1.Clinical study of off-pump placation for left ventricular aneurysm
Hart-Song SUN ; Xiang-Ming FAN ;
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(03):-
Objective To evaluate the surgical results of off-pump for plication for left ventricular aneurysm. Methods The study included 34 patients with left ventricular aneurysm,who underwent left ventricular aneurysmecto- my and plicatien on pump in group Ⅰ(17 cases)and simply aneurysm plicatien off pump in group Ⅱ(17 cases).All patients except two in group I underwent coronary artery bypass grafting simultaneously.Results There was one early death in group Ⅱ because of refractory ventricular arrhythmia.The complication included atrial fibrillation,ventricular arrhythmias,heart failure and delayed healing of wound.The aneurysm in group Ⅰ[(45?15)%of the ventricle]is larger than in groupⅡ[(29?12)%of the ventricle,P
2.Molecular action mechanism of desoxyrhaponticin and serum albumin characterized by spectroscopy combined with molecular modelling.
Ming GUO ; Wen-Xiang FAN ; Run-Huai HU
China Journal of Chinese Materia Medica 2014;39(6):1075-1082
OBJECTIVETo study the molecular action mechanism of active constituents desoxyrhaponticin (DES) and human serum albumin (HSA).
METHODUnder the simulated physiological condition, computer analog technology, fluorescent spectrometry and ultraviolet spectrum were combined to study the binding mechanism between drug and protein.
RESULTMolecular modeling was adopted to establish the binding model between DES and HSA, suggesting that the interaction force maintaining drug and protein is mainly the hydrophobic interaction with a hydrogen-bond interaction. The results from spectroscopy indicated that the interaction between DES and HSA is a dynamic binding process with a high intensity. The value of the binding distance (r) between DES and HSA was low, which demonstrate the occurrence of energy transfer. DES made an impact on HSA' structural domain microcell conformation, which resulted in hydrophobic changes in binding areas. According to the fluorescent phase diagram technical analysis, the changes in the DES-HSA reaction conformational pattern showed a "two-state" model. According to the obtained thermodynamic parameters for the DES-HSA interaction, the interactional force between DES and HSA was mainly a hydrophobic interaction. The fluorescence polarization proved that a non-covalent compound was generated during the interaction between DES and HSA.
CONCLUSIONThe spectrum experiment showed consistent results with the computer analog technology, which could provided certain reference for studies on the interaction between DES and HSA.
Humans ; Models, Molecular ; Protein Binding ; Protein Conformation ; Serum Albumin ; chemistry ; metabolism ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet ; Stilbenes ; metabolism ; Thermodynamics
3.Improving doctor-patient relationship, enhancing the oral cavity medical service effect
Ming-cong YANG ; Xiao-ping FAN ; Xue-rong XIANG
Chinese Journal of Medical Education Research 2011;10(7):851-853
While the medicine pattern of biomedicine turn to biological-psychology-society, the medical trouble communication becomes more and more important in the medical service. Good medical trouble communication ability is the essential condition of doctor. As oral cavity clinicians, only by gasping the principle of communication can we appropriately utilize some skills of communication exchange,establish the good medical trouble relations with the patient and achieve the good treatment result finally.
4.Studies on biomechanics of human foot: a review.
Xiang DONG ; Yubo FAN ; Ming ZHANG ; Junkai CHEN
Journal of Biomedical Engineering 2002;19(1):148-153
The plantar pressure and shear force measurement, the function and effect of joints, bones and ligaments of foot during running and walking, and the modeling and finite element analysis of human foot are three important aspects in biomechanical study of foot. They are also the basis of the study of rehabilitation. In this paper, studies on the three aspects are reviewed, and further promising developments are proposed.
Biomechanical Phenomena
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Diabetic Foot
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physiopathology
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Exercise
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physiology
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Finite Element Analysis
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Foot
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physiology
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Humans
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Pressure
5.Study on inhibitory effects and mechanism of lipophilic components in Salvia miltiorrhiza on angiogenesis in vitro.
