Objective To observe the reductov of the 3-d Elastic Exteral Fixation of Calcaneal.Methods based on the bearing capacity of foot structure,the anatomic form of Calcaneal was restored in short time by the combination of acting and reacting forces formed by the pressor effect of pressure stalk.Results The excellent and good rates of treatment result were above 97.8%.Condusion It does not shield the X-ray aswell as safe and reliable which makes it possible to investigate the positions of the fractures.

AIM:To investigate the regulatory role of hypoxia mimic reagent cobalt chloride ( CoCl2 ) on cave-olin-1 (Cav-1) generation and the influence of Cav-1 on the abilities of migration and invasion of human lung adenocarcino-ma A549 cells.METHODS:The concentrations of Cav-1 and hypoxia-inducible factor ( HIF)-1αin pleural effusion of the patients with lung cancer ( MPE) or tuberculous pleurisy ( TBPE) were detected, and the correlation was also compared. A549 cells were treated with CoCl2 at different concentrations and time in the presence or absence of HIF-1αinhibitor YC-1.The concentrations of Cav-1 and HIF-1αin the cell supernatants were measured by ELISA.The effects of Cav-1 induced by CoCl2 on the migration and invasion of A549 cells were determined by scratch test and Transwell invasion trial, respec-tively.RESULTS:The levels of Cav-1 and HIF-1αin MPE were significantly higher than those in TBPE.There was a highly positive correlation between Cav-1 and HIF-1αlevels in the pleural effusion.CoCl2 induced the generation of Cav-1 and HIF-1αin A549 cells in a concentration-and time-dependent manner, the peak occurred at 200 μmol/L or 24 h, while the concentration over 200 μmol/L or after treated over 24 h, a concentration-or time-dependent inhibition was ob-served.HIF-1αinhibitor YC-1 concentration-dependently inhibited the generation of HIF-1αand Cav-1 induced by CoCl2 in A549 cells.CoCl2 enhanced A549 cells migration and invasion, with 200 μmol/L played the strongest role, which were down-regulated significantly in the presence of YC-1.CONCLUSION:The alteration of hypoxia-induced Cav-1 generation might be involved in the migration and invasion of A549 cells.A possible role for HIF-1αis indicated in Cav-1 generation.

AIM: To explore the effect of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism. METHODS: Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression level of linc00467 in the plasma of LAD patients and healthy volunteers. The overall survival (OS) was analyzed by Kaplan-Meier survival analysis and log-rank tests. Cell proliferation assays and Xenograft mouse model were used to confirm the effect of linc00467 expression on tumorigenesis in vitro and in vivo.RESULTS: linc00467 expression was up-regulated in the plasma of LAD patients compared with healthy volunteers. In addition, high levels of linc00467 expression were correlated with larger tumour sizes, lymph node metastasis and advanced TNM stages. High levels of linc00467 indicated a poor prognosis in LAD patients, multivariate analyses indicated that linc00467 expression could serve as an independent prognostic factor for overall survival of LAD. Functional experiments showed that knockdown of linc00467 could inhibit LAD cell proliferation in vitro and in vivo. CONCLUSION: linc00467 is involved in the progression of LAD and that linc00467 may be a novel diagnosis biomarker and a potential therapeutic target for LAD.

Objective To study the differential diagnostic value of interferon-γ inducible protein 10 (IP-10),macrophage inflammatory protein-1 α (MIP-1 α) and monocyte chemoattractant-1 (MCP-1) level in the tuberculous,malignant pleural effusion.Methods Enzyme-linked immunosorbent assay was used to detect the level of IP-10,MIP-1 α and MCP-1 in tuberculous pleural fluid (tuberculous pleural fluid group,43 cases) and malignant pleural fluid (malignant pleural fluid group,45 cases).The level of IP-10,MIP-1 α and MCP-1 and the significance were analyzed by ROC curve.Results The level of IP-10,MIP-1 α and MCP-1 were significantly higher in tuberculous pleural fluid group than those in malignant pleural fluid group,and there were significant differences(t =4.931,3.106,2.385 ; P =0.000,0.004,0.041).ROC curve analysis showed that the critical value of IP-10,MIP-1 α and MCP-1 in diagnosis of pleural effusion was respectively 1 589.73,213.50,1 452.63 ng/L.The sensitivity and specificity of IP-10,MIP-1 α and MCP-1 in pleural fluid were 68.8％,81.3％,87.5％ and 87.5％,68.8％,56.3％,respectively.Conclusion The level of IP-10,MIP-1 α and MCP-1 in tuberculous and malignant pleural fluid are significant for the early diagnosis and differential diagnosis.

