Objective To investigate the protection of Rhodioloside on brain tissue in diabetic rats. Methods The rats were randomly divided into six groups: a normal control group, a model control group, a Rhodioloside high, medium and low dose group, and a positive control group(n=16). Except normal control group, the diabetic models were set up by intraperitoneal injecting STZ(60 mg/kg). And then, the Rhodioloside high, medium, low dose group were respectively given Rhodioloside solution(100、50、25 mg/kg) by intragastrical administration(ig), the positive control group was given metformin hydrochloride(25 mg/kg) by ig, the normal control group and positive control group were given normal saline(NS). Before the drug was given and 4, 8, 12 weeks after, the level of blood sugar was determined. And 12 weeks later, the body weight was detected; the activity of CPK, LDH and the level of T-AOC in serum were determined; the activity of SOD, GSH-Px, CAT and the content of MDA in brain tissue were determined; and the histopathological changes of the brain tissue was observed by HE staining.Results Compared with the model control group, the level of blood sugar (16.0 ± 0.9 mmol/Lvs. 18.0 ± 1.2 mmol/L) in Rhodioloside high dose group was significantly decreased (P<0.01), the activity of CPK(240.7 ± 62.3 U/Lvs. 312.5 ± 78.2 U/L) and LDH (265.4 ± 54.6 U/Lvs. 346.3 ± 73.8 U/L) in serum were significantly decreased (P<0.01), the level of T-AOC (10.8 ± 2.8 U/Lvs. 8.7 ± 2.4U/L) was significantly increased (P<0.01); the activity of SOD(11.5 ± 3.2 U/mgvs. 8.1 ± 2.9 U/mg), GSH-Px(13.1 ± 2.8 U/mgvs. 11.4 ± 2.5 U/mg), CAT(3.8 ± 1.1 U/mgvs. 2.6 ± 0.8 U/mg) in brain tissue were significantly increased (P<0.05,P<0.01), and the content of MDA (5.3 ± 1.4 U/mgvs. 6.3 ± 1.8 U/mg) in brain tissue were significantly decreased (P<0.01), which were better than Rhodioloside low dose group; but compared with positive control group, the difference was not significant (P>0.05). The brain histopathological changes of Sal treated groups were significantly improved, while the treatment effect of Rhodioloside high dose group was the most significant.Conclusions Rhodioloside had dose-dependent protective effect on the brain tissue in diabetic rats, whose mechanism perhaps related to its effects on enhancing the activity of antioxidant enzymes, and reducing the damage of oxidative stress.

Objective To study relationship between blood-glucose change and prognosis of the patients after severe traumatism.Methods 70 cases severe traumatic emergency patients selected tested the level of blood-glucose instantly when they presented themselves to the clinic after injury,used saccharimether and blood-glucose test paper provided by the Johnson Limited Corporation,and 30 slight traumatic emergency patients at the same time were selected as contrel.Result The blood-glucose after severe traumatic were markedly higher than that of slight traumatic group,which have significance(P

Objective To explore the relationship between the family conditions and internet addiction of medical college students with limb disabled. Methods 400 medical college students were investigated by the University Student Going-online Investigation table, EMBU and Chinese Internet Addiction Scale. Results There was significant correlation between parental occupations and internet addiction total score of medical college students with limb disabled( r= 0.195, P < 0.01 ; r = 0.163, P < 0.05 ). There was significant correlation and prediction between negative parental rearing patterns such as the parents' punishment-severity and rejection-denying and dimensions of interpersonal relations, health, forced going-online behaviors, time management( P < 0.05, P< 0.01 ), but there was no significant correlation and prediction between parents' warmth-comprehension and all dimensions. Conclusion Family conditions play a important role in influencing internet addiction of medical college students with limb disabled.

AIM: To investigate the effects of losartan on myocardial fibrosis and left ventricular hypertrophy in hypertensive patients. METHODS: 35 essential hypertensive patients with left ventricular hypertrophy were treated with losartan 50-100 mg for 6 mon. Before and after the treatment, serum procollagen typeⅢ (PCⅢ), laminin (LN), and hyaluronic acid (HA) were measured by radioimmunoassay; and left ventricular mass index (LVMI) was determined with ultrasonic cardiogram. RESULTS: After treatment with losartan: the serum levels of PCⅢ, LN, HA, and LVMI were all decreased (P

In this paper,we present analysis,servi-ce and study for zero grating in H-300.Transmission electron microscope made in Japan.It is well known that this kind of electron microscope have been used in most university and institute over twenty years, so all most of the electron microscope have problem in its dependability.In order to analyze and service the electron microscope,we make much efforts in the material chosen and the service technics,the experimental results show that our methods are available for servicing those electron microscope. Moreover,we discuss the failure of the electron microscope and the jointing crafts,these results are useful for those who works in this field.

