Acetylcholine ; metabolism ; Acetylcholinesterase ; metabolism ; Animals ; Brain ; enzymology ; metabolism ; Female ; Hypoxia, Brain ; physiopathology ; Ischemic Preconditioning ; methods ; Male ; Memory ; physiology ; Mice ; Random Allocation ; Reperfusion Injury ; prevention & control

Objective To analyze the patients with infection before transfusion in onder to avoid misdiagnosis and blood transfusion caused by cross-infection and remind medical staff attention to operation and self-protection.Methods HBsAg,Anti-HCV,Anti-TP,Anti-HIV1/2 were detecced by 2 576 cases of pre-operative,prenatal,blood transfusions before the patient's blood ELISA assay.Results 2 576 cases of infectious markers in patients with serum positive rate was 12.15％.The positive rates of was HBsAg,Anti-HCV,Anti-TP,Anti-HIV1/2:11.06％,2.13％,1.67％,0.00％,HBsAg and Anti-HCV were positive for both the percentage of 0.54％.Conclusion The transfusion of pre-infection detection is necessary for patients with early diagnosis and treatment of infectious diseases to avoid and prevent hospital infections,medical personnel,as well as occupational infection to reduce or avoid blood transfusion caused by medical disputes.

BACKGROUND: Studies have demonstrated that intermittent high glucose can have a more severe impact on vascular endothelial function in comparison with persistent hyperglycemia.OBJECTIVE: To investigate the effect of intermittent high glucose on the proliferation and apoptosis of endothelial progenitor cells (EPCs) from human peripheral blood in vitro as well as the production of malondialdehyde (MDA) and antioxidant. METHODS: Total mononuclear cells were isolated from human peripheral blood by Ficoll density gradient centrifugation and then the cells were placed on fibronectin-coated culture dishes. After 7 days of culture, the adherent cells were identified as EPCs by laser scanning confocal microscope. The cells were synchronized and then stimulated with glucose 5.5 mmol/L (normal control group), 20 mmol/L (constant high glucose group), and 5.5/20 mmol/L (intermittent high glucose group, 5.5 and 20 mmol/L glucose culture solution was changed every 8 hours) for 72 hours. EPCs proliferation and apoptosis was measured by MTT assay and flow cytometry, respectively. The content of MDA and the activity of superoxide dismutase (SOD) in culture solution were detected with colorimetry.RESULTS AND CONCLUSION: After EPCs were exposed to constant high glucose (20 mmol/L) and intermittent high glucose (5.5/20 mmol/L) for 72 hours, proliferated cells were significantly reduced and the apoptosis rate was significantly increased compared with those exposed to normal glucose (P < 0.01). Furthermore, there was a significant increase in MDA contents as well as a significant reduce in SOD activities in the constant high glucose and intermittent high glucose group (P < 0.01), especially in the latter group. These findings indicated that both intermittent high glucose and constant glucose could inhibit the proliferation and promote the apoptosis of EPCs; however, intermittent high glucose appears to worsen the effects on EPCs. This is maybe due to the increased oxidative stress.

Objective:To investigate the polymorphism of HLA-DQA1 genes in Jing nationality of Central Vietnam.Methods:Applied PCR-SSP tecnique to determine the polymorphism of the HLA-DQA1 alleles of 105 healthy children and youth,unrelated individuals in Central of Vietnam.Results:10 HLA-DQA1 alleles were detected of which DQA1*0104 were the most common allele with frequency of 21.3% and lowest frequency is DQA1*0601.Conclusion:The results indicate that HLA-DQA1 alleles polymorphism of Jing nationality in Central Vietnam is different from the other Chinese. [

Objective To investigate the effects of siRNA targeting CD 147 gene on the expression of CD147 in melanoma cell line A375, and on proliferation of these cells. Methods Previously prepared recombinant plasmid pSUPER/CD147 siRNA was used. In this study, the recombinant was transfected into the A375 cells. The mRNA expression of CD147 was measured by semi-quantitive RT-PCR, the proliferation of the cells by MTT assay. Results After the transfection with pSUPER/CD147 siRNA1 and pSU-PER/CD147 siRNA2, the mRNA expression of CD147 in the A375 cells was significantly down-regulated by 90.81% (P

Objective To investigate the relationship between the promoter of IL-12B gene polymorphism and the susceptibility and clinical features of chronic gastritis and duodenal ulcer with or without Helicobacter pylori(Hp) infection in children and adolescent.Methods Mucosal biopsies were obtained from 132 children and adolescent (patient group),including 100 children with chronic gastritis and 32 children with duodenal ulcer,undergoing an upper gastrointestinal endoscopy for dyspeptic symptoms.Biopsy specimens were stained with hematoxilin and eosin (HE),and gastritis was graded according to the Sydney system.Serology,urease test and histology were taken to assess Hp status.Genomic DNA was obtained from peripheral blood or gastric biopsies of patients and 102 healthy children as normal control group.The promoter of IL-12B +1188A/G gene polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing.The genotype distributions and allele frequencies were compared between the study group and the normal control group,and the association of genotypes with clinicopathological features was studied.IL-12B mRNA level expressions in gastric mucosa were confirmed by reverse transcription PCR biopsy-based tests.Results The genotype distributions and allele frequencies of IL-12B +1188A/G gene polymorphisms were similar in gastric upper gastrointestinal diseases and healthy subjects.The IL-12B +1188A/G gene polymorphisms were not associated with Hp status.IL-12B+1188A/G gene polymorphisms did not affect IL-12B mRNA level expressions and were not associated with the degree of antrum chronic inflammation.Conclusions These data suggest that IL-12B+1188A/G gene polymorphisms are not associated with susceptibility to chronic gastritis and duodenal ulcer in children and adolescent.