Objective To investigate clinical effects of g rafting of vascular sural nerve.Methods From1998to 2003,97cases of the defects o f the distal ulnar artery and nerve we re repaired by grafting of sural nerv e with anastomosis of short saphenous vein.Results All the cases were followed up for 6to24months.They were e-valuated according to the modified S eddon rating system.The total excellent and good rate was 80.41%.The ex-cellent and good rate of the emergency cases was 89.74%,while the excelle nt and good rate of second operation was74.14%.Conclusions Grafting of sural nerve with anastom osis of short saphenous vein is an effective method to repair the defects of distal ulnar artery and nerve of the forearm,because the procedure is easy,the nerve and vein are widely available,and the nerve is th ick enough to be foldable.The artery can be repaired after reverse vascul ar anastomosis so as to improve the bloo d circulation of limbs and prevent nerve necrosis.[

ObjectiveTo discuss the epidemiological characteristic s of road traffic injuries in the Qinghai-Tibet Plateau and explore effective pre ventive measures against traffic accidents in the plateau as well as correspondi ng emergency treatment methods.MethodsA retrospective statis tical analysis was carried out in 1 894 cases of traffic injuries with detailed data admitted in our hospital from January 1980 to December 2000. Resu ltsMost of the traffic accidents occurred on the Qinghai-Tibet road, accounting for 68.0% (1 288/1 894). Of the patients with serious traffic inju ries, 54.0% (1 022/1 894) were in need of hospitalization. The patients who first entered into the plateau accounted for 61.1% (1 158/ 1 894). Death occurre d in 108 cases accounting for 5.7%. The duration from injury to treatment was ve ry long, average over six hours. Furthermore, the patients received no any manag ement before treatment in the hospital. ConclusionsHypoxia i n the plateau, unclear road sign and fatigue driving are the main factors leadin g to traffic accidents. Lack of health care units, serious hypoxia, long deliver y time and carriers' deficiency in medical knowledge are the main causes for gr eat amount of casualties.

Objective To study the diagnosis and treatment of blunt pancreatic injury (BPI). Methods A retrospective analysis was made on the clinical data of 58 cases of BPI admitted and treated in our hospital during 23 years. Results The positive diagnosis rate was 51.1%(23/45) for single BUS examination ,but 70.0%(21/30) for two and more BUS examimations. The positive diagnosis rate was 65.0%(26/40) for single CT scanning, but 91.3%(21/23) for two and more CT scannings. Twenty-five cases(25/37,67.6%) with BPI were determined preoperatively, and 12 cases(12/37,32.4%)during (operation).Thirty-seven cases underwent operatiom, included grade I in 10cases、grade II 13cases、grade III 9cases、 grade IV 4cases and gradeV 1case. Twenty-one cases received nonoperative treatment, included 11 grade in I,7 grade II and 3 grade III. In the entire group, 6 cases died(10.4%).The mortality in the operation group was 16.2%(6/37).There were no deaths in the nonoperative group. The main cause of death was multiorgan failure(5/6,83.3%). There were 11(19.0%)cases of pancreatic pseudocyst after treatment in the entire group, included 3(3/37,8.1%) in operation group and 8(8/21,38.1%) in nonoperation group. Conclusions The diagnosis of BPI should combine the clinical findings with repeated BUS and CT scanning. Nonoperative treatment is a good choice for BPI without main pancreatic duct injury. Operative treatment is suitable for serious BPI and those with other intra-abdominal organ jnjury.

The etiology of deep vein thrombosis (DVT) is still not elucidated nowadays. Based on the accordance between DVT incidence and the anemophilous pollen concentration in the air, we proposed the hypothesis that allergic reaction induced by anemophilous pollen may cause "idiopathic" DVT, and proinflammatory factors may play an important role in the thrombosis process.

AIM: To investigate the role of Rho-kinase signal pathway in rat cardiac fibroblasts (CFBs) proliferation and collagen synthesis induced by angiotensinⅡ (AngⅡ). METHODS: CFBs of neonatal Sprague-Dawley (SD) rats were isolated with the method of trypsin digestion and differential anchoring velocity. The CFBs were stimulated with AngⅡto induce fibrosis. Proliferation of CFBs was observed by MTT coloricmetric assay. Synthesis of collagen was detected by the hydroxyproline. The expression of Rho-kinase mRNA was examined using RT-PCR analysis. The extent of phosphorylation of myosin-binding subunit (MBS-P) of myosin phosphatase was quantified by Western blotting analysis, which was used to evaluate the activity of Rho-kinase.RESULTS: (1) Stimulation of neonatal SD rat CFBs with AngⅡ (10-7 mol/L) significantly increased CFBs proliferation and collagen synthesis (P

Drug and radiofrequency ablation were used to treat atrial fibrillation ( AF) . Because the drug therapy is often empiri?cal, and the side effects of drug can leads to many adverse reactions of heart and heart outside. Patient?s compliance is poor after long term medication, so the therapeutics effect is limited. At present, catheter ablation is the main therapy for the treatment of AF, and 2014 AHA/ACC/HRS guidelines has been recommended for the treatment of AF in the first line. But there is a certain recurrence af?ter ablation. There are many factors that cause the recurrence of AF, such as left atrial size, heart rate, PR interval, inflammatory factors. Accurate prediction of recurrence of AF after catheter ablation is helpful for early identification of high?risk groups, so as to take relevant preventive measures.