Objective To investigate the diagnostic value of blood homocysteine,ankle -brachial index and brachial -ankle pulse wave velocity in elderly patients with coronary heart disease (CHD).Methods 97 patients with routine coronary angiography were classified into CHD group (65 cases)and non -CHD group (32 cases) according to the results of coronary angiography.There were 24 cases with single -vessel disease in 65 CHD cases, 21 cases with double -vessel disease and 20 patients with multivessel disease of CHD.Basic clinical parameters,age, gender,TC,TG,LDL -C,HDL -C,etc and blood HCY,ABI,baPWV levels were compared among groups.Results The age of double -vessel disease group,multivessel disease group was significantly higher than that in single -vessel disease group(t =3.721,3.927,all P <0.05).HCY,ABI,baPWV in CHD group were (18.29 ±2.73)μmol/L, (0.97 ±0.16),(16.38 ±1.27)m/s,which had statistically significant differences compared with non -CHD group (HCY:t =5.701,P <0.01;ABI:t =6.138,P <0.01;baPWV:t =15.132,P <0.01 ).There were no significant differences between single -vessel disease group and non -CHD group on the ABI(all P >0.05),and the ABI of multi -vessel disease,double vessel disease group were significantly lower than that of non -CHD group (all P <0.01).HCY,baPWV of CHD group were significantly higher than non -CHD group(all P <0.01 ),double vessel disease,HCY multivessel disease group,ABI,baPWV average water with single -vessel disease group were signifi-cantly different(all P <0.01 ).With the increase of coronary lesions involved,the blood HCY,baPWV showed an increasing trend and ABI showed an decreasing trend.Conclusion Combined detection of HCY,baPWV and ABI has great value in early detection and early intervention of CHD in the elderly.

Objective To investigate the relationship between the resting heart rate (RHR) and target organ damage (TOD) in elderly patients with metabolic syndrome(MS). Methods 264 elderly patients with MS were divided into four groups according to the level of RHR: RHR1 group, RHR<65 beats/minute (bpm) (46 cases) ;RHR2 group, 65≤RHR<75 bpm (77 cases);RHR3 group, 75 bpm≤RHR<85 bpm (89 cases);RHR4 group, RHR≥85 bpm (52 cases).Electrocardiography, echocardiography, carotid uhrasonography, crcatinine clearance rate (Ccr) and quantitative assay of 24 hours' albuminuria were performed. Results (1) Compared with RHR1, RHR2 and RHR3 groups, RHR4 group showed higher levels of carotid intima-medial thickness (IMT), carotid arterial diameter (CAD), left ventricular mass index (LVMI) and albuminuria(P< 0.05 or P<0.01), and lower levels of left ventricular ejection fraction (LVEF) and Ccr (all P< 0.01). (2) The IMT, CAD, LVMI and albuminuria were positively correlated with RHR (r=0.33, 0.23, 0.61, 0.58, respectively, all P<0.01). However, the LVEF and Ccr were negatively correlated with RHR (r=-0.59, -0.51, all P<0.01). (3) Logistic multivariate analysis showed that RHR and pulse pressure (PP) had effects on myocardial hypertrophy, coronary heart disease, heart failure, cerebral stroke and renal dysfunction(P<0.05 or P<0.01). Except heart failure, PP played a more important role than RHR. Coneinsions RHR may be an independent risk factors for TOD in elderly patients with MS,and RHR regulation is important for the development of MS in the elderly.

Objective: To investigate the clinical curative effect of Compound Shajiziyou Suppository (Oleum Hippophae, Fructus Cnidii, Radix Sophorae Flavescentis, Calamina, Resina Olibani, etc.) on cervical erosion. Methods: 212 patients suffering from cervical erosion were treated with 1 piece of Compound Shajiziyou Suppository in vaginal fornix everyday after menstruation, 7 sessions constituting a single therapeutic course. Results: The effective rate during the first therapeutic course was 82.08%. The total effective rate during two therapeutic courses was 94.81%. The curative effect for the mild cervical erosion was better than for the moderate and severe cervical erosion(P