BACKGROUND: Caspase family is viewed as the executive factor of cell apoptosis. Neuronal apoptosis happens probably after spinal cord injury.OBJECTIVE: To observe the changes in caspase-3 expression after spinal cord injury in rats so as to probe into the relationship between it and neuronal apoptosis and provide the evidence on the prop e r time window of intervention on alleviating secondary spinal cord injury.DESIGN: Self-control and mutual-control were designed in animal experiment.SETTING: Department of Traumatic Surgery and Department of Orthopedics of Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology.MATERIALS: The experiment was performed in Experiment Room of Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology from January to December 2001, in which, 54SD rats were employed, of either sex, mass weighted varied from 220 to 250 g and provided from Animal Experimental Center of Tongji Medical College of Huazhong University of Science and Technology.rats were divided into the control and injury group. Laminectomy was only done on Ts and T9 in the control and the injury group was subdivided into 9 subgroups, in which, the materials were collected on the 4th and 8th hours and on the 1st, 2rd, 3rd, 7th 14th and 21st days successively, 6 rats in each one. After abdominal anesthesia with 30 g/L pentobarbitol sodium,sternal cord on T8 andT9 segments were exposed with Nystrom method and 50 g weight compressed the front middle region of the spinal cord of such segments with arch smooth metal pad 2.2 mn×5.0 mm for 5 minutes. After injury, artificial bladder urination was done 3 times at 10:00, 16:00and 22:00 successively everyday till the bladder reflex was established.cord was collected at various time spots after spinal cord injury. 4 pieces of spinal cord tissue masses from each group, about 8mm in length, were embedded with paraffin and sectioned continuously. Afterwards, HE staining, immunohistocheistry and TUNEL (TdT-mediated dUTP-biotin nick end labeling) were performed successively. Two rats were sacrificed on ice in each group and central tissue of injured spinal cord was placed in expression was assayed with immunohistochemistry method, neuronal apoptosis was assayed with TUNEL method and linear correlation was used to analyze the correlativity between caspase-3 expression and neuronal apoptosis.pase-3 expression after spinal cord injury in rats of each group.RESULTS: Six rats were maintained in each group and included in result optic microscope: Extensive hemorrhage appeared in 1 hour in injured segment. In 4 to 8 hours, spinal structure began destructive and a large amount of neuronal death appeared. In 24 hours, the destruction of spinal cord became severe and in 7 to 21days, the range of injury was defined with immunohistochemistry in rats of each group: Very few caspase-3 expressions (2.1±0.5) presented in neurons of spinal cord in normal rat. In 8hoursafter spinal cord injury, caspase-3 expression of positive neurons was increased remarkably (89.2±10.5) and up to the peak (189.6±12.7) in 3 days. Caspase-3 expression of positive cell and apoptotic cell appeared alexpression assayed with transcription-polymerase chain reaction (RT-PCR)in rats of each group: Caspese-3 mRNA (0.442±0.024) began increased in 4h, was up to the peak (0.634±0.028) in 48 hours and was restored to be normal (0.351±0.013) in 7 days, which appeared early than apoptosis, indicating positive correlation with the level of neuronal apoptosis (r=0.622).In the control and 4 hours group, stained cell was seen occasionally and positive cell appeared 8 hours later, mainly localized in gray matter. Afterwards, positive cell was increased and up to the peak in 3 days. In 7 days,positive cell of apoptosis and staining was decreased gradually in gray matter, mainly around the white matter. Little amount positive cells appeared on the 14th day and 21st day.CONCLUSION: In normal spinal cord tissue, caspase-3 existed in form of zymogen with very low activity. Caspase-3 is enhanced in expression after spinal cord injury in rats, expresses in large amount in 8 hours and is up to the peak in 24 to 48 hours, which is overlapped in time with positive apoptotic cell assayed with TUNEL and concerning to the localization, it is in conformity with positive apoptotic cell of spinal cord injury compared with positive cell of caspase-3. It is indicated that caspase-3 is involved in regulation of cell apoptosis after spinal cord injury. It is seen in this experiment that the time from spinal cord injury to the activation of caspase-3 is the time window of treatment for cell apoptosis intervened by spinal cord and alleviating secondary spinal cord injury. It is suggested that genetic intervention or specific caspase-3 inhibitor should be applied in 48 hours.

