ObjectiveTo determine the diurnal variation,moon phase,and seasonal variation in the onset of stroke. MethodsThe time parameters,including the time,the moon phase and the season of the stroke occurrence for 905 in-patients from 1989-2001 were investigated.ResultsThere was a significant difference (D=0.155,P<0.01) for circadian rhythm in the onset of 321 cases with cerebral infarction,and the incidence frequency at dawn was much higher than that at other time,but the difference of the moon phase and seasonal rhythm in the onset of cerebral infarction were not significant(P>0.05).There were not diurnal variation,moon phase and seasonal rhythms in the onset for cerebral haemorrhage(P>0.05).ConclusionsThe cerebral infarction occur more frequently at dawn time.

OBJECTIVE:To observe the clinical efficacy and safety of cinepazide maleate combined with oxiracetam in the treat-ment of vascular dementia. METHODS:62 patients with vascular dementia were randomly divided into treatment group and control group,with 31 patients in each group. Patients in control group were treated with Oxiracetam injection 4g/d for 3weeks. Patients in treat-ment group were treated with cinepazide maleate 160 mg/d for 3 weeks on the basis of control group. The patients of 2 groups were exam-ined with Mini-mental State Examination (MMSE)and Activity of Daily Living Scale (ADL)before and after treatment. RESULTS:There was statistically significant difference in MMSE and ADL scores of 2 groups before and after treatment(P<0.05);MMSE score and ADL score of treatment group was better than that of control group,with statistical significance(P<0.05);Obvious ADR was not found in both groups. CONCLUSIONS:Cinepazide maleate combined with oxiracetam can significantly improve ADL and MMSE score in patients with vascular dementia,improve the quality of life,showing significant and good clinical efficacy.

Tumor marker is a hot subject of research in oncology field,which would promote the diagnosis and treatment in clinic.Tumor markers of children's germ cell tumor include α-fetoprotein,glypican 3,SALL4,glial cell line-derived neurotropic factor receptorα-1(GFRα-1),Ki-67,octamer-binding transcription factor4,CD117,D2-40 and CD30.SALL4 would be used as a new tumor marker for germ cell tumor,specially for yolk sac tumor.GFRα-1 can be helpful for the diagnosis of immature teratoma.

ObjectiveTo study the effect of Xiangdan point injection in upper limb spasticity after stroke. Methods56 cases were divided into two groups: 28 cases for treatment group were treated with point injection Xiangdan, combined with routine therapy. Another 28 cases for control were only routinely therapy. ResultsThe cure rate of the treatment group was 29% ,the effective rates was 100%, while that of control group was 11% and 25% respectively (P<0.01).Conclusions Xiangdan point injection is more effective in upper limb spasticity after stroke than only routine therapy.

112 cases with hemiplegia were randomly divided into three groups,training,acupuncture,and integrated training and acupuncture.Their effects were observed for three monthes.Results:the effect of the integrated training and acupuncture group was significantly better than the other two groups(P＜0.01).The results comfirmed that the motor function of the hemiplegic limbs was enhanced effectively with the integrated training and acupuncture.

Objective To investigate the genetic characteristics of viruses associated with viral diarrhea in Jilin Province, in order to provide a scientific basis for making more effective prevention and control strategies of viral diarrhea.Methods A total of 211 fecal specimens from hospitalized children under 5 years of age with diarrhea and 7 sewage samples were collected in Jilin Province from January to July 2023. Initial screening was performed using qPCR, and positive specimens were further analyzed by RT-PCR to amplify target genes. Sequencing was conducted, and phylogenetic trees were constructed to analyze genetic relationships.Results In fecal specimens, the positive rates for rotavirus(RV), Norovirus(NoV),Sapovirus(SaV), astrovirus(AstV), and adenovirus(AdV) were 10. 9%, 48. 3%, 4. 3%, 1. 4%, and 5. 7%, respectively. In sewage samples, 5 AstV and 4 NoV were detected, along with 3 samples each of RV, SaV, and AdV. Sequencing and genotyping results revealed that RV G8P [8] was the predominant circulating strain. Among NoV strains, GⅡ.4 [P16] was the most prevalent, followed by GⅡ.4 [P31]. In fecal samples, GⅠ group strains included GⅠ.3 [P13] and GⅠ.1 [P1]. One SaV strain of GⅡ.5 was identified. For AstV, three strains of HAstV-1a and one strain of HAstV-5 were detected. Among AdV strains, three were identified as HAdV-41, three as HAdV-2, one as HAdV-1, and one as HAdV-5.Conclusion The pathogenic genes of viral diarrhea in Jilin Province are diverse, the dominant strains of RV have changed, and domestic sewage contains a variety of diarrhea viruses.