OBJECTIVE: To investigate the recurrence rate of nasal inverted papilloma treating by endoscopic and non-endoscopic approach. METHOD: A search on Pubmed, Medline, Springer and Elsevier databases was done to collect the reports (2001-2013) concerning different surgery treating nasal inverted papillomas, and meta-analysis was performed with RevMan 5.0 software. RESULT: Twelve papers (2001-2013) concerning the different surgery approach treating nasal inverted papillomas were retrieved. The heterogeneity test indicated that the 12 studies were consistent statistically (Q = 14.64, df = 11, P = 0.20), the data from these 12 studies could be analyzed by fixed effect model. After combination of these data, those of 1012 subjects accepted endoscopic surgical intervention and 359 subjects treating by non-endoscopic surgical intervention were collected. Test of overall effect by fixed effect model showed that the recurrence rate of inverted papilloma was significantly lower in endoscopic group than in non-endoscopic group (OR = 0.49, 95% CI was 0.35-0.69, P < 0.01). Funnel plot implied that publication bias was not obvious. CONCLUSION The recurrence rate of inverted papilloma was significantly lower in endoscopic group than in non-endoscopic group.
Endoscopy ; Humans ; Nasal Surgical Procedures ; methods ; Neoplasm Recurrence, Local ; Nose ; Nose Neoplasms ; surgery ; Papilloma, Inverted ; surgery ; Retrospective Studies

BACKGROUND: Construction of animal models of vertebral artery type of cervical spondylosis and evaluation have become a new issue of concern.OBJECTIVE: To describe the research progress of animal models of vertebral artery type of cervical spondylosis at home and abroad, and to evaluate the advantages and disadvantages of each method. METHODS: The keywords were "cervical vertigo, basal-vertebral ischemia, cervical spondylosis of vertebral artery type, animal, model" in Chinese and English, respectively. CNKI, WanFang, VIP, PubMed, Cochrane Library, and Embase databases were retrieved for the articles published from 1998 to 2018. Chinese and English articles on animal modeling of vertebral artery type of cervical spondylosis were selected according to the inclusion and exclusion diagnosis criteria of vertebral artery type of cervical spondylosis. RESULTS AND CONCLUSION: Through induction and analysis, modeling methods include hardener injection, bone graft compression, dynamic imbalance, blood stasis blocking collaturation, vertebral artery ligation and mixed modeling method. Of them, hardener injection method is most commonly used. Bone graft compression method is the most similar pathogenesis to the human body. However, the disadvantage is that the modeling is difficult and the size of the bone block is various. So the bone is not in the right position. Dynamic imbalance method can cause cervical spine imbalanced by resecting the unilateral muscle and bone. This method has high operability, but has long modeling period. Although ligating or clipping the unilateral vertebral artery is easy to operate, the pathogenesis of vertebral artery type of cervical spondylosis is not just vertebral artery stenosis, which ignores the influence of humoral factors, sympathetic nerves and vertebral nerves on the blood flow velocity and lumen size of vertebral artery. Each method has advantages and disadvantages. The evaluation criteria, time and success rate are different.

Objective To study the correlation between obesity and clustering of major risk factors on cardiovascular disease in the elderly.Methods A cross-sectionai study was conducted.Data from 546 elderlY aged 60-90 were analyzed for the relationship between obesity and clustering of risk factors for cardiovascular diseases,with descriptive statistical methods,x2 test,logistie regression and ROC curve．Results (1)The positive rates of the four major risk factors and clustering appeared an upward trend along with the BMI and or the wc.The overall positive rate of clustering of risk factors among the total 546 elderly people was 59.5％,and 52.5％in males,65.7％in females.(2)As compared with females,males had a higher growth rate of clustering with the elevating of BMI and WC.and odds ratios of the highest-level groups were 13.579 and 2.876 in males and females.Respectively.(3)Both BMI and WC were independent influencing factors for male(P<0.05),and logistic regression coefficients were 0.196 and 0.074 for BMI and WC,respectively.Only WC appeared to be the independent influencing factor for females(P<0.05),while BMI WaS not(P:0.537),with logistic regression coefficient as 0.060 for WC.(4)The combination equation of male's BMI and WC Was CEBMI+WC=0.726 × BMI+0.274 × WC,with the optimum cut-off value as 41.59,and the sensitivity and the specificity were 0.785 and 0.598,respectively．Conclusion Four major risk factors for cardiovascular diseases were often related to obesity while control of BMI and WC were of great significance to the primary prevention of cardiovascular disease．The combined equation of BMI and WC could be used to predict the risk of clustering.

ObjectiveTo observe the effect of megace (MA) on the survival condition of cancer patients in chemotherapy (CT) periods.Methods92 patients with cancer were divided into the MA group (treated with MA+CT) and control group (treated only with CT). The changing of the appetite, normal food amount, weight, gastrointestinal reaction and whole body conditions of two groups were evaluated.ResultsIn the MA group, 52.2% patients had appetite improvement, 47.8% had more food amount, 45.7% gained more weight, 50% had no obvious gastrointestinal reaction such as vomiting and nausea, and 50% had improve the survival condition according to the Karnofsky performance status (KPS) scores (increment >10). In the control group, only 6.5% had appetite improvement, 4.3% had more food amount, 13% gained more weight, 28.3% had no vomiting and nausea, and 15.2% had improved the survival condition. There was a significant difference between two groups (P<0.01).ConclusionMegace is able to reduce nausea and vomiting caused by CT, improve appetite, increase patients weight, protect bone marrow from the inhibition of CT, improve the life quality of cancer patients, and has no evident side-effects.