AIM: To investigate whether berberine can ind uce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to di fferentiate by berberine. The morphological changes of MSCs were evaluated by li g ht microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by be rberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differe ntiate into neuron-like cells in vitro.

Objective To explore the expression of forkhead box protein 3 (FOXP3) in gastric cancer cells,and its relationship with clinicpathological characteristics and prognosis of gastric cancer patients.Methods The expression profile of FOXP3 in 111 gastric adenocarcinoma tissues and 20 normal gastric mucosa were detected with immunohistochemistry,statistical analysis combined with clinical data of patients with gastric cancer.Results Positive staining of FOXP3 was mainly located in the nucleus of gastric adenocarcinoma cells with the positive rate of 42.3％ (47/112).In the normal gastric tissue,the positive rate of FOXP3 was 0％ (0/20).There were significant difference (x2 =13.207,P ＜0.05) when comparison was made between gastric adenocarcinoma and normal gastric tissue groups.Comparison of FOXP3 positive rate between gastric adenocarcinoma patients and its histological subtype (tumor size,depth of invasion,and clinical stage) showed significant difference (P ＜0.05),but (sex,age,and staging of tumor node metastasis (TNM),lympho node metastasis and differentiation) showed no significant difference (P ＞ 0.05).In the survival analysis,there was no significance in survival rate between the FOXP3 positive group and FOXP3 negative group either (P ＞ 0.05).Conclusions The FOXP3 were expressed in the human gastric cancer specimens.To a certain extent,FOXP3 was involved in the development of gastric adenocarcinoma and related to the degree of malignancy,but is not related to the prognosis of gastric cancer.

Objective To explore the variance of helicobacter pylori(Hp) infection rate in children with recurrent abdominal pain(RAP).Methods By using the()~(13)C-urea breath test(~(13)C-UBT) to detect the status of Hp infection in 1676 children with RAP from 1998 to 2004.Results 1.Total 1676 cases of children with RAP were detected.There were 438 cases showed Hp positive,the positive rate was 26.13%.The infection rates in patients from 2-5,5-10,and 10-14 years of age were 23.66%(84/355),(26.31%)(286/1087),29.06%(68/234),respectively.2.Hp infection rates in children with RAP from 1998 to 2004 were 29.33%(83/283),(28.90%)(76/263),25.76%(51/198),26.01%(56/215),25.81%(48/186),24.45%(67/274),22.18%(57/257),respectively.Conclusion 1.The Hp infection rate in children with RAP increased with age;2.The Hp infection rate in children with RAP decreased year after year.

Objective To study the rationality in the use of proton pump inhibitors (PPI) to stress ulcer(SU) prophylaxis in pa-tients receiving aseptic-contaminative and contaminative incision operation .Methods A retrospective analysis was performed on the use of PPI to SU ,which taken from surgical patients received aseptic-contaminative and contaminative incision operation and hospi-talized from January 2013 to April 2013 .Results Among 1 745 patients in the study ,540(30 .95% ) received a stress ulcer prophy-laxis with PPI .The rate of prophylaxis to ICU ,general surgery ,urology ,thoracic surgery and neurosurgery was higher than other departments(except for between ICU ,thoracic surgery ,neurosurgery and orthopedic) .A total of 232 patients had risk factors , 72 .41% of the patients who received prophylaxis .A total of 1 513 patients did not have risk factors ,24 .59% of the patients who received prophylaxis ,the rate of prophylaxis to general surgery ,thoracic surgery and urology was higher than other departments (except for ICU ,and between thoracic surgery with orthopedics ) .In prevention of SU ,the usage rate of Lansoprazole was higher than that of Omeprazole and Pantoprazole .Preoperative prophylactic usage rate was 14 .44% .Excessive dosage was 0 .93% .Preven-tive medicine treatment longer accounted for 12 .78% .Conclusion The phenomenon such as non-risk factors ,inappropriate of medi-cation time and long course of treatment are existed ,when physicians use the PPI for SU prophylaxis in surgical patients receiving aseptic-contaminative and contaminative incision operation .Therefore ,treatment recommendations for SU prophylaxis are needed to restrict PPI use for justified indications .

Institute for International Medical Education(IIME) develops the concept of global minimum essential requirements,in which the knowledge and skills of"group health"are ranked as the important parts of training the qualified medical students. But in China,the medi-cal colleges almost run the schools according to the traditional teaching system. Whether teach-ing the knowledge of group health is consistent with the current personnel training requirements deserves our attention. Based on the investigation of teaching status about the medical students "group health"knowledge,the author finds the problems in the field of education with regard to the knowledge and skills and puts forward specific measures to improve it.

In order to build a high-quality teaching group with excellent academics,strong innovation and comprehensive ability, the School of Public Health of CQUMS pays much attention to training young teachers,encouraging them to further their study and scientific research abilities as well as improving their comprehensive abilities.

According to the teaching situation,associated with common problems which influence paper quality,we discussed how to control students' paper quality on preventive medicine,such as adopting strict defense procedure,making score standard of defense,and so on.

Based on their multiple years of personal experiences in hospital administration,authors of this article point out that medical service,humanistic service,and emotional service should be the core aspects in the current hospital competition.Medical professionalism embodied in the three levels of consciousness,behaviors,and regulations should be the main subject in the research on the role of medical ethics in hospital administration and medical treatment behaviors.It is an urgent ethical requirement and the chief task for hospital administrators to achieve humanistic medical service through humanistic administration of hospitals.

China ; Clinical Trials as Topic ; methods ; Humans ; Organizational Policy ; Publishing ; standards ; Registries

Objective To construct and express a recombinant plasmid pGEX-Sj26GST of Schistosoma japonicum(Sj) in Escherichia coli(E.coli) BL21 (DE3).Methods Total RNA was extracted from Sj adult worms by RNeasy Mini kit,26 kilodalton glutathione-S-transferases of Schistosomajaponicum (Sj26GST) antigen gene was amplified by real-time PCR(RT-PCR) from the total RNA,then cloned into a prokaryotic expression plasmid pGEX1λT and transformed into E.coli BL21 (DE3) to construct pGEX-Sj26GST; BL21 (pGEX-Sj26GST) was induced with isopropyl-beta-D-thiogalactopyranoside (IPTG),and the expressed products were analyzed and identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and Western blotting.Results The 676 bp Sj26GST gene was successfully amplified by RT-PCR and restriction enzyme double-digestion technique confirmed that Sj26GST antigen gene was successfully cloned into pGEX-1λT vector,the relative molecular mass of the expressed recombinant protein was approximately 52 × 103 by SDS-PAGE,and the amount of expressed protein was 20％ of the total bacterial proteins; the fusion protein could be recognized by sera from rabbits infected with Sj by Western blotting.Conclusions The recombinant plasmid pGEX-Sj26GST is successfully constructed and highly expressed in E.coli and the expressed fusion protein shows specific antigenicity.