1.Clinical analysis of chronic barium poisoning.
Sheng-Jun MENG ; Qing-Long LU ; Zeng-Xiang MAS
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(6):370-371
Adolescent
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Adult
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Barium
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poisoning
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Chronic Disease
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Female
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Humans
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Male
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Middle Aged
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Occupational Diseases
;
diagnosis
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therapy
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Retrospective Studies
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Young Adult
2.32 patients of acute fulminant paraquat poisoning.
Wen-Sheng WANG ; Qing-Long LU ; Zeng-Xiang MA
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(12):772-773
Acute Disease
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Adolescent
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Adult
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Female
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Humans
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Male
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Middle Aged
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Paraquat
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poisoning
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Retrospective Studies
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Young Adult
3.The automatic optimization arithmetic of selecting beam arcs in X-knife radiotherapy planning system.
Yin-xiang LIU ; Shu-xiang LI ; Qing-wen LU
Chinese Journal of Medical Instrumentation 2002;26(1):20-22
In this paper, two new concepts of DR (Dose Repulsion) and DG (Dose Gravitation) are presented with their calculation formulas. For the problem of selecting beam arcs in x-knife radiotherapy Planning system, a mathematics model of constrained optimization has been built. Furthermore, we have produced a feasible project of automatic selecting optimized beam arcs plan using SA (Simulated Annealing) arithmetic based on the distribution of the fields of DR and DG in the reduced phase space.
Algorithms
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Brain Neoplasms
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radiotherapy
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Models, Theoretical
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Radiotherapy Dosage
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Radiotherapy Planning, Computer-Assisted
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methods
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Radiotherapy, Conformal
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methods
5.Clinical significance of measurement of glycated albumin in impaired glucose regulation and newly diagnosed diabetes
Junling TANG ; Xiaojing MA ; Huijuan LU ; Jian ZHOU ; Qing LI ; Weiping JIA ; Kunsan XIANG
Chinese Journal of Endocrinology and Metabolism 2008;24(6):630-632
Objective To evaluate the clinical significance of glycated albumin (GA), a parameter in reflecting recent glycemic control, in patients with impaired glucose regulation (IGR) and newly diagnosed diabetes mellitus. Methods From January to June 2007, 516 subjects who accepted oral glucose tolerance test (OGTT) in out-patient department were randomly enrolled, including 130 with normal glucose regulation (NGR), 154 with IGR and 232 with diabetes mellitus. The fasting plasma glucose (FPG), 0.5 h plasma glucose (PG), 1hPG, 2hPG, 3hPG after glucose loading, HbA1c and GA were measured in each subject. The correlation between GA and the other parameters were analyzed. Results There were significant differences in the values of GA among NGR, IGR and diabetes mellitus groups (all P < 0.01), but no difference in the values of HbA1c between NGR group and IGR group (P > 0.05). GA was positively correlated with HbA1c (r = 0.75, P < 0.01). Stepwise regression analysis showed that FPG, 2hPG and body mass index were the major independent contributing factors. Conclusion The combination of plasma glucose with GA measurement may be helpful in evaluating the status of glucose metabolism.
6.p73 gene expression in apoptotic process of acute myeloid leukemia cell line U937 induced by methotrexate.
Guang-Fen XIAO ; Qing LU ; Xiang-Dong YANG
Journal of Experimental Hematology 2002;10(2):104-107
The purpose of this investigation was to study the variation of p73 gene expression in the apoptotic process of acute myeloid leukemia (AML) cell line U937 induced by methotrexate (MTX). Morphological changes of apoptotic cells were observed with microscopy and Wright's + Giemsa staining. DNA ladder and cell cycle were examined by agarose gel electrophoresis and flow cytometry respectively. Using semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR), the expression of p73 mRNA was examined. Results showed that MTX could induce U937 cell apoptosis effectively. Condensed nuclei, fragmentation of chromosome and DNA ladder were seen after 6 hour following treatment of MTX 5 micro mol/L. Sub-G(1) peak and S + G(2)/M arrest were also determined by FCM, but the quantity of p73 expression was generally constant. In conclusion, U937 cell apoptosis induced by MTX did not change p73 mRNA level.
Acute Disease
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Antimetabolites, Antineoplastic
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pharmacology
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Apoptosis
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drug effects
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genetics
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Cell Cycle
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drug effects
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Cell Division
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drug effects
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DNA, Neoplasm
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drug effects
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genetics
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metabolism
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DNA-Binding Proteins
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genetics
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Flow Cytometry
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Gene Expression Regulation, Neoplastic
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drug effects
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Genes, Tumor Suppressor
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Humans
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Leukemia, Myeloid
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drug therapy
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genetics
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pathology
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Methotrexate
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pharmacology
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Nuclear Proteins
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genetics
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RNA, Messenger
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drug effects
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genetics
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metabolism
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Tumor Protein p73
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Tumor Suppressor Proteins
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U937 Cells
7.Application of wave intensity technique on assessing hemodynamic changes in hyperthyroidism
Meiling WANG ; Yanrong ZHANG ; Qing Lü ; Mingxing XIE ; Yehua YIN ; Chengfa LU ; Tianwei YAN ; Yan HUANG ; Feixiang XIANG
Chinese Journal of Ultrasonography 2009;18(8):675-679
lusions WI parameters reflect both cardiac and vascular changes of hyperthyroid patients. Mid-systolic hemodynamic turbulence appears in a portion of hyperthyroid patients.
