Objective To investigate the effect of finger exercises on recovery of hand function and daily life ability in postoperative patients with bone trauma. Methods Ninety-eight phalanx trauma patients were equally divided into the treatment and control groups, those with odd admission number in the treatment group and those with even numbers in the control group: the control group was given postoperative routine care and the treatment group took finger exercises besides the routine nursing. The two groups were compared in terms of the treatment effect and the ability of daily life . Result The effective rate of the treatment group was higher than that of the control group and the ability of daily life was significantly better than that of the control group (all P < 0.05). Conclusion The postoperative finger exercises of the patients with bone trauma can increase the recovery of hand function , which is beneficial for the improvement their daily life ability.

Gastrectomy ; methods ; Humans ; Pylorus ; surgery ; Stomach Neoplasms ; surgery

OBJECTIVETo verify the clinical efficacy on post-stroke dysphagia treated with acupuncture at Lianquan (CV 23).

METHODSOne hundred and eighty patients were randomized into an acupuncture A group, an acupuncture B group and a rehabilitation group, 60 cases in each one. On the basis of the conventional medication, in the acupuncture A group, acupuncture was applied at Lianquan (CV 23); in the acupuncture B group, acupuncture was applied at Hegu (LI 4) and Neiguan (PC 6) and in the rehabilitation group, the swallowing rehabilitation training was adopted. The treatment was given once a day, 5 times a week, and the 4 weeks of treatment was required in all of the groups. The national institute of health stroke scale (NIHSS) and TV X-ray fluoroscope swallowing scale (VFSS) were used to evaluate neurologic deficit and swallowing function before and after treatment in the patients of each group. The morbidity of pneumonia and clinical efficacy were compared among the groups.

RESULTSThe scores of NIHSS and VFSS were improved apparently after treatment in the patients of the three groups (all P < 0.05) and the results in the acupuncture A group were superior to those in the other two groups (all P < 0.05). The morbidity of pneumonia in the acupuncture A group was lower than that in the acupuncture B group and the rehabilitation group [3.3% (2/60) vs 6.7% (4/60), 8.3% (5/60), both P < 0.05]. The effective rate in the acupuncture A group was better than that in either of the other two groups [95.0% (57/60) vs 81.7% (49/ 60), 75.0% (45/60), both P < 0.05].

CONCLUSIONOn the basis of the conventional medication, acupuncture at Lianquan (CV 23) effectively improves the swallowing function, relieves neurological deficit and reduces the morbidity of pneumonia in the patients of post-stroke dysphagia.

Acupuncture Therapy ; Aged ; Deglutition ; Deglutition Disorders ; etiology ; physiopathology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Quality of Life ; Stroke ; complications ; Treatment Outcome

Animals ; Antioxidants ; physiology ; Dehydroepiandrosterone ; physiology ; Lipid Metabolism ; physiology ; Male ; Oxidative Stress ; drug effects ; Rats ; Rats, Sprague-Dawley

Acute Disease ; Angioplasty, Balloon, Coronary ; adverse effects ; Compartment Syndromes ; etiology ; Female ; Humans ; Middle Aged

Objective To explore the characteristics of lymph node metastasis in gastric stump cancer and the efficacy of surgical treatment. Methods The clinical data of 42 patients with gastric stump cancer (test group) and 56 patients with primary cancer in the upper stomach (control group) who were admitted to The First Affiliated Hospital of Dalian Medical University from January 1994 to January 2008 were retrospectively analyzed. All data were analyzed by t test and chi-square test. The survival of the patients and lymph node metastasis rate were analyzed using the Kaplan-Meier method and Log-rank test, respectively. Results The metastasis rates of lymph nodes 1-3 of the test group and control group were similar (43%-61%), with no significant difference between the 2 groups (χ2 = 0.752, 0. 833, 0. 678, P > 0. 05). The metastasis rates of lymph nodes 7-9 of the test group and control group were 22% , 18% , 25% and 46% , 25% , 30% , respectively, and this was signifi-cantly different between the 2 groups (χ2 = 2. 168, 3. 263, 5. 761, P < 0. 05). The metastasis rates of lymph nodes 10-14 were 47% , 36% , 31% , 20% and 25% in the test group, which were significantly higher than 33% , 34% , 19% , 6% and zero in the control group, respectively (χ2 =3.225, 1.883, 3.945, 4.137, 6.823, P <0.05). The metastasis rate of lymph node no. 16 was zero in the test group and 23% in the control group. The metastasis rate of jejunal mesenteric lymph nodes was 27% in the test group and zero in the control group. The accumulative 5-year survival rate of patients was 38% in the test group and 48% in the control group, and this was significantly different (χ2 =4. 165, P<0.05). The overall 5-year survival rate of patients with radical resection was not significantly different between degree A (54%) and B (57%) (χ2 = 0. 622, P > 0. 05). Conclusions Gastric stump cancer has a unique pattern in lymph node metastasis. Surgical resection is effective in improving the prognosis of patients with gastric stump cancer.

