Objective To study the location and the expression of nerve growth factor(NGF)in mouse testes at different developmental stages.Methods The testes were taken out from mice at different developmental stages,respectively.Parts of the testis tissue were used to extract total RNA and real-time PCR was used to detect the expression of NGF.The rest tissues were fixed and embedded in paraffin.SABC immunohistochemical staining was used to observe the location of NGF in the testes. Results Reat-time PCR results showed that NGF mRNA could be detected in the testes of mouse at postnatal 1 st week and its expression level increased with the development,and reached the highest point at 3rd week.After 5 weeks it lowered down to a stable level.Immunohistochemistry showed that NGF expressed in the mouse testes at all developmental stages.At postnatal 1 st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining.NGF positive staining in the testes was observed in interstitial cells,spermatogenetic cells,sustentacular cells and Leydig cells at 3rd week.After the postnatal 5th week,NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells,but the intensity of reaction was weaker than that at 1 st and 3rd weeks.Conclusion The expression levels of NGF in mouse testes show regular changes and the expression profile and location of NGF in the testes are different at different developmental stages.

Objective To explore the apphcation of the integrated teaching method of PBL and TBL based on cases in pharmacology teaching.Method Ninety-two students majored in Pharmaceutical Preparations were selected into the control group,and 184 students majored in pharmacy were enrolled into the experimental group.The teaching effect was comprehensively evaluated by using the combination of theoretical examination and student feedback.Results In the experimental group,the average score of students in Pharmacology (73.68 ± 9.40) was significantly higher than that of the control group (67.34 ± 7.18),and the feedback of the students in the experimental group was significantly better than that in the control group.Conclusion Compared with the traditional teaching method,the integrated teaching method can significantly improve the students' theoretical examination results and students' learning interest.

Objective To investigate MRI characteristics of subacute combined degeneration(SCD)with secondary spinal canal stenosis.Methods The clinical and MRI imaging data of 56 patients with SCD were collected to analyze the performance characteristics between spinal cord lesions and spinal canal stenosis,which depended on the synergism of lumbar disc bluge or herniation,degenerative thickening of the ligament flavum and posterior longitudinal ligament.Results Among 56 SCD cases underwent MRI scan,45 cases were combined with spinal cord lesions which showed typical signs of SCD.37 patients were secondary spinal canal stenosis with typical signs,but 2 showed no typical signs.8 patients were no secondary spinal canal stenosis and showed typical.9 cases showed neither spinal cord lesions nor secondary spinal canal stenosis.There was significant difference (P <0.05)between relative secondary spinal canal stenosis and spinal anomaly signal.The course of 1 5 cases were shortened after treated by physical in 37 cases of SCD with secondary spinal canal. Conclusion The secondary spinal canal stenosis can cause microcirculation dysfunction of the spinal cord,which is a key factor contributing to the imaging manifestation.

Diabetic retinopathy (DR) is one of the most common causes of visual impairment and blindness in diabetic patients.It is particularly important to set up simpler,safer,non-invasive and highly effective methods for diagnosis as well as monitoring DR.A variety of new fundus imaging techniques show great advantages in early diagnosis,treatment and monitoring of DR in recent years,The main characteristics of wide-field scanning laser imaging system is achieving a large range of retinal image in a single photograph and without mydriasis.It provides several options for color images,FFA and FAF,which satisfy to detect the retina,choroid and vascular structure.Multi spectral fundus imaging system is suitable for DR screening,because it is able to recognize the typical characteristics of DR,such as microaneurysms,hemorrhage and exudation,and is non-invasive and convenient.OCT angiography is a quantitative examination that provides foveal avascular zone area,macular blood flow density,which provides strong evidence for DR diagnosis.The improvement of these new techniques will help us to build up a personalized evaluation system of DR.

Objective:To summarize the methods and effects of utilizing the ilioinguinal flap in repairing the defect after the extended resection of the middle and lower abdominal wall or the groin region’s dermatofibrosarcoma protuberans.Methods:From November 2011 to October 2018, nine patients (including five males) with the middle and lower abdominal wall or groin region’s dermatofibrosarcoma protuberans received extended resection procedures in the Department of Plastic and Reconstructive Surgery of Sun Yat-sen Memorial Hospital, Sun Yat-sen University. The age distribution of patients was 27-60 years old with a mean age of 44.3 years old. The defect area following the extended resection of the tumor ranged from 9.5 cm × 10.5 cm to 15.0 cm × 18.0 cm, and the size of the ilioinguinal flap ranged from 6.0 cm × 11.0 cm to 8.0 cm × 15.0 cm. Either an axial flap or a V-Y advancement flap was used to repair the defect. Five patients were treated with the unilateral ilioinguinal flap, two with the bilateral ilioinguinal flap combination flap, one with the ilioinguinal flap combined with the tensor fascia lata myocutaneous flap, and one with the ilioinguinal flap combined with the anterolateral thigh flap.Results:All defects were covered by flaps completely, and all donor sites were closed directly. Eight flaps survived completely without necrosis, while one case of ilioinguinal flap faced the distal necrosis, which was debrided and repaired with full-thickness skin grafting in the second stage. The postoperative follow-up period ranged from 1.0 to 8.5 years. No local recurrence or other distant metastases occurred. All reconstructed regions had a good outline and ordinary sense function.Conclusions:Almost all defects after the extended resection of dermatofibrosarcoma protuberans in the middle and lower abdominal wall or the groin region could be repaired with a unilateral or bilateral ilioinguinal flap, whose blood supply is stable and reliable. Moreover, the operation presented is simple, less time-consuming, safe, reliable, and with a satisfactory postoperative appearance.

