1.Influence of Marital Status on the Quality of Life of Chinese Adult Patients with Epilepsy
Wang FU-LI ; Gu XIANG-MIN ; Hao BAO-YUN ; Wang SHAN ; Chen ZE-JIE ; Ding CHENG-YUN
Chinese Medical Journal 2017;130(1):83-87
Background:Epilepsy is a chronic disorder characterized by recurrent seizures and has significant psychological and social consequence for everyday living.Epilepsy affects various aspects of ones' social life.The present study aimed to investigate the influence of marital status on the quality of life of adult Chinese patients with epilepsy.Methods:This study surveyed 805 Chinese adults who have been clinically diagnosed with epilepsy for longer than 1 year in 11 hospitals in Beijing.In this survey,532 (66.1%) participants were married.All of them completed the case report form with enquiries on demographic data,social factors,and illness.The marriage status of adult epileptic quality of life was the dependent variable,and demographic data and clinical data were independent variables,analyzed through the multiple linear regression analysis methods.The patients' quality of life was assessed using the Quality of Life in patients with Epilepsy-31 items (QOLIE-31) questionnaire,the Patient Health Questionnaire-9 items (PHQ-9),and the Generalized Anxiety Disorder-7 items (GAD-7).Results:The PHQ-9 and GAD-7 scores in the unmarried group (PHQ-9 =6.0 and GAD-7 =5.0) were significantly higher than that of the married group (PHQ-9 =4.0 and GAD-7 =3.0).The scores of married adult patients with epilepsy on QOLIE (61.8 ± 15.3) and social function (70.9 ± 22.7) were higher than the scores of the unmarried patients aged between 20 and 44 years.The scores of married adult epileptics on the QOLIE (58.4 ± 14.6) and the energy/fatigue (62.1 ± 20.4) were higher than the scores of the unmarried patients (QOLIE =58.4 ± 14.6 and the energy/fatigue =62.1 ± 20.4) aged between 45 and 59 years.For the adult epilepsy patients,depression,anxiety,seizures within the last year,disease course,medical expense category,and marriage* age are negatively correlated with the quality of life.Occupation,educational level,and average monthly income are closely related to the quality of life.Conclusions:Married adult epileptic patients have better quality of life than that of unmarried adult patients in young and middle-aged age groups.Unmarried adult patients with epilepsy are more anxious and depressed than married adult patients.
2.Comparative study of left bundle branch pacing in cardiac synchronization treatment system
Chinese Journal of Geriatric Heart Brain and Vessel Diseases 2024;26(6):637-641
Objective To evaluate the clinical value of left bundle branch pacing(LBBP)in HF pa-tients requiring cardiac resynchronization therapy(CRT).Methods A total of 128 HF patients who met the indicatons for CRT Class Ⅰ and Ⅱ and admitted in Department of Cardiology of Northern Jiangsu People's Hospital from January 2018 to January 2023 were consecutively recrui-ted,and 32 patients of them having left bundle branch electrode implantation due to failure of cor-onary sinus electrode implantation were assigned into the LBBP group,and the rest 96 patients getting conventional biventricular pacing(BVP)into the BVP group.The pacing parameters and other indicators were observed in the two groups at the time of implantation.Follow-up visits were made to the Special Clinic of"Arrhythmia and Cardiac Pacemaker"in Northern Jiangsu People's Hospital at 1,3,6,and 12 months after surgery,and then,the visits were conducted every 3 to 4 months thereafter.Clinical evaluation,12-lead electrocardiography,echocardiography,detections for programmable controller and pacemaker function and other tests were completed at each follow-up.Readmission or death events due to HF were recorded.Results Compared with the preoperative results,both groups obtained shorter QRS duration,better NYHA class,smaller LVEDD and increased LVEF at the last follow-up(P<0.05).At the time point,shorter QRS du-ration,better NYHA class and higher LVEF were observed in the LBBP group than the BVP group(P<0.05).There were no significant differences in pacing parameters between the two groups at the time of implantation and the last follow-up(P>0.05).No statistical difference was found in readmission rate between the LBBP group and the BVP group(18.75%vs 16.67%,P>0.05).Conclusion LBBP is safe and effective,and can improve the cardiac function of HF pa-tients.LBBP may provide a new option for patients who have failed traditional CRT.
