The deficiency in islet ?-cell secretion and (or) the decrease in insulin sensitivity of target tissue are the important pathophysiological mechanisms of diabetes. The methods for the assessment and evaluation of ?-cell function can be divided into: pulsatile insulin secretion, glucose secretagogues, non-glucose secretagogues (phase, peak value and duration) and other secretions. While the suitable method was chosen for the evaluation of ?-cell function, the sensitivity and specificity of different methods in the natural history of diabetes and the objective of evaluation should be all taken into account.

Objective:To analyze influence of vaginal hysteroscopy and laparoscopic surgery on tubal pregnancy patients’ surgical outcome and inflammatory stress response.Methods: Chosen 86 cases of tubal pregnancy patients between July 2012 to July 2013 in our hospital undergoing surgery as study object, according to a random number table divided into hysteroscopy group (43 cases) and laparoscopic group (43 cases), compared surgical outcomes, perioperative differences in the level of inflammation and stress level.Results: No significant difference between hysteroscopy group and laparoscopic group on surgical success rate and ipsilateral tubal patency statistically(x2=0.241,x2=0.193;P>0.05), postoperative intrauterine pregnancy rate was higher than laparoscopic group, again ectopic pregnancy rate was less than laparoscopic patients(x2=7.294,x2=8.283;P<0.05); hysteroscopic surgery group E, Cor, IL-2, IL-6, IL-8, CRP levels were lower than laparoscopic group(t=6.842,t=6.283,t=8.536,t=6.106,t=6.283,t=6.376;P<0.05),TSH levels was higher than laparoscopic group(t=6.823,P<0.05).Conclusion:Vaginal hysteroscopy and laparoscopy can be used for the treatment of tubal pregnancy, hysteroscopy has an advantage in improving patient again intrauterine pregnancy, reduce postoperative systemic stress and inflammation levels, and promote the recovery of patients after surgery respect.

Objective To research the position transition training effect of general practitioner of northeast district of Chongqing,and find out the problems and their reasons.Methods 326 students and 150 unit leaders were chosen to make a questionnaire survey(recycled 307,150).The Kirkpatrick model was also used for data analysis from 4 levels.The result was described in percentage.Results Reaction Level:42.02％(129/307) of the trainees were not satisfied with the time arrangement of clinical medicine training; Study Level:3 courses were at high failure rate 19.54％(60/307),22.8％(70/307),17.92％(55/307); Behavior Level:81.43％(250/307)of the trainees considered the training effective; Result Level:94.00％(141/150)unit leaders considered the training had a positive impact.Conclusion The time arrangement of clinical medicine training is mostly unsatisfied and the ability of instrument using and result interpretation is weak.Most people consider the position transition training is effective.

Objective: To analyze the effect of single-hole thoracoscopic surgical treatment on elderly spontaneous pneumothorax patients’ postoperative assessment and serological markers and explore single-hole thoracoscopic surgery application. Methods:78 cases of elderly patients with spontaneous pneumothorax were retrospectively analyzed, and divided into single-hole thoracoscopy group with 35 patients and conventional thoracoscopic group with 43 patients. Then the surgery indicators, postoperative pain scores and perioperative serum target level differences were compared between the two groups. Results: The operative time, postoperative drainage time and total hospital stays of single-hole thoracoscopic surgery patients were shorter than that of conventional thoracoscopic group. The differences were statistically significant (t=7.183, t=5.294, t=6.938;P<0.05). The blood loss and postoperative drainage were less. The differences were statistically significant (t=6.883, t=8.735; P<0.05). The postoperative pain scores within one week was less than conventional thoracoscopic group. The differences were also statistically significant (t=6.882, t=5.724, t=5.284;P<0.05). The serological indicators, CRP, IL-1, TNF-α,α1-AT levels of patients in the single-hole thoracoscopy group were lower than the conventional group (t=8.293, t=7.274, t=9.284, t=7.183;P<0.05). Conclusion:VATS and conventional hole thoracoscopic surgery can be used for treatment of spontaneous pneumothorax in elderly, but single-hole thoracoscopy has more advantages in reducing surgical trauma and postoperative pain and optimize systemic inflammation associated factors.

