ObjectiveTo determine the diurnal variation,moon phase,and seasonal variation in the onset of stroke. MethodsThe time parameters,including the time,the moon phase and the season of the stroke occurrence for 905 in-patients from 1989-2001 were investigated.ResultsThere was a significant difference (D=0.155,P<0.01) for circadian rhythm in the onset of 321 cases with cerebral infarction,and the incidence frequency at dawn was much higher than that at other time,but the difference of the moon phase and seasonal rhythm in the onset of cerebral infarction were not significant(P>0.05).There were not diurnal variation,moon phase and seasonal rhythms in the onset for cerebral haemorrhage(P>0.05).ConclusionsThe cerebral infarction occur more frequently at dawn time.

Tumor marker is a hot subject of research in oncology field,which would promote the diagnosis and treatment in clinic.Tumor markers of children's germ cell tumor include α-fetoprotein,glypican 3,SALL4,glial cell line-derived neurotropic factor receptorα-1(GFRα-1),Ki-67,octamer-binding transcription factor4,CD117,D2-40 and CD30.SALL4 would be used as a new tumor marker for germ cell tumor,specially for yolk sac tumor.GFRα-1 can be helpful for the diagnosis of immature teratoma.

OBJECTIVE:To observe the clinical efficacy and safety of cinepazide maleate combined with oxiracetam in the treat-ment of vascular dementia. METHODS:62 patients with vascular dementia were randomly divided into treatment group and control group,with 31 patients in each group. Patients in control group were treated with Oxiracetam injection 4g/d for 3weeks. Patients in treat-ment group were treated with cinepazide maleate 160 mg/d for 3 weeks on the basis of control group. The patients of 2 groups were exam-ined with Mini-mental State Examination (MMSE)and Activity of Daily Living Scale (ADL)before and after treatment. RESULTS:There was statistically significant difference in MMSE and ADL scores of 2 groups before and after treatment(P<0.05);MMSE score and ADL score of treatment group was better than that of control group,with statistical significance(P<0.05);Obvious ADR was not found in both groups. CONCLUSIONS:Cinepazide maleate combined with oxiracetam can significantly improve ADL and MMSE score in patients with vascular dementia,improve the quality of life,showing significant and good clinical efficacy.

ObjectiveTo study the effect of Xiangdan point injection in upper limb spasticity after stroke. Methods56 cases were divided into two groups: 28 cases for treatment group were treated with point injection Xiangdan, combined with routine therapy. Another 28 cases for control were only routinely therapy. ResultsThe cure rate of the treatment group was 29% ,the effective rates was 100%, while that of control group was 11% and 25% respectively (P<0.01).Conclusions Xiangdan point injection is more effective in upper limb spasticity after stroke than only routine therapy.

112 cases with hemiplegia were randomly divided into three groups,training,acupuncture,and integrated training and acupuncture.Their effects were observed for three monthes.Results:the effect of the integrated training and acupuncture group was significantly better than the other two groups(P＜0.01).The results comfirmed that the motor function of the hemiplegic limbs was enhanced effectively with the integrated training and acupuncture.

Objective To investigate the genetic characteristics of viruses associated with viral diarrhea in Jilin Province, in order to provide a scientific basis for making more effective prevention and control strategies of viral diarrhea.Methods A total of 211 fecal specimens from hospitalized children under 5 years of age with diarrhea and 7 sewage samples were collected in Jilin Province from January to July 2023. Initial screening was performed using qPCR, and positive specimens were further analyzed by RT-PCR to amplify target genes. Sequencing was conducted, and phylogenetic trees were constructed to analyze genetic relationships.Results In fecal specimens, the positive rates for rotavirus(RV), Norovirus(NoV),Sapovirus(SaV), astrovirus(AstV), and adenovirus(AdV) were 10. 9%, 48. 3%, 4. 3%, 1. 4%, and 5. 7%, respectively. In sewage samples, 5 AstV and 4 NoV were detected, along with 3 samples each of RV, SaV, and AdV. Sequencing and genotyping results revealed that RV G8P [8] was the predominant circulating strain. Among NoV strains, GⅡ.4 [P16] was the most prevalent, followed by GⅡ.4 [P31]. In fecal samples, GⅠ group strains included GⅠ.3 [P13] and GⅠ.1 [P1]. One SaV strain of GⅡ.5 was identified. For AstV, three strains of HAstV-1a and one strain of HAstV-5 were detected. Among AdV strains, three were identified as HAdV-41, three as HAdV-2, one as HAdV-1, and one as HAdV-5.Conclusion The pathogenic genes of viral diarrhea in Jilin Province are diverse, the dominant strains of RV have changed, and domestic sewage contains a variety of diarrhea viruses.

