Objective: To investigate the status and relationship between anxiety and family need among parents of infants admitted to NICU, and examine the moderating effect of perceived nurse support on its relationships. Methods: A convenience sample of 207 parents were selected from the Second Hospital of Harbin Medical University during December 2017 to October 2018. To adopt the measurement of socio-demographic, Self-rating Anxiety scale (SAS), NICU-Family Need Iventory, (NICU-FNI) and Nurse Parent Support Tool (NPST) assessed the general information, anxiety, family need and perceived nurse support respectively. Results: The score of SAS were (57.81±3.83). SAS was positively correlated with family need (r= 0.327, P< 0.01) and negatively correlated with perceived nurse support (r=-0.294, P<0.01). The multiple linear regression analysis showed that perceived nurse support could moderate the relationships between family need and anxiety (β=-0.154, △R²= 0.018, P=0.012). Conclusions Severity anxiety were occured in parents of infants admitted to NICU. The perceived nurse support could moderate the relationships between family need and anxiety. To reinforce perceived nurse support and enhance satisfaction of family need by intervention strategies could be an effective approach to improve psychological adjustment among parents of infants admitted to NICU.