Objective:To explore the relationship among carotid intima‐media thickness (IMT) ,plaque score ,plasma level of C reactive protein (CRP) and coronary artery lesion in patients with coronary heart disease (CHD) .Meth‐ods:A total of 83 CHD patients were enrolled as CHD group and 37 patients without CHD were enrolled as non‐CHD group .CHD patients received coronary angiography .According to number of diseased coronary vessels ,CHD patients were divided into single vessel coronary disease group (n=28 ,single vessel group ) ,double‐vessel coronary disease group (n=31 ,double‐vessel group) and triple‐vessel coronary disease group (n= 24 ,triple‐vessel group) . Plasma CRP level and carotid IMT were measured ,and carotid atherosclerotic plaques were classified in all groups . Results :Compared with non‐CHD group ,there were significant rise in CRP level [ (0.89 ± 0.54) mg/L vs . (3.50 ± 3.42) mg/L] and IMT [ (0.65 ± 0.27) mm vs . (1.04 ± 0.31) mm] in CHD group (P<0.05 or <0.01);Com‐pared with single‐vessel group and double‐vessel group ,the CRP level of triple‐vessel group [ (1.44 ± 1.03) mg/L , (3.71 ± 3.52) mg/L vs .5.82 ± 3.98) mg/L] significantly rose ;the percentage of carotid plaque class 3 of triple‐ves‐sel group (0% 、0% vs .25% ) significantly rose (P<0.01 all) .Conclusion:C reactive protein level and carotid intima‐media thickness significantly rise in patients with coronary heart disease ,and there are significant rise in C reactive protein level and percentage of carotid atherosclerotic plaque class 3 in patients with multi‐vessel coronary disease .

Objective To observe the effect of ultra-low frequency transcranial magnetic stimulation on cerebral blood flow in children with spastic diplegia cerebral palsy. Methods 36 cases of spastic diplegia cerebral palsy were divided into control group (n=21) and obsevation group (n=15), 19 healthy children were as normal group. The control group received conventional rehabilitation training. The obsevation group received the ultra-low frequency transcranial magnetic stimulation in addition. All the children received the check of transcranial color Doppler (TCD). Results Several arterials flow velocity was lower, several arterials pulsatility index and resistance index were higher in the obsevation group than in the normal group before treatment (P<0.05). Compared with the control group, the arterial flow of obsevation group improved, pulsatility index and resistance index decreased in some degrees in the obsevation group after treatment (P<0.05). Conclusion The cerebral blood flow is of low velocity and high resistance in spastic diplegia children, while ultra-low frequency magnetic stimulation could improve the condition of cerebral flow.

Objective: To investigate the dynamic changes of antibodies induced by leptospiral vaccines. Methods: Antigens for antibody detection were screened out. ELISA was used to analyze antibody responses induced at different time points after immunizing guinea pigs with different batches of leptospiral vaccines from different manufacturers. To investigate the relationship between antibody responses induced by leptospiral vaccines and their protective effects in animal model, guinea pigs were challenged with Leptospira after immunization. Results: There was no significant antigen-antibody reaction between the LigA protein or Patoc Ⅰ antigen and the serum samples of guinea pigs immunized with leptospiral vaccines. Notable IgG and IgM antibody reactions were observed in all vaccination groups when using bacterial proteins from seven Leptospira reference strains which were used for the preparation of leptospiral vaccines as envelope antigens. Antigen-specific IgG antibodies peaked at 35 d after the last immunization, and the highest peak of antigen-specific IgM antibodies was reached 11 d after the last immunization. Results of the challenge test showed that non-diluted leptospiral vaccines induced significant IgG and IgM antibody reactions in guinea pigs as compared with those diluted three or nine times, showing good protective effects. Conclusions Analysis of the dynamic changes of antibodies induced by leptospiral vaccines revealed that there was correlation between the induced serum antibody responses and the protective effects. This study provided reference for further study on alternative methods for evaluating leptospiral vaccine potency.