Objective: To construct a lentiviral vector of RNA interference(RNAi) of TGF-?1 gene. Methods: Three target sequences were selected according to TGF?1 mRNA sequence firstly,then three pairs of oligonucleotide sequences according to these target sequences and one pair of negative control oligonucleotide sequence were designed and synthesized. The annealed oligonucleotide fragments were subcloned into pRNA-U6/Lenti plasmid. After identified by restriction enzyme digestion and sequencing ,these plasmids were cotransfected along with ViraPowerTM Lentiviral Packaging Mix into 293FT cells for the package of lentiviral particles. Then the lentiviral vector particles were transfected into Hela cells and TGF-?1 expression in the transfected cells was assayed by Real-Time PCR and ELISA. Results: It was confirmed by digestion and sequencing that lentiviral vectors had the correct structure and could express high titer of virus. After transfected into Hela cells,TGF-?1 expression was decreased significantly by two of these lentiviral vectors at both the protein and mRNA level and the first vector had the best interefence efficiency. Conclusion: The lentivirus RNAi vector of TGF-?1 was constructed successfully.

Intervention measures of a nursing student with hepatitis B carrier and situational mental disorder are reported,including inducing emotional release,helping nursing students appropriately recognize and deal with diseases,and searching effective social and family support.Related issues are also reflected on these interventions,such as to implement in-time mental nursing,stress hepatitis B related health education,deal with the conflict and balance between right of privacy and informed consent,and widespread related laws.

Objective To explore the application value of blood lipid,blood glucose,liver function and serum uric acid in the diagnosis of fatty liver in elderly patients.Methods A total of 62 elderly patients with fatty liver in our hospital from April 2016 to October 2016 were selected as study group,and 62 healthy persons were selected as control group.All the subjects were drawn fasting venous blood 4 mL.Rate method was used to detected liver function indexes of total bile acid(TBA),gamma glutamyltranspeptidase(GGT) and aspartate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL) levels.The hexokinase method was used to detected blood lipid indexes,including very low density lipoprotein cholesterol(VLDL-C),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),cholesterol(TC),triglyceride(TG) levels.The peroxidase colorimetric method was used to detected blood glucose index including glycated hemoglobin(HbA1c) and fasting blood glucose(FBG) levels.MINDRAY BS-400 automatic biochemical analyzer produced in Shenzhen MINDRAY company was used to detect the serum uric acid level.Value of blood lipid,blood glucose,liver function,serum uric acid in diagnosis for fatty liver were made statistical comparison.Results The levels of TBA,GGT,AST,ALT and TBIL in the study group were higher than those in the control group,the level of VLDL-C,LDL-C,TG,TC in the study group were higher than those in the control group,the HDL-C level was lower than that in the control group,the levels of FBG and HbA1c in the study group were higher than those in the control group,the level of serum uric acid in the study group[(447.12±91.03)μmol/L] was higher than that in the control group [(353.41±70.32)μmol/L],the differences were all had statistically significant(P<0.05).The sensitivity(80.65%,79.03%,82.26%,77.42%) and accuracy(90.32%,88.71%,90.32%,88.71%) of blood lipids,blood glucose,liver function,blood uric acid alone in diagnosis of fatty liver were lower than the combined diagnosis(95.16%,96.77%),the differences were statistically significant(P<0.05).Conclusion Fatty liver patients have abnormal blood lipids and blood glucose,liver function and blood uric acid level,combined with the above indexes for diagnosis can improve the sensitivity and accuracy of fatty liver,and provide guidance for the intervention,is worthy of promotion.

