Objective To evaluate the clinical anatomy for repair the deformed of later facial paralysis in power by grouped human specimen of the blood vessel and nerve bunch of temporal muscle Methods Materials consisted of 20 fresh human temporalis muscle specimens Divided to four groups in random Vascular infusion was by either solution of lead oxide,methylmethacrylate or Zhonghua ink,and stained the microdissection of nerve All the images were made photograph,and overlaped by computer To form the bunches of vascular and nerve Results Temporal muscle have three bunches to utilize independently,and the blood vessel and nerve formed a dense interlacing network Conclusion To power correct deformed in eye,nose and mouth of later facial paralysis,we can accomplish once a time with the corresponding temporal muscle bunches of vascular and nerve

Objective To summarizes the clinical experience of the arterial switch operation (ASO) for the complex congenital heart defects with ventriculoarterial connection anomaly. Methods Between January 2000 and August 2004, 60 consecutive neonatal and infants, aged 2 days to 11 years with mean body weight (6.5?4.3)kg (2.6～22 kg), underwent the arterial switch operation at Fu Wai hospital, including 42 patients in early years (2000.1～2003.5), and eighteen patients in recent years (2003.6～2004.8). Primary cardiac diagnoses included transposition of the great arteries (TGA, n=49) and Taussing-Bing anomaly (TBA, n=7), and the congenitally corrected transposition of the great arteries (ccTGA, n=4). The operation was performed under general anesthesia and extracorporeal circulation with the low temperature and low volume blood flow. The great arteries were transected above the valvular commissures; the coronary ostia with all the adjacent sinus of valsalva were excised and reimplanted to the proximal neo-aorta. The proximal neo-pulmonary trunk was reconstructed with a large autologous native pericardium as a pantatoon patch. The pulmonary anastomosis was completed after the aortic cross-clamp was released. The VSDs were repaired through the atrium or proximal aorta with dacron patches. For the patients with ccTGA, double switch operation included Senning procedure and ASO were performed. Results The total operative mortality rate was 16.7% (10 cases), 9 cases in early years (21.4%, 9/42) and 1 case in recent year (5.6%, 1/18), two groups had significant difference (P

Diabetes and lung cancer are common diseases worldwide , which have a serious impact on human health .Recently epidemiologic research has found that diabetes , which may enhance the incidence of lung cancer , is one of the risk factors of lung canc-er.The metformin, a first-line drug for treatment of type 2 diabetes, can decrease the incidence of lung cancer and may have an anti-lung-cancer bioactivity ,but its mechanisms are unclear .To make a further study of the mechanisms is expected to reduce the morbidity and is possible to help us find a new therapeutic target .The aim of this article is to review the potential mechanism of the metformin′s anti-lung-cancer activity .

Objective To evaluate the efficacy and feasibility of ultrasound-guided percutaneous microwave ablation for peripheral lung cancer. Methods Seventeen patients with peripheral lung cancer (23 nodules) were treated with ultrasound-guided microwave ablation. The microwave energy was initially set at 60 W for 100s, and then at 30~40 W for 300~600 s. The treatment was conducted by using multiple simultaneously-powered antennas in lesions ≥3 cm in diameter. Results After the treatment, all the nodules were decreased in size and the blood flow signals inside the nodule disappeared (14 nodules) or weakened (9 nodules). Enhanced CT scanning revealed no enhancement in 16 nodules and partial enhancement in 7. Repeated biopsy in 4 patients showed a complete tumor necrosis. Clinical symptoms disappeared in 8 patients and subsided in 9 patients 1 month after treatment. Follow-up observations in 15 patients for 6~47 months (mean, 23 months) showed 9 survivors, without serious complications. Conclusions Ultrasound-guided percutaneous microwave ablation is an effective, safe, and feasible method for treating peripheral lung cancer.

Objective To statistically summarize the adverse reactions of anti-TB drugs treatment,to analyze the influencing factors of leucopenia and to compare the effect of Qijiao Shengbai Capsule and Leucogen Tablet in the treatment of leucopenia.Methods The adverse reactions in 840 TB patients diagnosed from September 2014 to November 2015 were statistically analyzed.The influencing factors of leucopenia were analyzed.Finally,the effect of Qijiao Shengbai Capsule(n=480) and Leucogen Tablets (n=360) for treating leucopenia.Results Clinicians paid less attention to routine blood monitoring (39.4 ％).Among adverse reactions,the damage of liver function had the highest incidence rate(abnormal liver enzymes 27.0％,increased bile acid 8.2％),followed by leukopenia [25.4％,mainly degree Ⅰ (16.9％)];female patients(P=0.000) and complicating liver damage(P=0.010) might be the risk factors of leukopenia.After treatment by two kinds of drug,white blood cells count was increased,the difference was statistically significant(P=0.000).Conclusion The occurrence rate of leucopenia is higher,females and liver function damage are easier to appear,so more attention should be paid to monitoring the blood routine in return visit.The two kinds of drug for treating leucopenia induced by anti-TB drugs have definite effect and could be used in clinic.

