Objective To explore the necessity and training needs of nursing students before nurse qualification examination.MethodsTotally 200 undergraduates and college nursing students who graduated in 2016 were investigated during March to April 2016 by using stratified random sampling method. The questionnaire survey was applied to investigate the necessity,needs,and demand degree of training before examination.Results The scores of training necessity were (4.492±0.726) for undergraduates and (4.634±0.653) for college students (P>0.05). Both undergraduates and college nursing students had high training needs,which related to the circulatory system,respiratory system,digestive system,pregnancy, childbirth,and puerperal diseases (P>0.05). But college students were less desired to have nursing training of traditional Chinese medicine compared with undergraduates [(3.909±0.913) vs. (4.299±0.675);P<0.05]. Furthermore,142 (71%) participants preferred to have training on weekends;186 (93%) participants advised to combine teaching with exercise;92 (46%) participants recommended to invite both their own teachers and professors from other schools;88 (44%) participants thought that it was reasonable to remain two review weeks between the training and the exam.ConclusionsThe training before nurse qualification examination is necessary for nursing students. Students' knowledge level and course content should be paid attention to since only reasonable and effective training can help to improve their examination pass rate.

Objective To explore the plasma-activated medium(PAM)produced by low temperature plasma(LTP)on the proliferation and angiogenesis of human umbilical vein endothelial cells(HUVECs)so as to provide theoretical basis for the future use of PAM to promote wound healing and inhibit tumor angiogenesis.Methods HUVECs were selected as the in vitro research model.The PAM-containing medium after LTP treatment for different time points(0 s,15 s,30 s,45 s,60 s,and 75 s)was used for intervention.The influence of PAM on HUVECs viability was assessed using the MTT assay and cell cycle analysis.The effects of PAM on angiogenesis were examined through angiogenesis experiments.Intracellular levels of reactive oxygen species(ROS)were measured using fluorescence probes.A melanoma mouse model was established,and CD31 expression was detected by immunohistochemistry.Results As the treatment time increased,the intracellular levels of ROS also elevated.PAM derived from LTP exhibited a bidirectional effect on angiogenesis in HUVECs.Compared to the control group(0 s),low-dose treatments(15 s and 30 s)enhanced HUVECs viability,while high-dose treatments(45 s,60 s,and 75 s)significantly decreased cell viability(P＜0.05).The proportion of HUVECs in the S phase was significantly increased in the PAM-15 s and PAM-30 s groups,but markedly decreased in the PAM-45 s,PAM-60 s,and PAM-75 s groups,with statistically significant differences(P＜0.05).The HUVECs tube formation ability was enhanced in the 15 s and 30 s PAM groups,but diminished in the PAM-45 s,PAM-60 s,and PAM-75 s groups,characterized by the decreased numbers of vascular nodes,intersections,meshes,and branching points(P＜0.05).After PAM treatment in the melanoma mouse model,the control group exhibited widespread distribution of CD31 in tumor tissue,while the PAM-5 min and PAM-10 min groups displayed reduced distribution of CD31.Conclusion Short-term exposure to PAM enhances HUVECs proliferation and angiogenesis,whereas prolonged exposure suppresses cell viability and inhibits angiogenesis.

Objective To evaluate the clinical application effect of transparent film dressing in fistula nursing of patients with percutanueous nephrostomy (PCN).Methods By using before-after self control study method,40 patients with pelvic tumor that caused by postrenal renal failure,and needed long-term fistula remained after PCN surgery were selected as the research object.Conventional sterile gauze and tape were used to nurse the fistula before intervention and transparent film dressing was used after intervention.Results Patents always worried about trachea cannula exodus and infection before intervention.After intervention,the comfort degree of their daily life (like showering,sleeping and daily activities) was obviously higher than that before intervention (P＜0.01).The incidence of renal fistula local adverse effect after intervention is relatively low.And the difference was statistically significant (P＜ 0.01).Before intervention,the tape was loosing in (5.47 ± 1.48) days,there were three cases of accidental trachea cannula exodus;and after intervention,transparent film dressing hemmed in (14.22±1.99) days,and no accidental trachea cannula exodus occurred (P＜0.01);and no hyper-sensitive cases happened with the transparent film dressing.Conclusions The transparent film dressing has the advantages of stable viscosity,waterproofing,fungi-proofing,breathability and hypoallergenic,etc.It can well protect and fix the fistula,and it is suitable for nursing care of patients with long-term renal fistula.

