Objective To explore the clinical effect of blood perfusion combined with penehyclidine hydrochloride injection in the treatment of acute severe organophosphorus pesticide poisoning.Methods 58 patients with severe organophosphorus pesticide poisoning were selected as the research subjects.By using digital table method,they were randomly divided into observation group and control group,29 cases in each group.The two groups were given conventional treatment and blood perfusion treatment after admission,the observation group was treated with penehyclidine hydrochloride injection on this base.Before and after treatment,the myocardial enzymes,liver,kidney function index,blood gas analysis,cholinesterase activity,as well as other treatment of the two groups were observed,and the results were compared.Results Compared with the control group,after treatment,the myocardial enzyme,transaminase,serum amylase and creatinine levels in the observation group were significantly lower,cholinesterase level was significantly increased,the oxygen partial pressure increased,the partial pressure of carbon dioxide decreased obviously,the differences were statistically significant(all P<0.05).The hospitalization time and cholinesterase activity recovery time of the observation group were significantly shorter than those of the control group[(12.57±2.14)d vs.(16.35±2.73)d,(5.46±2.29)d vs.(7.61±3.47)d],and the cure rate of the observation group was significantly higher than that of the control group(100.00% vs.82.76%),the differences were statistically significant(t=2.139,2.208,x2=5.472,all P<0.05).Conclusion Blood perfusion can directly adsorb organophosphorus pesticide,shorten the recovery time of cholinesterase and reduce the case fatality rate,and it combined with penehyclidine hydrochloride injection not only can improve the clinical curative effect of blood perfusion,but also can protect the organ.

The purposes of this study were: (1) to test healthy young adults' ankle plantar and dorsal flexor muscles strength, providing integrate data and scientific basis for sports training, sports injury and recovery. (2) to investigate the biomechanical rule of healthy adults' ankle plantar and dorsal flexor muscles. Methods: 70 college students (35males and 35 females) volunteerily took part in the test. An advanced Cybex-6000 dynamometer was employed. This test consisted of two sections, isokinetic concentric (CON) test and isometric test. Results: males and females adults' ankle dorsal flexor isometric peak torque (PT) increased and plantar flexor isometric PT decreased with the increasing of testing angle. CON PT decreased while the test angle velocities increased. Plantar flexor PT were higher than corresponding dorsal flexor in two tests ( P

Objective To investigate the clinical value of serum cardiac troponin Ⅰ(cTNⅠ) in diagnosis of cardiac injury in neonatal hypoxic ischemic encephalopathy(HIE).Methods 20 healthy newborns were selected as the control group.58 patients with HIE were divided into mild group(n=20),medium group(n=19) and the severe group(n=19),respectively.The level of cTNⅠ was detected by immunofluorescence.Results Serum levels of cTNⅠ in experimental group were obviously higher than that of the control group;There were significant difference between any two groups(P

Objective To study the manifestations and electroencephalography(EEG) features of benign childhood occipital epilepsy in order to improve clinical diagnosis.Methods The clinical and EEG characteristics of 26 patients diagnosed as benign childhood occipital epilepsy were retrospectively reviewed.Results The clinical characteristics were as follow:(1)Visual symptoms.(2)Motor symptoms:versive seizure tonic clonic seizures.(3)Automatism EEG features:discharge over one both occipital lobe.Conclusion Benign childhood occipital is a distinctive epilepsy syndrome.Understanding the clinical manifestations and EEG features can lead to obtain correct diagnosis.

Objective To investigate the MRI characteristics of periventricular leukomalacia in children. Methods The clinical datas of 39 cases of PVL were retrospectively analyzed.Results MRI manifestation:(1) quantitative reduction of white matter:only in trigonal area 12 cases;at trigonal and semiovale center 7 cases.(2)Fo- ci form:patchy areas in 20 cases;punctate areas 13 cases;streake in 6 cases.(3)The signal of the focus:hyperin- tense/intense on T1 weighted and hyperintense on T2 weighted.(4)Ventricular enlarged:body of lateral ventricle 31 cases,triangular part 8 cases.(5)Ventricular appearance irregular 11 cases.(6)Others:corpus callosum dysplasia 2 cases.4 cases bilateral lateral fissure deepened and widened.Conclusion MRI is a way to diagnose PVL in early, level and scope of focus of PVL can be clearly displayed by MRI.

