This article reviewed the application of Regent(fipronil),emphasizing its current research advance in the degradation in environment and toxicity of Regent(fipronil) and its metabolite,and its degradation action.The main degradation approach is photolysis and hydrolysis in water,and simultaneously occurring oxidation in the soil.Fipronil is toxic for bee,freshwater vertebrate and birds,but the effect of fipronil on human being should be researched further.More attention should be paid to the safety for human in application of fipronil.

Objective The complications of internal fication for calcaneal fracture were summarized and discussed to increase awareness of these complications, and to improve decision making and efficacy of the treatment. Methods The patients admitted to our hospital from October 1997 to June 2003 were followed up. The fracture in 68 feet (66 patients) was treated with open reduction and internal fixation (ORIF) using steel plate. The complications associated with ORIF were analyzed and discussed as to etiology, incidence, diagnosis, prevention and treatment. Results The patients were followed up for 7 to 57 months. Complications were found in 14 cases, including postoperative wound dehiscence in 5 (infection in 2), malunion (failure to achieve reduction) and arthritis in 9, accompanied by nerve injury in 6. The percentage of complication was 20 6%. With timely treatment, good function was regained in most of the patients. Conclusion Should attention be paid to the operation, many complications could be avoided. ORIF with plate is one ot the options for the treatment of this fracture, but the operative technic and the device should be improved. As the anatomy of the calcaneus is different from a long bone, it is unsuitable to apply the principle and device that are used in the internal fixation of a long bone to this fracture. The plate and screws used in the fixation of the calcaneal fracture can not give enough support

OBJECTIVE:To study the genetic diversity of germplasm resources of Duchesnea indica from Hunan. METHODS:The random amplified polymorphic DNA(RAPD)reaction system of germplasm resources was established and RAPD-PCR was ad-opted to detect the expressions of 24 groups of D. indica from Hunan(including 21 wild and 3 cultivated varietos in different geo-graphical distribution). Agarose gel electrophoresis was used to analyze the results and calculate allele number (Na),effective al-lele number (Ne) the polymorphism points percentage (PPB),Nei’s gene diversity index (H) and Shannon’s information index (I). RESULTS:Totally 14 polymorphic primers were screened and 90 electrophoresis bands were amplified,including 81 polymor-phic bands with Na of 1.900 0,Ne of 1.540 1,PPB of 90.0%,H of 0.312 8 and I of 0.467 5. CONCLUSIONS:The genetic diver-sity of D. indica from Hunan is rich,and there were differences in genetic background between the cultivated species and wild spe-cies;clustering results show significant correlation with geographic distribution.

ObjectiveBased on the conjoint analysis of transcriptome and metabolome， the key genes in the phenylpropanoid biosynthesis pathway of Lonicera macranthoides were explored， which provided a basis for further exploring the synthesis and regulation mechanism of phenylpropanoid compounds in "Xianglei" L. macranthoides. MethodsThe stem， leaves， and three flowering flowers of "Xianglei" L. macranthoides were selected as experimental materials to construct transcriptome and metabolome. The transcriptome and metabolomics were conjointly analyzed by the Kyoto Encyclopedia of Genes and Genomes （KEGG） database and weighted correlation network analysis （WGCNA）， and the key genes in the phenylpropanoid biosynthesis pathway of L. macranthoides were explored. ResultsIn this study， 77 differential phenylpropanoids and 315 differential genes were found. Through the joint analysis of transcription and metabolism， nine key differential metabolites and four key genes related to them were finally discovered. Among them， cinnamic acid， 5-O-caffeoylshikimic acid，sinapyl alcohol， and chlorogenic acid were higher in flowers， and the content of the iconic effective component， namely chlorogenic acid，decreased sharply during the withering period. Caffeic acid，ferulic acid， 5-hydroxyconiferaldehyde，p-coumaryl alcohol， and syringin were higher in leaves. These four key genes belong to the cinnamic alcohol dehydrogenase （CAD） family， 4-coumaric acid： Coenzyme A （4CL） family， hydroxycinnamyl transferase （HCT） family， and L-phenylalanine ammonlyase （PAL） family genes. ConclusionAmong the four key genes excavated from L. macranthoides， TRINITY_DN42767_c0_g6 is related to the synthesis of p-coumaryl alcohol and sinapyl alcohol. TRINITY_DN43525_c4_g1 uses caffeic acid，ferulic acid，and cinnamic acid as substrates to catalyze the next reaction. TRINITY_DN47958_c3_g4 correlates with the synthesis of 3-p-coumaroyl quinic acid and caffeoyl-CoA， and TRINITY_DN52595_c1_g2 correlates with cinnamic acid synthesis. These findings provide a basis for further exploring the synthesis and regulation mechanism of phenylpropanoids in "Xianglei" L. macranthoides.