1.Experimental Study on Liq Qibei against Herpes Simplex Virus-Ⅱ in Vitro
Hongyi DENG ; Huizhong JIANG ; Xiang YU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(10):-
Objective To explore the effect and mechanism of Liq Qibei against herpes simplex virus-Ⅱ(HSV-Ⅱ) for treatment of cervical erosion.Methods Using micro-inhibition cytopathic and in vitro cell culture technique,the various concentrations of Liq Qibei in inhibiting HSV-Ⅱ proliferation and the direct virucidal effect were studied.The virus titer and virus inhibition index were taken as parameters.Results ① Virus proliferation experiment:Compared with control group,the virus titer of 1.63,0.81,0.41 mg/mL Liq Qibei group had significant difference(P2.② Direct virucidal effect of Liq Qibei:comparing with control group,1.63 mg/mL Liq Qibei in 8 h,24 h and 0.81 mg/mL-24 h groups had marked difference(P0.05).Conclusion Liq Qibei antivirus effect on HSV-Ⅱ.
2.Therapeutic Effect of Oral Indomethacin for Patent Ductus Arteriosus in Full-Term Infants
li, DENG ; you-xiang, ZHANG ; li, YU
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To explore therapeutic effect and side effect of oral indomethacin for treating patent ductus arteriosus(PDA)in full-term infants.Methods Forty-one full-term infants confirmed PDA by echocardiographically,who were admitted to the neonatal intensive care unit of our hospital from Jan.2004 to Dec.2007,were randomly divided into experimental group(21 cases)and control group(20 cases).Three oral doses of indomethacin [0.2 mg/(kg?time),at an interval of 12 hours] were administered in experimental group,while nothing in control group.Hepatorenal function and blood routine were measured in both groups in 2 days before and after treatment.Urine output and level of serum blood sugar were measured,and abdominal distension,vomiting,bloating,and bleeding were recorded during treatment.Color Doppler echocardiographic examination was performed,heart murmur was stethoscopied,and the rate of ductal closure was recorded at 5 to 7 days after treatment.The infants were followed up at out-patient department at 6 to 12 months after treatment,color Doppler echocardiographic examination was performed,and ductal closure condition was recorded.Results Hepatorenal function and blood routine were normal in experimental group in 2 days before and after treatment.Except that a little gastrointestinal bleeding occurred in one case of experimental group after the second dose of indomethacin,other adverse reactions were not observed during treatment.The ductus was closed in 16 infants in experimental group,the rate of ductal closure was 76.19%,while the ductus was closed naturally in 5 infants in control group,the rate of ductal closure was 25.0% at 5 to 7 days after treatment.There was significant difference in the rate of ductal closure between the experimental and control groups(?2=10.74 P
3.Study on automatic gain control method of pulse oxygen saturation measured
Wei YU ; Ning ZHANG ; Xiang YAO ; Qinkai DENG
Chinese Medical Equipment Journal 1989;0(02):-
The automatic gain control circuit is designed on the basis of MSP430F149 microcomputer with the chopper amplifier, and then continuous stepless regulation of pulse wave signal is fulfilled. Thus individual variation -induced problems are eliminated such as signal baseline drift and saturation. With this circuit, medical instruments can be portable and low-power-consumption.
4.Study on programmable gain in ultrasonic guidance system for the blind
Xiang YAO ; Yongchun SU ; Wei YU ; Qinkai DENG
Chinese Medical Equipment Journal 1993;0(06):-
Ultrasonic sensors are optimal to the guidance system for the blind.The previous ultrasonic guidance systems acquire the position information of obstacles,and transform it into audio signal or tactile vibration.The system developed in this paper,utilizing programmable gain technique and digital potentiometer,acquires the power of reflected ultrasonic as additional information,and informs the blind with modulated audio signal.In this paper,the studies on programmable gain is described detailed including the hardware structure,the software design and information post processing.
