Carcinoma, Hepatocellular ; enzymology ; pathology ; Glutathione Peroxidase ; Humans ; Liver Neoplasms ; enzymology ; pathology ; Thioredoxin-Disulfide Reductase

Objective To investigate the role of Trx-1 and Trx-2 in the progression of hepatitis B associated acute-on-chronic liver failure (ACHBLF).Methods We determined the mRNA levels of Trx1,Trx-2 in peripheral blood mononuclear cells (PBMC) from a cohort of 60 patients with ACHBLF,38 patients with chronic hepatitis B (CHB) and 26 healthy controls by fluorescence real-time quantiative PCR.Results We demonstrated that there were significantly higher expressions of Trx-1 and Trx-2 mRNA in patients with ACHBLF than those patients with CHB and healthy controls (P ＜ 0.05,respectively).Furthermore,the levels of Trx-1 and Trx-2 mRNA significantly correlated with total bilirubin (TBIL) levels in patients with ACHBLF (P ＜ 0.05,respectively).The relative expression of Trx-1 mRNA significantly correlated with model for end-stage liver disease (MELD) scores in patients with ACHBLF (P ＜ 0.05).Besides,Trx-1 and Trx-2 levels were higher in non-survivors with ACHBLF than survivors with ACHBLF (P ＜0.05,respectively).The relative expression of Trx-1 mRNA was predictive of 90-day mortality using Kaplan-Meier analysis (P ＜ 0.05),with a sensitivity of 75％ and a specificity of 64.29％.Conclusion Trx and associated oxidative stress may play an important role in the pathogenesis and progression of ACHBLF,the mRNA level of Trx-1 and Trx-2 may be associated with disease severity and poor prognosis of ACHBLF.