Objective To develop a kind of tissue microarray (TMA) for the teaching of pathological experiment. Methods A series of human normal tissues from heart, lung, liver, stomach, intestine, etc as well as their matched disease tissues were collected and made into TMA parraffin blocks by tissue chip instrument. After section, the slides were stained by hematoxylin-eosin staining and covered by the coverslips which were marked with serial columns and rows. Results All the specimens were arranged in good order on TMA paraffin block and the slides were stained clearly. And the location of each specimen was marked distinctively on TMA slides. Conclusions Compared with classical tissue slides, the TMA slides for the teaching of pathological experiment have many advantages such as good compare, easy memorizing, low expend and simple making. Take together, the TMA for teaching might have a good perspective for application in the future.

[ ABSTRACT] AIM:To investigate the mechanism of L-type calcium channel ( L-Ca2+)/calpain signal transduc-tion pathway in verapamil inversing resistance of papillary thyroid carcinoma to doxorubicin .METHODS:Human papillary thyroid carcinoma TPC-1 cells were cultured for 2 d.For determining the appropriate concentrations and treatment time of verapamil and doxorubicin , a compatibility test was conducted to detect the cell viability by CCK-8 assay.The cells were divided into control group , doxorubicin group , verapamil group and doxorubicin +verapamil group .The techniques of whole-cell patch-clamp was used to record L-Ca2+currents.The protein expression levels of calpain 1 and LC3 were detec-ted by Western blot .RESULTS: Compared with control group , the density of L-Ca2+current decreased in doxorubicin group and verapamil group (P<0.05).Compared with verapamil group , the density of L-Ca2+current decreased in doxo-rubicin+verapamil group (P<0.01).Compared with control group, the expression of calpain 1 decreased in doxorubicin group and verapamil group (P<0.05).Compared with doxorubicin group , the expression of calpain 1 decreased in doxo-rubicin+verapamil group (P<0.05).Compared with control group , the expression of LC3 increased in doxorubicin group and verapamil group (P<0.05).Compared with doxorubicin group , the expression of LC3 increased in doxorubicin +ver-apamil group ( P<0.01) .CONCLUSION:The drug resistance of TPC-1 cells to doxorubicin may be related to the in-crease in autophagic activity .Verapamil further increases autophagic activity of TPC-1 cells, resulting in autophagic death and inversing the resistance of TPC-1 cells to doxorubicin .The mechanism may be involved in L-Ca2+/calpain 1 signal transduction pathway of autophagy .

OBJECTIVETo explore the source and morphology of supernumerary markers from patients with 47,XYY/47,XY, +mar and supermale syndrome.

METHODSConventional GTG banded karyotyping and dual-color fluorescence in situ hybridization (FISH) were performed on 21 such patients.

RESULTSAmong these cases, 18 had their small supernumerary marker derived from the Y chromosome. Three were derived from autosomal chromosomes. Those derived from Y chromosome were small fragments with centromeres, while those derived from autosomes were in the ring form.

CONCLUSIONIn children with supermale syndrome and 47,XYY/47,XY,+ mar, the supernumerary marker chromosomes primarily derive from sex chromosomes. These small chromosomes mainly have the forms of small segments with centromeres or rings. For such children, molecular cytogenetic analysis can facilitate genetic counseling and prenatal diagnosis.

Adolescent ; Child ; Child, Preschool ; Chromosome Aberrations ; Chromosome Banding ; Humans ; In Situ Hybridization, Fluorescence ; Infant ; Male ; Sex Chromosome Disorders ; genetics ; XYY Karyotype ; genetics

Objective To investigate the awareness of self protection and practice of nurses from urology department when giving intravesical chemotherapy to patients with urinary bladder cancer.Methods One survey via a self-designed questionnaire and an on-the-spot examination of anticancer drugs preparing were done and compared among 42 nurses from urology department and 48 nurses from oncology department.Results All nurses from oncology department were trained with anticancer drugs-associated knowledge and 83.3％ nurses from urology department had received some training (P＜0.05).Over 64.6％ nurses from department of medical oncology were familiar with all the five parts of chemotherapy drugs protection protocol,while only 40.5％ nurses from department of urology were aware of them.During the on-the-spot examination,50.0％ and 21.4％ nurses respectively from oncology department and urology department could complete seven anticancer drugs preparation procedure correctly (P＜0.05).Conclusions The nurses from urology department had weak awareness of self protection and were lack of chemotherapy associated training and standard practice when giving intravesical chemotherapy.It is imperative to work out a protection plan to educate the nurses and establish the protocols for preparing anticancer drugs to reduce the occurrence of occupational hazard.