3.Dysbiosis of lung commensal bacteria in the process of lung epithelial-mesenchymal transition in mice with silicosis
China Occupational Medicine 2022;49(05):514-
Objective -
To investigate the effect of lung flora dysbiosis on the process of pulmonary fibrosis and lung epithelial
( ) Methods -
mesenchymal transition EMT in mice with silicosis. Male C57BL/6 mice of specific pathogen free grade were
, , , ( )
randomly divided into the blank control group silicosis model group solvent control group vancomycin VM + ampicillin
( ) , ( ) ( ) ,
AMP group metronidazole MNZ + neomycin NEO group and mixed treatment group 12 mice in each group. Except for
, ,
the blank control group which was given 20.0 µL of 0.9% NaCl solution the other five groups of mice were dosed with 20.0 µL
of silica dust suspension at a mass concentration of 250.0 g/L using a single tracheal drip to establish the silicosis mouse model.
:
The intranasal drip method was used to treat silicosis mice in each group as following mice in the solvent control group were
- ; ;
given double distilled water mice in the VM+AMP group were given VM at a mass concentration of 0.5 g/L and AMP at 1.0 g/L
;
mice in the MNZ+NEO group were given MNZ at a mass concentration of 1.0 g/L and NEO at 1.0 g/L mice in the mixed
,
treatment group were given the same doses of the four antibiotics mentioned above all in a drip volume of 50.0 µL. Silicosis
, ,
mice were treated seven days and half an hour before silica dusting and 7 14 and 21 days after silica dusting. Mouse lungtissue was collected aseptically 28 days after silica dusting. Hematoxylin eosin and Masson trichrome staining methods were
-
used to observe the pathological changes. Western blotting was used to detect the relative protein expression of α smooth muscle
( - ), - ( - ) ( )
actin α SMA E cadherin E CAD and vimentin VIM . Immunohistochemistry was used to detect the relative expression of
- -
E CAD and VIM. Real time fluorescence quantitative polymerase chain reaction was used to detect the expression levels of
(Col1a2) Results
collagen type Ⅰ alpha 2 mRNA in lung tissues. The histopathological results showed that the alveoli of the
,
blank control group were thin and structurally intact with few surrounding infiltrating inflammatory cells and no abnormal
,
distribution of collagen fibers. The alveoli of the silicosis model group were structurally disorganized with a large number of
, ,
infiltrating inflammatory cells thickened alveolar walls and cellular fibrous nodules with abundant blue collagen deposit. In the
, ,
VM+AMP group MNZ+NEO group and the mixed treatment group the inflammation and fibrosis were reduced with diferent
degrees in the lung tissues compared to the silicosis model group and the solvent control group. The relative expression levels of
- , Col1a2
α SMA VIM protein and mRNA in lung tissues of mice in the silicosis model group were higher than those in the blank
( P ), -CAD
control group all <0.05 and the relative expression levels of E protein were lower than those in the blank control
(P ) - , Col1a2
group <0.05 . The relative expression levels of α SMA VIM protein and mRNA in lung tissues of mice in the MNZ+
( P ), -CAD
NEO group and the mixed treatment group were lower all <0.05 and the relative expression levels of E protein were
(P ), Conclusion
higher <0.05 when compared with the silicosis model group and the solvent control group. Pulmonary fibrosis
, -
was reduced in silicosis mice with interventions in lung flora where anaerobic and gram negative bacteria affected pulmonary
fibrosis and dysbiosis of the lung flora affected pulmonary EMT.
