AIM: To study the analgesia and anti-inflammation effects o f aspirin-niacinamide-zinc complex (WUY). METHODS: In this study , the mice ear swelling, vascular permeability increasing and rats’ paw edema w ere adopted to evaluate the anti-inflammable effects of WUY. And the analgesic effects of WUY were tested by writhing reaction and hot-plate method. R ESULTS: In high and low dose groups of WUY, the degrees of ear swelling were 3.3 and 2.8 mg, the Evans blue induced effusion were 3.1 and 1.2 mg?L -1, and the paw edema volume (1-4 h) were 0.21- 0.13 and 0.23- 0.08 ml, respectively. WUY ( 0.3 and 0.45 mmol?kg -1) prolonged incubation period of hot-plate reaction and showed marked i nhibition effects on writhing induced by acetic acid in mice. CONCLUSION : The analgesic and anti-inflammable effects of WUY are stronger than t hat of ASP.

Objective:To study the effect of Liuwei Dihuang decoction (LW) on secretion of testosterone in the testis leydig cells of senescence accelerated mouse (SAM). Methods:The level of testosterone in the testis leydig cells of SAM with aging and the effect of LW on the secretion of testosterone were observed using cultured testis leydig cells in vitro.Results:The level of testosterone in the testis leydig cells of SAMP8 with aging was significantly decreased and showed a significant difference compared with age-matched SAMR1. Chronic administration of LW (2.5 and 5.0 g/kg) for 5 months significantly ameliorated the secretion of testosterone in SAMP8 compared with control group. Conclusions:The secretory function of testis leydig cells was reduced in SAMP8 with aging. LW ameliorated the secretion of testosterone in the testis leydig cells,indicating that LW could antagonize or delay the deterioration of the testis leydig cells in SAMP8.

Acute Disease ; Angioplasty, Balloon, Coronary ; adverse effects ; Compartment Syndromes ; etiology ; Female ; Humans ; Middle Aged

Objective To summarize the method and experience of microsurgical treatment of intracranial aneurysms in anterior circulation. Methods The clinical data, the skills and experience of 171 cases with aneurysmso of anterior circulation within the latest 2 years. Results One hundred and seventy-one patients under surgery, including 67 PCOA aneurysm, 56 ACoA aneurysm, 32 MCA aneurysm, 10 ICA aneurysm, OphA aneurysm and 3 haimess aneurysm. Accoring to Glasgow Prognostic Scale, there were 3 cases of grade Ⅰ, 6 cases of grade Ⅲ, 14 cases of grade Ⅳ and 148 cases of grade Ⅴ. Conclusion The key points to cure intracranial aneurysms successfully is favourable exposure and the skills of microsurgery; the factors of intluent surgery include forcejudgement in surgery, reasonable choice of aneurysm clips, correctly block in arterious.

Objective To evaluate the clinical application of the tissue Doppler imaging (TDI) in investigating the cardiac longitudinal systolic function in the patients with type 2 diabetes mellitus. Methods Forty-two patients with type 2 diabetes mellitus and 25 volunteers underwent TDI. The long axis myocardial peak systolic velocity (Sm), displacement (Dm) and strain rates (SRs) of 18 segments in different left ventricular walls were measured.Results The Dm and SRs of left ventricular wall in diabetic group dramatically decreased (P<0.01). Meanwhile, the diabetic combined hypertension group had much lower Sm of ventricular middle segments than the control group (P<0.05).Conclusion The stain rate and tissue tracking are sensitive and reliable to evaluate the early local cardiac systolic function in the patients with diabetes mellitus. TDI can be used as an noninvasive method to detect early systolic dysfunction.

AIM: Recent studies showed that stem cells could replace injured cardiomyocyte and increase the number of functional cardiomyocytes. Researching the pertinent literature in China Journal Full-text Database (CJFD) published between 2005 and 2008 indicated that the researches on stem cell transplantation in the treatment of primary dilated cardiomyopathy were few. This study investigated the therapeutic efficacy and security of peripheral blood stem cell transplantation in coronary artery in treatment of dilated cardiomyopathy and its effects on left ventricular function. METHODS: Forty-two patients with dilated cardiomyopathy between December 2006 and September 2007 were enrolled at the Department of Cardiology of First People's Hospital of Yunnan, including twenty-eight males and fourteen females, averagely aged (56?3) years. Inclusive criteria: patients with less than 65 years, left ventricular enlargement, and left ventricular ejection fraction (LVEF) ≤ 45%, and without coronary artery disease after coronary arteriongraphy. Informed consents were obtained from patients. Patients were divided into stem cell transplantation group (n=15) and control group (n=27) on the basis of whether being treated by stem cell transplantation. Patients in the stem cell transplantation group were consecutively administered granulocyte colony-stimulating factor (G-CSF) by hypodermic injection to stimulate bone marrow stem cells themselves based on conventional treatment for five days. Peripheral blood stem cell suspension was disassociated on the 6th day, and the collected suspension was injected into left anterior descending branch over the wire saccule tube for autologous peripheral blood stem cell transplantation. Patients in the control group were administered by conventional treatment of dilated cardiomyopathy. Security and adverse reaction were observed during the mobilization, collection and returning injection of peripheral blood stem cell by coronary artery. Morphous, cardiac function and motion index of left ventricle wall were evaluated using ultrasoundcardiogram before and 3 months after transplantation. Survival rate and incidence rate of heart incidents were compared. RESULTS: Three months after stem cell transplantation in coronary artery, there were a significant decrease in cardiac end-systolic volume (ESV), cardiac end-diastolic volume (EDV) and motion index of left ventricle wall, but a significant increase in LVEF(P

