1.Retrospective analysis on 1 124 case-times of renal transplantation
Chinese Journal of Organ Transplantation 1996;0(02):-
Objective To sum up the experience of renal transplantation in order to improve transplant effect and enhance the long-term survival and recovery rate.Methods The data of renal transplantation of 1 124 cases who received renal transplantation from Jul. 1978 to Oct. 2001 were summarized. All the possible factors that could influence the transplant effect such as pre-operative preparation, HLA match, donors' quality, surgical procedure, immunosuppressive therapy and so on were analyzed. Results The 1-, 3-, 5-year survival rate (%) of patient/kidney was 83.1/ 70.3, 74.6/ 68.6 and 62.6/ 56.1 respectively. The rate of AR was decreased significantly from 3.09?% only with LXM to 0.89?% with LXM test in combination with HLA serological test. The rate of HAR and AR in 130 cases with additional PRA test were lower than those only with LXM test.Conclusions The well pre-operative preparation, the ideal HLA match, the high quality of donor's kidney and transplant operation were keys for successful renal transplantation. Reduction of the complications, individual and combined medication in reason and long-term follow-up were very important in the long-term survival time.
2.Follow-up of non-functioning renal graft after endovascular treatment with detachable coils
Jun XIANG ; Xin LI ; Long LIU ; Lianhui FAN ; Cheng HE
Chinese Journal of Organ Transplantation 2010;31(7):433-435
Objective To discuss the indication, technical points and long-term effects of endovascular embolization for non-functioning renal graft with detachable coils, and to get further evaluation of its practical value.Methods Monitored by DSA, endovascular embolization with detachable coils was performed on 11 patients with non-functioning renal graft.Results Renal arteries all had been successfully blocked in 11 cases.Good recovery without any complication was obtained.Conclusion Endovascular embolization for non-functioning renal graft with detachable coils is safe, minimally invasive and convenient, and can be used as an alternative to the resection of the renal grafts.
3.The value of isotropic scanning and lung care software of 16-slice spiral CT in the diagnosis of solitary pulmonary nodules
Rui XU ; Jian-Long LI ; Cheng XIANG ; Yu-Qing SHAN ;
Cancer Research and Clinic 2006;0(12):-
Objective To evaluate the diagnostic evaluation of isotropic scanning and Lung Care soft- ware in solitary pulmonary nodules,and to improve the diagnostic accuracy.Methods 52 patients suffered from SPN were included in our study.Two experts in CT analyzed the films.First,they read the axial images and made diagnosis.Then isotropic scanning and lung care software approaches were used on 16 spiral CT and another analysis were made again.The results were compared with pathological diagnosis respectively. Results Spiculated sign,lobulated sign,vessel convergence were found more on isotropic scanning approach, that had significant difference with axial images analysis(P
4.Research progress of treatment for amblyopia
Hua, LI ; Qing-Ling, XIANG ; Meng, LIAO ; Long-Qian, LIU
International Eye Science 2011;11(3):385-387
The most common forms of treatment for amblyopia are occlusion therapy and pharmacologic penalization. But these methods can not recover all the visual deficits of amblyopes and there are some disadvantages of the treatment which need to be considered, including adverse effects, compliance and social stigma. Recently, some novel approaches are investigated for improving the effectiveness and compliance of treatment for amblyopia.
5.Application of electric heating pattern instrument in experimental teaching of fixed partial denture technology
Enjun ZUO ; Long JIANG ; Xiaojie LI ; Xiang REN ; Shuhai HU
Chinese Journal of Medical Education Research 2013;(5):510-512
Objective To discuss the effects of applying electric heating pattern instrument in experimental teaching of fixed partial denture technology.Methods Totally 98 prosthodontic undergraduates of 2008 and 2009 grades were selected in this study; 46 students of 2008 grade were taken as control group and 52 students of 2009 grade were taken as experimental group.Students in experimental group made wax patterns with electric heating pattern instruments to melt inlay wax while those in control group made wax patterns with instruments heated by alcohol lamps.Teaching effects were evaluated by experimental test scores and questionnaires.Results Test scores of experimental group were (22.6± 1.8),obviously higher than those of (22.6-± 1.8) in control group (P < 0.05).Satisfaction degree of experimental group were increased significantly compared with that of control group based on the resuits of questionnaire.Conclusions Applying electric heating pattern instrument in making wax patterns in experimental teaching of fixed partial denture technology is easy to operate and can improve the quality of wax pattern and working efficiency as well as enhance students' confidence,therefore it is worthy further spreading.
