1.Expression of WWOX protein and its significance in non-small cell lung cancers.
Yu-long ZHOU ; Yong-jian XU ; Zhen-xiang ZHANG
Chinese Journal of Oncology 2007;29(4):297-297
Adenocarcinoma
;
metabolism
;
pathology
;
Adult
;
Aged
;
Carcinoma, Non-Small-Cell Lung
;
metabolism
;
pathology
;
Carcinoma, Squamous Cell
;
metabolism
;
pathology
;
Female
;
Humans
;
Immunohistochemistry
;
Lung
;
chemistry
;
pathology
;
Lung Neoplasms
;
metabolism
;
pathology
;
Male
;
Middle Aged
;
Oxidoreductases
;
metabolism
;
Sex Factors
;
Smoking
;
Tumor Suppressor Proteins
;
metabolism
;
WW Domain-Containing Oxidoreductase
4.The value of isotropic scanning and lung care software of 16-slice spiral CT in the diagnosis of solitary pulmonary nodules
Rui XU ; Jian-Long LI ; Cheng XIANG ; Yu-Qing SHAN ;
Cancer Research and Clinic 2006;0(12):-
Objective To evaluate the diagnostic evaluation of isotropic scanning and Lung Care soft- ware in solitary pulmonary nodules,and to improve the diagnostic accuracy.Methods 52 patients suffered from SPN were included in our study.Two experts in CT analyzed the films.First,they read the axial images and made diagnosis.Then isotropic scanning and lung care software approaches were used on 16 spiral CT and another analysis were made again.The results were compared with pathological diagnosis respectively. Results Spiculated sign,lobulated sign,vessel convergence were found more on isotropic scanning approach, that had significant difference with axial images analysis(P
5.Serodiagnosis of trichinellosis by ELISA using recombinant nudix hydrolase of Trichinella spiralis
Shao Rong Long, Xiang Yu Tian, Zhong Quan Wang
Tropical Biomedicine 2015;32(4):669-675
Trichinella spiralis nudix hydrolase (TsNd) gene encoding a 46 kDa protein was
expressed in Escherichia coli and the potential of recombinant TsNd protein (rTsNd) as an
antigen for the serodiagnosis of trichinellosis was investigated by ELISA and compared with
those of ELISA with T. spiralis muscle larval excretory–secretory (ES) antigens. The sensitivity
of both ELISA was 100% (30/30), for the detection of anti-Trichinella IgG antibodies in sera of
the experimentally infected mice, and the specificity of rTsNd-ELISA and ES-ELISA was 100%
(54/54) and 98% (53/54), respectively (P>0.05). Serum anti-Trichinella antibodies were firstly
detected by rTsNd-ELISA at 14 days post infection (dpi), then continued to increase with a
detection rate of 100% at 36 dpi. The anti-Trichinella antibody levels at different times after
infection were statistically different (P<0.05). The results showed that the rTsNd might be a
potential candidate antigen for specific serodiagnosis of trichinellosis. But, it needs to be
further evaluated with sera of the patients with trichinellosis and other helminthiasis.
6.In vitro study of different Chinese herbs on the proliferation and COMP expression of chondrocyte
Yue-Long CAO ; Wei FENG ; Xiang WANG ; Yu XU ; Hong-Sheng ZHAN ; Yin-Yu SHI ;
Chinese Journal of Rheumatology 2003;0(08):-
Objective To investigate the effect of different Chinese herbs on cell proliferation and cartilage oligomeric matrix protein(COMP)expression in chondrocyte culture.Methods Chondrocytes isolated from rabbit knee cartilage were cultured for 3 generations with the density of 2?10~4/cm~2 and were verified by collagenⅡimmunohistochemical staining.Rabbit sera containing herbs were obtained after animals orally ad- ministrated herbs at the dosage equivalent to human.At 5% and 10% serum density,cells were cultured in the medium that contained liver-softening herbal compound sera.Subgroups setting at 1,3 and 5 hours after herb intervention were observed.Rabbit and bovine sera were control groups.Seven days after intervention,chon- drocytes proliferation was observed using the MTT assay kit.For the study of COMP expression,chondrocytes were isolated from human knee cartilage supematant.Superuatant COMP level was tested by enzyme-linked immunoabsorbent assays(ELISA)after directly adding compound and extract from liver-softening herbs to the culture at the final concentration of 10 mg/ml for 3 days.Results Liver-softening herbal compound group had significant effect on cell proliferation compared to control,of which,3-hour subgroup was more significant than 1-and 5-hour subgroups(P
7.Effects of inhaled nitric oxide on hemodynamics and oxygenation in piglets with sepsis
Ding LONG ; Li YU ; Xiaoling WU ; Bo HU ; Hui XIANG ; Tao XU
The Journal of Practical Medicine 2014;(20):3225-3228
Objective To explore the changes in cardiac index and oxygenation index in sepsis piglets after nitric oxide (NO) gas inhalation. Methods A piglet model of sepsis was induced by intravenous infusion of Gram-negative bacterial endotoxin (LPS), then the piglets were randomly divided into two groups. NO group (n=8) was administered with inhaled nitric oxide of 80ppm via volume control (VC) mechanical ventilation for one hour, while the control group (n = 4) received mechanical ventilation with VC and was observed for one hour to assess the stability of the model. The parameters of oxygenation and hemodynamics were measured by PICCO and arterial blood gas analysis every fifteen minutes for one hour. Results Injection of endotoxin induced a stable pig model of sepsis. PH, HCO3-, arterial oxygen pressure (PaO2), mean arterial pressure (MAP) and cardiac index in this model were significantly lower the baseline values (P < 0.01). Arterial oxygen pressure and cardiac index were significantly higher in N0 group than in the control group (P<0.01). Heart rate (HR), central venous pressure (CVP), global end-diastolic volume index (GEDI) and intrathoracic blood volume index (ITBI) did not significantly differ between NO group and the control group. Conclusions Inhalation of nitric oxide gas can significantly improve oxygenation and cardiac function in piglets with sepsis.