Xue-Mei FAN ; Gui-Xiang REN ; Qiong-Lin LIANG ; Yi-Ming WANG ; Guo-An LUO
China Journal of Chinese Materia Medica 2014;39(4):744-747
In this study, the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis, and explore its possible mechanism. The cell model was established to determine the effect of lipophilic components in S. miltiorrhiza on the proliferative activity and migration capacity of endothelial cells. Then the realtime fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cytokines VEGF-A, VEGF-C and MMP-9. The results showed that 5 mg x L(-1) lipophilic components in S. miltiorrhiza could inhibit the proliferation and migration of endothelial cells, and reduce the expression of VEGF-A and MMP-9 genes. It indicated that lipophilic components in S. miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes, so as to show the inhibitory effect on angiogenesis.
Angiogenesis Inhibitors
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chemistry
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pharmacology
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Cell Movement
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drug effects
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Cell Proliferation
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drug effects
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Human Umbilical Vein Endothelial Cells
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cytology
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drug effects
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metabolism
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Humans
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Matrix Metalloproteinase 9
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genetics
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metabolism
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Salvia miltiorrhiza
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chemistry
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Vascular Endothelial Growth Factor A
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genetics
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metabolism
6.Association of single nucleotide polymorphism of transcription factor 7-like 2 gene with gestational diabetes mellitus
Yuanchi HUI ; Fan PING ; Wei LI ; Min NIE ; Lihong ZHANG ; Ming LI ; Juntao LIU ; Hongding XIANG
Chinese Journal of Endocrinology and Metabolism 2011;27(1):32-35
Objective To investigate the relationship between gene polymorphism of transcripion factor 7-like 2 (TCF7L2) at positions rs290487, rs11196205, rs11196218 and gestational diabetes mellitus (GDM) in Chinese women.Methods In 1140 unrelated pregnant Northern Chinese women (335 women with GDM, 158 gestational cases with impaired glucose tolerance and 647 pregnant non-diabetic controls) ,three single nucleotide polymorphisms (rs290487, rs11196205, and rs11196218) in the TCF7L2 gene were genotyped using ligase detection reaction (LDR).In the present study, cases with GDM and impaired glucose tolerance (IGT) were indistinguishable clinically and biochemically, and were combined into case group.Results The frequency of C allele of rs290487 was 41.6% in case group, being significantly higher than that in control group (36.3%, P=0.012).There was significant difference in the frequency of CC genotype between case group and control group (18.7% vs 14.0%, P=0.033).Compared with T allele carriers, CC genotype carriers had a 1.418-fold increased risk of GDM (95% CI 1.028-1.955).After adjusting for age, body mass index, family history of diabetes,systolic blood pressure,and diastolic blood pressure, pregnant women with CC genotype carriers of rs290487 were more prone to hyperglycemia compared with the T allele carriers (OR 1.518, 95% CI 1.064-2.166).Conclusions The TCF7L2 rs290487 variant may contribute to the genetic predisposition to GDM.CC genotype is likely to be associated with an increased risk of GDM in the pregnant Chinese women.
7.The intervention-therapeutic effect of lipiodol-arsenic trioxide emulsion for primary hepatic carcinoma
Xiang-Dong LIU ; Wen-Juan WU ; Hai-Tao LI ; Xin-Yun FAN ; Yue-Ming NAN ;
Journal of Interventional Radiology 2006;0(12):-
Objective To discuss the short-term clinical effect including functional change of lipiodol- arsenic trioxide emulsion on the primary hepatic carcinoma.Methods Fifty-two patients undergone arterial chemoemblization were selected and then randomly divided into two groups:treatment group(n=27)and control group(n=25).Patients in treatment group were treated with lipiodol-arsenic trioxide,while those in control group treated with mitomycin,epirubicin,cisplatin or lipiodol.Clinical symptoms and six liver function parameters were observed and analized.Results The clinical symptoms of patients in treatment group improved much better than those in control group,and the liver function impairment of patients in treatment group also decreased more than those in control group.Conclusions Lipiodol-arsenic trioxide is an effective and safe intervention-therapeutic embolization material for primary hepatic carcinoma.