Objective:To explore the application effect of micro-video combined with scenario simulation teaching in the training of orthopedic surgery posture placement for intern nursing students, so as to lay a foundation for improving the operation skills of nursing interns.Methods:A total of 86 nursing students in the operating room of Xijing Hospital of Air Force Military Medical University from June 2019 to June 2020 were selected as the research objects. They were divided into the control group ( n=42) and the intervention group ( n=44) according to the time of enrollment. The control group adopted the traditional clinical teaching and learning method, while the intervention group adopted the micro-video combined with scenario simulation teaching method. The effect of intervention was evaluated by theoretical assessment scores, skill operation assessment scores, medical education environmental scores and two-way teaching satisfaction. Results:The theoretical and technical performance assessment scores of the intervention group were higher than those of the control group ( P<0.05) . In addition, the correct rate of surgical positioning in the intervention group increased, while the time required to complete the positioning was reduced ( P<0.05) . In terms of medical education environment scores, students' perception of learning, students' perception of teachers, students' perception of the environment, and students' social self-perception scores and total scores of the intervention group were all higher than those of the control group ( P<0.05) . However, there was no statistically significant difference in academic self-perception of students between the two groups ( P>0.05) . At the same time, the two-way teaching satisfaction of nursing students and teachers in the intervention group was improved ( P<0.05) . Conclusions:The application of micro-video combined with scenario simulation teaching in the training of orthopedic surgical position placement of intern nursing students can effectively improve the teaching quality.

Objective:To analyze the application value of PAD (Presentation-Assimilation-Discussion) class and case teaching combined with brainstorming in the practice teaching of nursing students in the department of cardiovascular surgery.Methods:A total of 81 undergraduate nursing students who practiced in the Department of Cardiovascular Surgery, The First Affiliated Hospital of Air Force Medical University from June 2019 to April 2020 were selected as the research objects. They were randomly divided into the control group ( n=40, brainstorming teaching) and the observation group ( n=41, PAD class combined with case teaching). After one month of intervention, the teaching effect on the two groups of nursing students were evaluated through the nursing students' clinical communication ability scale, Chinese version of self-assessment of clinical reflection and reasoning (SACRR), and competency inventory for registered nurse (CIRN). And the teaching quality was assessed by the clinical nursing teacher teaching behavior assessment scale. SPSS 22.0 was used for t-test. Results:The scores of each dimension and total scores of clinical communication ability scale, Chinese version SACRR and CIRN of nursing students in the observation group were higher than those in the control group ( P<0.05). The scores of nursing teaching behavior evaluation of clinical teachers in the observation group were higher than those in the control group ( P<0.05). Conclusion:The combination of PAD class and case teaching with brainstorming can improve the clinical communication ability of nursing students in cardiovascular surgery practice, help to promote the transformation of their clinical thinking ability, improve their core competences, and play an important role in improving the teaching quality.