Objective Investigation in tertiary hospital clinical laboratory in pursuance of the current state of the sharp injury incidence and influencing factors,analyze the causes and discuss the control strategy.Methods Application of cross-sectional survey method,according to the unified investigation questionnaire investigation,with chi-square test and the multi-factor Logistic regression analysis methods to analyze research data.Results The work environment exit contact with injection needles,glass and other sharps,contact with noise,contact with chemicals,contact the patient's blood and other biological specimens,high-risk groups,the patient and family to negative emotions,by patients and their families complain or abuse related influencing factors.In the organization and management factors,in order to work strength is insufficient for the most dissatisfied,protective equipment,medical staff personal cognitive factors of occupational exposure,failing to abide by the medical care standard operation and high incidence after exposure to failed to report.Somewhere in tertiary hospital clinical laboratory in pursuance of the incidence of sharps injuries associated with five factors:different jobs,working strength,protective equipment perfect or not,sharp instrument use and disposal of medical wastes.Conclusion Tertiary hospital clinical laboratory in pursuance of the incidence of sharp injury related to many factors,Hospitals should formulate corresponding rules and regulations,improve the working environment,the standard safety operation,reduced the incidence of sharps injuries.

Objective To investigate the awareness of self protection and practice of nurses from urology department when giving intravesical chemotherapy to patients with urinary bladder cancer.Methods One survey via a self-designed questionnaire and an on-the-spot examination of anticancer drugs preparing were done and compared among 42 nurses from urology department and 48 nurses from oncology department.Results All nurses from oncology department were trained with anticancer drugs-associated knowledge and 83.3％ nurses from urology department had received some training (P＜0.05).Over 64.6％ nurses from department of medical oncology were familiar with all the five parts of chemotherapy drugs protection protocol,while only 40.5％ nurses from department of urology were aware of them.During the on-the-spot examination,50.0％ and 21.4％ nurses respectively from oncology department and urology department could complete seven anticancer drugs preparation procedure correctly (P＜0.05).Conclusions The nurses from urology department had weak awareness of self protection and were lack of chemotherapy associated training and standard practice when giving intravesical chemotherapy.It is imperative to work out a protection plan to educate the nurses and establish the protocols for preparing anticancer drugs to reduce the occurrence of occupational hazard.

Objective Investigation in tertiary hospital clinical laboratory in pursuance of the current state of the sharp injury incidence and influencing factors,analyze the causes and discuss the control strategy.Methods Application of cross-sectional survey method,according to the unified investigation questionnaire investigation,with chi-square test and the multi-factor Logistic regression analysis methods to analyze research data.Results The work environment exit contact with injection needles,glass and other sharps,contact with noise,contact with chemicals,contact the patient's blood and other biological specimens,high-risk groups,the patient and family to negative emotions,by patients and their families complain or abuse related influencing factors.In the organization and management factors,in order to work strength is insufficient for the most dissatisfied,protective equipment,medical staff personal cognitive factors of occupational exposure,failing to abide by the medical care standard operation and high incidence after exposure to failed to report.Somewhere in tertiary hospital clinical laboratory in pursuance of the incidence of sharps injuries associated with five factors:different jobs,working strength,protective equipment perfect or not,sharp instrument use and disposal of medical wastes.Conclusion Tertiary hospital clinical laboratory in pursuance of the incidence of sharp injury related to many factors,Hospitals should formulate corresponding rules and regulations,improve the working environment,the standard safety operation,reduced the incidence of sharps injuries.