Objective To explore the different effects of patiants with Myocardial infarction(MI)accompany with HF's LSVF and the prognosis between complete reftow and partial reflow.Methods 57 cases with MI accompany with HF were randomly divided into 2 groups:complete reflow group and partial reflow group.The two groups were done low-dose Dobutamine loading experiment seperately before operations.22 cases were treated by complete tallow using PCI,other 35 Patients were dealed with Partial reflow using PCI.To contrast the data of LVSF,WMSI,A/V and LVED between the two groups with UCG before and after PCI.Results The complete reflow group was obviously more arailable than the partial group.Conclusion The complete reflow can improve LVSF of the paticnts with MI accompany with HF.

Adult ; Ear Canal ; Female ; Hearing Loss, Conductive ; surgery ; Humans ; Male ; Mastoid ; surgery ; Middle Aged ; Postoperative Period ; Tympanoplasty

Objective To explore the relationship between quality of life and sub-health state of Guangdong urban dwellers.Methods A total of 2 200 urban dwellers were investigated using SF-36 and Sub-Heahh Measurement Scale Version 1.0(SHMS V1.0).Results As for the scores in PCS,MCS,psychosocial symptom,cognitive function,social adaptability,those of man urban dwellers (respectively (78.89± 15.25),(73.15 ± 15.26),(63.73± 18.82),(62.13± 19.34),(68.04± 17.90)) were higher than those of woman dwellers (respectively(76.98± 14.20),(71.76± 14.72),(60.53± 18.28),(57.00± 17.89),(65.73± 17.07)).The difference was statistically significant (P＜0.05).Bivariate correlation analysis showed that the PCS and MCS of quality of life were positively related with all dimensions of sub-health (P＜0.001).Canonical correlation analysis indicated that vitality,social adaptability,psychosocial symptom and positive emotion were the main factors of sub-health status,and MCS was of quality of life.Conclusion Sub-health state of Guangdong urban dwellers is positively related with quality of life,and the more serious sub-health state is,the worse quality of life is.Therefore,intervention of subhealth and quality of life should be targeted to be strengthened according to the investigation.

AIM: To study the neuroprotective roles of neuroglobin (NGB) over-expression in the SH-SY5Y cells transfected with pAPPswe.METHODS:The plasmid pEGFP-NGB was successfully constructed and transfected into the SH-SY5Y cells, which were pretreated with pAPPswe.MTT assay was applied to detect the effect of NGB over-expres-sion on the cell survival rates.JC-1 staining was used to detect the level of mitochondrial transmembrane potential.The cell apoptosis was analyzed by flow cytometry.The effects of NGB over-expression on the protein level of p-Akt, Akt and caspase-3/9 were determined by Western blotting.The generation of Aβ42 in the cells was measured by ELISA.RE-SULTS:The cell survival rate was remarkably increased after transfection with NGB compared with control group and emp-ty plasmid group (P<0.05).The over-expression of NGB significantly inhibited the decrease in mitochondrial membrane potential induced by pAPPswe.The over-expression of NGB inhibited the apoptosis of the cells.Furthermore, over-expres-sion of NGB not only inhibited the expression of caspase-3 and caspase-9, but also induced the production of p-Akt, which was prevented by LY294002, an inhibitor of PI3K/Akt.The generation of Aβ42 was inhibited in the cells with the over-ex-pression of NGB.CONCLUSION: Over-expression of NGB significantly inhibits the SH-SY5Y cell injuries induced by pAPPswe and inhibits the expression of caspase-3/9, which is tightly related with cell apoptosis.Furthermore, the neuro-protective roles of NGB may be via activating PI3K/Akt signaling pathway.