OBJECTIVE:To develop the data processing program of Digoxin blood drug concentration(DBDP)and to determine the range of effective blood concentration of Digoxin.METHODS:The DBDP program was developed by VISUAL BASIC6.0and run in Windows95system.The data of blood drug concentration,which was determined with flurescence polarization immunoassay(FPIA)in our hospital,was analyzed with DBDP program and effective blood drug concentration range of Digoxin was set up.RESULTS:The DBDP program integrated functions including pharmacokinetic parameter estimation,establishment of the effective blood drug concentration range,management of the data of the blood drug concentration moni?toring,automatic addition of patient's file,multifunctional fuzzy searching,retrospective analysis and clinical query about drug use.The effective blood drug concentration range of Digoxin was0.58～2.01ng/ml.CONCLUSION:The DBDP program is a very practical tool for the development of therapeutic drug monitoring and it is very necessary to develop blood drug concen?tration monitoring and establish the effective blood drug concentration range.

Objective To observe the effect of atmospheric pollution on workers'α1-antitrypsin concentrationb in petrochemical enterprises.Methods During health examination,the workers were indivded into 3 groups according tO the length of job experience.Ⅰ group(<10 years)included 291 cases,aged 22-35 years old,Ⅱ group(10-20 years)included 634 cases,aged 33-45 years old,and Ⅲgroup(>20 years)included 427 cases,aged 44-55 years old.The normal control were individed int different group according to the status of petrochemical workers.,all the serum samples were detected on automatic biochemical analyzer,α1-antitrypsin levels were analyzed between the different groups.Results In group Ⅰ,the α1-AT concentration was 2.58±0.38 g/L(P>0.05)without significant difference compared with control.In group Ⅱ and Ⅲ,the concentration α1-AT were 2.02±0.43 g/L and 1.72±0.41 g/L respectively with significant differences compared with control.And the significant difference existed among the three groups of petrochemical workers..But the sigficant difference did not exist between different group of control people.Conclusion During the process of oil refining,the plants exhausted the polluted gas of nitrogen dioxide,olefins,alkanes,alcohols,carbonyl and other harmful substances,it influenced theα-AT levels of body.It is a good suggestion to decreas the petrochemical pollution,and to monitor the serum concentration of α-AT content among petrochemical workers,it might to reduce the incidence of lung disease of great significance.

Objective To investigate the disease categories of the upper alimentary tract and the ratio of Hp infection in countryside children. Methods 96 children with complaint in upper digestive tracts underwent gastro-scope and in each patient,a piece of mueosal tissue in sinus ventrieuli was taken out to perform Hp urase test. Results All of the 96 children were detected abnormal including chronic superficial gastritis (n = 63), ulcer of duodenal bulb(n = 13) ,enteritis of duodenal bulb(n =7) ,gastritis with bile reflux(n =4) ,erosive gastritis(n =2) ,ascariasis (n = 6), aneylestomiasis(n = 3). The positive rate was 100%. 49/96 (51%) were positive in Hp test, including 36/ 63(57.1%) of chronic gastritis ,10/13(76.9%) of ulcer of duodenal bulb,2/7 (28.6%) of enteritis of duodenum. Hp were positive in 11 (35.5%) in the group with age range from 3 to 6 years old and positive in 38 (58.5%) in the group with age range from 7 to 12 years old. Conclusion Chronic superficial gastritis and ulcer of duodenal bulb were the main findings in gastrescopy in children with a upper digestive tract complaint. Hp infection ratio was highest in patients with ulcer of duodenal bulb in our study, and followed by the chronic gastritis. And the infection rate in-creased with the age increased.

BACKGROUND:Studies have shown that Ganoderma lucidum polysaccharides have biological activities of anti-viral, anti-tumor, enhance immunity, anti-oxidative and anti-aging. OBJECTIVE:To observe the biological effect of Ganoderma lucidum polysaccharides on the antioxidant capacity of D-galactose induced aging mice skin tissues, and to observe the effect in postponing skin aging. METHODS:Forty-four 2-month-old Kunming mice were randomly divided into four groups:normal control group, aging model group, vitamin E group, and Ganoderma lucidum polysaccharide group. Rats in the last three groups received subcutaneous injection (nape area) of Ganoderma lucidum polysaccharide to establish rat aging models, and intragastric administration of corresponding drugs or saline was performed. After 42 days, pathological sections of back skin were obtained to assess the morphological changes of skin tissues, measure the epidermal and dermal thickness, and detect the superoxide dismutase levels and the expression of CuZn-superoxide dismutase mRNA. RESULTS AND CONCLUSION:The epidermal and dermal thickness in the vitamin E group and Ganoderma lucidum polysaccharide group were increased when compared with the aging model group. The superoxide dismutase levels in the Ganoderma lucidum polysaccharide group were significantly higher than those in the other two groups. The decreased degree of cycle threshold value in the Ganoderma lucidum polysaccharide group was lower than that in the other two groups. The results indicate that Ganoderma lucidum polysaccharide can increase the epidermal and dermal thickness, improve the skin tissue structure, and enhance the superoxide dismutase levels and the expression of CuZn-superoxide dismutase mRNA.