1.Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid.
Jin Fu PENG ; Li LIU ; Cheng Xian GUO ; Shi Kun LIU ; Xiao Ping CHEN ; Li Hua HUANG ; Hong XIANG ; Zhi Jun HUANG ; Hong YUAN ; Guo Ping YANG
Biomolecules & Therapeutics 2015;23(5):400-406
MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.
Computational Biology
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Fatty Acids, Nonesterified
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Humans
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Liver
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Luciferases
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MicroRNAs
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RNA, Untranslated
2.Increased Serum Cathepsin K in Patients with Coronary Artery Disease.
Xiang LI ; Yuzi LI ; Jiyong JIN ; Dehao JIN ; Lan CUI ; Xiangshan LI ; Yanna REI ; Haiying JIANG ; Guangxian ZHAO ; Guang YANG ; Enbo ZHU ; Yongshan NAN ; Xianwu CHENG
Yonsei Medical Journal 2014;55(4):912-919
PURPOSE: Cathepsin K is a potent collagenase implicated in human and animal atherosclerosis-based vascular remodeling. This study examined the hypothesis that serum CatK is associated with the prevalence of coronary artery disease (CAD). MATERIALS AND METHODS: Between January 2011 and December 2012, 256 consecutive subjects were enrolled from among patients who underwent coronary angiography and percutaneous coronary intervention treatment. A total of 129 age-matched subjects served as controls. RESULTS: The subjects' serum cathepsin K and high sensitive C-reactive protein (hs-CRP) and high-density lipoprotein cholesterol were measured. The patients with CAD had significantly higher serum cathepsin K levels compared to the controls (130.8+/-25.5 ng/mL vs. 86.9+/-25.5 ng/mL, p<0.001), and the patients with acute coronary syndrome had significantly higher serum cathepsin K levels compared to those with stable angina pectoris (137.1+/-26.9 ng/mL vs. 102.6+/-12.9 ng/mL, p<0.001). A linear regression analysis showed that overall, the cathepsin K levels were inversely correlated with the high-density lipoprotein levels (r=-0.29, p<0.01) and positively with hs-CRP levels (r=0.32, p<0.01). Multiple logistic regression analyses shows that cathepsin K levels were independent predictors of CAD (odds ratio, 1.76; 95% confidence interval, 1.12 to 1.56; p<0.01). CONCLUSION: These data indicated that elevated levels of cathepsin K are closely associated with the presence of CAD and that circulating cathepsin K serves a useful biomarker for CAD.
Aged
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Biological Markers/blood
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C-Reactive Protein/metabolism
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Cathepsin K/*blood
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Coronary Artery Disease/*blood/metabolism
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Female
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Humans
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Male
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Middle Aged
3.Increased Serum Cathepsin K in Patients with Coronary Artery Disease.
Xiang LI ; Yuzi LI ; Jiyong JIN ; Dehao JIN ; Lan CUI ; Xiangshan LI ; Yanna REI ; Haiying JIANG ; Guangxian ZHAO ; Guang YANG ; Enbo ZHU ; Yongshan NAN ; Xianwu CHENG
Yonsei Medical Journal 2014;55(4):912-919
PURPOSE: Cathepsin K is a potent collagenase implicated in human and animal atherosclerosis-based vascular remodeling. This study examined the hypothesis that serum CatK is associated with the prevalence of coronary artery disease (CAD). MATERIALS AND METHODS: Between January 2011 and December 2012, 256 consecutive subjects were enrolled from among patients who underwent coronary angiography and percutaneous coronary intervention treatment. A total of 129 age-matched subjects served as controls. RESULTS: The subjects' serum cathepsin K and high sensitive C-reactive protein (hs-CRP) and high-density lipoprotein cholesterol were measured. The patients with CAD had significantly higher serum cathepsin K levels compared to the controls (130.8+/-25.5 ng/mL vs. 86.9+/-25.5 ng/mL, p<0.001), and the patients with acute coronary syndrome had significantly higher serum cathepsin K levels compared to those with stable angina pectoris (137.1+/-26.9 ng/mL vs. 102.6+/-12.9 ng/mL, p<0.001). A linear regression analysis showed that overall, the cathepsin K levels were inversely correlated with the high-density lipoprotein levels (r=-0.29, p<0.01) and positively with hs-CRP levels (r=0.32, p<0.01). Multiple logistic regression analyses shows that cathepsin K levels were independent predictors of CAD (odds ratio, 1.76; 95% confidence interval, 1.12 to 1.56; p<0.01). CONCLUSION: These data indicated that elevated levels of cathepsin K are closely associated with the presence of CAD and that circulating cathepsin K serves a useful biomarker for CAD.
Aged
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Biological Markers/blood
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C-Reactive Protein/metabolism
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Cathepsin K/*blood
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Coronary Artery Disease/*blood/metabolism
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Female
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Humans
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Male
;
Middle Aged