Background and purpose:Runt-related transcription factor 3 (RUNX3) has a signiifcant relation with gastric cancer. Many researches have confirmed that rs760805 AA can increase the risk of gastric cancer. The purpose of the study was to investigate the relationship between the rs760805 polymorphism of the RUNX3 gene and gastric cancer. Methods: The rs760805 genotypes were determined by PCR-based DNA sequence measuring analysis and direct DNA sequencing in 310 incident cases with gastric cancer and 327 controls recruited in Shandong. Results:The frequency of TT genotype was 15.16%in gastric cancer patients and 20.49%in normal controls, and the corresponding percentages for AT and AA genotypes were 48.39%and 36.45%, and 52.60%and 26.91%, respectively. Compared to TT genotype, AT genotypes were associated with an increased risk of gastric cancer (OR=1.24, 95%CI`:0.81-1.92;OR=1.83, 95%CI:1.15-2.92). Conclusion:The rs760805 polymorphism of the RUNX3 gene is associated with increased susceptibility to gastric cancer.

Objective To investigate the relationship between infarct location and prognosis after emergency percutaneous coronary intervention (PCI) treatment in patients with acute myocardial infarction.Methods Consecutive 121 patients with ST elevation myocardial infarction (STEMI) who underwent primary PCI were divided into 3 groups according to the infarct location.Their clinical,ECG,echocardiography and coronary angiography features were analyzed,and the follow-up was made. Results Compared with inferior wall infarction group and inferior wall infarction combined with right ventricular infarction group,anterior wall myocardial infarction group had higher CPK-MB and cTnT,less frequent collateral,reduced ST-segment resolution,higher mortality rates in hospitalization and one year after discharge.On the other hand,inferior infarction combined with right ventricular infarction group had more occurrences of shock and atrial vetricular block or ventricular arrhythmia,more incidence of multivessel disease and thrombus,and higher readmission rate.Conclusion Patients with anterior wall myocardial infarction and inferior wall in

Objective To study the diagnostic value of porphobilin staining of gastric mucus for primary pathologic duodenogastric reflux (DGR). Methods A total of 58 DGR patients diagnosed from January, 2007 to April, 2008 were recruited to the study as DGR group, and 21 healthy volunteers as control.All subjects underwent 24-hour intragastric bilirubin monitor and gastroscopy. Bilirubin absorption value of 0. 25 and median reflux time of 23.60% were taken as thresholds to differentiate low reflux group ( reflux time ＜ 23.60% ) and high reflux group (reflux time ≥23.60% ). Porphobilin staining of gastric mucosa was quantitatively analyzed. Results Deposition of porphobilin in mucosa of gastric antrum, gastric angle and gastric body in primary pathologi DGR group was significantly higher than those in healthy group (P ＜0. 05 ). The occurrence of atrophic and intestinal metaplasia of gastric antrum in high reflux group was significantly higher than that of low reflux group (P ＜ 0. 05). Deposition of porphobilin in mucosa of gastric antrum, gastric angle and gastric body in high reflux group was significantly higher than that of low reflux group (P ＜ 0. 05 ). The New Sydney system pathological scores of gastric antrum and angle of high reflux group was higher than that of low reflux group ( P ＜ 0. 05 ). The deposition of porphobilin in mucosa of gastric antrum and gastric angle was positively correlated with New Sydney system pathological scores in primary pathological DGR group (r=0.59, P=0.041 andr=0.73, P=0.038). Conclusion Porphobilin staining of mucosa in gastric antrum can reflect the severity of bile reflux, and is positively correlated with the extent of gastric mucosal lesion, which may be helpful in diagnosis of primary pathological DGR.

Inner ear is one of the most sensitive parts to microwave radiation.The effect of microwave radiation on the inner ear system is its damage to the vestibule and cochlea.The vestibular and cochlear injury is closely related to the balance of the human body and hearing loss.This article reviews the mechanism of microwave and effects of microwave radiation on the structure and function of the inner ear in order to provide data for future related research.