5.Signal processing in monitoring blood oxygen concentration by LMS self-adaptive arithmetic self-adaptive arithmetic
Yongchun SU ; Wei YU ; Xiang YAO ; Feixue LIANG ; Qinkai DENG
Chinese Medical Equipment Journal 2004;0(07):-
Lives are maintained by enough oxygen.Blood oxygen saturation is one of the important parameters which reflects oxygen concentration in blood.The accurate monitoring of blood oxygen saturation is very important to both physiological research and medical application.At present,dual-wavelength method is widely adopted in noninvasive detection of blood oxygen saturation.Therefore,accurate recognition of peak-to-peak value of pulse waveform is a key process in monitoring blood oxygen saturation.In the paper,LMS self-adaptive arithmetic is applied to processing pulse waveform signal.The ratio of corrective detection is improved by the method.
6.Experimental study on non-invasive detection of reflectance oxygen saturation
Wei YU ; Qingen GU ; Xiang YAO ; Feixue LIANG ; Qinkai DENG
Chinese Medical Equipment Journal 2003;0(10):-
Objective To study non-invasive measurement of reflectance oxygen saturation.Methods Diffusion theory was used to explain the action of light in tissue and deduce reflectance oxygen saturation formula.Then,Reflectance Oxygen Saturation Detection System based on MSP430 was developed and calibrated.Result The calibration result and calibration curve was ascertained and analyzed.Conclusion The formula for detection and measurement of oxygen saturation is put forward by using Reflectance Oxygen Saturation Detection System.
7.The effect of hypertonic seawater and isotonic seawater for nasal mucosa of allergic rhinitis mice model.
Zhifeng DENG ; Yu XU ; Jin OU ; Rong XIANG ; Zezhang TAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(23):1880-1883
OBJECTIVE:
To study the effect of hypertonic seawater and isotonic seawater for nasal mucosa of allergic rhinitis mice model, and explore the possible mechanism of nasal irrigation with seawater in treatment of allergic rhinitis.
METHOD:
We used Der pl to make allergic rhinitis model of BALB/c mice, and divided them into three groups randomly. Nasal irrigation with hypertonic seawater (HS) or isotonic seawater (IS) in the treatment group 1-14 days after modeling, and black control (BC) group was given no treatment after modeling. Normal control (NC) group was given no treatment, the number of rubs and sneezings in each group were counted in 30 min after the last nasal irrigation. Mice were then killed 24 h after the last therapy. The noses of mice from each group were removed and fixed, then the slices were stained with hematoxylin and eosin, the others were observed by transmission electron microscope.
RESULT:
Mice with hypertonic seawater and isotonic seawater were significantly improved in rubs and sneezings compared to the black control group (P<0. 05); The number of eosinophiles in mucosal tissues of HS group and IS group had no significant difference with that of the black control group (P> 0. 05); Ciliated columnar epithelium cells in mucosal tissues of HS group and IS group were arranged trimly, better than that in the black control group. Morphology and microstructure in nasal mucosal of HS group was closer to the normal group than in IS group.
CONCLUSION
The injury of nasal mucosa ciliated epithelium was significantly improved by nasal irrigation with hypertonic seawater and isotonic seawater, and the former is better than the latter, the mechanism of nasal irrigation with seawater in treatment of allergic rhinitis may rely on repairing the injured nasal mucosa ciliated epithelium, thereby the symptoms of nasal was reduced.
