Objective To investigate the clinical value of the intrahepatic Glisson's sheath vascular disconnection approach for anatomical hepatectomy by three-dimensional (3D) laparoscope.Methods Twenty-two patients(11 liver cancer and 11 benign diseases)underwent hepatectomy in Zhujiang Hospital,Southern Medical University from November 2013 to March 2015 were involved.All patients were performed anatomical hepatectomy by 3D laparoscope with the intrahepatic Glisson's sheath vascular disconnection approach.The anatomical time of first hepatic portal,operation time,intraoperative bleeding,postoperation hospitalization and the complication incidences were observed.Results All the 22 cases were performed successfully with the intrahepatic Glisson's sheath vascular disconnection and the anatomic hepatectomy.The anatomical time of first hepatic portal,operation time,intraoperative bleeding,the postoperation hospitalization duration and complication incidence rate were (43 ± 14) minutes (22-74 minutes),(295 ± 89) minutes (105-480 minutes),(546 ± 390) ml(50-1 500 ml),(9.8 ±2.8) days(5-16 days) and 18.2％ (4/22)in 22 patients,respectively.There was no death.Conclusions With the help of 3D laparoscope,the anatomy of Glisson pedicle could perform precisely and the dissection of vessels would be easy and accuracy.The 3D laparoscope is feasible for performing the anatomical hepatectomy.

Objective To investigate the clinical value of the intrahepatic Glisson's sheath vascular disconnection approach for anatomical hepatectomy by three-dimensional (3D) laparoscope.Methods Twenty-two patients(11 liver cancer and 11 benign diseases)underwent hepatectomy in Zhujiang Hospital,Southern Medical University from November 2013 to March 2015 were involved.All patients were performed anatomical hepatectomy by 3D laparoscope with the intrahepatic Glisson's sheath vascular disconnection approach.The anatomical time of first hepatic portal,operation time,intraoperative bleeding,postoperation hospitalization and the complication incidences were observed.Results All the 22 cases were performed successfully with the intrahepatic Glisson's sheath vascular disconnection and the anatomic hepatectomy.The anatomical time of first hepatic portal,operation time,intraoperative bleeding,the postoperation hospitalization duration and complication incidence rate were (43 ± 14) minutes (22-74 minutes),(295 ± 89) minutes (105-480 minutes),(546 ± 390) ml(50-1 500 ml),(9.8 ±2.8) days(5-16 days) and 18.2％ (4/22)in 22 patients,respectively.There was no death.Conclusions With the help of 3D laparoscope,the anatomy of Glisson pedicle could perform precisely and the dissection of vessels would be easy and accuracy.The 3D laparoscope is feasible for performing the anatomical hepatectomy.

The present study investigated the relationship between DNA-dependent protein kinase (DNA-PK) and radiosensitivity of nasopharyngeal carcinoma (NPC) cell lines. The dose-survival relationship for NPC cell lines, CNE1 and CNE2, was analyzed using clonogenic formation assay, the activity of DNA-PK of the two cell lines was measured using the Signa TECT DNA-PK assay kit, and the localization and expression of Kus (a heterodimer) and DNA-PKcs protein in CNE1 and CNE2 before irradiation and 15 min, 1 h, 6 h, 12 h, 24 h after 4 Gy irradiation were analyzed by immunofluorescence, laser scanning confocal microscope (LSCM) and Western blot. The results showed that the surviving fraction of CNE1 was higher than that of CNE2 at each dose. The DNA-PK activity of CNE1 was also significantly higher than that of CNE2 before and after irradiation (P<0.05), while the expression of total Ku70/Ku80 in CNE1 and CNE2 had no significant difference. Increasing translocation of Ku70 and Ku80 from the cytoplasm to the nuclei in the two cell lines was observed with increase of irradiation time as detected by Western blot, and the immunofluorescence of the DNA-PK complex subunits showed greater nuclear translocation in CNE1 than CNE2 after irradiation. The results suggest that the relatively higher radio-resistance of CNE1 correlates with the higher activity of DNA-PK as compared to that of more radiosensitive CNE2 (or lower radio-resistance) before and after irradiation. Thus, DNA-PK activity may be a useful predictor of radiosensitivity of NPC.
Carcinoma ; Cell Line, Tumor ; enzymology ; radiation effects ; DNA-Activated Protein Kinase ; metabolism ; Humans ; Nasopharyngeal Neoplasms ; enzymology ; Radiation Tolerance

BACKGROUNDThe prevalence of malocclusion in modern population is higher than that in the excavated samples from the ancient times. Presently, the prevalence of juvenile malocclusion in the early stage of permanent teeth is as high as 72.92% in China. This study aimed to observe and evaluate the prevalence and severity of malocclusions in a sample of Xia Dynasty in China, and to compare these findings with the modern Chinese population.