Objective Drug resistance is the main reason for chemotherapy failure ,it is to be solved how to overcome this . Recent studies indicate that epithelial-mesenchymaltransition (EM T ) may involved in chemotherapy resistance for some types of cancers .But it is still unknown whether EM T is involved in chemotherapy resistance for colorectal carcinoma .This study was con-ducted to investigate the relation between EM T and colorectal cancer chemotherapy resistance .Methods Abilities of migration and invasion of Oxaliplatin resistant colorectal carcinoma cell lines LOVO /L-OHP and wild type colorectal carcinoma cell lines LOVO were investigated by transwell migration and transwell invasion assays .The level of E-cadherin and Vimentin was detected by West-ern blot .EM T of LOVO was induced by GSK-3β inhibitor SB415286 treatment and validated by transwell migration and transwell invasion assays and Western blot for E-cadherin and Vimentin .The sensitivity of LOVO to Oxaliplatin after SB 415286 inducing EM T was evaluated by M TT and Annexin-V /PI assays .Results EM T phenotype was confirmed by morphology feature ,results of transwell migration and invasion assays and level changes of E-cadherin and Vimentin .In addition ,the results of proliferation and apoptosis tests showed that the sensitivity of LOVO to Oxaliplatin after SB 415286 inducing EM T decreased significantly ,which means chemotherapy resistance .Conclusion The direct association between EM T and chemotherapy resistance for colorectal carci -noma is proved in this study ,which provides basis for intervening chemotherapy resistance and new researching strategies .

Humans ; Olfaction Disorders ; genetics ; Smell ; genetics

Genetic Predisposition to Disease ; Humans ; Olfaction Disorders ; congenital ; genetics

Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Middle Aged ; Myasthenia Gravis ; drug therapy ; therapy ; Young Adult

Objective To evaluate the diagnosis value of C-12 multiple tumor marker protein chip detective system for lung cancer. Methods The serum levels of 12 tumor makers were measured in 172 lung cancer patiens,52 pulmonary benign diseases patients. All lung cancer patients were definitly diagnosised by cytology or histology, including 89 squamous cell carcinoma patients, 72 bronchogenic adenocarcinoma patients, 11 small cell lung cancer patients; 12 patients in stage I, 28 patients in stageII, 65 patients in stage III, 67 patients in stage IV. The 12 common tumor markers in serum included CA199, NSE, CEA, CA242, CA125, CA153, AFP, Ferrtin, free-PSA, PSA, HGH, ?-HCG. Results At least one kind of tumor maker was found higher in 128 of the 172 lung cancer patients, the positive rate was 74.42%, and in 5 of the 52 pulmonary benign diseases patients, the positive rate was 9.62%,it is statistical significance between two the groups (P0.05) in different pathological types lung cancer patients .The positive rate was statistical significance in different stage lung cancer patients , the highest positive rate which in stage IV patients was 79.2% (P

Objective To check serum protein of children′s Hirschsprung′s disease(HD) and sift specific protein marker which was used in constructing of HD screening and early diagnosis of serum protein fingerprint model.Methods Surface-enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS) was applied to detect protein mass spectrometry of serum specimens in 82 cases(HD group 42 cases,20 cases of other types of obstruction,healthy control group 20 cases) and data were analyzed by bioinformatics methods(support vector machine).Results 1.For HD group and healthy control group:selected 3 M/Z in 3 221.7,5 639.2,6 884.2 protein markers were selected,HD early screening and diagnostic model was established,3 markers in HD low expression,the expressions of them in HD group and healthy control group were 378.29?273.34,295.65?159.38,444.13?254.06 and 1 428.18?1 192.61,1 039.60?785.64,1 115.72?680.48,respectively.There were significant differences in two groups(Pa0.05).Conclusions The establishment of serum protein fingerprint model of HD by SELDI-TOF-MS support vector machine could screen and diagnose HD early,which is a new method of better specificity,high sensitivity and is worthy of further research and application.