8.Cervical carcinoid with high-grade intraepithelial neoplasia: report of a case.
Hai LI ; Fang BAO ; Yu-fei LI ; Yi-long DAI ; Ying XIANG ; Zhi-hong ZHANG
Chinese Journal of Pathology 2013;42(5):347-348
Adult
;
Breast Neoplasms
;
metabolism
;
pathology
;
secondary
;
Carcinoid Tumor
;
metabolism
;
pathology
;
surgery
;
Carcinoma, Adenoid Cystic
;
pathology
;
Carcinoma, Lobular
;
metabolism
;
pathology
;
secondary
;
Cervical Intraepithelial Neoplasia
;
metabolism
;
pathology
;
surgery
;
Chromogranin A
;
metabolism
;
Diagnosis, Differential
;
Female
;
Humans
;
Hysterectomy
;
Keratins
;
metabolism
;
Neoplasms, Multiple Primary
;
metabolism
;
pathology
;
surgery
;
Ovarian Neoplasms
;
metabolism
;
pathology
;
Sex Cord-Gonadal Stromal Tumors
;
metabolism
;
pathology
;
Synaptophysin
;
metabolism
;
Uterine Cervical Neoplasms
;
metabolism
;
pathology
;
surgery
9.Impact of enteral nutrition or parenteral nutrition in post-operative colorectal cancer patients on viscera organ functions and "passing wind" time
Huizhi YU ; Xiang LONG ; Chongmei LIU ; Yanli CAO ; Sixin LI ; Xiaoqiu WU
Chinese Journal of Clinical Nutrition 2009;17(5):268-270
Objective To study the impact of enteral nutrition (EN) or parenteral nutrition (PN) in postoperative colorectal cancer patients on viscera organ function and "passing wind" time.Methods Totally 30 patients with colorectal cancer joined this study with informed consent.Patients were randomly divided into EN group and PN group.Both two groups were given nutritional support from the first post-operative day to the 7th post-operative day.The pre-operative and post-operative viscera organ functions and the recovery time of gastrointestinal functions are observed.Results Total bilirubin was significantly lower in EN group than in PN group (P < 0.05).The length of post-operative hospital stay was significantly shorter in EN group than in PN group (P <0.01).The post-operative complications were not significantly different between two groups (P > 0.05).Conclusion Postoperative EN support is beneficial for colorectal cancer patient in terms of lower bilirubin levels,shorter post-operative hospital stay,and lower cost.
10.Study on effect and correlation of thyroid hormone level changes in peritoneal dialysis patients by thyroxin tablet
Shaxi OUYANG ; Ying CHEN ; An-lan HUANG ; Yu-mei LIANG ; Shu-yi QIAN ; Xiang-dang LONG ;
Chinese Journal of Biochemical Pharmaceutics 2015;(3):92-94,97
Objective To investigate the changes of thyroid hormone in peritoneal dialysis patients and analyse its impact factors, as well as the therapeutic effects of small dose of thyroxine.Methods 150 uremic patients in Hunan Provincial People’s Hospital from December 2013 to December 2014 were selected, 70 cases of uremia non-dialysis patients were divided into group A, while 80 uremia peritoneal dialysis for more than half a year were divided into group B.70 cases healthy examinees during the same period in our hospital were selected as control group ( group C ) . The total triiodothyronine (T3), total thyroxine (T4), free triiodothyronine (FT3), free thyroxine (FT4), thyroid stimulating hormone (TSH), hemoglobin (Hb), serum albumin (ALB), total cholesterol (TC), triglyceride (TG), serum creatinine (SCr), C reactive protein (CRP) and left ventricular ejection fraction ( LVEF) , subjective global assessment of nutritional act ( SGA) and other indicators were detected in three groups.Patients in group B were divided into two sub-groups according to thyroid hormone levels: B1 group had normal thyroid level while B2 abnormal.And the administration of small dose of thyroid hormone was given to patients in group B2, and the effect of the administration was evaluated by the above indexes.Results The FT3 in group A and B were significantly lower than that in group C (P<0.01).There were significant differences of levels of ALB, CRP, SGA between group B1 and group B2, and the FT3 level in group B was significant correlated with SGA, ALB, LVEF(r=0.815,P<0.001;r=0.780,P<0.001;r=0.953,P<0.001).After treated with small dose of thyroid hormone, FT3 and LVEF were improved while FT4, TSH, ALB, SGA, CRP were not improved in group B2.Conclusion The thyroid hormone level in patients with continuous ambulatory peritoneal dialysis decreases which is dominated with FT3.The decreased thyroid level is significantly correlated with nutrition ( ALB, SGA) and left ventricular function.The administration of small dose of thyroid hormone can improve the left ventricular systolic function.