Objective To investigate the application of Neurological Classification of Spinal Cord Injury (ASIA) in China.MethodsThe articles were retrieved in CNKI website (www.cnki.net),full-text Chinese journal database using the following parameters:searching words:"spinal cord injury" and "ASIA",publication time:1979-2006.Results164 articles were harvested,and 140 relevant papers of them were selected for analysis.ConclusionASIA Classification is widely used in China(Mainland).Constructive suggestions for the modification of this Classification were accumulated,while delayed use and misuse of it were found in some studies.

ObjectiveTo investigate the effect of both conservative and surgical treatment on cervical spinal cord injury without fracture and dislocation.MethodsResultsAll patients had some recovery after both surgical and conservative treatment(P<0.05),but the patients after surgical treatment had a better outcome than those after conservative treatment(P<0.01).ConclusionThe effect of early surgical treatment on cervical spinal cord injury without fracture and dislocation is superior to conservative.

OBJECTIVE To evaluate the roles of p15,p27 gene protein and expression in nasopharyngeal carcinoma(NPC).METHODS The EnVision immunohistochemical method was used to detect the expression of p15,p27 gene protein in nasopharyngeal carcinoma tissues of 43 NPC cases and non-tumor nasopharyngeal tissues of 21 cases.RESULTS ① The positive expression rates of p15,p27 gene protein were 65%,68% in NPC respectively.There were significant differences between NPC and non-tumor group(P0.05).③The positive expression of p15 gene protein was correlated to the positive expression of p27 gene protein(P

ObjectiveTo assess the efficacy and results of cervical double door laminoplasty for the treatment of cervical spinal cord injury without radiographic spinal fracture and dislocation.Methods24 patients (all men) with cervical spinal cord injury without radiographic spinal fracture and dislocation were treated with operation of cervical double door laminoplasty. The follow up period ranged from 3 months to 21 months. ASIA scores of motion and sensation before and after operation were compared.Results After surgical treatment, ASIA scores of motion and sensation of patients were improved from 35.2±7.1 and 72.4±13.1 to 59.3±6.8 and 122.2±15.2 respectively and having a significant difference compared with that before operation (P<0.01).Conclusion Cervical double door laminoplasty, which is characterized by thorough decompression of spinal cord and preserving the posterior supporting tissue as much as possible to maintain the stabilization of cervical spine after operation, is an ideal optional method for cervical spinal cord injury without radiographic spinal fracture and dislocation.

To analyze retrospectively Mr. Xie Xiliang's medical records accumulated in 30 years on direct moxibustion for treating hepatitis B, in which 86 cases were included. Before accepting direct moxibustion, 80.23% of the cases had been treated by conventional Chinese and western medicine, but no satisfactory therapeutic effects were obtained. Direct moxibustion was applied for 7-9 cones at each point selected. Point selection was related to ages. Usually, only a few points were used, and Ganshu (BL 18) and Pishu (BL 20) were used as main points. Shenzhu (GV 12) was added for children, and Zusanli (ST 36) added for adults. The improvement rate in clinical symptoms and physical signs were 100%. In the cases with hepatauxe, splenauxe, liver cirrhosis, and abdominal dropsy diagnozed by the B-ultrasonic examination, the improvement was obvious. Of the total, the rates turning to be negative in HBsAg, HBeAg and HBcAb were 28.85%, 38.46%, and 36.54%, respectively. It took some 10 days to 1 month to begin to show improvement in physical signs and hepetic functions, and about 3-6 months for their restoration. For the five items tested in type B hepatitis, it took 5 months to 1 year, even several years, to show their improvement. It is indicated that direct moxibustion is definitely effective in treating type B hepatitis. It is easy to handle and worth popularizing.
Acupuncture Points ; Adolescent ; Adult ; Age Factors ; Child ; Child, Preschool ; Female ; Hepatitis B ; pathology ; therapy ; Humans ; Male ; Middle Aged ; Moxibustion ; methods ; Retrospective Studies ; Treatment Outcome ; Young Adult

Objective To study the risk factors of hyperthyroid heart diseases(HHD) by analyzing clinical features of patients in order to provide a scientific basis for prevention and treatment of HHD. Methods Nine hundred and eighty two cases were selected as objective from in-patient data of Thyroid Disease Treatment Centre of Shandong Province. The cases were divided into hyperthyroidism group and HHD group. The variables of etiology,sex, age, duration of disease, TSH, FT3, FT4 and TRAb were analyzed by comparative analysis. The risk factors were analyzed by logistic regression. Results The prevalence of hyperthyroidism complicated hyperthyroid heart disease was 7.7%(76/982), age, duration of diseases, FT3, TRAb in the HHD group were [(51.4 ± 11.5), (6.3 ±2.1) years, 21.6 pmol/L, 71.6 U/L], in hyperthyroidism group were [(37.9 ± 9.8), (2.6 ± 1.3) years, 14.9pmol/L, 49.6 U/L]. The differences were statistically significant(u = 9.93,15.23, T = 44954,48792.5, P ＜ 0.05)between the two groups. The factors of the older, higher FT3 and TRAb, longer duration, Graves disease (OR =1.751,1.470,1.483,1.445,1.234) increased the risk of HHD. Conclusions Graves disease, longer duration, old age, higher FT3 and TRAb are the risk factors of HHD. Timely prevention and control of risk factors is necessary to reduce the incidence of HHD.