Objective To explore the effect of cognitive thinking rehabilitation nursing on the delayed encephalopathy after acute CO intoxication .Methods From October 2012 to May 2013 , totals of 37 cases with delayed encephalopathy after acute CO intoxication were selected as study group , and others 33 cases during November 2013 and May 2014 were recruited as control group .Study group received the rehabilitation nursing of cognitive thinking on the basis of routine nursing , while control group received the routine nursing only .Then, the clinical effect of two groups were observed .Results After the intervention , the scores of MMSE , HDS and ADL in study group were (16.87 ±3.95),(18.90 ±3.65) and (42.12 ±10.21),that respectively higher than before the intervention (25.04 ±2.00),(26.04 ±1.71) and (86.12 ±8.93),and the differences were statistically significant (P<0.05).In control group, scores of MMSE [(21.05 ±1.75) vs (16.86 ±3.92)], HDS[(20.97 ±1.65) vs (18.06 ±3.70)] and ADL[(65.52 ±10.38) vs (43.01 ±11.23)] after the intervention were higher than that before , and the differences were statistically significant (P<0.05).After the intervention , the scores of MMSE , HDS and ADL in study group were significantly higher than that in control group (t=3.449,6.747,4.758,respectively;P<0.05),and the nursing satisfaction of patients in study group was significantly higher than that in control group (97.30% vs 87.88%;χ2 =2.333, P <0.05). Conclusions Strengthen the rehabilitation nursing intervention of cognitive thinking can improve cognitive and self-care abilities of patients with delayed encephalopathy after acute CO intoxication , and improve patients ’ satisfaction,which is worthy of clinical popularization and application .

【Objective】 To observe the inactivation effect of a new low-temperature plasma air disinfector on microorganisms in the natural environment and artificial contaminated environment so as to further determine the minimum effective processing parameters. 【Methods】 First， in 0.5 m³ of super clean workbench and 1.05 m³ airtight organic glass cavity in the body， the new low-temperature plasma air disinfector developed was used to inactivate the natural bacteria in the air under different parameters. Then in 1.05 m³ airtight organic glass cavity in the body， the new low-temperature plasma air disinfector was used to treat the Staphylococcus aureus and Escherichia coli under the condition of artificial bacteria spraying. Last， the plate counting method was used to evaluate the inactivation effect of the disinfector on natural bacteria and artificial bacteria in the air. 【Results】 For the low-temperature plasma air disinfector in 0.5 m³ and 1.05 m³ confined space， the lowest effective treatment parameters for inactivated natural bacteria were 13 kV， 5 min or 12 kV， 10 min. For the low-temperature plasma air disinfection machine in 13 kV discharge voltage treatment of different time， compared with before treatment， the killing rate of Escherichia coli and Staphylococcus aureus increased significantly after treatment for 20 min （P<0.000 1）. Both could meet the requirements of disinfection specifications ［100%， （99.98±0.01）%≥99.90%］. 【Conclusion】 The inactivation of natural bacteria and artificial bacteria by the new low-temperature plasma air disinfector has reached the qualified standard of disinfection stipulated in Technical Specifications for Disinfection.

【Objective】 Escherichia coli phage (ECP) and Staphylococcus aureus phage (SAP) isolated from sewage were used as research objects, and their biological characteristics were analyzed to provide new experimental materials for the application of phages. 【Methods】 ECP and SAP were purified and cultured by double-layer agar method. Then a series of biological characteristics of these two phages were preliminarily analyzed by electron microscope observation, optimal multiplicity of infection (MOI) test, one-step growth curve test, temperature, pH, chloroform and ultraviolet sensitivity tests, respectively. 【Results】 The results of biological characteristics showed that ECP and SAP were both virulent phages, belonging to myoviridac family. Their optimal MOI was 10^-1, and they had strong resistance to ultraviolet light. The cleavage volume of ECP was 76.3 PFU/cell, while that of SAP was 8.3 PFU/cell. ECP had a wide range of temperature tolerance and could stably survive at 30-50 ℃, while SAP was more sensitive to temperature and could be completely inactivated at 50 ℃ for 1 h. ECP could maintain a good lysis activity in the range of pH 5-11, while SAP in the range of pH 6-9. ECP had strong resistance to chloroform and was non-membranous phage, while SAP was more sensitive to chloroform and was a membranous phage. 【Conclusion】 ECP and SAP are both virulent phages and have strong resistance to ultraviolet light. The lysability, temperature, pH, and chloroform tolerance of ECP are stronger than those of SAP.