Objective To investigate clinical characteristics of paediatric allergic rhinitis .Methods 133 paediatric patients with allergic rhinitis were divided into preschool group and school-age group ,and analyzed the clinical characteristics .Results The pro-portion between preschool group and school-age group with nasal discharge ,nasal blockage ,associated with coughing ,preschool group has significantly difference with school-age group(P<0 .05);the proportion between preschool group and school-age group with mild intermittent ,moderate to severe persistent ,preschool group has significantly difference with school-age group(P<0 .05);The proportion of dietary factors between two group is significant (P<0 .05) .The proportion of relation between intermittent ,mild persistent ,environmental change ,other ,affect morpheus quality ,difficult to concentrate ,and cognitive decline between the two had no statistical significance (P> 0 .05) .Conclusion Compared the clinical manifestation between preschoolers and school-age chil-dren ,we got some clinical data of paediatric allergic rhinitis patients ,those were beneficial to the diagnose and therapy of paediatric allergic rhinitis .

Objective To statistically summarize the adverse reactions of anti-TB drugs treatment,to analyze the influencing factors of leucopenia and to compare the effect of Qijiao Shengbai Capsule and Leucogen Tablet in the treatment of leucopenia.Methods The adverse reactions in 840 TB patients diagnosed from September 2014 to November 2015 were statistically analyzed.The influencing factors of leucopenia were analyzed.Finally,the effect of Qijiao Shengbai Capsule(n=480) and Leucogen Tablets (n=360) for treating leucopenia.Results Clinicians paid less attention to routine blood monitoring (39.4 ％).Among adverse reactions,the damage of liver function had the highest incidence rate(abnormal liver enzymes 27.0％,increased bile acid 8.2％),followed by leukopenia [25.4％,mainly degree Ⅰ (16.9％)];female patients(P=0.000) and complicating liver damage(P=0.010) might be the risk factors of leukopenia.After treatment by two kinds of drug,white blood cells count was increased,the difference was statistically significant(P=0.000).Conclusion The occurrence rate of leucopenia is higher,females and liver function damage are easier to appear,so more attention should be paid to monitoring the blood routine in return visit.The two kinds of drug for treating leucopenia induced by anti-TB drugs have definite effect and could be used in clinic.

Objective To investigate the hemostatic effects and safety of the QuGouCao chloroform-water extract.Methods The hemostatic property of the extract was studied by applying the extract soaked gauze to the local wound surface of skin,liver and femoral artery in rabbits.LD50 test in Kuming mice was performed and the tissues of liver and kidney were collected for routine histochemical examination.The skin stimulus in rabbits and the sensitivity test in guinea pigs were conducted.Results The hemostatic time of wounds was obviously shorter(P

Objective To screen and analyze genes up regulated in human umbilical vein endothelial cells (HUVEC) stimulated by lipopolysaccharide (LPS). Methods Suppression subtractive hybridization (SSH) was performed between the unstimulated HUVEC(driver) and HUVEC stimulated with LPS(tester) to generate subtractive cDNA library. The library was screened with colony dot hybridization to further verify the differentially expressed cDNA clones. Positive clones were sequenced and BLAST analyzed. The 3 novel cDNA sequences were verified by RT PCR. Results Twenty five up regulated genes related to inflammation, cellular cytoskeletal rearrangement, cellular proliferation and apoptosis, intercellular message transduction, and 3 new expression sequence tags (EST) were acquired. RT PCR indicated the expression of the new ESTs only in HUVEC stimulated by LPS. Conclusion SSH is a powerful technique of high sensitivity for the detection and clone of up regulated gene expressed in HUVEC stimulated by LPS, which may be helpful to clarify the mechanism of endothelial cells activation stimulated by LPS.