4.Clinicopathological and related gene analysis in gastric adenocarcinoma and their correlation with prognosis
Xing-Hao NI ; Chuan-Ding YU ; Shen-Hua XU ; Xing-Ming ZHOU ; Yu-Tiang LIN ; Gu ZHANG ; Chi-Hong ZHU ; Xiang-Lin LIU ;
Cancer Research and Clinic 2006;0(10):-
Scm in volume(42/60),multiple site involvement(44/60),blood type"O"(31/41),in comparison with those of survival group,and the difference was statistically significant.C-erbB-2,p16,p53,P-gp,CD_(44) and CD_(25)expression were not significantly different in these two groups. Conclusion The clinical stage, lymph node metastasis,lymphatic tumor emboli and/or neural involvement,infiltration depth,histological dif- ferentiation,tumor volume,involvement extension are important prognostic factors in patients with gastric can- cer,while the significance of cancer-related gene expression in gastric carcinomas needs to be studied further.
5. Ovarian clear cell borderline tumour: a clinicopathologic analysis
Weiyong GU ; Lihong ZHANG ; Hao ZHANG ; Qiuzhi PAN ; Yuqing QU ; Xiang TAO ; Yan NING
Chinese Journal of Pathology 2018;47(8):622-626
Objective:
To investigate the clinical and pathological characteristics and prognosis of ovarian clear cell borderline tumor.
Methods:
A total of 12 cases of ovarian clear cell borderline tumors recorded were collected from May 2011 to December 2017 at Obstetrics and Gynecology Hospital, Fudan University.Clinical histories were retrieved and pathological slides were reviewed.
Results:
The age of the patients ranged from 35 to 65 years with a mean age of 52 years. Seven cases were associated with cystic endometriosis of the ovary. All tumors consisted of irregular and crowded glands or cysts embedded in a fibromatous stroma. The cysts and glands were lined by mild to moderate atypical cells.CK7 and HNF-1β were expressed in all cases, and Naspin A was expressed in 11 cases. ARID1A expression was absent in 5 cases and p53 showed wild-type expression. None of the cases developed recurrence during follow-up ranging from 7 to 79 months.
Conclusions
Ovarian clear cell borderline tumor may be associated with endometriosis and tumor suppressor gene ARIDA. The tumor has a good prognosis without recurrence and progression to carcinoma.
6.Effects of occupational mercury exposure on the immune function of workers
China Occupational Medicine 2022;49(06):657-661
Objective
To investigate the effects of mercury on T lymphocytes and serum immune indexes of workers with
Methods
occupational mercury exposure. A total of 45 workers with occupational mercury exposure were selected as the
,
mercury exposure group and 47 workers without occupational mercury exposure were selected as the control group using the
judgment sampling method. Cold atomic absorption spectrometry was used to detect the urinary mercury level of the two groups.
( ) +, + +, + + - +
Flow cytometry was used to detect the proportion of cluster of differentiation CD 3 CD3CD4 CD3CD8 and CD3CD19
, - ( - ) - ( - )
cells in peripheral blood and the levels of tumor necrosis factor α TNF α and interleukin 8 IL 8 in serum. The levels of
( ) , Results
immunoglobulin Ig A IgG and IgM in serum were measured by immune nephelometry. The urinary mercury level of
( : vs ,P )
individuals in the mercury exposed group was higher than that of the control group median 92.7 13.2 μg/g Cr <0.01 . The
+, + +, - +
proportion of CD3 CD3CD4 CD3CD19 cells in peripheral blood and serum IgG level in the mercury exposed group
( P ), - - ( P )
decreased all <0.05 and the serum TNF α and IL 8 levels increased all <0.01 compared with the control group. Urinary
-
+
mercury level was negatively correlated with the proportion of CD3CD19 cells in peripheral blood and serum IgG level in the
[ (r) , , P ],
study subjects Spearman correlation coefficient S were −0.21 and −0.31 respectively all <0.05 and positively
- - (r , , P ) ,
correlated with serum TNF α and IL 8 levels S were 0.36 and 0.39 respectively all <0.05 . However the urinary mercury
( P ), +, + +,
level was neither correlated with IgA and IgM levels in serum all >0.05 nor with the proportion of CD3 CD3CD4
+ + ( P ) Conclusion
CD3CD8 cells in peripheral blood all >0.05 . Occupational exposure to mercury can lead to abnormal
,
changes in peripheral blood T lymphocyte subsets B lymphocytes and serum immune factors in workers. The mercury load of occupational mercury exposure workers may impact their immune function.
