Objective To investigate the effect of extraction of radix isatidis on the expression of transforming growth factor(TGF-β)in lung tissues of rats with radioactive lung injury.Methods 72 female Wistar rats were randomly divided into 3 groups,blank control group(groupA,n=24),simple irradiation group(group B,n=24),irradiation with Chinese medicine group(group C,n=24).Rats in group A and group B were given distilled water 2 mL/200g by gavage for one week,the rats in group C were given extraction of radix isatidis 2 mL/200g(1.6 g/200 g)per day for one week,then the rats received irradiation,during irradiation,the rats' weight was measured once a week,to illuminate the end.After 10-time irradiation(the 30th day), and at 60 days and at 120 days after irradiation,the expression levels of TGF-βin lung tissue were detected;In addition each rat lung tissue morphology was observed.Results The expression levels of TGF-βprotein in group B reached peak at the 30 days after irradiation,which at different time points in group A and group C was significantly lower than that in group B(P<0.01).Rats of group A had no significant change in lung tissue.Rats of group B were observed inflammatory cells in interstitial pulmonary alveolar,alveolar structural damaging,interstitial pulmonary edema,interstitial hemorrhage and inflammatory pulmonary with alveolar cavity;Rats of group C pulmonary interstitial fewer inflammatory cells in alveolar damage were not obvious, with no-pulmonary interstitial edema, hemorrhage and no inflammatory symptoms, comparison between group B and C was not obvious. Conclusion Extraction of radix isatidis could reduce the expression levels of transforming growth factor(TGF-β)in lung tissues of rats with radioactive lung injury ,has better preventive effect on radioactive lung injury,could protect lungtissue from damaging,inhibite alveolar permeability,and present the effect of pulmonary edema.

Objective To make molecular diagnosis for puerile spinal muscular atrophy(SMA).Methods Genomic DNA was extracted directly from the blood of both the case group(10 children with SMA) and the control group(including 19 parents of SMA patients and 20 healthy individuals).Two methods,polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and allele-specific PCR,were used to analyze exon 7 of SMN gene from genomic DNA,and consequent electrophoresis of PCR products on agarose gel was performed.Results Genotyping results obtained by both methods were in complete agreement for all of the samples analyzed.In conventional PCR-RFLP,part of the PCR products(189bp) from genomic DNA of all 39 members in the control group remained intact after digestion with Dra I,while the PCR products from genomic DNA of all 10 SMA children in the case group was completely digested by Dra I.In allele-specific PCR,exon 7 of both SMN1 and SMN2 could be seen when genomic DNA of all 39 members in the control group was used,while only SMN2's exon 7 could be seen when genomic DNA of all 10 SMA children in the case group was used.Conclusion Homozygous deletion of SMN1 was present in all 10 SMA children in the case group,while homozygous deletion of SMN1 was not detected in all 39 members in the control group.The combination of PCR-RFLP and allele-specific PCR,both their results can be references for each other,offers efficient and accurate methodology for molecular diagnosis of SMA.

Child ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy

Humans ; Hydrocarbons, Chlorinated ; poisoning ; Propane ; poisoning

Objective To investigate the clinical manifestations and diagnosis and treatment of aortic dissection(AD).Methods The clinical data of 20 patients with aortic dissection were analyzed retrospectively.Results 20 patients were diagnosed by CT, MRI and colour ultrasonography (5,5,10 cases, respectively). According to DeBakey typing, this group of patients was composed of type I(11 cases, 55%), type II(2 cases, 10%)and type III (7 cases, 35%). All patients were treated with internal medicine ,average time in hospital stay was(29 8?25 5)days. The patients' conditions were improved in 19 person-times (76%). 4 patients died (20%) and 2 patients abandoned treatment (8%).Conclusions The accurate diagnosis as early as possible and active therapy was a key of improvment of prognosis in patients with aortic dissection.

Objective To investigate the properties of the serum free binding of vascular endothelial cells(ECs) to lipopolysaccharide (LPS). Methods The binding of ECs to fluorescein isothiocyanate labelled LPS(FITC LPS) was observed with laser confocal microscope(LCM). The extract of ECs membrane after binding with FITC LPS was recorded with fluorescence microscope. Results The binding of ECs to FITC LPS was in a dose dependent manner. The fluorescence was mainly distributed in cytoplasm, especially near the nucleus which could also be stained. The bound FITC LPS had a very close relationship with the membranous system of ECs. Conclusion ECs could bind to LPS directly and could function intracellularly.

Acute Disease ; Esophageal Stenosis ; chemically induced ; Herbicides ; toxicity ; Humans ; Paraquat ; toxicity ; Poisoning

Humans ; Neurotoxicity Syndromes ; Paraquat ; toxicity

Acute Disease ; Humans ; Oxalates ; poisoning

Mother-to-child transmission (MTCT) is a major route of transmission of syphilis,and may occur at any time during pregnancy.MTCT of syphilis can lead to many adverse pregnancy outcomes,seriously affects maternal and infant health,and has been a severe public health and social problem.The risk of MTCT of syphilis is associated with stage of syphilis in pregnancy,stage of pregnancy,receiving or not receiving treatment,and is especially high in patients with early syphilis.With the growth of incidence of syphilis,the prevention for MTCT of syphilis has been becoming more and more important.Screening for and early treatment of syphilis in pregnancy can effectively block MTCT of syphilis.To learn the epidemiology,route,risk,and associated factors of MTCT of syphilis will undoubtedly facilitate the development of strategies for syphilis prevention and control.