Objective To screen the arsenic content situation of drinking water in lower reaches of Yellow River and survey the amount of threatened people drinking high arsenic water and the condition of endemic arsenism.Methods Four counties of Yuncheng,Jiaxiang,Dongchangfu and Boxing were selected to colleft the water samples by CroOSS-sectional survey method.The water arsenic content wag determined by semi-quantitative rapid kit.All water samples having arsenic were re-determined by atomic fluorescence spectrometry.And the nurober of threatened people who drinking high arsenic water were investigated.Results In 4765 water wells screened,303 water samples had contained arsenic,arsenic content of 35 samples Was≥0.030 mg/L,12 samples were exceeding the international standard (arsenic content≥0.050 ms/L),they distributed in 3 counties of Dongchangfu,Yuncheng and Jiaxiang.The residents drinking water wells of arsenic content≥0.030 mg/L were surveyed by epidemiological investigation.And in the 28 villages 13 032 residents and 11 Bu8picious patient8 wlere checked out.Conclusion The wells with excesive water arsenic content are existing in the lower reaches of Yellow River and people suspicious of endemic arsenism need to be further identified.

To explore the role of regulatory peptides in the secretion of bronchial epithelial cells (BECs), we observed the effects of four peptides, i.e.vasoactive intestinal peptide (VIP), epidermal growth factor (EGF), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP), on the secretion of ILs from unstimulated or O3-stressed BECs. The results of the experiments showed that VIP exerted an inhibitory effect on the secretion of IL-1 and IL-8 from unstimulated and O3-stressed BECs, VIP also decreased the secretion of IL-5 from O3-stressed BECs; EGF promoted secretion of IL-1 and IL-8 from unstimulated BECs, but decreased the secretion of ILs from O3-stressed BECs; ET-1 and CGRP enhanced the secretion of IL-1, IL-5, and IL-8 from unstimlated BECs, CGRP also increased the secretion of ILs from O3-stressed BECs. The results obtained demonstrate that intrapulmonary regulatory peptides modulate the secretion of ILs from BECs, and may play an important part in transduction of inflammatory signals.
Animals ; Bronchi ; cytology ; Calcitonin Gene-Related Peptide ; pharmacology ; Cells, Cultured ; Endothelin-1 ; pharmacology ; Epidermal Growth Factor ; pharmacology ; Epithelial Cells ; drug effects ; secretion ; Female ; Interleukins ; secretion ; Male ; Rabbits ; Vasoactive Intestinal Peptide ; pharmacology

To detect levels of platelet glycoprotein-specific autoantibody in idiopathic thrombocytopenic purpura (ITP), chronic aplastic anemia (CAA), hematologic malignancies and healthy volunteers, and evaluate the clinical significance of platelet glycoprotein-specific autoantibody level in diagnosis for ITP, anti-GPIb/IX, anti-GPIIb/IIIa, anti-GPIV and anti-GPV auto-antibodies were detected contemporaneously by a modified monoclonal antibody immobilization of platelet antigen assay (modified MAIPA). The results showed that the total positive rate of antibodies against platelet GPIb/IX, GPIIb/IIIa, GPIV, GPV were 69.99%, 10%, 20% and 0% in ITP, CAA, hematologic malignancy group and healthy volunteers respectively. There was significant difference between ITP and CAA (chi(2) = 20.71, P < 0.005), between ITP and hematologic malignancy group (chi(2) = 12.22, P < 0. 005). There was no positive finding in the healthy control. It is concluded that platelet glycoprotein-specific autoantibody has high value for the diagnosis of ITP,many kinds of antibodies detection at one time can enhance sensitivity, MAIPA is a specific assay for the diagnosis of idiopathic thrombocytopenic purpura.
Adolescent ; Adult ; Aged ; Autoantibodies ; blood ; Child ; Female ; Humans ; Male ; Middle Aged ; Platelet Membrane Glycoproteins ; immunology ; Purpura, Thrombocytopenic, Idiopathic ; diagnosis ; immunology ; Sensitivity and Specificity

OBJECTIVETo construct a modified and enhanced thymidine kinase (TK) vector regulated by human telomerase catalytic subunit promoter (hTERT) promoter and cytomegalovirus (CMV) enhancer and its killing effect on nasopharyngeal carcinoma in vitro and in vivo and its safety in vivo.

METHODSThe pGL3-basic, as basic vector template, was linked and constructed into TK vector regulated by hTERT promoter and CMV enhancer with mono-promoter vector as control. Enhanced TK expression was confirmed by fluorescent microscopy and real time fluorescent quantitative PCR. Telomerase activity was measured by stretch PCR. Tumour killing effects were examined by MTT and Boyden areola. The effects of enhanced TK on the invasiveness of tumor cell NPC 5-8F and the growth of xenograft implanted in nude mice were investigated.

RESULTSCompared with non-enhanced vector, TK expressed by the enhanced vector significantly increased in NPC 5-8F and MCF-7 cells, telomerase activity was positive in human in NPC 5-8F cells and breast cancer MCF-7 cells and negative in control human blood vessel endothelium ECV-304 cells. After ganciclovir(GCV) treatment, NPC 5-8F cell survival rate and invasiveness decreased and tumor progress of NPC xenograft implanted in nude mice was inhibited, without obvious toxicity effects on mouse liver and kidney.

CONCLUSIONSThe enhanced TK vector regulated by hTERT promoter and CMV enhancer can obviously and specifically inhibit and kill nasopharyngeal carcinoma cells in culture and nasopharyngeal carcinoma xenograft in nude mice in vivo, without obviously toxic side effects on nude mice. The targeted and enhanced TK gene vector with high performance may be a new tumour targeted gene therapy strategy clinically to aim directly at most malignant tumours including nasopharyngeal carcinoma, with more extensive anti-cancer spectrum.

Animals ; Cell Line, Tumor ; Cell Survival ; Cytomegalovirus ; genetics ; Genetic Vectors ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Nasopharyngeal Neoplasms ; therapy ; Promoter Regions, Genetic ; Regulatory Sequences, Nucleic Acid ; Telomerase ; genetics ; Thymidine Kinase ; genetics ; Transfection