Objective: To develop an HPLC-DAD method for the simultaneous determination of five active flavonoids (quercetin, kaempferol, apigenin, 3-methoxyl-quercetin, nobiletin) in Centipeda minima (L.) A.Br.et Aschers.Methods: The chromatographic separation was performed on a Diamonsil C18 column (200 mm×4.6 mm,5 μm) with the mobile phase of 0.1% phosphoric acid-acetonitrile with gradient elution at the flow rate of 0.8 ml·min-1.The detection wavelength was set at 360nm,and the column temperature was maintained at 30 ℃.Results: Quercetin, kaempferol, 3-methoxyl-quercetin, apigenin and nobiletin was linear within the range of 0.002 3-0.093 0 μg·μl-1(r=0.999 5) , 0.002 2-0.087 0 μg·μl-1(r=0.999 6),0.002 0-0.079 0 μg·μl-1(r=0.999 8), 0.000 9-0.037 0 μg·μl-1(r=0.999 8) and 0.000 8-0.031 0 μg·μl-1 (r=0.999 9), respectively.The average recovery was 97.66%(RSD=1.17%), 98.33%(RSD=1.16%), 98.63%(RSD=1.10%), 98.40%(RSD=1.52%) and 98.10%(RSD=1.36%)(n=6) , respectively.Conclusion: The method is convenient, accurate and reproducible, which can be used for the quality control of Centipeda minima (L.) A.Br.et Aschers.

BACKGROUND: Pathogenesy of immunoglobulin A nephropathy (IgAN) is not clear up to now. Present research has verified that the key pathogenetic pathway is abnormalities of IgA1 molecular O-glycosylation induced by decrease of ?1,3-galactosyltranferase activity in IgA1 hinge region of IgAN patients. Prophase study by the authors supposed that the key of IgAN O-glycosylation abnormality might be due to the decrease of ?1,3-galactosyltranferase specific molecular protein chaperone Cosmc in B lymphocyte of peripheral blood in IgAN patients. OBJECTIVE: To measure DNA sequence of ?1,3-galactosyltranferase specific molecular chaperone in coding region of Cosmc gene in IgAN patients,and compared with the sequence of Gene Bank. DESIGN: Case-controlled observation. SETTING: Department of Nephrology,West China Hospital,Sichuan University. PARTICIPANTS: Totally 27 IgAN patients and 10 non-IgAN patients were recruited in Department of Nephrology of West China Hospital of Sichuan University from November 2005 to August 2006,and five normal controls were included in this study. All the subjects knew the fact and agreed to participate in the experiment. METHODS: The experiment was performed at the State Key Laboratory of Biotherapy of Sichuan University. 2 mL peripheral venous blood of all the samples were taken into heparin sodium anticoagulated tubes,from which total genomic DNA were extracted by phenol/chloroform precipitation method. Concentration of DNA was determined by ultraviolet spectrophotometer. The polymerase chain reaction (PCR) was used to amplify the coding region of ?1,3-galactosyltranferase specific molecular chaperone Cosmc gene in all the subjects and direct sequencing was done in PCR products of each subjects. The results of all the sequencing were compared with Gene Bank one by one. MAIN OUTCOME MEASURES: Amplification findings and sequencing of coding region of ?1,3-galactosyltranferase specific molecular chaperone Cosmc gene by PCR. RESULTS: ①Coding region of Cosmc gene located at 257-1 213,and amplified Cosmc gene was 1 247 bp. ②The sequence of Cosmc gene coding region was similar in IgAN patients,non-IgAN patients and normal controls,and no difference of gene sequence was noticed in all the result sequences as compared with the Gene Bank registered sequence. CONCLUSION: No abnormal sequence is found in coding region of Cosmc gene in IgAN patients,suggesting that this coding region probably is not associated with the abnormalities of IgA1 O-glycosylation in IgAN.

Childhood acute lymphoblastic leukemia (ALL) is a kind of abnormal proliferation of malignant tumor diseases originated in lymphocytes,which is the most common malignant tumor in children.Although stratified treatment has significantly improved the efficacy of ALL in children,but 15％-20％ of the patients still have ultimately relapsed due to the minimal residual disease(MRD).MRD refers to the leukemia patients after induction chemotherapy complete remission (or bone marrow transplant),in vivo residual morphology which could not be detected in trace amounts of leukemia cells.The detection methods of MRD mainly include flow cytometry,quantitative real-time polymerase chain reaction and immune repertoire sequencing.MRD level detection is of great important to judge the prognosis,and to guide the risk grouping and individual treatment,and so on.Now,the progress in the clinical application of MRD in children with ALL in recent years was reviewed.

Objective To investigate the effect of cardioplegia solution of autologous blood treated with ultraviolet irradiation and oxygenation (UBIO) on myocardial mitochondrion in mongrel dogs underwent open heart surgery under cardiopulmonary bypass (CPB).Methods Twenty male mongrel dogs were randomly divided into a control group and a UBIO group, 10 dogs in each group.The UBIO group was infused with UBIO blood as the cardiac arresting solution via the ascending aorta, while the control group was given the same treatment except that the cardiac arresting solution was blood cardioplegia.Blood samples were taken from coronary venous sinus before cross-clamping and after aorta declamping to measure the levels of cardiac troponin I (cTnI) and creatinine kinase MB isozyme (CK-MB).For both groups, right atrial myocardial tissue samples were taken to detect the activity of myocardial mitochondrion superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) , and concentrations of malondialdehyde (MDA) when the right atrium was opened and closed.Results In both groups, the average levels of SOD and GSH-PX were decreased during CPB, and the decrease of SOD and GSH-PX were to a significantly larger extent in UBIO group (P ＜ 0.05).The concentrations of cTnI, CK-MB, and MDA in the control group were increased significantly higher than those in the UBIO group (P ＜ 0.05).Conclusion The attenuated lipid peroxidation of myocardial mitochondria plays an important role in myocardial protection by UBIO as cardioplegia solution in open heart surgery under CPB.