Aged ; Aged, 80 and over ; Humans ; Leukemia, Myeloid, Acute

Objective To summarize the strategies of nutritional support for patients with hyperlipidemic acute pancreatitis (HLAP). Methods The clinical data of six HLAP patients who were treated in our department from January 1998 to December 2009 were retrospectively reviewed. Results The disease conditions of all patients were improved after fasting and early nutritional support and lipid-lowering treatment. Three cases with severe HLAP received enteral nutrition via a nasojejunal tube. Another three patients with milder HLAP obtained low-fat diet and orally administered lipid-lowering drugs. The plasma triglyceride levels decreased from (25.30 ± 20. 48 ) mmol/L to (5.41 ±4.55) mmol/L. No relapse was noted during follow-up (range: 3 -24 months). Conclusions The approaches of nutritional support for HLAP patients should be customized based on the specific disease conditions,and the enteral nutrition and lipid-lowering drugs should be reasonably and carefully designed and applied. For severe HLAP, nasojejunal tube should be maintained in the early stage to ensure the application of enteral nutrition and lipid-lowering drugs, protect the integrity of gut mucosal barrier, and decrease the fluctuation of blood glucose.

Even though chemistry is now in place that potentially allows high coupling efficiencies to be attained, successful coupling is usually a challenge when so-called "difficult sequences" is encountered in peptide synthesis. Some factors that affect the coupling efficiency have been discussed and related methods to overcome those obstacles have been introduced in present review. All suggestions proposed here are valuable and also feasible to improve the coupling completeness in both liquid-phase or solid-phase peptide synthesis.

Acute Disease ; Adult ; China ; Humans ; Leukemia, Myeloid ; therapy

Humans ; Leukemia ; Neoplastic Stem Cells

Objective To evaluate the efficacy and safety of XELOX chemotherapy combined with Chinese herbal medicines in patients with obstructive colorectal cancer.MethodsA total of 70 patients with obstructive colorectal cancer were recruited and divided into a XELOX treatment group and a combined treatment group, 35 in each group. The XELOX treatment group received XELOX chemotherapy, while the combined treatment group were received XELOX chemotherapy combined with Chinese herbal medicines. All patients received a 3-week treatment as a cycle, and for 4 cycles. The outcomes were Karnofsky performance status (KPS) and the Zubrod-ECOG-WHO Scale (ZPS). Adverse effects were recorded.ResultsAfter the treatment, the clinical symptoms score (1.62 ± 0.21vs. 2.12 ± 0.31;t=7.900,P<0.01) and ZPS score (1.07 ± 0.33 vs. 2.88 ± 0.41;t=20.346,P<0.01) in the combined treatment group were significantly lower than those in the XELOX treatment group, but the KPS score was significantly higher (93.42 ± 5.56vs. 86.43 ± 5.93;t=5.087, P<0.01). The follow-up showed that the 3-year survival rates in the combined treatment group was significantly lower than those in the XELOX treatment group (80.00%vs. 57.14%;χ2=4.242,P=0.039). The incidence of leukopenia (11.43%vs. 34.29%;χ2=3.970,P<0.05), anemia (5.71%vs. 22.86%;χ2=4.200,P<0.05), nausea/vomiting (8.57%vs. 28.57%;χ2=4.629,P<0.05), and diarrhea (2.86%vs. 20.00%;χ2=5.081,P<0.05) in the combined treatment group were significantly lower than those in the XELOX treatment group. Conclusion XELOX chemotherapy combined with Chinese herbal medicines can improve clinical symptoms, performance status, and alleviate adverse effects in patients with obstructive colorectal cancer.

OBJECTIVE:To investigate the revenue and expenditure in PIVAS of our hospital,and to provide data for the es-tablishment and operation of PIVAS in other hospitals. METHODS:A statistics was undertaken for the revenue and expenditure of PIVAS in 2014,and partial influential factors were analyzed comprehensively. RESULTS:In 2014,under the premise of only long-term doctor's advice for the morning,760611 groups of intravenouse drugs had been administered in PIVAS of our hospital. The revenue was about 2197439 yuan and the expenditure was about 1872598 yuan;the cost of human resource was the main cost part,which took 77.92%. CONCLUSIONS:The revenue of PIVAS almost covers the cost of pharmacy in our hospital at pres-ent;the data of cost-effectiveness can be reference for other hospitals.