8.Studies on the long-term toxicities of reteplase in macaca fasicular
Liuyi DONG ; Xiang ZUO ; Li FAN ; Ming FANG ; Feihu CHEN ; Qiang ZHI ; Zhiwu CHEN
Chinese Pharmacological Bulletin 1986;0(05):-
AIM To observe the long-term toxicities of reteplase(Ret). METHODS Rat was administrated by injection of vein on 14 consecutive days to macaca fasicular. RESULTS Ret 8.0 MIU?kg -1 to macaca fasicular did not show any toxicity. In 25.0 MIU?kg -1 and 80.0 MIU?kg -1 of doses, RBC, HGB and HCT of blood have significantly decreased. Feces of hidden blood test also showed positive response in some animals, meanwhile, Ret could reduce the content of plasma total protein. But these changes could have recovery in restoration period. In addition, in 8.0?25.0?80.0 MIU?kg -1 doses, Ret could significantly lengthen bleeding time and clotting time. The obvious toxic changes of coefficient and pathology in Ret 8.0?25.0?80.0 MIU?kg -1 groups were not found. CONCLUSION 8.0 MIU?kg -1 is a safety dosage by injection of vein. 25.0,80.0 MIU?kg -1 doses have certain toxic actions, such as anemia and bleeding, but these toxic effects were reversible.
9.Clinicopathologic analysis of epithelioid sarcoma-like hemangioendothelioma.
Hong-xia LI ; Qin-he FAN ; Zhi-hong ZHANG ; Wei-ming ZHANG ; Xiang-shan FAN ; Guo-xin SONG
Chinese Journal of Pathology 2012;41(11):767-768
Antibodies, Monoclonal, Murine-Derived
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metabolism
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Hemangioendothelioma
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metabolism
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pathology
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surgery
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Hemangioendothelioma, Epithelioid
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metabolism
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pathology
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surgery
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Humans
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Lymphatic Metastasis
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Middle Aged
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Platelet Endothelial Cell Adhesion Molecule-1
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metabolism
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Sarcoma
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metabolism
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pathology
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surgery
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Thigh
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Vimentin
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metabolism
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von Willebrand Factor
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metabolism
10.Chemical constituents from culture of Streptomyces sp. CPCC 202950.
Ming-hua CHEN ; Ye-xiang WU ; Biao DONG ; Xiu-yong FAN ; Li-yan YU ; Wei JIANG ; Shu-yi SI
China Journal of Chinese Materia Medica 2015;40(7):1320-1324
Eleven compounds were isolated from the culture of Streptomyces sp. CPCC 202950 by a combination of various chromatographic techniques including column chromatography over macroporous resin HP-20, MCI, and reversed-phase HPLC. Their structures were identified as 1H-pyrrole-2-carboxamide(1),5'-deoxy-5'-methylthioinosine(2), vanillamide(3), trans-3-methylthioacrylamide(4), 1,2,3,4-Tetraydro-1H-pyrido[3,4-b]indole-3-carboxylic acid(5), cyclo(L-pro-L-tyr) (6), N-[2-(4-hydroxyphenyl)]ethylacetamide(7), benzamide (8), cyclo ('L-leucyl-trans-4-hydroxy-L-proline)(9), cyclo-(Phe-Gly) (10), and tryptophan (11). Among them, compounds 1 and 2 were new natural products. In the preliminary assays, none of the compounds exhibited obvious inhibition of HIV-1 protease activity (IC50 > 10 micromol x L(-1)).
Culture Media
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chemistry
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metabolism
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HIV Protease
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analysis
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HIV Protease Inhibitors
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chemistry
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isolation & purification
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Molecular Structure
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Spectrometry, Mass, Electrospray Ionization
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Streptomyces
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chemistry
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metabolism