Humans ; Male ; Mesylates ; poisoning ; Occupational Diseases ; Occupational Exposure ; adverse effects ; Young Adult

Child ; Diabetes Mellitus, Type 1 ; genetics ; Female ; Humans ; Infant ; Male ; Siblings

Objective To explore the value of Montreal Cognitive Assessment (MoCA) in the diagnosis of mild cognitive impairment. Methods Mini-mental state examination (MMSE) was used in 532 elderly persons aged 60 years and over in Xuzhou city Gulou county. The 69 healthy people and patients with mild cognitive impairment were chosen to undergo MoCA. Then the sensitivity and specificity of MoCA were analyzed. Results According to MMSE, there were 19 patients with mild cognitive impairment (27. 5 %), 50 healthy persons (72.5 %). While according to MoCA, there were 58 patients with mild cognitive impairment (84.1%), 11 healthy persons (15.9 %). The consistency of the two scales was not good. And compared with MMSE, the sensitivity of MoCA was 94.7%, and the specificity was 20. 1%. Conclusions In the diagnosis of mild cognitive impairment, MoCA is more sensitive than MMSE.

BACKGROUND: Pathogenesy of immunoglobulin A nephropathy (IgAN) is not clear up to now. Present research has verified that the key pathogenetic pathway is abnormalities of IgA1 molecular O-glycosylation induced by decrease of β1, 3-galactosyltranferase activity in IgA1 hinge region of IgAN patients. Prophase study by the authors supposed that the key of IgAN O-glycosylation abnormality might be due to the decrease of β1, 3-galactosyltranferase specific molecular protein chaperone Cosmc in B lymphocyte of peripheral blood in IgAN patients.OBJECTIVE: To measure DNA sequence of β1, 3-galactosyltranferase specific molecular chaperone in coding region of Cosmc gene in IgAN patients, and compared with the sequence of Gene Bank.DESIGN: Case-controlled observation.SETTING: Department of Nephrology, West China Hospital, Sichuan University.PARTICIPANTS: Totally 27 IgAN patients and 10 non-IgAN patients were recruited in Department of Nephrology of West China Hospital of Sichuan University from November 2005 to August 2006, and five normal controls were included in this study. All the subjects knew the fact and agreed to participate in the experiment.METHODS: The experiment was performed at the State Key Laboratory of Biotherapy of Sichuan University. 2 mL peripheral venous blood of all the samples were taken into heparin sodium anticoagulated tubes, from which total genomic DNA were extracted by phenol/chloroform precipitation method. Concentration of DNA was determined by ultraviolet spectrophotometer. The polymerase chain reaction (PCR) was used to amplify the coding region of β1, 3-galactosyltranferase specific molecular chaperone Cosmc gene in all the subjects and direct sequencing was done in PCR products of each subjects. The results of all the sequencing were compared with Gene Bank one by one.MAIN OUTCOME MEASURES: Amplification findings and sequencing of coding region of β1, 3-galactosyltranferase specific molecular chaperone Cosmc gene by PCR.RESULTS: ①Coding region of Cosmc gene located at 257-1 213, and amplified Cosmc gene was 1 247 bp. ②The sequence of Cosmc gene coding region was similar in IgAN patients, non-IgAN patients and normal controls, and no difference of gene sequence was noticed in all the result sequences as compared with the Gene Bank registered sequence.CONCLUSION: No abnormal sequence is found in coding region of Cosmc gene in IgAN patients, suggesting that this coding region probably is not associated with the abnormalities of IgA1 O-glycosylation in IgAN.

ObjectiveTo investigate the clinical and electrophysiological features of chronic inflammatory demyelinating polyneuropathy (CIDP) . MethodsThe clinical symptoms and signs of 11 patients with CIDP were studied, motor conduction velocity( MCV), sensory con-duction velocity (SCV) and Electromyography (EMG) were also respectively carried out on 54 motor nerves, 28 sensory nerves and 21 musclesof these 11 cases. The amplitudes of compound muscle action potential(CAMP) obtained from distal and proximal ends were compared to as-certain the presence of conduction block(CB) by stimulating the segments starting from the distal ends. ResultsMore than 3 nerves werefound involved in 10 out of 11 cases, slow MCV were found in 52%, prolongation of the distal latency in 64%, reduction of the amplitudes ofCAMP in 68%,CB in 26%,slow SCV in 85. 7%. EMG revealed neurogenic damage in 81%. ConclusionCIDP is a peripheral de- myelinating neuropathy involving not only the prox imal and distal segments but also the sensory and motor nerves. If there were no conditionsto perform nerve biopsy, testing of protein in CSF and electrophysiology mightbe of important diagnostic value for CIDP.

OBJECTIVE: To promote the development of clinical pharmacy and improve the social efficacy of ADR monitoring so as to ensure safe and rational use of drugs.METHODS: The current status of Chinese ADR monitoring system and the problems faced in the development of ADR monitoring were analyzed by means of literature review,contrasting analysis,and data reduction etc.RESULTS & CONCLUSION: There are many problems existed in the current ADR monitoring,for instance: the regulations system of ADR monitoring is unsound,the monitoring technology is weak;the cognition level of many medical workers is still very low etc.It is urgently needed to intensify propaganda on ADR monitoring,strengthen ADR monitoring,develop technological system of monitoring,set up expert database and train professional talents.