DNAzymes generated by in vitro selection are single-stranded DNA molecules with catalytic capabilities. This article introduced the DNAzyme in such spects as structures, modifications, designing strategies, catalytic traits, advantages as well as its application as a new tool of gene therapy of tumor.

Objective:To investigate the infection status and influencing factors of hepatitis E virus infection among different occupational populations in Xinjiang region,and to provide a available evidence for control of hepatitis E.Methods:A randomly sampling survey was carried out in the population of public health practitioners, the serum IgG antibodies against hepatitis E virus in 1 720 samples were tested by indirect enzyme-linked immunosorbent assay,and the experimental data was analyzed with SPSS 19.0 software;the differences of infection rates between genders,ages and occupations were analyzed.Results:Among 1 720 subjects,440 persons were anti-HEV IgG positive,and the total positive rate was 25.58%;the positive rate of males (27.99%)was higher than that of females (23.35%),and the IgG positive rate was increased along with the age;there were significant differences of the anti-HEV-IgG antibody positive rates between the populations with different ages (χ2 =118.262, P = 0.000 ). There was a statistically significant difference of infection rate of hepatitis E between different occupational populations (χ2 = 98.262,P = 0.000),the slaughterman had the highest positive rate (81.53%), followed by the livestock and meat salesperson (79.37%),food service population (30%),supermarket population (11.21%)and others (9.03%).Conclusion:The hepatitis E infection rate is high among the public health practitioners in Xinjiang region,and occupation,age and gender are the major influencing factors of hepatitis E infection rate.

Objective To evaluate the infection rate and influencing factors of Hepatitis E Virus among different occupational population in nanjiang region of Xinjiang. Methods A cross-sectional survey was carried out in occupational population and non-occupational population having a close contact with livertock. Serum IgG antibodies against hepatitis E virus was tested by indirect enzyme-linked immunosorbent assay , and logistic regression model was used to analyze the risk factors of HEV infection. Results The result showed that the total positive rate was 51.88% and 317 out of 611 subjects were anti-HEV IgG positive. Non-occupational population had the lowest positive rate (35.90%), followed by the stock man of intensive nursery (24.14%), stock man of scattered nursery (76.00%), livestock and meat salesperson (79.37%) and slaughterman (81.53%). Multivariate logistic regression analysis showed that close contact with livestock , eating out and without frequent hand washing increased the risk of HEV infection . And HEV infection rate decreased in the population with professional training. Conclusion It is noticeable that promoting good health habits and cutting off animal-contact transmission are effective measures for the prevention of hepatitis E.

OBJECTIVE:To screen and analyze the ?-lactamases-resistant genes in imipenem-resistant pseudomonas aeruginosa (IMPRPa). METHODS: ?-lactamases-resistant genes including IMP,VIM and OprD2 in 60 clinically isolated IMPRPa strains were detected by polymerase chain reaction (PCR),and sequence analysis was performed on the PCR amplified products of some of IMP and VIM genes. RESULTS: Of the 60 IMPRPa strains,?-lactamases-resistant positive genes were as follows: IMP gene was positive in 7 strains,VIM gene was positive in 18 strains,and both IMP and VIM genes were positive in 3 strains. Sequencing of the PCR products of IMP and VIM genes were IMP-1 and VIM-2 types,and OprD2 gene deletion was noted in 29 strains. CONCLUSION: OprD2 protein channel deletion and metal-producing ?-lactamases are the chief mechanisms for the resistance of IMPRPa to imipenem.

