BACKGROUND:Umbilical cord stem cel s mainly derive from ful-term infants, and common culture methods include tissue-attached method and trypsin-digestion mehod. However, effects of different culture methods on the separation of umbilical cord mesenchymal stem cel s remain many disputes. OBJECTIVE:To observe the effects of different culture methods on umbilical cord mesenchymal stem cel s. METHODS:Umbilical cords of 30 healthy ful-term and caesarean delivery infants were selected, and cultured using tissue-attached method or trypsin-digestion method to isloate and culture human umbilical cord mesenchymal stem cel s. Meanwhile, cel growth was measured by flow cytometry. RESULTS AND CONCLUSION:The fusiform-shaped cel s began to separate from the umbilical cord tissue that was primary cultured using tissue-attached method, and 10 days later, the cel fusion reached 80%;after the umbilical cord was cultured using col agenase-trypsin digestion for 5 days, a smal amount of adherent cel s with different shapes appeared, and the fiber-like cel s reached 80%of confluence until 2-week culture. There was no significant difference in the growth of umbilical cord mesenchymal stem cel s cultured by different culture methods (P>0.05). Moreover, cel s cultured by two methods were al positive for CD13, CD29, CD44 and CD105. These results demonstrate that human umbilical cord mesenchymal stem cel s exhibit a high success rate in primary culture using tissue-attached method, which is superior to the trypsin-digestion method.

In bacterial cells, selection of the proper division site at midcell requires the specific inhibitions of septation at two other potential sites, locate at each of the cell pole. This site specific inhibition of septation is mediated by the gene products of the min locus including three genes: minC, minD, and minE. Genetic and expression studies have revealed that MinC encode an inhibitor of division that is activated by MinD and toplogically regulated by MinE. Recent localization studies of functional Min proteins tagged with green fluorescent proteins have provide some insight into this topological regulation and revealed a fascinating oscillation of MinC and MinD between the cell halves. In this paper, it is reviewed that the progress on the regulation mechanism of the division site in bacterial cells by introducing the structure and their interaction of the study on Min proteins.

Objective To explore the pathological mechanism of brain function and structure in young patients with major depressive disorder by diffusion tensor imaging(DTI)and resting state functional magnetic resonance imaging(fMRI).Methods Sixteen participants diagnosed with major depression (MD) and 16 healthy age and gender-matched controls(HC) were recruited. Resting state fMRI and DTI brain scans were performed on all participants. A voxel-based method (VBM) was used to analyze the DTI datasets, and regional homogeneity (ReHo) approach was applied to preprocess the fMRI datasets. The value of fractional anisotropy (FA) and ReHo maps were obtained in the whole brain.Results FA values in the MD group were significantly lower than those of the healthy controls in the white matter of the left middle temporal gyrus, right middle frontal gyrus, left medial frontal gyrus, right precentral gyrus, left angular gyrus, left fusiform gyrus, left superior temporal gyrus, right middle temporal gyrus, right sub-gyral, left insula, and left pyramis (P＜0.01). ReHo in the MD group decreased in the left superior frontal gyrus, right superior frontal gyrus, left middle frontal gyrus, right middle frontal gyrus, left medial frontal gyrus, right medial frontal gyrus, left paracentral lobule, right paracentral lobule, right inferior parietal lobule, left postcentral gyrus, right postcentral gyrus, left middle occipital gyrus, left lingual gyrus, right putamen, right cingulate gyrus, right cuneus, left superior temporal gyrus, and right middle temporal gyrus (P＜0.01). Conclusion Abnormality of brain white microstructure and function exist widely in young patients with major depressive disorder. Abnormal connection of structures and function between the brain areas may be the key reason for the depression.

0.05).Conclusion Donepezil hydrochloride do not improve the learning and memory function of normal under age rats.

Objective To explore the effect of Acanthopanax senticosus injection (ASI) on brain multi-infract dementia rats. Methods Thirty-six male Wistar rats were randomly divided into 3 groups:control group, model group and ASI group. The multi-infract dementia rat models were set up by injecting mini-sludged blood in carotis internal arteries, learning and memory function of rats were determined by Morris water maze and Step-down test, the section of rat cerebral cortex and hippocampus were stained with haematoxylin eosin (HE). Results Compared with model group, latency in ASI group shortened significantly at 2nd,5th and 6th day, the distance shortened at 1st,2nd,5th and 6th day. The seeking tactics of ASI trgated rats improved in the Morris water maze test. The error times of ASI treated rats decreasd at 1st and 2nd day in Step-down test; ASI did not reduce significantly pathological changes of vascular dementia rats. Conclusion ASI has effect of treatment on multi-infract dementia rats.

