Objective To compare RNA interference (RNAi) with imatinib in killing K562 cells. Methods Design effective shRNA sequences special for bcr-abl silencing and insert them into the eukaryotic expression vector for RNAi by gene engineering. The recombinant plasmi(ts were then transfected into K562 cells. 48 hours later, the efficiency of transfection was identified by fluorescent microscope, bcr-abl mRNA level was detected by RT-PCR. Another group of K562 cells were treated respectively by imatinib with different concentration. All groups of K562 cells were finally analyzed in apoptosis, cell proliferation and phosphotyrosine-containing proteins. Results Both RNAi and imatinib induced apoptosis, decreased proliferation and reduced phosphotyrosine-containing proteins. Conclusion BNAi can kill K562 cells successfully as imatinib, and it may be a promising way to treat CML patients in clinic, especially for those who fail in imatinib or other chemotherapy.

Objective To investigate the impact on the mental state and quality of life of positive cognitive behavioral therapy in breast cancer patients undergoing chemotherapy. Methods A total of 81 patients with breast cancer after chemotherapy were selected from May 2013 to November 2014 in our hospital, all of them were divided into two groups,the control group had 41 cases,they used chemotherapy treatment, the observation group had 40 cases, they were given positive cognitive behavioral therapy on the basis of the chemotherapy drugs,the anxiety was assessed with SAS, depression was assessed with SDS, quality of life of breast cancer patients were assessed with the quality of life scale (FACT-B),the intervention effect of two groups was compared. Results The degree of anxiety and depression of the observation group after the intervention were significantly lighter than the control group,the difference was signifi-cant(P<0.05). The physiological conditions, social/family status, emotional status, functional status, additional atten-tion scores and QOL score of the observation group were higher than the control group, the difference was significant (P<0.05). Conclusion Chemotherapy in breast cancer patients with positive cognitive behavioral therapy after treatment can effectively relieve patient anxiety,depression and other negative emotions,improve the quality of life,can clinical application.