METHODSThe material consisted of 38 male and 18 female protohistoric skulls of Xia Dynasty 4000 years ago. Of 86 dental arches, 29 cases had the jaw relationships. Tooth crowding, diastema, individual tooth malposition and malocclusion were studied.

RESULTSOf the samples, 23.3% showed tooth alignment problems including crowding (8.1%), diastema (9.3%), and individual tooth malposition (5.8%). The prevalence of malocclusion was 27.6%, mainly presented as Angle Class I.

CONCLUSIONSIt is indicated that over thousands of years from Neolithic Age (6000 - 7000 years ago) to Xia Dynasty (4000 years ago), the prevalence of malocclusion did not change significantly. The prevalence of malocclusion of Xia Dynasty samples was much lower than that of modern population.

China ; epidemiology ; Diastema ; Female ; History, Ancient ; Humans ; Male ; Malocclusion ; epidemiology ; history

OBJECTIVETo explore the effect of the improved plastic and reconstruction of the anus in situ.

METHODSImproved plastic and reconstruction of anus in situ was performed in 38 cases of low rectal cancers operated while Miles radical operation. Improvement includes: (1) The internal sphincter was rebuilt with 4 layers of muscle layer of the endmost of colon. (2) The last of gracilis was divided into 2 parts to reconstruct the superficial part and deep part of external sphincter muscle. (3) The rectum cape improvement is to firmly stitch the levator ani outside the external sphincter muscle in front of the colon. (4) The rectum valve is improved into three artificial rectum valves.

RESULTSThe form and function and their long term survival rate were good, the rate of superior anus function was 94.73%.

CONCLUSIONIt mains the results of improved plastic and reconstruction of anus in situ is near that of normal persons.

Adult ; Aged ; Anal Canal ; surgery ; Female ; Humans ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Rectum ; surgery

Objective：The aim of this study was to evaluate radiation induced temporomandibular joint damage in the patients with nasopharyngeal carcinoma, and its correlative factors were analyzed. Methods： From November 7, 1966 to July 2, 1999, 352 patients with nasopharyngeal carcinoma received radical conventional radiotherapy were eligible for this analysis. The total dose of the temporomandibular joint were 51.90－ 78.89 gray and the overall treatment time were 35－ 141 days. The endpoint was the distance between two dens incisivus medialis (DDIM). The relationship between total doses and temporomandibular joint lesion was illustrated with curve estimation. Multivariate analysis with Logistic Regress was performed to evaluate the significance of prognostic variables on temporomandibular joint lesion. Results： The patient of DDIM60.00－ 70.00 gray, and >70.00－ 78.89 gray were 46.4％ , 53.5％ , and 62.3％ , respectively (P=0.050); and in the patients who insisted in opening mouths practice or not were 51.6％ and 61.7％ respectively (P=0.028); and in patients at age of 18－ 42 and 43－ 71 were 54.1％ and 62.7％ respectively(P=0.040). They were all significant prognostic factors for radiation induced temporomandibular joint lesion. Conclusion： Radiation induced temporomandibular joint lesion in radiation treated NPC patients was serious. The total dose of temporomandibular joint, opening mouths practice and age are three significant prognostic factors.

OBJECTIVETo investigate the changes in brain injury after the induction chemotherapy in children with acute lymphoblastic leukemia (ALL) by cranial MRI.

METHODSThe clinical data and cranial MRI results of 62 children with ALL who were hospitalized from March 2014 to June 2015 were analyzed retrospectively.

RESULTSBefore chemotherapy, MRI showed bone marrow infiltration of the skull in 33 patients (53%); the children with WBC<20×10(9)/Lhad a significantly lower incidence rate of bone marrow infiltration of the skull than those with WBC≥20×10(9)/L (16 patients/42% vs 17 patients/71%; P<0.05), and the high-risk group had a significantly higher incidence rate of bone marrow infiltration of the skull than the non-high-risk group (71% vs 44%; P<0.05). Before chemotherapy, there were 4 cases (7%) of brain atrophy, and 2 cases (3%) of abnormal signals in the sensory conduction bundle. MRI reexamination in 28 patients after 3 months of chemotherapy showed 3 new cases (11%) of brain atrophy and 1 aggravated case of brain atrophy.

CONCLUSIONSThe children with ALL have bone marrow infiltration of the skull, brain atrophy, and abnormal signals in the sensory conduction bundle before chemotherapy, especially bone marrow infiltration of the skull, and some changes in brain injury disappear after treatment.