7.Genetics and clinical study of Chinese kindreds with dentatorubral pallidoluysian atrophy.
Xin ZHANG ; Ying HAO ; Wei-hong GU ; Yuan-yuan CHEN ; Jin ZHANG ; Guo-xiang WANG ; Kang WANG ; Miao JIN ; Xiao-hui DUAN
Chinese Journal of Medical Genetics 2013;30(1):31-35
OBJECTIVETo investigate genetics and clinical characteristics of dentatorubral-pallidoluysian atrophy (DRPLA) in Chinese kindreds.
METHODSFragment analysis with laser-induced fluorescence in capillary electrophoresis was performed for the cytosine-adenine-guanine (CAG) repeats of DRPLA gene in 708 probands of autosomal dominant ataxia pedigrees and 119 sporadic ataxia cases.
RESULTSExpanded CAG repeats of DRPLA gene were detected in probands of three ataxia pedigrees, with the numbers of repeats being 16/58, 16/58 and 14/54, respectively. In addition to ataxia, patients with adult-onset disease also exhibited spasm and neck torsion.
CONCLUSIONOnly three cases of DRPLA have been identified among 827 cases, which suggested that DRPLA is a relatively rare subtype of SCA in Chinese population. Clinical variation among the patients suggested DRPLA has a wide spectrum of phenotype.
Adolescent ; Adult ; Aged ; Asian Continental Ancestry Group ; Brain ; pathology ; Child ; Child, Preschool ; China ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Mutation ; Myoclonic Epilepsies, Progressive ; diagnosis ; genetics ; Nerve Tissue Proteins ; genetics ; Pedigree ; Phenotype ; Trinucleotide Repeats ; Young Adult
8.Observation for the Impact of Nitroglycerin on Radial Artery Structure by Optical Coherence Tomography
Xi-Le BI ; Yan-Ming FAN ; Yan-Bo WANG ; Yu-Yang XIAO ; Xin-Shun GU ; Guo-Zhen HAO ; Yun-Fa JIANG ; Qing-Sheng WANG ; Xiang-Hua FU
Chinese Circulation Journal 2018;33(1):36-40
Objective: To observe the lumen structural changes of radial artery (RA) in patients with transradial coronary intervention and the impact of nitroglycerin on the structure by optical coherence tomography (OCT). Methods: A total of 20 patients with transradial coronary intervention were enrolled for OCT imaging to observe and compare the lumen structures of RA between the basic condition and nitroglycerin treated condition. Results: OCT imaging found that 15/20 patients had radial spasm and 1 had intimal tear. Compared to basic condition, with nitroglycerin treatment, the mean lumen diameter, lumen area and total vascular area were increased in the distal, middle and proximal portion of RA, all P<0.001; the intima-media thickness was decreased in the distal, middle and proximal portion of RA, all P<0.001; while the cross section area of tunica media, intimal thickness and extravascular membrane thickness were similar between the basic condition and nitroglycerin treated condition, all P>0.005. Conclusion: Vasodilatation drug may obviously enlarge RA lumen area and total vascular area in patients after transradial coronary intervention.
9.Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography.
