Object To investigate the properties of layer crystal structure and its interlayer absorption and expansibility in montmorillonits clay lattice, which provide a basis for the montmorillonite as medicine adjuvant.Methods Montmorillonite sample was separated and purified from clay minerals, and the diameter of particle was less than 1 ?m in colloid, the setting film sample was ready-made, and the samples were analyzed with X-ray diffraction.Results The information of interlayer change pattern on montmori-llonite clay was obtained with different conditions.Conclusion Montmorillonite as swelling and adsorption clay minerals and form an interlayer absorption is possible and the X-ray diffraction can determine the amounts of layer distance.

Object The mineralogical and chemical compositions of the natural pharmaceutical montmorillonite clay were analysed with the aim of approaching the material base of its action Methods The means of X ray diffraction (SRD) and X ray fluorescence (SRF) were used for the quantitative analysis of the mineralogical and chemical compositions of the montmorillonite clay samples Results The essential mineralogical and chemical compositions of the six samples were obtained Conclusion Though there is some difference in mineralogical composition of the montmorillonite clay samples from different deposits, the average percentage of montmorillonite content in samples is 86%, and the chemical compositions of six samples are nearly similar

Objective: To understand secular trend of sedentary behaviors of school-aged children and adolescents in leisure time in China, and to provide theoretical basis for health behavior intervention. Methods: Data from China Health and Nutrition Survey (CHNS) during 2006 to 2015 were used to analyze leisure sedentary behaviors of children and adolescents aged 6-17 years. Means and medians were used for basic description and trend analysis, and nonparametric test was used for statistical comparison. Results: The total time of sedentary behavior during leisure time among children and adolescents aged 6-17 years showed a significant increasing trend, in which computer time contributed the most, especially among 6-11-year-old group with a five-fold increase. The time spent watching TV and doing homework slightly decreased. There was no sex difference in the total sedentary time during leisure time(Z=1.74，1.54，0.08，1.50，P>0.05), but there was sex difference in time spent in computer and homework(Z=2.00，2.01，2.84，2.92，P>0.05). Total sedentary time, as well as sedentary time spent in watching TV, computer, and homework differed by age and areas(Z=52.49-75.21，54.21-136.31，24.58-44.55; 6.25-8.61，6.42-13.34，3.89-6.42，P<0.01). Conclusion Over the last decade, sedentary behavior increased during leisure time among Chinese children and adolescents, and the usage pattern has also changed substantially. Results suggest that relevant departments should adopt more strategies to reduce sedentary behaviors and strengthen physical activities.

This research studies the status quo of credit system implementation in medical colleges and universities in Shandong Province, and finds that there are some problems, for examples, the number of courses can't meet the requirements of the credit system, teaching management is still difficult to adapt to the reform of the credit system, students lack guidance in choosing courses, and course resources are limited. Some suggestions are put forward, such as changing teachers' concept of teaching, intensifying teaching reform, increasing teaching resources input, promoting mutual recognition of credits, strengthening the construction of teaching staff, perfecting academic tutorial system, and constructing a credit system model that conforms to the laws of medical education.

Objective:To establish a colloidal gold technique assay for the rapid detection of immunoglobulin(Ig)M and IgG antibodies against 2019 novel coronavirus (2019-nCoV) and to evaluate its clinical performance.Methods:A total of 278 patients who were respectively treated at Wuhan Hankou Hospital and the People′s Hospital of Honghu from February 12, 2020 to February 20, 2020 were collected. According to the diagnostic criteria, 89 patients were confirmed with positive 2019-nCoV nucleic acid, and 189 were 2019-nCoV nucleic acid-negative suspected patients. A total of 273 medical examiners from Nanfang Hospital, Southern Medical University from 2015 to 2018 were selected as controls. The serum samples of patients were collected. 2019-nCoV nucleic proteins were obtained from prokaryotic expression vectors. Indirect IgM and IgG colloidal gold techniques were established by using recombinant nuclear protein. 2019-nCoV nucleic acid detection by reverse transcription-polymerase chain reaction (RT-PCR) was used as control. Serum specimens were tested for 2019-nCoV IgM and IgG. The specificity and sensitivity of colloidal gold assay were analyzed.Results:The positive rates of IgM and IgG with the colloidal gold detection in confirmed patients with positive 2019-nCoV nucleic acid were 78.7%(70/89) and 73.0%(65/89), respectively. The positive rates of IgM and IgG in medical examiners were 1.8%(5/273) and 0.7%(2/273), respectively. The sensitivity and specificity of IgM detection reagents were 78.7% and 98.2%, respectively, those of IgG detection reagents were 73.0% and 99.3%, respectively, and those of IgM combined with IgG detection were 87.6% and 98.2%, respectively. For suspected patients with negative 2019-nCoV nucleic acid, the positive rates of IgM and IgG were 59.8%(113/189) and 52.9%(100/189), respectively, and the positive rate of IgM combined with IgG detection was 66.1%(125/189).Conclusion:This reagent of 2019-nCoV antibodies detection (colloidal gold technique) fulfills the requirement for clinical application with high specificity and sensitivity, which can be served as a supplementary detection method for 2019-nCoV nucleic acid detection by RT-PCR.

Objective: To establish a colloidal gold technique assay for the rapid detection of immunoglobulin(Ig) M and IgG antibodies against 2019 novel coronavirus (2019-nCoV) and to evaluate its clinical performance. Methods: A total of 278 patients who were treated at Wuhan Hankou Hospital and the People's Hospital of Honghu from February 12, 2020 to February 20, 2020 were collected. According to the diagnostic criteria, 89 patients were confirmed with 2019-nCoV nucleic acid positive diagnosis, and 189 were 2019-nCoV nucleic acid-negative suspected patients. A total of 273 medical examiners from Nanfang Hospital, Southern Medical University from 2015 to 2018 were selected as controls. The serum samples of patients were collected. 2019-nCoV nucleic proteins were obtained from prokaryotic expression vectors. Indirect IgM and IgG colloidal gold techniques were established by using recombinant N protein. 2019-nCoV nucleic acid detection by reverse transcription-polymerase chain reaction (RT-PCR) was used as control. Serum specimens were tested for 2019-nCoV IgM and IgG. The specificity and sensitivity of colloidal gold assay were analyzed. Results: The sensitivity and specificity of IgM detection reagents were 78.7% and 98.2%, respectively, those of IgG detection reagents were 73.0% and 99.3%, respectively, and those of IgM combined with IgG detection were 87.6% and 98.2%, respectively. For suspected patients with negative 2019-nCoV nucleic acid, the positive rates of IgM and IgG were 59.8% (113/189) and 52.9% (100/189), respectively, and the positive rate of IgM combined with IgG detection was 66.1% (125/189). Conclusion This reagent of 2019-nCoV antibodies detection (colloidal gold technique) fulfills the requirement for clinical application with high specificity and sensitivity, which can be served as a supplementary detection method for 2019-nCoV nucleic acid detection by RT-PCR.