6.Amiloride slows down calpain-mediated ABCA1 degradation through in-hibition of hypoxia-induced NHE1 expression
Xiangang MO ; Li ZHANG ; Luochao ZHANG ; Long WANG ; Ning XIANG ; Juan YANG ; Xiang SONG
Chinese Journal of Pathophysiology 2015;(11):1992-1997
AIM:To examine the effects of hypoxia on sodium-hydrogen exchange 1 (NHE1) expression, in-tracellular Ca2+concentration ( [ Ca2+] i ) and calpain activity, and to explore the effect of amiloride on adenosine triphos-phate-binding cassette transporter A1 (ABCA1) degradation and its calpain-related mechanism.METHODS:RAW264.7 cells were exposed to hypoxia for 0 h, 12 h, 24 h and 48 h.The cell viability was measured by MTT assay and the expres-sion of NHE1 at mRNA and protein levels was detected by real-time PCR and Western blot.[ Ca2+] i was analyzed by flow cytometry.Calpain activity was assessed by the method of Suc-LLVY-aminoluciferin.Furthermore, the protein levels of ABCA1 in the RAW264.7 cells exposed to hypoxia for 24 h were determined after 6 h or 12 h treatment with NHE1 inhibi-tor amiloride in the presence of cycloheximide.ABCA1 protein levels and calpain activity were detected after 12 h incuba-tion with calpain inhibitor ALLN or intracellular calcium-chelating agent BAPTA.RESULTS: Hypoxia inhibited the cell viability in a time-dependent manner.Hypoxia up-regulated the mRNA and protein expression of NHE1, and increased [ Ca2+] i and calpain activity.Hypoxia increased the degradation of ABCA1 and amiloride slowed down the ABCA1 degra-dation.ALLN or BAPTA increased ABCA1 protein level and decreased calpain activity.CONCLUSION:NHE1 inhibitor amiloride attenuates the calpain-mediated degradation of ABCA1, indicating that hypoxia-induced NHE1 might, at least in part, participate in the ABCA1 degradation.
7.Predictive value of fluid overload for mortality in children with severe sepsis
Jiao CHEN ; Long XIANG ; Zhenjiang BAI ; Jun HUA ; Ying LI ; Xiaozhong LI ; Yanhong LI
Chinese Journal of Applied Clinical Pediatrics 2016;31(10):755-759
Objective To investigate the predictive value of fluid overload for mortality in children with severe sepsis.Methods In this retrospective study,the children with severe sepsis who were admitted to the Pediatric Intensive Care Unit (PICU),Children's Hospital of Soochow University between January 2011 and March 2015.Fluid accumulation was calculated in the first 72 hours after admission.Pediatric index of mortality Ⅱ (PIM2) score was calculated during the first 1 hour after admission.Multivariate Logistic regression analysis assessed the relationship between fluid overload and mortality after adjustment for confounding factors.The predictive value of fluid overload for mortality was assessed by the receiver operating characteristic curve and au area under the receiver-operating-characteristic curve (AUC).Results Of the 199 children admitted,62 cases (31.2%) died during PICU stay.Among the children,133 cases (66.8%) had fluid overload of<5%,55 cases (27.6%)had fluid overload of≥5%-10%,and 11 cases (5.6%) had fluid overload of≥ 10%.Multivariate regression analysis showed that a high fluid overload percent (OR =1.263,95 % CI:1.113-1.434,P < 0.001),a high PIM2 score (OR =1.028,95 % CI:1.012-1.043,P < 0.001) and multiple organ dysfunction syndrome(OR =4.160,95% CI:1.728-10.012,P =0.001) were independent risk factors for mortality in children with severe sepsis.The fluid overload was significantly associated with mortality (OR =1.309,95% CI:1.158-1.480,P <0.001),even after adjustment for age and illness severity assessed by PIM2 scores.Fluid overload achieved AUC of 0.741 (95% CI:0.661-0.820,P < 0.001) for predicting mortality in children with severe sepsis.Conclusion Fluid overload developed during the first 72 hours after admission is independently associated with and predictive of PICU mortality in children with severe sepsis.
8.Cervical carcinoid with high-grade intraepithelial neoplasia: report of a case.
Hai LI ; Fang BAO ; Yu-fei LI ; Yi-long DAI ; Ying XIANG ; Zhi-hong ZHANG
Chinese Journal of Pathology 2013;42(5):347-348
Adult
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Breast Neoplasms
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metabolism
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pathology
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secondary
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Carcinoid Tumor
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metabolism
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pathology
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surgery
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Carcinoma, Adenoid Cystic
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pathology
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Carcinoma, Lobular
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metabolism
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pathology
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secondary
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Cervical Intraepithelial Neoplasia
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metabolism
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pathology
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surgery
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Chromogranin A
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metabolism
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Diagnosis, Differential
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Female
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Humans
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Hysterectomy
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Keratins
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metabolism
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Neoplasms, Multiple Primary
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metabolism
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pathology
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surgery
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Ovarian Neoplasms
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metabolism
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pathology
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Sex Cord-Gonadal Stromal Tumors
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metabolism
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pathology
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Synaptophysin
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metabolism
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Uterine Cervical Neoplasms
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metabolism
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pathology
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surgery
9.Effect of electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) on the expression of EphB2 protein in cortex around cerebral infracted area of rat
Hongliang LI ; Juan XIANG ; Lizhi OUYANG ; Guo CHEN ; Zhihui XUE ; Kangsheng LONG ; Tielang LI
Journal of Acupuncture and Tuina Science 2017;15(1):14-21
Objective:To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusion (MCAO) rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia.
Methods:A total of 180 male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups: postoperative 3 d, postoperative 14 d and postoperative 21 d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride (TTC) staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators.
Results:The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group (P<0.05). The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells (EphB2+) around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group (P<0.05), and the positive cell number was higher than that in the sham operation group (P<0.01). Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2+ and postsynaptic density-95 positive (PSD-95+) were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group (P<0.05).
Conclusion:Electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.