Objective To study the mechanism of protein extracted from nidus vespae protein(NVP) on anti-leukemia and provide experimental and theoretical evidence for exploring new anti-leukemia drugs. Methods The effect of the NVP on cell growth and morphological changes of K562 cells was observed by using cell culture and transmission electronic microscopy,the effect of NVP on apoptosis-associated signal transduct protein NF-? Bp65,?-catenin and iNOS of K562 cells was detected via immunohistochemical method and image analysis system. Results(1) The saturation destinity of K562 cells in NVP group was decreased which suggested that the proliferation was inhibited.(2) In the NVP group typical apoptosis morphological changes were observed in the K562 cells under transmission electronic microscope.(3) The result of immunohistochemistry showed that the expression of NF-? Bp65,?-catenin and iNOS of K562 cells in the NVP group decreased.The optical density(A) values of NF-?Bp65,?-catenin analysed by the image analysis system were decreased in the NVP group.Conclusion The NVP can inhibit the proliferation of K562 cell prominently,and the mechanism probably is that the apoptosis is induced via regulating the expression of apoptosis-associated signal transducted factors NF-?Bp65,?-catenin and iNOS.

Abstract China has one of the world’s highest prevalence of myopia in children and adolescents, and children are developing myopia earlier. Myopia has become a important issues affecting children and adolescents health, as well as a significant public health concern. There has been continuing debate over the role of nature and nurture in the aetiology of myopia, growing evidence showed that environmental factors has a determine effect on myopia.The randomized controlled trials has assured the relationship between time outdoors and myopia. Based on reviewed published articles, survey and intervention studies and school health practices, according to the principle of school organization, student participation and family cooperation, the authors proposed outdoors activities as initiative for myopia prevention and control in children and adolescents. The detail recommendation contents, levels were list out.

Diabetes mellitus with ketoacidosis and combined with coma are acute critical complications, which can be complicated with acute abdomen, such as acute pancreatitis, mesenteric thrombosis, small intestine necrosis, etc. There is no report of massive intestine necrosis in the previous literature. We present an overview and aim to improve the diagnosis of acute complications in diabetes mellitus combined with acute abdomen.