Adolescent ; Bone Marrow ; pathology ; Brain ; drug effects ; pathology ; Child ; Child, Preschool ; Female ; Humans ; Induction Chemotherapy ; adverse effects ; Infant ; Magnetic Resonance Imaging ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; pathology ; Retrospective Studies ; Skull ; pathology

OBJECTIVETo clone the recombinant human islet neogenesis-associated protein (rhINGAP) gene for its secretory expression in Pichia pastoris.

METHODSINGAP gene was amplified with PCR and inserted between Xho I and EcoR I downstream sites of the alpha factor of the recombinant plasmid alpha/pUC18. The fusion gene of alpha factor and INGAP was subsequently inserted between BamH I and EcoR I sites of the plasmid pPIC9K of P. pastoris. After confirmation with restriction enzyme digestion and sequencing, the positive recombinant plasmid that integrated INGAP gene was linearized with Sal I digestion and transformed into the yeast host strain GS115 through electroporation. The yeast transformants that harbored the INGAP gene with high copies were selected with the auxotroph medium and G418, followed then by PCR verification of the positive transformants, from which the expression of recombinant human INGAP was induced with methanol as the only carbone source. The antigenic activity of the desired protein was then detected using Western blotting and enzyme-linked immunosorbent assay (ELISA).

RESULTS AND CONCLUSIONThe recombinant expression plasmid INGAP/pPIC9K was successfully constructed, and 3 positive transformants were obtained. The expressed protein showed good antigenic activity as confirmed by Western blotting and ELISA.

Antigens, Neoplasm ; genetics ; metabolism ; Biomarkers, Tumor ; genetics ; metabolism ; Blotting, Western ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; Humans ; Islets of Langerhans ; metabolism ; Lectins, C-Type ; genetics ; metabolism ; Pancreatitis-Associated Proteins ; Pichia ; genetics ; metabolism ; Recombinant Proteins ; metabolism

OBJECTIVETo establish a favorable formula of estimation adult standard liver volume (SLV) based on the data of living donors for liver transplantation.

METHODSFrom March 2005 to December 2007, 90 Adult-to-Adult living donor liver transplantation were performed. The donors' anthropometric data of gender, year, body weight and body height was collected prospectively. The total liver volume (TLV) of 90 living donors was measured by computed tomography. The correlation between TLV and several factors including body weight index (BMI) and body surface area (BSA) were analyzed. Then multiple stepwise linear regression analysis was performed and a new equation predicting SLV to improve approximate TLV was determined. TLV was then compared with the estimation standard liver volume (ESLV) calculated using our formula and published formulas in literature previously.

RESULTSAll the subjects had a mean body weight of (62.4 +/- 8.7) kg. The mean total liver volume was (1319.1 +/- 167.0) ml. There were positively correlated between TLV and body weight (BW), which could be expressed in the equation: TLV (ml) = 12.5 x BW (kg) + 536.4 (r(2) = 0.43, P < 0.01). Compared TLV with ELSV, which were calculated using previously published formulas based on the anthropometric data of the 90 subjects, the difference was be statistically significant.

CONCLUSIONA new simple formula is established that might be a more suitable to calculate TLV in Chinese adults.

Adult ; Female ; Humans ; Linear Models ; Liver ; anatomy & histology ; diagnostic imaging ; Liver Transplantation ; Living Donors ; Male ; Middle Aged ; Organ Size ; Tomography, X-Ray Computed

OBJECTIVETo evaluate the advantage and disadvantage of laser-assisted liposuction compared with conventional liposuction.

METHODSTen swines were devided into three groups, the laser liposuction group, conventional liposuction group and control group. We compared the two surgical groups with the following aspects: ecchymosi, edema, lipocrit study, hemoglobin studies and blood biochemical changes, etc.

RESULTSThere is a benefit of laser-assisted liposuction in the following aspects such as ecchymosi, edema, lipocrit and postoperative complications. The two aspects, that is, ecchymosi and lipocrit study was statistically significant. Hemoglobin change was not statistically significant between the two groups. Laser-assisted liposuction did not lead to dysfunction of organs such as liver and kidney.

CONCLUSIONSLaser-assisted liposuction can significantly decrease the blood lose, ecchymosis and edema compared with conventional liposuction, and it did not give rise to dysfunction of organs.

Adipose Tissue ; surgery ; Animals ; Female ; Lasers ; Lipectomy ; instrumentation ; methods ; Male ; Models, Animal ; Swine ; Treatment Outcome