1.Assessment of islet ?-cell function: from basic research to clinical practice
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
The deficiency in islet ?-cell secretion and (or) the decrease in insulin sensitivity of target tissue are the important pathophysiological mechanisms of diabetes. The methods for the assessment and evaluation of ?-cell function can be divided into: pulsatile insulin secretion, glucose secretagogues, non-glucose secretagogues (phase, peak value and duration) and other secretions. While the suitable method was chosen for the evaluation of ?-cell function, the sensitivity and specificity of different methods in the natural history of diabetes and the objective of evaluation should be all taken into account.
2.A study on the position transition training effect of general practitioner of Northeast District of Chongqing
Chinese Journal of Medical Education Research 2014;13(12):1242-1245
Objective To research the position transition training effect of general practitioner of northeast district of Chongqing,and find out the problems and their reasons.Methods 326 students and 150 unit leaders were chosen to make a questionnaire survey(recycled 307,150).The Kirkpatrick model was also used for data analysis from 4 levels.The result was described in percentage.Results Reaction Level:42.02%(129/307) of the trainees were not satisfied with the time arrangement of clinical medicine training; Study Level:3 courses were at high failure rate 19.54%(60/307),22.8%(70/307),17.92%(55/307); Behavior Level:81.43%(250/307)of the trainees considered the training effective; Result Level:94.00%(141/150)unit leaders considered the training had a positive impact.Conclusion The time arrangement of clinical medicine training is mostly unsatisfied and the ability of instrument using and result interpretation is weak.Most people consider the position transition training is effective.
3.The study on association between epithelial mesenchymal transition and Oxaliplatin resistance for colorectal carcinoma
Chongqing Medicine 2016;45(17):2308-2311
Objective Drug resistance is the main reason for chemotherapy failure ,it is to be solved how to overcome this . Recent studies indicate that epithelial-mesenchymaltransition (EM T ) may involved in chemotherapy resistance for some types of cancers .But it is still unknown whether EM T is involved in chemotherapy resistance for colorectal carcinoma .This study was con-ducted to investigate the relation between EM T and colorectal cancer chemotherapy resistance .Methods Abilities of migration and invasion of Oxaliplatin resistant colorectal carcinoma cell lines LOVO /L-OHP and wild type colorectal carcinoma cell lines LOVO were investigated by transwell migration and transwell invasion assays .The level of E-cadherin and Vimentin was detected by West-ern blot .EM T of LOVO was induced by GSK-3β inhibitor SB415286 treatment and validated by transwell migration and transwell invasion assays and Western blot for E-cadherin and Vimentin .The sensitivity of LOVO to Oxaliplatin after SB 415286 inducing EM T was evaluated by M TT and Annexin-V /PI assays .Results EM T phenotype was confirmed by morphology feature ,results of transwell migration and invasion assays and level changes of E-cadherin and Vimentin .In addition ,the results of proliferation and apoptosis tests showed that the sensitivity of LOVO to Oxaliplatin after SB 415286 inducing EM T decreased significantly ,which means chemotherapy resistance .Conclusion The direct association between EM T and chemotherapy resistance for colorectal carci -noma is proved in this study ,which provides basis for intervening chemotherapy resistance and new researching strategies .
4.Influence of vaginal hysteroscopy and laparoscopic surgery on tubal pregnancy patients’ surgical outcome and inflammatory stress response
China Medical Equipment 2016;13(4):85-87,88
Objective:To analyze influence of vaginal hysteroscopy and laparoscopic surgery on tubal pregnancy patients’ surgical outcome and inflammatory stress response.Methods: Chosen 86 cases of tubal pregnancy patients between July 2012 to July 2013 in our hospital undergoing surgery as study object, according to a random number table divided into hysteroscopy group (43 cases) and laparoscopic group (43 cases), compared surgical outcomes, perioperative differences in the level of inflammation and stress level.Results: No significant difference between hysteroscopy group and laparoscopic group on surgical success rate and ipsilateral tubal patency statistically(x2=0.241,x2=0.193;P>0.05), postoperative intrauterine pregnancy rate was higher than laparoscopic group, again ectopic pregnancy rate was less than laparoscopic patients(x2=7.294,x2=8.283;P<0.05); hysteroscopic surgery group E, Cor, IL-2, IL-6, IL-8, CRP levels were lower than laparoscopic group(t=6.842,t=6.283,t=8.536,t=6.106,t=6.283,t=6.376;P<0.05),TSH levels was higher than laparoscopic group(t=6.823,P<0.05).Conclusion:Vaginal hysteroscopy and laparoscopy can be used for the treatment of tubal pregnancy, hysteroscopy has an advantage in improving patient again intrauterine pregnancy, reduce postoperative systemic stress and inflammation levels, and promote the recovery of patients after surgery respect.