Long Hao JIN ; Byung Hyune CHOI ; Young Jick KIM ; Hyun Ju OH ; Byoung Ju KIM ; Xiang Yun YIN ; Byoung Hyun MIN
Tissue Engineering and Regenerative Medicine 2018;15(3):311-319
It is very useful to evaluate the content and 3D distribution of extracellular matrix non-destructively in tissue engineering. This study evaluated the feasibility of using micro-computed tomography (µCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffolds in vitro. Engineered cartilages were incubated with Hexabrix 320 for 20 min and analyzed via µCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D µCT images of an engineered cartilage showed positive correlation with the histological image of Safranin-O staining. Quantitative data obtained with the 3D µCT images of 14 engineered cartilages showed strong correlation with sulfated GAGs contents obtained by biochemical analysis (R² = 0.883, p < 0.001). Repeated exposure of engineered cartilages to Hexabrix 320 and µCT scanning did not significantly affect cell viability, total DNA content, or the total content of sulfated GAGs. We conclude that µCT imaging using Hexabrix 320 provides high spatial resolution and sensitivity to assess the content and 3D distribution of sulfated GAGs in engineered cartilages. It is expected to be a valuable tool to evaluate the quality of engineered cartilage for commercial development in the future.
Cartilage*
;
Cell Survival
;
Chondrocytes
;
DNA
;
Extracellular Matrix
;
Glycosaminoglycans*
;
In Vitro Techniques
;
Ioxaglic Acid
;
Polyglycolic Acid
;
Tissue Engineering
10.Subjective sleepiness in heart failure patients with sleep-related breathing disorder.
Han-Qiao WANG ; Gang CHEN ; Jing LI ; Shu-Min HAO ; Xin-Shun GU ; Jiang-Na PANG ; Xiang-Hua FU
Chinese Medical Journal 2009;122(12):1375-1379
BACKGROUNDPrevious studies show that sleep-related breathing disorder (SRBD) is common in patients with heart failure (HF) and is associated with increased mortality. This study aimed to determine whether there was significant difference of subjective daytime sleepiness between HF patients with and without SRBD.
METHODSWe enrolled, prospectively, 195 consecutive HF patients with left ventricular ejection fractions (LVEF) < or = 45% and all subjects underwent polysomnography to measure the sleep structure between 2005 and 2008. Patients were then assigned to those with SRBD including obstructive and central sleep apnea (apnea-hypopnea index (AHI) > or = 5/hour of sleep) and those without SRBD (AHI < 5/hour) according to the sleep study. The subjective sleepiness was assessed with Epworth sleepiness scale (ESS).
RESULTSAmong 195 HF patients, the prevalence of obstructive sleep apnea (OSA) was 53% and of central sleep apnea (CSA) was 27%. There was no significant difference of ESS scores between patients without SRBD (NSA) and with SRBD (NSA vs OSA: 6.7 +/- 0.6 vs 7.6 +/- 0.4, P = 0.105 and NSA vs CSA: 6.7 +/- 0.6 vs 7.4 +/- 0.5, P = 0.235, respectively), indicating that SRBD patients had no more subjective daytime sleepiness. Compared with NSA, patients with SRBD had increased arousal index (ArI) (NSA vs OSA: 14.1 +/- 1.4 vs 26.3 +/- 1.5, P < 0.001 and NSA vs CSA: 14.1 +/- 1.4 vs 31.3 +/- 3.5, P < 0.001, respectively), more awake number after sleep onset (NSA vs OSA: 19.2 +/- 1.5 vs 26.2 +/- 1.4, P = 0.01 and NSA vs CSA: 19.2 +/- 1.5 vs 36.9 +/- 4.4, P < 0.001, respectively), and reduced proportion of slow-wave sleep (SWS) (NSA vs OSA: 13.8 +/- 1.7 vs 9.3 +/- 0.7, P = 0.024 and NSA vs CSA: 13.8 +/- 1.7 vs 8.9 +/- 0.9, P = 0.024, respectively).
CONCLUSIONSOSA and CSA remain common in patients with HF on optimal contemporary therapy. Patients with both HF and SRBD have no significant subjective daytime sleepiness compared with patients without SRBD, despite of significantly increased awake number, arousal and decreased proportion of deep sleep stages. It is not a credible way and means to exclude SRBD in patients with HF according to the absence of subjective daytime sleepiness.
Adult ; Aged ; Aged, 80 and over ; Female ; Heart Failure ; physiopathology ; Humans ; Male ; Middle Aged ; Polysomnography ; Sleep Apnea Syndromes ; epidemiology ; etiology