OBJECTIVE:To develop the data processing program of Digoxin blood drug concentration(DBDP)and to determine the range of effective blood concentration of Digoxin.METHODS:The DBDP program was developed by VISUAL BASIC6.0and run in Windows95system.The data of blood drug concentration,which was determined with flurescence polarization immunoassay(FPIA)in our hospital,was analyzed with DBDP program and effective blood drug concentration range of Digoxin was set up.RESULTS:The DBDP program integrated functions including pharmacokinetic parameter estimation,establishment of the effective blood drug concentration range,management of the data of the blood drug concentration moni?toring,automatic addition of patient's file,multifunctional fuzzy searching,retrospective analysis and clinical query about drug use.The effective blood drug concentration range of Digoxin was0.58～2.01ng/ml.CONCLUSION:The DBDP program is a very practical tool for the development of therapeutic drug monitoring and it is very necessary to develop blood drug concen?tration monitoring and establish the effective blood drug concentration range.

AIM: To investigate whether berberine can ind uce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to di fferentiate by berberine. The morphological changes of MSCs were evaluated by li g ht microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by be rberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differe ntiate into neuron-like cells in vitro.

OBJECTIVE :To give sonic references for improving the quality of drug use and the regulations of pharmacy in China. METHODS: In this paper. OBRA - 90 and the situation of drug use review in America are introduced and analyzed,and inadequate insurance in drug use in China is pointed out.RESULTS & CONCLUSION :The drug use review system in USA is quite successful in ensuring the safety of drug use in public, and we can learn something from it to improve the quality of medication in China.

BACKGROUND:Anterior cervical discectomy and bone graft fusion are effective method to treat cervical spondylosis. This method can provide firm fixation and the fusion rate is high. However, for cervical vertebra involved in more than two segments, the stability of fixator and bone graft is poor due to big bone graft span, which may easily cause fusion failure and pseudarthrosis, and impact curative effects. OBJECTIVE:To investigate biomechanical stability of anterior cervical pedicle screw implantation for osteoporosis in the vertebra. METHODS:A total of 12 human cervical vertebrae were col ected, including 6 vertebrae with normal bone density and 6 vertebrae with osteoporosis. Data of 60 vertebra specimens were analyzed. 30 osteoporotic specimens implanted in anterior cervical pedicle screw were set as anterior cervical pedicle screw group. 30 normal specimens implanted in anterior cervical pedicle screw were set as anterior vertebral screw group. According to bone density, 40 vertebrae were col ected in above two groups, and were considered as normal bone mineral density group, instant osteoporosis group, fatigue normal bone mineral density group, and fatigue osteoporosis group (n=10). Bone mineral density of each vertebra was detected using dual-energy X-ray absorptiometry. Biomechanical index of two kinds of screws was detected using ElectroForce 3510 material testing machine. RESULTS AND CONCLUSION:Bone mineral content, vertebral screw pul-out strength, vertebral screw pul-out stiffness, pedicle screw pul-out strength, and pedicle screw pul-out stiffness were significantly higher in the anterior cervical pedicle screw group than in the anterior vertebral screw group (P<0.05). The maximum axial pul-out strength of normal bone mineral density group, instant osteoporosis group, fatigue normal bone mineral density group, and fatigue osteoporosis group was significantly higher than in the anterior vertebral screw group (P<0.05). The results confirmed that osteoporotic vertebral biomechanical performance is more stable in anterior pedicle screws compared with the anterior vertebral screw.