Objective To investigate the morphologic changes and hemodynamic parameters of the common carotid artery in obstructive sleep apnea hypopnea syndrome(OSAHS)in a porcine model using ultrasound and color Doppler.Methods A total of 12 pigs were randomly divided into model group and control group(n=6 for each).All pigs were fed under the same conditions with exception of th ose in the model group which were kept in a hypobaric chamber for experiments.The parameters of systolic velocity(S)and diastolic velocity(D),resistant index(RI)and S/D in Doppler as well as maximum IMT(intima-media thickness)with two-dimensional ultrasound were obtained in the carotid artery of all animals were determined,and the latter was confirmed by animal dissection when the symptoms of OSAHS appeared in the model group.Results The diameter of the lumen of the common carotid artery in model group(3.18?0.17)was smaller than that of control group(3.47?0.15,P

Objective To investigate the association between single nucleotide polymorphisms (SNP) rs 653765 and rs514049 inADAM10gene and clinical characteristics in asthma in Han Chinese children. MethodsA case-control study was performed in that research, and 221 children with asthma were recruited and an other 236 normal children were recruited as controls. The genotypes of two SNPs in ADAM 10 gene were detected using PCR-RFLP. And all data were analyzed by SPSS 19 . 0 . Results In case-controlled study, we found the frequency of TT genotype in case group was higher than that of controlgroup (P=0 . 028 ), and the frequency of T allele was higher than that of control group (P=0 . 008 ). The genotypes and alleles of rs 514049 was not associated with asthma (P=0 . 604 , 0 . 356 ).There were no difference in serum ADAM 10 levels between asthma and control group (P=0 . 238 ), but in asthma group we found patients with TT genotype may have a higher level ADAM 10 than the one with CC genotype (P=0 . 034 ). The polymorphism of rs 653765 was not associated with the level of C-reaction protein, the number of LYM, IgE and EOS% (P>0 . 05 ). Conclusions The genotype of rs 653765 in ADAM 10 gene was associated with asthma in the central of China, and T allele was a risk factor. The polymorphism of rs 653765 might be associated with the levels of ADAM 10 .

Objective To evaluate the diagnostic value of endoscopic ultrasonic elastography in lymph node staging of gastrointestinal tumors.Methods Thirty-nine abdominal lymph nodes and 26 mediastinal lymph nodes of 35 patients with gastric cancer or esophageal carcer were examined by endoscopic ultrasonic elastography of five-point elastic score.Lymph node at 1-3 points was regarded as negative(benign),while 4-5 points as positive(malignant).The diagnostic value of EUS elastography was determined by comparing the results with pathological findings.Results Of all the 65 lymph nodes,49 were diagnosed as positive and 16 negative.The sensitivity and specificity of EUSE in the diagnosis of malignant lymph nodes were 91.1%and 60.0%.respectively.Conclusion EUSE is helpful for diagnosis of malignant metastasic lymph nodes.

Administration, Oral ; Adult ; Antioxidants ; administration & dosage ; pharmacology ; Ascorbic Acid ; administration & dosage ; pharmacology ; Humans ; Lipid Peroxidation ; drug effects ; Male ; Middle Aged ; Occupational Health ; Vitamin E ; administration & dosage ; pharmacology

Objective To investigate the changes of interleukin(IL)-2 and interferon(IFN)-γin serum and synovial fluid of patients with joint injure with Kashin-Beck disease(KBD), and study the roles of IL-2 and IFN-γ in KBD joint injure. Methods In accordance with the "Diagnostic Criteria of Kashin-Beck disease"(GB16003-1995),48 cases of KBD patients and 26 healthy people(control group) from KBD endemic area in Long county Shaanxi province were enrolled in the study. KBD patient were 24 males and 24 females, respectively, aged 40 to 65 years (mean age 51 years). Forty-eight serum specimens and 28 synovial fluid specimens of patients(14 males and 14 females,respectively) were collected. Healthy control group were 13 males and 13 females, respectively. Twenty-six serum specimens of healthy controls were collected. Serum and synovial fluid IL-2 and IFN-γ levels were determined by enzyme-linked immunosorbent assay(ELISA). Results In healthy controls and KBD patients, the midian of serum IL-2 were 46.8 ng/L and 55.7 ng/L, respectively, and IFN-γ were 52.3 ng/L and 48.8 ng/L, respectively. The difference was not statistically significant between healthy controls and KBD patients(t = 0.62, 0.70, all P ＞ 0.05).In synovial fluid of KBD patient, the midian of IL-2 and IFN-γwere 48.3 ng/L and 44.1 ng/L, respectively. The difference was not statistically significant between serum and synovial fluid in KBD patients(t = 0.69, 1.72, all P ＞0.05). Conclusion Serum and synovial fluid IL-2 and IFN-γare not significantly increased in KBD patients with articular damage, indicating that IL-2 and IFN-γare not involved in KBD joint injury.

