Objective:To understand the status of overweight and obesity in healthy people in Shenyang area, and to explore the related factors of overweight and obesity and the influence of overweight and obesity on biochemical indicators such as blood lipid and blood glucose level.Methods:A total of 6 065 healthy subjects in our physical examination center of Shengjing Hospital Affiliated to China Medical University from January 2019 to October 2019 were collected and divided into normal group, overweight group and obesity group according to different BMI.The distribution characteristics of overweight and obesity in different gender, age were analyzed, as well as the influence of overweight and obesity on blood glucose, blood lipid and other indicators.Logistic regression was served to analyze the influence factors of overweight and obesity.Results:Among the 6 065 subjects, the prevalence of normal, overweight and obesity was 39.50%, 41.85% and 18.65%, respectively.The prevalence of overweight and obesity tended to rise with the increase of age(χ 2=118.958, P<0.001). The overweight and obesity rates of men(47.45%, 24.64%) were higher than those of women(33.08%, 9.26%), and the difference was statistically significant (χ 2=577.176, P=0.000). The levels of blood glucose, total cholesterol, triglyceride, low-density lipoprotein cholesterol, apolipoprotein B, creatinine, uricacid, cystatin C, glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase, gamma-glutamyltransferase, alkaline phosphatase, prealbumin, systolic blood pressure and diastolic blood pressure in the overweight/obesity group were higher than those in the normal group, and the levels of high-density lipoprotein cholesterol and apolipoprotein A1 were lower than those in the normal group, the differences were statistically significant( t=13.904, 7.668, 0.057, 9.710, 19.723, 12.840, 27.840, 15.880, 8.368, 22.986, 18.382, 10.924, 17.283, 23.378, 8.033, 29.782 and 18.937, all P<0.001). Logistic regression analysis showed that the risk factors of overweight/obesity were age, gender, systolic blood pressure, triglyceride, blood glucose, apolipoprotein B, uricacid, cystatin C, glutamic-pyruvic transaminase, gamma-glutamyltransferase.High-density lipoprotein cholesterol, apolipoprotein A1, creatinine, glutamic-oxalacetic transaminase were the protective factors of overweight/obesity(Wald=37.863, 6.871, 40.632, 13.472, 16.800, 28.165, 94.152, 9.533, 126.332, 8.017, 16.427, 34.338, 13.948, 49.814, all P<0.01). Conclusion:The prevalence of overweight/obesity of the healthy people in Shenyang is high.We should take intervention measures as early as possible to improve the quality of life.

Klebsiella pneumoniae(K.pneumoniae)is an important pathogen that can cause severe hospital-and community-acquired infections.To systematically investigate its methylation features,we determined the whole-genome sequences of 14 K.pneumoniae strains covering varying serotypes,multilocus sequence types,clonal groups,viscosity/virulence,and drug resistance.Their methy-lomes were further characterized using Pacific Biosciences single-molecule real-time and bisulfite technologies.We identified 15 methylation motifs[13 N6-methyladenine(6mA)and two 5-methylcytosine(5mC)motifs],among which eight were novel.Their corresponding DNA methyl-transferases were also validated.Additionally,we analyzed the genomic distribution of GATC and CCWGG methylation motifs shared by all strains,and identified differential distribution pat-terns of some hemi-/un-methylated GATC motifs,which tend to be located within intergenic regions(IGRs).Specifically,we characterized the in vivo methylation kinetics at single-base resolu-tion on a genome-wide scale by simulating the dynamic processes of replication-mediated passive demethylation and MTase-catalyzed re-methylation.The slow methylation of the GATC motifs in the replication origin(oriC)regions and IGRs implicates the epigenetic regulation of replication initiation and transcription.Our findings illustrate the first comprehensive dynamic methylome map of K.pneumoniae at single-base resolution,and provide a useful reference to better understand epigenetic regulation in this and other bacterial species.