8.The diagnostic value of echocardiography in cardiac amloidosis
Li ZHANG ; Qing Lü ; Mingxing XIE ; Xinfang WANG ; Yali YANG ; Lin HE ; Xiaofang LU ; Jing WANG ; Feixiang XIANG ; Ming CHEN
Chinese Journal of Ultrasonography 2009;18(1):11-13
Objective To analyze transthoracic echocardiographic characteristics of cardiac amloidosis (CA).Methods Fifteen cases of biopsy-proven CA were retrospectively reviewed.Results Interventricular septal thickening(>1.3 cm)of varing degrees were found in all cases.In 11 cases,the posterior of left ventricular wall were thickened.Myocardial echoes of interventricular septum and free wall of left ventricular were enhanced evidently and distributed unevenly.The echoes presented as ground glass-like images,with some spotty hyper echoes.Both atria were enlarged,and LVEF decreased,with diastolic function impaired,and mild-moderate hydropericardium found in the CA.Conclusions Transthoracic echocardiography is a relatively sensitive and highly specific non-invasive mothod tO diagnose CA.
9.Significance of miR-155, miR-34a and miR-30a expression in diffuse large B-cell lymphoma.
Guan-xing SUN ; Xiang-shan CAO ; Qing LI ; Zhilin WANG ; Jing PENG ; Chang-qing LU
Chinese Journal of Medical Genetics 2013;30(1):79-83
OBJECTIVETo determine the expressions of miR-155, miR-34a and miR-30a in diffuse large B-cell lymphoma (DLBCL) and to explore their potential correlation with clinicopathological characteristics.
METHODSThe expression level of miR-155, miR-34a and miR-30a in 46 DLBCL samples were determined with TaqMan real-time polymerase chain reaction. Interphase fluorescence in situ hybridization (I-FISH) was performed to detect MYC and p53 genes' status, and immunohistochemistry (Envision method) was used to evaluate the expression of CD3, CD10, CD20, BCL-6 and MUM-1 in DLBCL. The DLBCLs were classified into germinal center B cell-like (GCB) and non germinal center B cell-like (non-GCB) subtypes according to Hans' criteria.
RESULTSCompared with normal controls, miR-155 expression level was significantly higher in DLBCL. The expression level of miR-155 in non-GCB type was higher than that in GCB type. It was shown that the patients with MYC rearrangement had lower expression level of miR-155 than the negative controls. Compared with p53 normal group, the expression level of miR-34a was significantly lower in p53 deletion group. It was also shown that the patients with BCL-6 protein expression had lower expression of miR-30a compared with the negative group.
CONCLUSIONmiR-155 expression level is different in normal controls, DLBCL and patients with subtype DLBCL. It therefore has a diagnosis value for DLBCL. miR-34a is of great prognostic significance. miR-155, miR-34a and miR-30a may be potential therapy targets for DLBCL.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Lymphoma, Large B-Cell, Diffuse ; genetics ; metabolism ; pathology ; Male ; MicroRNAs ; genetics ; metabolism ; Middle Aged ; Proto-Oncogene Proteins c-myc ; genetics ; metabolism ; Tumor Suppressor Protein p53 ; genetics ; metabolism ; Young Adult
10.Limb preservation surgery combined with perioperative rehabilitation for the treatment of 7 patients with stage II to III giant cell tumor of bone in the proximal humerus.
Wan-Qing LU ; Qing-Tian XIANG ; Hong-Guang ZUO ; Ya-Jun YU ; Zhen-Guo PAN ; Feng-Dong ZHAO
China Journal of Orthopaedics and Traumatology 2011;24(12):1032-1035
OBJECTIVETo study the effects of the extensive resection of the tumor-loading segment and artificial humerus head replacement combined with perioperative rehabilitation for the treatment of stage II to III giant cell tumor of bone in the proximal humerus.
METHODSFrom March 2007 to March 2010, 7 patients with stage II to III giant cell tumor of bone in the proximal humerus were treated. Among the patients, 3 patients were male and 4 patients were female with a mean age of 34.6 years (ranged, 18 to 49 years). The mean course of disease was 19 months (ranged, 6 to 35 months). All the patients were confirmed to suffer stage II to III giant cell tumor of bone in the proximal humerus by pathology and X-ray examinations. Clinical manifestations of the patients included persistence aggravated pain of the shoulder, swelling in the proximate arm with obviously tenderness, activity limited of the joint. All the patients were treated with extensive resection of the tumor-loading segment and artificial humerus head replacement combined with perioperative rehabilitation. CMS and OSIS score system were used to evaluate shoulder function and shoulder stability.
RESULTSAll the patients were followed up, and the duration ranged from 14 to 35 months, with an average of 17 months. There were no serious complications or recurrence in all cases. One year after the surgery CMS and OSIS score system were 70.7 scores (ranged,63 to 82 scores) and 25.1 scores (ranged, 18 to 29 scores) respectively. According to evaluation for shoulder function, 2 patients got an excellent result and 5 good. According to evaluation of shoulder stability, 1 patient got an excellent result and 6 good.
CONCLUSIONExtensive resection of the tumor-loading segment and artificial humerus head replacement combined with perioperative rehabilitation for the treatment of stage II to III giant cell tumor of bone in the proximal humerus would not only preserve the upper extremity but also preserve the function of upper extremity.
Adolescent ; Adult ; Bone Neoplasms ; pathology ; rehabilitation ; surgery ; Female ; Giant Cell Tumor of Bone ; pathology ; rehabilitation ; surgery ; Humans ; Humerus ; surgery ; Limb Salvage ; Male ; Middle Aged ; Neoplasm Staging