Calcitonin ; blood ; Fluorine ; adverse effects ; Humans ; Occupational Exposure ; adverse effects ; Osteocalcin ; blood

Accreditation ; Laboratories ; standards ; Pathology ; standards

Objective To observe the efficacy of self-made Arnebiae oil by our hospital on phaseⅠandⅡpressure ulcers. Methods A total of 324 pressure wounds of 283 cases collected from March 2014 to August 2015 were randomly divided into the observation group (145 cases) and the control group (138 cases). The control group received Kangfuxin solution in conjunction with conventional pressure ulcer care treatment, the observation group were treated with Arnebiae oil and conventional pressure ulcer care treatment. The therapeutic effect and healing time of both groups were compared. Results Arnebiae oil was effective in 96.97% (160/165) of the pressure ulcer patients and cured 84.85% (140/165) patients in the observation group. Nearly 84.91%(135/159) patients were effective and 50.94%(81/159) patients were cured in the control group. There was statistical significance (χ2=42.93, 14.46, P<0.01). The healing time was (8.78±4.27) d in the observation group and (10.46±3.99) d in the control group with significance(t=2.88, P< 0.05). The healing rates of Arnebiae oil sacrococcygeal region, hips, legs and other parts were 87.96%(95/108), 9/10, 12/16, 9/9, significantly higher than those of the control group, 46.43%(39/84), 6/12, 3/9, 9/15, the difference had statistical significance (χ2=4.02-44.35, P<0.05). Conclusion Arnebiae oil com-bined with the overall care can improve the rate of wound healing in aged pressure ulcer, which is suitable for clinical application.

Adenocarcinoma, Follicular ; genetics ; metabolism ; pathology ; Carcinoma, Papillary ; genetics ; metabolism ; pathology ; Carcinoma, Renal Cell ; genetics ; metabolism ; pathology ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Kidney Diseases, Cystic ; genetics ; metabolism ; pathology ; Kidney Neoplasms ; genetics ; metabolism ; pathology ; Thyroid Neoplasms ; genetics ; metabolism ; pathology ; Translocation, Genetic

Objective To analyse the sensitivity ,specificity and coincidence rate of genechip method in the detection of resistance to antibacterial agents in Mycobacterium tuberculosis(MTB) ,in order to provide a convenient ,accurate and rapid method for detec‐ting antibacterial resistance in MTB .Methods The DNA sequencing was taken as gold standard ,and antibacterial resistance of the strains of MTB isolated from sputum specimens of 250 cases of patients with tuberculosis from August to December 2014 were de‐tected by using the genechip method and proportion method for susceptibility testing at the same time .Efficacies of the two methods in detecting MTB resistance to rifarnpin and isoniazid were compared .Results The MTB resistance rate to rifarnpin detected by u‐sing the genechip method and proportion method for susceptibility testing was 3 .0% and 3 .5% respectively ;that to isoniazid was 6 .7% and 8 .2% respectively .For detecting M TB resistance to rifarnpin and isoniazid ,the DNA sequencing was taken as gold standard ,the sensitivity ,specificity and coincidence rate of genechip method was higher than those of proportion method for suscep‐tibility testing ,and the test time of genechip method was shorter than that of proportion method for susceptibility testing ,there were statistically significant differences(P<0 .05) .Conclusion Using the genechip method to detecting MTB resistance to rifampin and isoniazid has high sensitivity ,specificity and coincidence rate ,which could replace the proportion method for susceptibility tes‐ting and become an effective method .

Bone Neoplasms ; Humans ; Ilium ; Paraganglioma ; Sacrum

Animals ; Bone Matrix ; pathology ; Bone and Bones ; pathology ; Collagen Type I ; metabolism ; Collagen Type III ; metabolism ; Disease Models, Animal ; Humans ; Muscle, Skeletal ; metabolism ; pathology ; Osteoblasts ; pathology ; ultrastructure ; Osteoclasts ; pathology ; Osteocytes ; pathology ; Osteogenesis Imperfecta ; classification ; metabolism ; pathology ; Skin ; pathology

OBJECTIVETo evaluate the microleakage of fiber post and core systems after high-speed handpiece preparation at different time points.

METHODSThe crowns of forty-five extracted human premolar were removed and the roots were endodontically treated. The samples were devided into five groups. Root canal preparation was performed on each premolar followed by fiber post cementation and core build up. Tooth preparation was applied at 5 min in group 1, at 15 min in group 2 and at 30 min in group 3 after post cementation. Five teeth with only 5 mm apical sealing were served as a positive control group, and ten with fiber post and core build-up but no coronal preparation were taken as a negative control group. Microleakage was evaluated using a fluid filtration system. The bonding interface was observed by scanning electronic microscope (SEM).

RESULTSThe microleakage was significantly increased after coronal preparation with high-speed handpiece. The negative control group has less leakage [(1.50 × 10(-6) ± 0.37 × 10(-6)) µl×min(-1)×Pa(-1)] than the groups with coronal preparation (P < 0.05); Group 1 leaked significantly more [(6.02 × 10(-5) ± 1.02 × 10(-5)) µl×min(-1)×Pa(-1)] than group 2 [(1.50 × 10(-5) ± 0.26 × 10(-5)) µl·min(-1)×Pa(-1)] and group 3 [(1.50 × 10(-5) ± 0.39 × 10(-5)) µl×min(-1)×Pa(-1)] did (P < 0.05). Corresponding to microleakage, the micro gaps between the resin cement and dentine in group 1 were wider than those in the other groups. The coronal section was wider than the apical part.

CONCLUSIONSHigh-speed handpiece had negative effects on microleakage of fiber post and core systems. Coronal preparation should be performed 15 min or more after post cementation.

Cementation ; Dental Bonding ; Dental Leakage ; Dentin-Bonding Agents ; Humans ; Microscopy, Electron, Scanning ; Post and Core Technique ; instrumentation ; Resin Cements ; Root Canal Preparation ; Time Factors

Objective To compare the effect of airway clearance system (VestTM system) and traditional person knocking method on expectoration and pulmonary infection prevention in elderly patients undergone abdominal operation. Methods 60 cases of elderly patients undergone abdominal operation were randomly divided into observation group (n = 30) and control group (n = 30). The control group received person knocking method to promote expectoration while the observation group was treated with VestTM system.Clinical effect was compared between the groups. Results Sputum volume on day 1 of the observation group [(25.32 ± 2.12) ml/d] was higher than that of the control group [(14.20 ± 1.58) ml/d], and the difference was statistically significant (t = 23.04, P ＜ 0.05). There were also significant differences of sputum volume on day 2, day 3 and day4 (P＜0.05). 6 cases of cut pain, (4.2±0.2) scores of VAS and3 cases of difficulty in position cooperation were reported in the observation group, while the control group reported 21 cases,(7.2±0.3) scores and 16 cases, respectively, the differences were statistically significant (x2 = 13.20,11.09, respectively, t = 45. 57, P ＜ 0.05). There was no significant difference regarding hemodynamics and drainage volume (P ＞ 0.05). Conclusions Compared with traditional person knocking method, the VestTM system has satisfying effect of expectoration and popularity with patients, and can effectively prevent pulmonary infection in elderly patients undergone abdominal operation.