OBJECTIVE:To explore the effects of Cangfu daotan decoction on expression of organic anion transporting poly-peptide(oatp4a1)and level of sex hormone in the endometrium and ovary tissues of rats with obesity-type polycystic ovarian syn-drome(PCOS). METHODS:Obesity-type PCOS rat models were established. 50 rats were randomized into a normal control(iso-metric normal saline)group,a model(isometric normal saline)group,a metformin(0.043 g/kg)group and Cangfu daotan decoc-tion high-dose and low-dose [5.68 and 1.42 g(medicinal materials)/kg] groups. The rats were given drugs ig once a day for 14 con-secutive days. The expression of oatp4a1 protein in the endometrium and ovaries of the rats was determined by ECL and Western blot. The levels of luteinizing hormone(LH),follicle stimulating hormone(FSH),testosterone(T)and estradiol(E2)in rat’s se-rum were detected by ELISA. RESULTS:Compared to the normal control group,those in the model group had weaker expression of oatp4a1 protein in the endometrium and ovaries,higher levels of serum LH,LH/FSH and T,lower levels of serum FSH and E2. There were statistical significances(P<0.01). Compared to the model group,those in metformin group,Cangfu daotan decoction high-dose and low-dose group had higher expression of oatp4a1 protein in the endometrium and ovaries,lower levels of LH/FSH and T,higher levels of serum E2;those in metformin group and Cangfu daotan decoction high-dose group had lower levels of serum LH,higher levels of serum FSH. There were statistical significances(P<0.01 or P<0.05). CONCLUSIONS:Cangfu daotan decoc-tion can increase the expression of oatp4a1 in the endometrium and ovaries of obesity-type PCOS model rat,alleviate phlegmy dampness and regulate the level of sex hormone.

Objective To evaluate the diagnostic efficiency of Q?SPECT, CTPA, Q?SPECT/CT, and Q?SPECT/CTPA for pulmonary embolism (PE) in rabbit models. Methods (1) The PE models were constructed by injecting Gelfoam into the femoral vein of New Zealand rabbits ( n=30) . Q?SPECT, CTPA, Q?SPECT/CT and Q?SPECT/CTPA fusion images were obtained by integrated SPECT/CT. (2) All images were interpreted by two experienced nuclear radiologists who were blind to pathologic findings. The locations and numbers of lung lobes with PE were recorded respectively. ( 3) Serial sectioning of the lungs was per?formed and pathologically determined. (4) Se, Sp and Ac of different methods were compared using McNemar test;PPV and NPV were compared usingχ2 test. Kappa test was used to analyze the consistency between two nuclear radiologists. Kappa values<0.40 were interpreted as poor consistency, 0.40 to 0.75 as moderate con?sistency, >0.75 as good consistency. Results (1) Histologically confirmed emboli were present in a total of 26 pulmonary lobes and absent in 79 lobes. (2)The Se, Sp, Ac, PPV, and NPV of 4 imaging methods were:53.8%(14/26), 93.7%(74/79), 83.8%(88/105), 14/19, 86.0%(74/86) for Q?SPECT;73.1%(19/26), 96.2%(76/79), 90.5%(95/105), 86.4%(19/22), 91.6%(76/83) for CTPA;76.9%(20/26), 93.7%(74/79), 89.5%(94/105), 80.0%(20/25), 92.5%(74/80)for Q?SPECT/CT;88.5%(23/26), 91.1%(72/79), 90.5%(95/105), 76.7%(23/30), 96.0%(72/75) for Q?SPECT/CTPA. (3) McNemar test showed Q?SPECT/CT and Q?SPECT/CTPA had higher diagnostic Se for the detection of PE than Q?SPECT (χ2=4.167, 7.111, both P<0.05) , but without any significant difference with CTPA in diagnostic efficiency (χ2=0-2.250, all P>0.05) . Q?SPECT/CT had higher diagnostic Ac than Q?SPECT (χ2=4.167, P<0.05) . There was no significant difference between Q?SPECT/CT and Q?SPECT/CTPA in diagnostic effi?ciency (χ2=0.001-1.333, all P>0.05). (4)Kappa values of 4 imaging methods for radiologist 1 and 2 were 0.902, 0.915, 0.973, and 0.884. Conclusions Q?SPECT/CT imaging provides good Se and Sp. The diag?nostic efficiency of Q?SPECT/CT is better than that of Q?SPECT and is corresponded roughly to the efficien?cy of CTPA, Q?SPECT/CTPA. The diagnosis of two radiologists on Q?SPECT/CT images has the best con?sistency.

Middle-aged women experienced foreign body sensation in both eyes without obvious causes for many days. The clinician tried to diagnose the patient according to her physical and laboratory examinations. After detecting worm bodies in the patient′s eye, and examining the worms with a microscope, the diagnosis of conjunctival sucking nematodes was confirmed. After targeted treatment, the patient′s symptoms improved significantly.