Objective The recombinant fluorescent eukaryotic expressing vector containing hTERT cDNA was transfected into human embryonic fibroblasts (hEFs) to explore the effects of exogenous hTERT on the type I and III collagens expression in hEFs. Methods p IRES2-EGFP-hTERT plasmid and pIRES2-EGFP plasmids were transfected into primary hEFs respectively by Lipofectin reagent. Expression of type I and III collagen was determined by Western blotting and the content of type I and III collagens in the cellular medium at 3 days after transfection were examined by radio-immunoassay. Results The expression levels of type I and III collagens in hTERT gene transfected hEFs(hEF-hTERT) were obviously higher than those in untransfected hEFs and vacant vector transfected hEFs(hEF-EGFP). The content of type I and III collagens in the cellular medium in hEF-hTERT cells at 3 days after transfection was also higher than that in untransfected hEFs and hEF-EGFP cells. Conclusions The synthesis ability of type I and III collagens in hEFs could be promoted by exogenous hTERT gene transfection.

To screen genes in endothelial cells resulted from responding to lipopolysaccharide (LPS), mRNA was extracted from both untreated human umbilical endothelial cells (HUVEC) following and HUVEC which were treated with LPS for 6 hours. cDNAs of both populations were synthesized to generate cDNA libraries by suppression subtractive hybridization (SSH). The libraries were then screened with colony dot blots. Positive clones were sequenced and BLAST analysed. The results showed differential genes included 3 novel genes and 22 known genes. The 3 novel genes were confirmed by Northern blotting analysis. These 22 known genes were involved in the regulation of proinflammatory response, cell apoptosis, cytoskeleton, signal transduction and energy metabolism. These results suggest that SSH is an effective technique to detect differential gene expression in HUVEC, which may be helpful to evaluate molecular mechanisms of endothelial injury induced by LPS and provide potential therapeutic targets for LPS related disturbances.

In order to explore the effects of exogenous human telomerase reverse transcriptase (hTERT/hTRT/hEST2) on telomeric restriction fragment (TRF), telomerase activity and its subunits expression in human embryonic fibroblasts (hEFs), hTERT sense eukaryotic expression vector pIRES2 EGFP hTERT was constructed with DNA recombinant technique and then transfected into primary hEFs by Lipofectin method. TRF length, telomerase activity and changes in telomerase subunits expression were examined and evaluated in transfected and untransfected cells. The results showed that telomerase activity in pIRES2 EGFP hTERT transfected cells (hEF EGFP) was significantly higher than that in untransfected hEFs and vacant vector transfected cells (hEF EGFP) ( P

Objective To study the subcellular localization and the tissue expression of EOLA1(endothelial-overexpressed lipopolysaccharide-associated factor 1). Methods The fusion protein EOLA1-EGFP expressed vector was constructed and transfected into endothelial cells. After 24 hours posttransfection, the subcellular localization of EOLA1 was detected by laser-scanning microscopy. The tissue-specific distribution of EOLA1 was assessed with Multiple Tissue Northern Blots. Results The expression of EOLA1 was tissue-specific in various human tissues. With human multiple tissue Northern blot analysis, it was shown that EOLA1 could express in the heart, skeletal muscle, kidney, liver, placenta, colon, spleen, small intestine, but did not in the brain, lung, thymus, and peripheral blood leukocyte. EOLA1 was mainly localized in cytoplasm and could move into the nucleus. Conclusion EOLA1 is one of intercellular proteins and may play a role in intercellular signal transduction.

Objective To study the synthesis of methyl bryoanthrathiophene-1-carboxylate with high efficiency. Methods Target compounds were synthesized from 1,8-dihydroxy-9,10-anthraquinone via 5 steps including protecting, nitration, methyl mercaptoacetate substitution, intramolecular Aldol reaction and deprotecting. Results Target molecule was synthesized in the total yield of 45.8%. Conclusion Our synthesis of methyl bryoanthrathiophene-1-carboxylate is characterized by short synthetic route, high selectivity and high efficiency.

Objective To design and synthesize a new potential angiogenesis inhibitor, demethyl bryoanthrathiophene. Methods Target molecule-demethyl bryoanthrathiophene was synthesized in 5 steps using 1,8-dihydroxy-9,10-anthraquinone as the starting material, 5,7-dihydroxy-1-carboxyl-6-oxo-6H-anthra thiophene as the key intermediate and decarboxylization as the key step. Results The target molecule was synthesized with a total yield of 10.1% and identified by MS, IR, UV, 1H-NMR, 13C-NMR and 2D-NMR. Conclusion Our synthesis of demethyl bryoanthrathiophene is based on an easily-acquired starting material and accomplished with a simple synthetic route.