Objective:To summarize the methods and effects of utilizing the ilioinguinal flap in repairing the defect after the extended resection of the middle and lower abdominal wall or the groin region’s dermatofibrosarcoma protuberans.Methods:From November 2011 to October 2018, nine patients (including five males) with the middle and lower abdominal wall or groin region’s dermatofibrosarcoma protuberans received extended resection procedures in the Department of Plastic and Reconstructive Surgery of Sun Yat-sen Memorial Hospital, Sun Yat-sen University. The age distribution of patients was 27-60 years old with a mean age of 44.3 years old. The defect area following the extended resection of the tumor ranged from 9.5 cm × 10.5 cm to 15.0 cm × 18.0 cm, and the size of the ilioinguinal flap ranged from 6.0 cm × 11.0 cm to 8.0 cm × 15.0 cm. Either an axial flap or a V-Y advancement flap was used to repair the defect. Five patients were treated with the unilateral ilioinguinal flap, two with the bilateral ilioinguinal flap combination flap, one with the ilioinguinal flap combined with the tensor fascia lata myocutaneous flap, and one with the ilioinguinal flap combined with the anterolateral thigh flap.Results:All defects were covered by flaps completely, and all donor sites were closed directly. Eight flaps survived completely without necrosis, while one case of ilioinguinal flap faced the distal necrosis, which was debrided and repaired with full-thickness skin grafting in the second stage. The postoperative follow-up period ranged from 1.0 to 8.5 years. No local recurrence or other distant metastases occurred. All reconstructed regions had a good outline and ordinary sense function.Conclusions:Almost all defects after the extended resection of dermatofibrosarcoma protuberans in the middle and lower abdominal wall or the groin region could be repaired with a unilateral or bilateral ilioinguinal flap, whose blood supply is stable and reliable. Moreover, the operation presented is simple, less time-consuming, safe, reliable, and with a satisfactory postoperative appearance.

OBJECTIVETo analyze the correlation between mitral regurgitation grading and left ventricular ejection fraction in elderly patients (>60 years of age) in a 2-year follow-up.

METHODSA total of 455 patients with the diagnosis of at least mild mitral regurgitation by echocardiography were divided into ischemic mitral regurgitation (IMR) group and non-ischemic regurgitation (NIMR) group. The patients were followed up with echocardiography every 6 months and the data were analyzed at the end of 24 months.

RESULTSMitral regurgitation grade was inversely correlated with left ventricular ejection fraction (LVEF). Patients with moderate and severe IMR had a lower LVEF than those with NIMR (P<0.05). After adjustment for age, sex, body mass index, high blood pressure, diabetes, atrial fibrillation and cardiomyopathy, the mean LVEF at 2 years was lowered by 2.7% (1.4%-4.1%), 2.7% (1.3%-4.0%), and 5.2% (3.5%-6.9%) in mild, moderate and severe IMR patients, respectively (P<0.04), and by 3.2% (1.6%-4.8%), and 3.0% (1.4%-4.5%), and 1.7%(-0.5%-3.9%) in mild, moderate and severe NIMR patients (P=0.30).

CONCLUSIONThe mean LVEF in IMR patients is significantly lowered compared to that in NIMR patients. The grade of mitral regurgitation is inversely correlated with the regurgitation area in IMR patients. Stratified management might help improve LVEF in severe IMR patients.

Aged ; Aged, 80 and over ; Female ; Follow-Up Studies ; Humans ; Male ; Mitral Valve Insufficiency ; Stroke Volume ; Ventricular Dysfunction, Left ; physiopathology

Objective:To study the effect of lifestyle intervention and Irbesartan intervention on vascular endothelial function in elderly people with prehypertension.Methods:Seventy elderly people with prehypertension were randomly divided into the lifestyle intervention group and the Irbesartan intervention group.After one year of intervention, the level of cyclic endothelial microparticles(EMPs)and brachial-ankle pulse wave conduction velocity(baPWV)before and after the intervention were compared between the two groups.Results:There were significant differences in levels of circulating EMPs[(1 946±234)/μl vs.(1 745±416)/μl, t=2.530, P=0.011]and baPWV[(1 653±172)cm/s vs.(1 511±230)cm/s, t=2.448, P=0.017]before and after one year of lifestyle intervention.There were also significant differences in levels of circulating EMPs[(19 507±146)/μl vs.(1 446±286)/μl, t=3.340, P=0.002]and baPWV[(1 639±165)cm/s vs.(1 361±211)cm/s, t=3.156, P=0.005]before and after one year of Irbesartan intervention and between the lifestyle intervention group and the Irbesartan intervention group after one year( t=2.238 and 2.475, P<0.05). Conclusions:Lifestyle intervention and Irbesartan intervention have protective effects on vascular endothelial function and delay the initiation and progression of atherosclerosis in elderly people with prehypertension, and irbesartan intervention is more effective than lifestyle intervention.

Poststroke recrudescence (PSR) is a reappear phenomenon in the neurological symptoms of chronic stroke in the setting of toxic metabolic factors. Infection, hyponatremia, and use of benzodiazepine are important precipitants. In this paper, the risk factors, pathogenesis, clinical characteristics, diagnosis and treatment methods of PSR are reviewed to enhance the understanding of the disease among clinicians.