control group and lncRNA CASC11 siRNA+miR-146a-3p inhibitor co-transfection group.After corresponding treatment,the proliferation activity of SW620 cells in each group was detected by MTT and EdU staining;the invasion ability of SW620 cells was detected by Transwell chamber test;immunofluorescence staining(ICF)was used to detected the expression of apoptosis proteins Bax and Bcl-2 in each group;mRNA levels of miR-146a-3p and epithelial-mesenchymal transition marker proteins Vimentin and E-cadherin were detected by qRT-PCR and Western blotting;dual luciferase reporter assay was used to detect the targeting regulation effect of lncRNA CASC11 on miR-146a-3p in SW620 cells.Results Compared with human colonic mucosal epithelial cells,the expressions of ln-cRNA CASC11 in HCT116,SW480,and SW620 were significantly increased,while the expression of miR-146a-3p was signifi-cantly decreased(P＜0.05).Compared with the control group,the cell viability,EdU positive rate,invasive cell number and Vi-mentin mRNA expression in the lncRNA CASC11 siRNA group were decreased(P＜0.05),while the cell Bax/Bcl-2 ratio,miR-146a-3p and E-cadherin mRNA expression were increased(P＜0.05).There was no significant difference in the results between the control group and lncRNA CASC11 siRNA negative control group(P＞0.05);compared with the lncRNA CASC11 siRNA group,the cell viability and EdU positive rate,the number of invasive cells,and the expression of Vimentin mRNA were in-creased in co-transfection group(P＜0.05),while the ratio of Bax/Bcl-2,miR-146a-3p and E-cadherin mRNA expression were decreased(P＜0.05).In SW620 cells,lncRNA CASC11 could target miR-146a-3p and upregulate the expression of downstream c-Met gene.Conclusion lncRNA CASC11 mediates the biological behavior of colon cancer cells by targeting downregulation of miR-146a-3p.Knockdown of lncRNA CASC11 can promote the expression of miR-146a-3p and inhibit the proliferation and in-vasion of colon cancer cells.

Objective:To compare the clinical outcomes between modified percutaneous kyphoplasty (PKP) and conventional PKP in the treatment of Kümmell disease.Methods:A retrospective cohort study was conducted of the 57 patients with single level kummell disease who had undergone PKP between January 2015 and December 2017 at Department of Spinal Surgery, Jiangmen Hospital Affiliated to Sun Yat-sen Uni-versity.Of them, 36 received modified PKP and 21 conventional PKP.In the modified PKP group, there were 24 males and 12 females with an age of 73.9 years±9.4 years and a course of disease of 2.2 months±1.5 months; the disease was located at a thorocic vertebra in 20 cases and at a lumbar vertebra in 16.In the conventional PKP group, there were 15 males and 6 females with an age of 72.6 years±11.9 years and a course of disease of 1.8 months±1.1 months; the disease was located at a thorocic vertebra in 10 cases and at a lumbar vertebra in 11.The 2 groups were compared in terms of operation time, bone cement volume, cement leakage, visual analogue scale (VAS), Oswestry disability index (ODI), anterior and middle heights and cobb angle of injured vertebra at postoperative 3 days, 1 and 3 months, and final follow-up.Results:There were no significant differences in the general clinical data between the 2 groups, showing compatibility between groups ( P>0.05).All the operations were accomplished uneventfully with no such serious complications as spinal cord nerve injury, wound infection, pulmonary embolism, bone cement poisoning reaction or death. There was no significant difference between the 2 groups in operation time or bone cement volume ( P>0.05).The rate of cement leakage in the modified PKP group [33.3%(9/27)] was significantly lower than that [52.4%(11/21)] in the conventional PKP group ( P<0.05).There were no significant differences be-tween the 2 groups in VAS, ODI, anterior or middle height of injured vertebra or cobb angle at postoperative 3 days or one month ( P>0.05), but the postoperative values were improved compared to the preoperative ones.At postoperative 3 months and final follow-up, the modified PKP group was significantly better in all the indexes than the conventional PKP group ( P<0.05). Conclusion:Compared with conventional PKP, the modified PKP may lead to better outcomes for Kümmell disease.