The commonest cause of death in patients after liver surgery is liver failure,especially in patients with cirrhosis.A proper preoperative assessment of liver function can significantly impact on the rate of postoperative liver failure. Similarly, a good postoperative assessment of liver function can help to predict recovery of liver function and to guide treatments,especially in the surgical ICU.There are more and more methods which can be used to assess liver function,such as liver biochemical indicators,comprehensive scoring systems,quantitative liver function tests and liver volumetric measurements.From the modern medical aspect,using the evalution method for liver function which has mentioned before often exist great bias. The combined use of several methods,which can give more accurate assessment of liver function,is recommended.

Objective To evaluate the etiology and manifestation of computerized tomography(CT) with lacunar infarction in children. Methods Forty-three cases with lacunar infarction were studied.Results The casuses included minor head injury(21 cases),upper respiratory infection(8 cases),varicella(2 cases),mitochondrial encephalomyopathy(1 case),moyamoya diseses(1 case),systemic lupus erythematosus(1 case) and unknown causes(9 cases).The clinical manifestations included hemiparalysis(41 cases),aphasia(5 cases),epilepsy(10 cases),sudden deafness(2 cases).A total of 51 focal ischemic lesions were found with CT in 43 patients.CT scan in 15 cases showed punctuate calcification focus with circular infarcts of low density beside them in single or bilateral basal ganglia.The clinical symptoms in 40 cases disappeared within 12 weeks after therapy.Conclusions The review indicates that children with lacunar infarction have a favorable prognosis,which is probably a result of the difference in lacunar infarction etiology between children and adults.Basal ganglia calcification is somewhat association with acute hemiplegia in infants minor head trauma sustained.

The concrete methods and experiences for foreign students in case study in the past decades were introduced in this article. We emphasized on teaching students in accordance with their aptitude and step by step, explaining the profound things in a simple way and giving attention to different students' capabilities during teaching. In the practice, we surely felt that case study will bring more advantage than traditional teaching methods, motivate students and improve the capabilities to understand the course content.

Objective To investigate the sedative effects of different fractions of patrinia scabiosaefolia fisch. in mice and provide some valuable information for isolating components with sedative effect.Methods Petroleum, acetic ether and n-butanol fraction was isolated from the patrinia scabiosaefolia fisch.. The sedative effects were determined with locomotor activity assay, hole board test and sodium thiopental-induced sleeping time assay, and it was compared with the effects of total 70% ethanol extract. Results N-butanol fraction showed better sedative effect compared with others, and it was in a dose-dependent manner. Conclusion The results showed that the main active principles of patrinia scabiosaefolia fisch. were the n-butanol fraction, and the promising active chemical might be obtained from this fraction.

Objective To investigate the effects of hyaluronan (HA) and CD44 on airway mucous hypersecretion,and explore the molecular mechanism of activation of epidermal growth factor(EGF)/epidermal growth factor receptor(EGFR) signal transduction pathway by signal factors. Methods BEAS-2 B airway epithelial cells were cultured in vitro,and were stimulated by neutrophil elastase(NE).Reactive oxygen species(ROS)scavenger(DMTU),hyaluronidase(Hase),CD44 antibody and tissue kallikrein(TK)inhibitor(PI)were served as interventional factors,and control group(serum free culture),NE stimulation group(50 nmol/L NE),DMTU+NE group(20μmol/L DMTU+50 nmol/L NE),DMTU+Hase+NE group(20 μmol/L DMTU+10μg/mL Hase+50 nmol/L NE),CD44Ab+NE group(5 μg/mL CD44Ab+50 nmol/LNE)and PI+NE group(1 00μg/mL PI+50 nmol/L NE)were established.The expression of mucin(MUC)5AC mRNA was detected by RT-PCR,the expression of MUC5AC and EGF protein was determined by ELISA,and the expression of phosphorylated epidermal growth factor receptor(p-EGFR)protein was analysed by Western blotting. Results The expression of MUC5AC,EGF and p-EGFR protein and MUC5AC mRNA in NE stimulation group was significantly higher than that in control group(P<0.01),the expression in DMTU+NE group was significantly lower than that in NE stimulation group(P<0.01),the expression in MTU+Hase+NE group was significantly higher than that in DMTU+NE group(P<0.05 for MUC5AC and p-EGFR protein and MUC5AC mRNA,and P<0.01 for EGF protein),the expression in CD44Ab+NE group and PI+NE group was significantly higher than that in NE stimulation group(P<0.05 for MUC5AC and p-EGFR protein and MUC5AC mRNA,and P<0.01 for EGF protein). Conclusion NE up-regulates the expression of MUC5AC gene via ROS/HA/CD44/TK/EGF/EGFR signal transduction pathway in airway epithelial cells.