Objective To investigate the application of a new type internal anastomotic appliance of pouch pliers in the retention of anus operation of mesal,low rectal carcinoma.Methods The data of 65 patients with mesal,low rectal carcinoma with the technique of the anal retention using KYGW type anastomotic appliance of pouch pliers were reviewed and analyzed retrospectively.Results 65 patients were coincided success only one time. Anastomotic fistula occurred after operation in 1 case,being cured and discharged at last,no anastomotic stricture. Conclusion The internal anastomotic appliance of pouch pliers in the anal retention of mesal-low rectal carcinoma is an efficient method with better cost-effectiveness and fewer complications,which is easy to manipulate and popular- ize.

Magnetotactic bacteria can orient and migrate along ambient geomagnetic field lines because of their intracellular magnetic particles ( referred as magnetosomes) , which comprise nanometer-sized, membrane-bound crystals of the magnetic iron minerals. Magnetosome formation is a mineralization process with very strict biological controls over the accumulation, transportation and nucleation in the cell. This paper describes the current progresses of magnetosome formation and the function of proteins involved in this biomineralization process.

Objective To investigate behavior,knowledge and attitude of cigarette smoking,and their relationship among different occupational population in Jiangsu Province.Methods Convenient sampling was used to interview 516 staff from medical institution,schools,government offices,public transportations and waiting rooms,restaurants and internet bars.The content of interview included current smoking,second-hand smoking,and related knowledge and attitude.Results Proportion of current smoking in male(51.2%)was higher than that in female(0.3%,P90%),while it's relatively lower in "second-hand smoking and exact related diseases"(

Objective To investigate the effects of heart displacement on hemodynamics during off-pump coronary artery bypass grafting (OP-CABG) while the sites for anastomosis were being exposed. Methods Forty-seven patients of both sexes (36 male, 11 female) aged 50-82 years undergoing OP-CABG were enrolled in the study. Preoperative cardiac function was assessed : class Ⅱ in 22 patients; Ⅲ in 23 and Ⅳ in 2 according to NYHA classification.The mean ejection fraction was 0.55?0.14 before surgery.They received on average 3.2 grafts. Premedication consisted of intramuscular morphine 10 mg, midazolam 3-5 mg and scopolamine 0.3 mg.Before induction of anesthesia ECG and SpO2 were monitored and radial artery was cannulated for continuous direct BP monitoring. Anesthesia was induced with midazolam 0.1 nig?kg-1 , fentanyl 4?g?kg-1 and pancuronium 0.1 mg?g-1 iv.The patients were mechanically ventilated after tracheal intubation and PETCO2 was maintained at about 40 mm Hg. Anesthesia was maintained with isoflurane and 50%-60% N2O in O2 and intermittent intravenous boluses of fentanyl and pancuronium. Swan-Ganz catheter which can continuously monitor mixed venous blood O2 saturation (SvO2) was placed in pulmonary artery via right internal jugular vein. SvO2, cardiac output (CO), BP, pulmonary arterial pressure (PAP) and HR were continuously monitored. Right atrial pressure (RAP) and PAWP were measured intermittently. Cardiac index (CI),stroke index (SI),systemic vascular resistance index (SVRI),PVRI, left and right ventricular work index (LVWI,RVWI) and left and right ventricular stroke work index (LVSWI,RVSWI) were calculated. The hemodynamic parameters were recorded after induction of anesthesia before surgery (T1,baseline),before heart displacement (T2), while anastomosis to anterior descending branch was being made (T3), while anastonosis to right coronary artery or posterior descending branch (T4) and to left circumflex artery or diagonal branch (T5) was being made, after normal heart position was resumed (T6) and at the end of operation (T7). Results While anastomosis to the anterior descending branch was being made (T3) SI and LVSWI significantly decreased as compared with the baseline (P

This study is designed to obtain recombinant human acetylcholinesterase (rhAChE) and apply it in screening acetylcholinesterase inhibitors. The rhAChE was overexpressed in HEK293 cells transfected by plasmid of pCMV-AChE with the cationic liposome and rhAChE was found to be secreted into cell culture medium. AChE activity was assayed according to modified Ellman method to obtain kinetic parameters. IC so50 values for donepezil compounds of rhAChE were calculated to determine their activities of inhibition. The results showed that Km value was 151.9 micromol.L-1 donepezil inhibited rhAChE in a mixed competitive-noncompetitive way (Ki= 16.03 nmol.L-1, Ki = 18.36 nmol.L-1) and that most new compounds tested exhibited high activities of inhibition on rhAChE. The study suggests that rhAChE is available to be applied in screening AChE inhibitors in vitro.
Acetylcholinesterase ; genetics ; metabolism ; Cholinesterase Inhibitors ; analysis ; pharmacology ; HEK293 Cells ; Humans ; Indans ; analysis ; pharmacology ; Inhibitory Concentration 50 ; Kinetics ; Piperidines ; analysis ; pharmacology ; Plasmids ; Recombinant Proteins ; genetics ; metabolism ; Transfection

12E7 Antigen ; Antigens, CD ; metabolism ; Cell Adhesion Molecules ; metabolism ; Child, Preschool ; Chondrosarcoma, Mesenchymal ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Male ; Neuroblastoma ; metabolism ; pathology ; Sarcoma, Ewing ; metabolism ; pathology ; Spinal Cord Neoplasms ; metabolism ; pathology ; surgery ; Spinal Neoplasms ; metabolism ; pathology ; Thoracic Vertebrae ; Vimentin ; metabolism

OBJECTIVETo evaluate the therapeutic effect of teeth with large periapical lesions followed apical surgery.