Objective To investigate the effect of high-flux hemodialysis on mineral and bone metabo-lism( MBD) in diabetic kidney disease patients with maintenance hemodialysis .Methods 60 cases with diabetic kidney disease undergoing maintenance hemodialysis were studied and they were randomly divided into two groups according to the number table method .Patients in each group were treated using either high-flux dialyzer or low-flux dialyzer for 6 months.Blood calcium(Ca), blood phosphorus(P), calcium*phosphorus(Ca*P), alkaline phosphatase(AKP), parathyroid hormone(PTH), and 25 hydroxy vitamin D3(25-OH-D3),etc were deter-mined at the start and end of the study .Results In high flux dialysis group , serum P, serum Ca*P, and PTH levels was(1.52 ±0.50)mmol/L,(3.05 ±1.19)pg/L and(368.61 ±235.32)pg/L respectively, which was sig-nificantly decreased than before treatment ((1.78 ±0.55)mmol/L,(3.94 ±1.31),(427.45 ±288.93)pg/L) (P<0.05), while serum Ca, and 25-OH-D3 levels were(2.3 ±0.16)mmol/L and(21.64 ±8.51)nmol/L respectively, which were increased significantly than before treatment ((2.12 ±0.18)mmol/L,(16.77 ±7.69) nmol/L)(P<0.05), yet in low flux dialysis group serum Ca, P, AKP, PTH, and 25-OH-D3 didn't change sig-nificantly before and after treatment ( P>0.05) .Conclusion High-flux dialyser provides a better effect on MBD for patients with diabetic kidney disease undergoing maintenance hemodialysis .

Aim:To characterize the immune response to YCP, a polysaccharide (PS) isolated from marine filamentous fungus Phoma herbarum YS4108. Methods:YCP was coupled to bovine serum albumin (BSA) to construct three neoglycoproteins which are different in their degrees of substitution (DSs; ≈ 3, ≈ 6, or ≈ 10 mol of BSA carrier/mol of YCP hapten, respectively), and their immunogenicities were evaluated in mice following subcutaneous immunization,respectively. IgG subclasses against the PS and the carrier protein were measured in addition to the total IgG and IgM antibodies for YCP induced by the neoglycoproteins. Results: While unconjugated PS was weakly immunogenic, theneoglycoprotein induced vigorous primary IgM and booster IgG responses to PS and the carrier protein. Interestingly,the IgG subclass distribution was different between PS and the carrier protein; for PS, the IgG response was predominant of IgG2a subclass with approximately low levels of IgG2b and IgG1, while the response to the carrier protein was mainly of the IgG1 subclass with relatively low levels of IgG2a and IgG2b, and the lowest of IgG3. Conclusion:YCP has the potential to elicit preferentially IgG2a as the dominant isotype antibody in mice.

Objective To evaluate hepatic perfusion assessed by contrast-enhanced ultrasonography (CEUS) for predicting cirrhosis accurately and non-invasively.Methods Forty patients with cirrhosis and twenty-five healthy controls were given CEUS examination,and time-intensity curves were drawn as the regions of interest located in liver parenchyma by using QLAB analyzing soft.The parameters of the two groups as follows:intensity of arterial perfusion (Iap),intensity of total perfusion of liver parenchyma (Ipeak),intensity of portal venous perfusion (Ipp),the ratio of portal venous perfusion and total perfusion (Ipp/Ipeak) were compared by independent-samples t test,and the diagnostic value of parameters were analyzed by receiver operating characteristic (ROC) curve.Resnlts Iap was bigger,while Ipp,Ipp/Ipeak were smaller in patients than that in controls( P ＜0.001 ).But there was no significant difference on Ipeak between the two groups.When Iap,Ipp,Ipp/Ipeak were used for the diagnosis of cirrhosis,the sensitivity were 67.3 ％,92.7％,96.4％ and the specificity were 80.0％,96.0％,92.3％,respectively.Conclusions CEUS can reflect the changes of the blood perfusion of cirrhotic liver.CEUS parameters Iap,Ipp,Ipp/Ipeak are significant different between the two groups and can be the non-invasive diagosis parameters of cirrhosis.

Objective:To evaluate the performance of paramagnetic particles chemiluminescence microparticle immunoassay ( CMIA) for detection of serum Vitamin B12 ( VitB12 ).Methods: Analysed CMIA system precision, accuracy, anti-interference, analytical measuring range( AMR) ,clinical reportable range ( CRR) and biological reference interval were evaluated,according to the clinical and laboratory standards institude ( CLSI) EP5-A2,EP15-A2,EP7-A2,EP6-A,C28-A3c guidelines.To assess the accuracy,we used the reference material SRM 1955 from national institute of standards and technology ( NIST ) and external quality assessment ( EQA) samples ( LN5-B and K-C) from CAP.Results:The precisions of within-run and between-run were less than standard of manu-facturer when the concentration of VitB12 was 108.84-874.43 pmol/L.The results of SRM1955 met the allowable range of the target val-ue.The results of EQA samples( K-C and LN5-B) also up to the CAP calibration and validation/linear evaluation error limits stipulated standards,and the results through linear verification when the concentration of VitB12 was 89-1 057 pmol/L.The 95% verification interval contains the specified value also.The relative deviation was less than external quality assessment standard from national center for clinical laboratory ( TEa:target value ±25%).Anti-interference evaluation showed without significant interferenc when TG ≤20 mmol/L,Bil ≤300 μmol/L VitC≤1.5 g/L to the VitB12 detection system ( CMIA).AMR validation showed determines the best fit equation was linear equation polynomial.There was the linear relationship when the concentration of VitB12 was 0-1 107 pmol/L.The upper limit of CRR was 110 700 pmol/L,the maximum dilution was 100 times.Biological reference interval validation showed that the overall level of VitB12 in this study reference individuals conform to the standard of manufacturer statement for the population,the overall level of VitB12 in female little higher than male,but no significant differences.Conclusion:Performance of CMIA for detection of serum VitB12 basically met needs of laboratory,which can provide reliable results of VitB12 for laboratory,provide information for the VitB12 status of population in the laboratory evaluation.

To establish a consumables supermarket management system to realize the informatized management of the consumables supermarket. The system used SOA as the main architecture, and IIS manager as the pub-lishing tool. The system had the workload, materials and financial resources decreased while the efficiency in-creased, and made the supervisor master the sale information timely. Consumables supermarket informatized management may decrease the hospital's needs for current capital as well as the links, while increase the hospital effi-ciency greatly.