Animals
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Disease Models, Animal
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Mice
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Mice, Inbred BALB C
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Nasal Lavage
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Nasal Mucosa
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Nose
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Rhinitis, Allergic
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therapy
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Seawater
8.Identification of drug-resistance gene type in Mycobacterium tuberculosis by gene chip in Hunan province
Yehua DENG ; Yangen XIANG ; Xiaohua MA ; Guomin SHI ; Rong YU ; Xuefeng PENG
International Journal of Laboratory Medicine 2015;(22):3223-3226
Objective To investigate the drug resistance of 1 031 Mycobacterium tuberculosis to rifampicin and isoniazide in the Center Hospital of Changsha from January 1 ,2013 to September 30 ,2014 .Methods A total of 1 031 strains with positive culture result and identified as strains of Mycobacterium tuberculosis were used absolute concentration method to do the conventional drug susceptibility ,and detected rifampicin and isoniazide resistance gene including rpoB ,katG and inhA gene locus mutation by chip technology ,the results of two methods were compared using card square test statistics .Results By gene chip method ,the sensitive strain of rifampicin was 896 ,the drug-resistant strains was 135 ,the sensitive strains of isoniazide was 901 strains ,130 drug-resistant strains .Compared with the absolute concentration method ,resistance chip detection results were consistent with rifampicin resistant strains 1 011 strains(including 894 drug-resistant strains ,and 117 sensitive strains) ,the coincidence rate was 98 .00% ,consistent with isoniazideresistant strains 1 005 strains(including 890 drug-resistant strains ,115 sensitive strains) ,the coincidence rate was 97 .48% .The most common spot of rifampin resistance related mutations of rpoB gene was 531TCG to TTG ,accounted for 51 .11% ,followed by 526CAC→TAC ,accounted for 10 .37% ,11 strains with 526TCG to TTG ,accounted for 8 .15% .Isoniazid re-sistance was caused by mutations in katG315AGC→ACC resistant strains ,accounted for 83 .85% ,inhA-15C→T mutations accoun-ted for only 12 .30% .Conclusion The results of gene chip method is highly consistent with that of absolute concentration method , could be a fast and effective method for screening rifampicin and isoniazide ,the resistant gene of Mycobacterium tuberculosis to rif-ampicin and isoniazide almost mutate in rpoB531 ,526 and katG315 in Changsha .
9.Mesenchymal chondrosarcoma of thoracic spine: report of a case.
Xiang-lei HE ; Deng PAN ; Yu ZHOU ; Yong-li GAN ; Hong-bin ZHANG
Chinese Journal of Pathology 2006;35(12):766-767
12E7 Antigen
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Antigens, CD
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metabolism
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Cell Adhesion Molecules
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metabolism
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Child, Preschool
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Chondrosarcoma, Mesenchymal
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Humans
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Immunohistochemistry
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Male
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Neuroblastoma
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metabolism
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pathology
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Sarcoma, Ewing
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metabolism
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pathology
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Spinal Cord Neoplasms
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metabolism
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pathology
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surgery
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Spinal Neoplasms
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metabolism
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pathology
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Thoracic Vertebrae
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Vimentin
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metabolism
10.Antisense phosphorothioate oligonucleotide targeting bFGF enhances chemosensitivity of human laryngeal squamous carcinoma cells
Hong-Liang HUANG ; Hong WANG ; Jun-Jian XIANG ; Yong TANG ; Ning DENG ; Hong-Yu YANG ;
Chinese Journal of Cancer Biotherapy 2006;0(05):-
Objective:To study the enhancing effect of bFGF-targeted antisense phosphorothioate oligonucleotide (APO)on the chemosensitivity of human laryngeal squamous carcinoma cell line Hep2 to Doxorubicin,5-Fluorouracil, and Cisplatin.Methods:bFGF-specific APO was designed,constructed and transfected into Hep2 cells with jetPEI (polyethyleneimine).Expression of bFGF mRNA was evaluated by semi-quantitative RT-PCR after transfection;immuno- cytochemical method was used to examine the expression of bEGF expression before and after transfection of Hep2;the in- duction of cell apoptosis was analyzed by flow cytometry;cell proliferation was then analYzed by MTT assay after treatment with bFGF-specific APO or chemotherapeutic drugs,or a combination of both.Results:bFGF-specific APO inhibited the growth of Hep2 cells in a dose-and time-dependent manner,with the peak inhibitory rate being 25.5%.The expression of bFGF mRNA and protein decreased by 52.0% and 41.1%,respectively.The apoptosis rate of Hep2 cells was 20.5% after transfection,bFGF-specifie APO reduced the 50% inhibitory concentration of Doxorubicin,5-Fluorouracil,and Cis- platin in Hep2 cells by 75.5%,83.5% and 65.4%,respectively.Conclusion:bFGF-specific APO can enhance the chemosensitivity of Hep2 cells,which paves a new way for potential biologic chemotherapy of laryngeal squamous carcino- ma.