5.Effect of single-hole thoracoscopic surgical treatment on elderly spontaneous ;pneumothorax patients’ postoperative assessment and serological markers
China Medical Equipment 2016;13(7):84-86,87
Objective: To analyze the effect of single-hole thoracoscopic surgical treatment on elderly spontaneous pneumothorax patients’ postoperative assessment and serological markers and explore single-hole thoracoscopic surgery application. Methods:78 cases of elderly patients with spontaneous pneumothorax were retrospectively analyzed, and divided into single-hole thoracoscopy group with 35 patients and conventional thoracoscopic group with 43 patients. Then the surgery indicators, postoperative pain scores and perioperative serum target level differences were compared between the two groups. Results: The operative time, postoperative drainage time and total hospital stays of single-hole thoracoscopic surgery patients were shorter than that of conventional thoracoscopic group. The differences were statistically significant (t=7.183, t=5.294, t=6.938;P<0.05). The blood loss and postoperative drainage were less. The differences were statistically significant (t=6.883, t=8.735; P<0.05). The postoperative pain scores within one week was less than conventional thoracoscopic group. The differences were also statistically significant (t=6.882, t=5.724, t=5.284;P<0.05). The serological indicators, CRP, IL-1, TNF-α,α1-AT levels of patients in the single-hole thoracoscopy group were lower than the conventional group (t=8.293, t=7.274, t=9.284, t=7.183;P<0.05). Conclusion:VATS and conventional hole thoracoscopic surgery can be used for treatment of spontaneous pneumothorax in elderly, but single-hole thoracoscopy has more advantages in reducing surgical trauma and postoperative pain and optimize systemic inflammation associated factors.
8.Operation of new rural cooperative medicine in Miluo,Hunan Province——A case study
Menglong XIANG ; Ying LI ; Jia CAO
Chinese Journal of Disease Control & Prevention 2009;0(01):-
Objective To understand the implementation of New Rural Cooperative Medicine(NRCM)in Hunan Province carried out in Miluo City.Methods Qualitative study was combined with secondary data collection.Results Government in Miluo took initatives to organize NRCM and financed it with the NRCM Funds;each peasant could enjoy NRCM by paying only 10 yuan,and the NRCM participation rate reached 73.6%;currently reimbursement mainly focused on hospitalization,lowest-payment for seeking care,and reimbursement proportion varied in health facilities at different levels;the health facilities and peasants benefited a lot from NRCM.Conclusions The NRCM participation is increasing,funds allocated properly,peasants welcoming it.Further measures for NRCM improvement may include allocating more funds,perfecting reimbursement procedures.Besides,the NRCM for some population like persons without children or suffering severe disease awaits solution.
9.The impact of polymorphism of calpain-10 gene on glucose intolerance status, insulin secretion and insulin sensitivity
Kunsan XIANG ; Taishan ZHENG ; Weiping JIA
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Objective To study the impact of single nucleotide polymorphism 43 (UCSNP43) of calpain 10 gene (CAPN 10) at NIDDM1 locus, chromosome 2q37.3 on glucose intolerance status and its intermediate traits. Methods The studied population consisted of 320 Chinese subjects in Shanghai 〔normal glucose tolerance (NGT) 148, impaired glucose tolerance (IGT) 44 and type 2 diabetes (T2DM) 128〕. Plasma glucose, insulin, C peptide and free fatty acid levels were measured during fasting and 30, 60, 120 and 180 min after oral 75 g glucose load. The tissue insulin sensitivity and islet beta cell insulin secretion were assessed by HOMA formulae and the ratio of the increment of insulin and glucose levels at 30 min. The CAPN 10 UCSNP43 was genotyped by DNA direct sequencing. Results (1) The frequencies of GG, GA and AA genotypes of CAPN 10 UCSNP 43 were 0.80, 0.18 and 0.02 respectively. The G allele frequency was 0.89, which was statistically significant higher than those in Caucasians, Mexican Americans and Pima Indians (all P
10.Study on the relation between insulin resistance and total and regional body fat in overweight and obese individuals
Lei CHEN ; Weiping JIA ; Kunsan XIANG
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Objective To use the extended hyperinsulinemic euglycemic clamp technique for the study of insulin sensitivity in normal weight and normal glucose tolerant obese Chinese, and also, for the study of insulin sensitivity in relation to body adipose depots and distribution. Methods Twenty two Chinese 〔9 with normal weight (BMI