Objective To explore the mechanism of therapeutic effect of Donepezil hydrochloride on Alzheimer's disease(AD) rats.Methods According to weight,36 rats were divided into normal group,model group and Donepezil hydrochloride group.AD rat model was set up by injecting D-galactose into abdominal cavity for seven weeks,learning and memory function of rats was determined by using Morris water maze and Step-down test.The section of rat cerebral cortex and hippocampus were stained with haematoxylin eosin(HE),and the effect of Donepezil hydrochloride was observed by detecting the MDA content and SOD activity in cerebral tissue.Results Compared with model group,latency and distance of Donepezil hydrochloride rats shortened on the fourth day and the fifth day,starting angle of Donepezil hydrochloride rats shortened on the fourth day and the fifth day in the Morris water maze test,error times of Donepezil hydrochloride rats decreased on the first day and the second day in Step-down test;MDA content in cerebral tissue of Donepezil hydrochloride rat was deceased(P

Objective To observe the influence of XueShuanXinMaiNing(XSXMN)in the behavior and structures of cerebral cortex and hippocampus of the rats withβamyloid protein(Aβ)-induced Alzheimer’s disease(AD),and to explore its therapeutic effects on the rat AD.Methods 100 male Wistar rats were selected.According to weight, the rats were randomly divided into sham operation group, model group, positive drug group (donepezil hydrochloride,1.75 mg· kg-1 ),XSXMN 1.1 g· kg-1 group and XSXMN 2.2 g· kg-1 group. The rat AD models were made by injecting Aβinto hippocampus.After oral administration for 15 d,Morris water maze test, dark avoidance task and pathology test were performed.Results In Morris water maze test,compared with model group,the latency and swimming distance to platform of the rats in XSXMN 1.1 g·kg-1 group were decreased on the 2nd,4th and 5th day(P<0.05 or P<0.01);in XSXMN 2.2 g·kg-1 group,the latency to platform of the rats were decreased from the 3nd to 6th day(P<0.05 or P<0.01),the swimming distances to platform of the rats were decreased from the 3rd to 5th day(P<0.05 or P<0.01).On the 7th day,in XSXMN groups,the times of passing platform,time of staying on platform,distance of staying on platform,time of staying in effective area, distance of staying in effective area, time of staying on platform/total time, distance of staying on platform/total distance,time of staying on platform/total time were all increased significantly(P<0.05 or P<0.01)within 90 s. In dark avoidance task,compared with model group,the error latency and the error times of the rats in XSXMN groups had no obvious change on the 2nd day.The pathological results showed that there were degeneration nerve cells and necrosis nerve cells in the rat cerebral cortex in XSXMN groups,while in the rat hippocampus there were less number of nerve cells with obscure cell layer and many degeneration and necrosis cells were found;compared with model group,there was no obvious improvement.Conclusion XSXMN can improve the learning and memory function of the AD rats.

OBJECTIVE： To study the pharmacokinetics of domestic carvedilol and relative bioavailability of carvedilol capsule in Chinese volunteer.METHODS： Eight volunteers orally took a single dose of 30mg test preparation and 25mg control preparation in a random crossover and self-control method.Samples were determined by RP-HPLC fluorescent method.RESULTS： Profiles of carvedilol in vivo could be described as open two-compartment model.The main pharmacokinetics parameters of test and control preparations were as follows： Cmax（98.89± 27.60） ng/ml、 （70.06± 27.29） ng/ml， Tmax（0.4 849± 0.2 635） h、 （0.6 037± 0.1 707） h， CL（0.1 621± 0.08 057） （mg· h） /（ng· ml） 、 （0.1 796± 0.09 198） （mg· h） /（ng· ml） ， V/F(c)（0.2 127± 0.1 260） mg/(ng· ml)、 （0.2 777± 0.1 860） mg/（ng· ml） ， T1/2β （2.011± 1.709） h、 （1.959± 1.156） h， AUC（233.1± 97.12） ng/（ml· h） 、 （168.0± 70.61） ng/（ml· h） ； Mean relative bioavailability in man was (111.3± 15.18)％ .CONCLUSION： The results can be used for design of therapeutic scheme.

Objective To compare the effect of active compression-decompression cardiopulmonary resuscitation(ACD-CPR) with standard- cardiopulmonary resuscitation(S-CPR) on ventricular function in a canine ventricular fibrillation model. Methods Ventricular fibrillation was induced in anesthetized and instrumented canine. Twenty-four dogs were randomly assigned to either ACD-CPR group or S-CPR group.After 4 minutes of untreated VF,two-dimension echocardiography was used to evaluate the left ventricular end-diastolic volume(LVEDV),left ventricular end-systolic volume(LVESV) and left ventricular ejection fraction (LVEF) of every canine of the two groups when they were undergoing cardiopulmonary resuscitation. Results During ventricular fibrillation, both ACD-CPR group and S-CPR group showed decreased LVEDV compared with pre-ventricular fibrillation, but not statistically significant( P ＞0.05).LVEDV was increased in ACD-CPR group compared with S-CPR group, but not statistically significant (P＞ 0. 05). Both ACD-CPR group and S-CPR group showed significantly increased LVESV compared with pre-ventricular fibrillation,of which the difference was statistically significant ( P ＜0. 001). Both ACD-CPRgroup and S-CPR group showed significantly decreased LVEF compared with pre-ventricular fibrillation,of which the difference was statistically significant (P ＜0. 001). LVEF was increased in ACD-CPR group compared with S-CPR group,of which the difference was statistically significant ( P = 0.019). Conclusions Compared with S-CPR,ACD-CPR resulted in higher LVEF.

The linear analysis for heart rate variability (HRV), including time domain method, frequency domain method and time-frequency analysis, has reached a lot of consensus. The non-linear analysis has also been widely applied in biomedical and clinical researches. However, for non-linear HRV analysis, especially for short-term non-linear HRV analysis, controversy still exists, and a unified standard and conclusion has not been formed. This paper reviews and discusses three short-term non-linear HRV analysis methods (fractal dimension, entropy and complexity) and their principles, progresses and problems in clinical application in detail, in order to provide a reference for accurate application in clinical medicine.
Electrocardiography ; Entropy ; Fractals ; Heart Rate ; Humans