Animals ; Electrical Synapses ; Male ; Membrane Potentials ; physiology ; Neurons ; physiology ; Parietal Lobe ; cytology ; Patch-Clamp Techniques ; Rats ; Rats, Wistar

Chromatography, Gas ; methods ; Chromatography, High Pressure Liquid ; methods ; Humans ; Hypnotics and Sedatives ; blood ; Pentobarbital ; blood ; Pharmaceutical Preparations ; blood ; Sensitivity and Specificity

The purpose of this study is to obtain effective and stable bacteriocin-like substance from lactic acid bacteria. Lactobacillus buchneri KLDS1.0364,which was isolated from fermented cream,a traditional dairy product in Inner Mongolia,could produce bacteriocin-like substance. The bacteriocin-like substance produced by KLDS1.0364 was partially purified and the characteristics were studied. The bacteriocin-like substance was purified by SP-Sepharose fast flow cation exchange chromatography. The molecular weight of the bacteriocin-like substance was 21.6kD,as determined by tricine-SDS-PAGE. The bacteriocin-like substance remained stable after 30 min at 121℃ and after 2 h of incubation during pH 2～10. Complete inactivation of antimicrobial activity was observed after treatment of the bacteriocin-like substance with several proteinases. Treatment with catalase and ?-amylase did not result in any changes of antimicrobial activity. The mode of action of the bacteriocin-like substance is bactericidal. It exhibited a broad spectrum of antagonistic activity against various species of Gram-positive bacteria,Gram-negative bacteria and fungi.

In order to study the interaction relationship between secondary metabolites in Glycyrrhiza uralensis, and find out which secondary metabolite is significantly related to the content of glycyrrhizic acid, artificial applying ammonium glycyrrhetate solution was used to establish a high glycyrrhizic acid environment. The change of the 4 secondary metabolites was analyzed within 72 h after glycyrrhizic acid stimulation, while correlation statistical soft was applied to analyze the correlation of glycyrrhizic acid and other compositions. It turned out that it is feasible to establish high glycyrrhizic acid environment by glycyrrhizic acid root soaking in the concentration of 1.0 mmol x L(-1). There was significant positive correlation between glycyrrhizic acid and liquorice glycosides in short-term glycyrrhizic acid stimulation environment. It is concluded that glycyrrhizic acid accumulation internal of G. uralensis could be effected by artificial exogenous glycyrrhizic acid stimulation in certain case, and its accumulation was significantly related to the content of liquorice glycosides.
Glycyrrhiza uralensis ; chemistry ; drug effects ; metabolism ; Glycyrrhizic Acid ; analysis ; metabolism ; pharmacology ; Plant Roots ; chemistry ; drug effects ; metabolism ; Secondary Metabolism ; drug effects ; Time Factors

An experiment was conducted using cultivated Glycyrrhiza uralensis in age of one year to study the effects of abscisic acid (ABA) on chemical components content and color of G. uralensis. By using different concentrations of ABA spraying on leaves, the change of the chemical component content was analyzed within 45 d after ABA stimulation, and the effects on quality were studied combined with colorimetric analysis data. It turned out that in some sense the content of glycyrrhizic acid and liquiritin had increased within 45 d, especially for liquiritin. After high concentrations of ABA (3.96 mg · L(-1)) stimulating, the content of glycyrrhizic acid rose 52% while liquiritin up 392% within 30 d. Then they both showed a decline in the content of glycyrrhizic acid and liquiritin on 45 d. Color index values of a* and b* were all significantly higher than that of the control group within 45 d, which meant the color of powders turned toward red and yellow. The conclusion was that ABA (3.96 mg · L(-1)) stimulating could not only improve the quality in the traditional sense through the color of G. uralensis, but also in the modern sense by improving the content of glycyrrhizic acid and liquiritin.
Abscisic Acid ; pharmacology ; Color ; Drugs, Chinese Herbal ; chemistry ; Flavanones ; analysis ; Glucosides ; analysis ; Glycyrrhiza uralensis ; chemistry ; drug effects ; growth & development ; Glycyrrhizic Acid ; analysis ; Plant Growth Regulators ; pharmacology