METHODS59 patients (59 periapical lesions, 91 teeth) with large periapical lesions ranging in size from 6 mm to 21 mm in diameter were included in this study. All teeth were treated by root canal therapy and apical surgery, and the surgical procedure was completed with microsurgical technology and ultrasonic root-end preparation. The outcomes were evaluated clinically and radiographically at 1, 3, 6, 9, 12 and 24-month intervals, and the various factors which may affect the success rate were analyzed statistically.

RESULTS52 cases (88.1%) of 59 patients were considered success, 6 cases (10.2%) were considered failure, and 1 case (1.7%) was considered in progress. The success rate of elderly patients group (63.6%) was significantly lower than that of the youth group (87.5%) and middle-aged group (95.0%) (P = 0.037; P = 0.017). The success rate of molar group (75.0%) was significantly lower than that of the anterior group (92.6%) and the premolar group (90.0%) (P = 0.041, P = 0.047); however, there was no significant difference in the gender, the number of teeth invaded in lesions, the size of periapical lesion (P > 0.05).

CONCLUSIONIt is an effective therapy for the teeth with large periapical lesions by apical surgery after root canal treatment.

Adolescent ; Aged ; Bicuspid ; Humans ; Middle Aged ; Molar ; Root Canal Filling Materials ; Root Canal Therapy ; Tooth Root

In China, rural doctors and primary health institutions take important responsibilities as the basic of public health system.They play the fundamental role in the construction of public health system in the rural area. However, primary public health is facing the challenge of poor infrastructure, weak human resources, and inefficient services in most areas. We employed field investigation and literature review to identify major problems on the implementation of primary health service and the development of rural doctors' team. The recommendation in this study might provide scientific evidence to improve the construction of public health system and policy-making in rural area in China.

OBJECTIVETo investigate the effects of decompresion through double-incision of foot dorsum on the treatment of osteofascial compartment syndrome of the foot under the monitoring of saturation of blood oxygen.

METHODSFrom January 2000 to June 2007, 26 cases of osteofascial compartment syndrome of the foot were decompressed through double-incision of foot dorsum under the monitoring of saturation of blood oxygen, and relaxation suture or skin grafting were operated within 3 to 10 days after decompressing. Among them, 22 patients were males and 4 were females, with an average age of 36.3 years old ranging from 22 to 68 years. According to AOFAS system, the pain, function, autonomic activities and support were evaluated.

RESULTSAll patients were followed-up for from 6 to 43 months with the average of 19 months. All patients were healed. According to AOFAS system, the total scores increased from preoperative (30.4 +/- 8.02) to postoperative (92.5 +/- 5.0) (t = 3.13, P < 0.01); the results were excellent in 21 cases, good in 4 and poor in 1.

CONCLUSIONThe patients of fracture-dislocated, swelling and injured in the soft tissue because of severe violence should observed closely on osteofascial compartment syndrome of the foot early. Feet are operated and thoroughly decompressed as soon as it is diagnosed as the compartment syndrome. Osteofascial compartment syndrome of the foot decompressed by foot dorsum double-incision is convenient and satisfied, and the operation is performed by internal fixation if it is displacedly fractured at the same time.

Adult ; Aged ; Compartment Syndromes ; blood ; diagnosis ; physiopathology ; surgery ; Early Diagnosis ; Female ; Foot ; blood supply ; physiopathology ; surgery ; Humans ; Male ; Middle Aged ; Oxygen ; blood ; Young Adult

OBJECTIVETo investigate the clinicopathologic features of reactive hyperplasia of lymph nodes in maxillofacial regions.

METHODSThirty-two patients with lymph node reactive hyperplasia (LRH) were analyzed clinicopathologically including pathomorphologic manifestation and immunohistochemical expression.

RESULTSHistopathology of 32 patients showed significant hyperplasia in folliculus lymphaticus, marginal zone lymph, paracortical area lymph, and seldom mixed hyperplasia. Immunohistochemical study showed that the hyperplastic lymphocytes were mainly composed of CD20 positive B cells positive in 23 cases(72%), CD3 positive T cell in 7 cases (22%), CD20 positive T cell and CD3 positive B cell partially as well as histiocytes in 2 cases (6%). Atypical hyperplasia was found in 10 cases.

CONCLUSIONHyperplastic lymphocytes in LRH of maxillofacial regions are mainly composed of B cells. Malignant transformation may occur in these patients with atypical hyperplasia. Regularity follow up is necessary for these patients.

Adult ; B-Lymphocytes ; Female ; Humans ; Hyperplasia ; Lymph Nodes