Objective Glutarnic acid, an important excitatory neurotransmitter, plays an important role in morphine dependence and tolerance. Astrocyte (AST) takes up giutamic acid which is transformed into glutamine, the precursor of GABA, by means of intracellular glutaminase. The aim of thin study was to investigate the effect of ketamine on glutamate release in cultured spinal ASTs chronically treated with morphine. Methods ASTs were isolated from 1-3 day old SD rats and divided into 8 groups : control group and group A, B1, B2, B3 , C1, C2, C3. The isolated ASTs were cultured and incubated for 48h in the presence (group A, B1-3, C1-3) and absence (control group) of 10?mol?L-1 morphine.Then the ASTs were transferred to liquid culture medium Neurobasal / B27 containing no serum. No drug was added in group A. Morphine 0.1 ,1 or 10?mol?L-1 was added in group B1-3 and ketamine 0.4, 4 or 40?mol?L-1 in group C1-3. After being incubated for 15 min, naloxone 10?mol?L-1 was added in group B1-3 and C1-3. After another 30 min incubation the gluamate concentration in supernatant was measured using HPLC. Results There was no significant difference in glutamate concentration between control group and group A ( P

Objective Recent studies have shown that activation of spinal astrocytes (ASTs) may be involved in the development of morphine tolerance. The purpose of this study was to investigate the effect of ketamine (K) on spinal ASTs in mice tolerant to morphine (M) .Methods Thirty Kun-Ming mice of both sexes weighing 18-22 g were randomly divided into 5 groups of six animals each : (A) control group received only subcutaneous (s.c.) and intraperitoneal (i.p.) normal saline (NS); (B) chronic M-tolerance group received M 10 mg?kg-1 s.c. followed after 30 min by NS 10 ml?kg-1 i.p. twice a day (at 8:00 and 17:00) for 9 days;(C), (D), (E) K group received M 10 mg?kg-1 s.c. followed after 30 min by K 5 mg? kg-1(C), 10 mg?kg-1 (D) or 20 mg?kg-1 (E) i.p. twice a day for 9 days. Pain threshold was estimated by measuring paw withdrawal response to Von Frey filament stimulation every other day (1st, 3rd, 5th, 7th, 9th) In after second administration of drugs. The percentage of maximal possible effect (MPE% ) was calculated : MPE% = [ (test group PWTV - control group PWTV) / (15 - control group PWTV)] ? 100% (PWTV = paw withdrawal threshold value). On the 9th day after pain threshold was measured the animals were sacrificed and lumbosacral segment of spinal cord was removed. The changes in spinal ASTs were detected by immunohistochemistry. The average areas of GFAP immuno-reactive cells in the dorsal horn were measured to show the degree of spinal AST activation. Results 1. MPE% was 0 at all time points in group A. In group B MPE% was 42.8% on the 1st and 3rd day and gradually decreasing on the 5th and 7th day and became 0 on the 9th day signifying full development of morphine tolerance. In group C the change in MPE% was almost the same as in group B. In group D and E MPE % tended to decrease but was still above 30% at all time points signifying that ketamine 10 and 20 mg?kg-1 could partly antagonize the development of morphine tolerance. 2. In group B the staining of GFAP immuno-reactive cells was heavier and the average areas were significantly larger than in group A (P

OBJECTIVE:To establish HPLC fingerprint sectrum of Rhaponticum uniflorum. METHODS:HPLC was performed on the column of Hypersil-ODS with mobile phase of 0.3% phosphoric acid-acetonitrile(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 30 ℃ and volume injection was 10 μl. The luteolin was refer-ence,17 batches of R. uniflorum from different production places was analyzed and similarity evaluation system for chromatograph-ic fingerprint of TCM (2004 A edition)was adopted for the similarity analysis. RESULTS:There were totally 11 common peaks with similarity degree≥0.900 of 17 batches. According to the verification,the fingerprint spectrum and reference fingerprint spec-trum of R. uniflorum had good consistency. CONCLUSIONS:The established method is specific and stable,and can provide refer-ence for the identification and quality control of R. uniflorum.

Objective To investigate the effects of 25‐hydroxy vitamin D [25(OH)D] status on the pregnancy outcome in the second and third trimester pregnant women living .Methods A total of 143 pregnant women (12 -42 weeks gestational age) were participated in the study .Their serum 25(OH)D concentrations were detected by liquid chromatography‐mass spectrometry method .Finally ,vitamin D status was assessed according to sufficiency group(n= 32) ,inadequacy group(n= 49) ,insufficiency group(n=51) and deficiency group(n=11) .Retrospective analysis were adopted to follow up the pregnancy outcome of pregnant women .Results (1)The average serum 25(OH)D level was(22 .49 ± 9 .51)ng/mL in the second and third trimester of pregnancy . (2)Abortion rate were 3 .1% (1/32) in sufficient group ,2 .0% (1/49)in inadequacy group ,11 .8% (6/51) in insufficiency group , 36 .4% (4/11) in deficiency group ,fetal distress incidence were 3 .1% (1/32) ,8 .2% (4/49) ,11 .8% (6/51) ,36 .4% (4/11) in each group .Abortion ,fetal distress rate were higher in deficiency group compared with the other three groups(P<0 .05) .(3)Bacterial vaginosis incidence were 0(0/32) ,4 .1% (2/49) ,13 .7% (7/51) ,27 .3% (3/11)in each group ,deficiency group and insufficiency group were higher(P<0 .05) .(4)Otherwise ,the gestation period were (39 .48 ± 1 .44) weeks in sufficient group ,(39 .00 ± 1 .70) weeks in inadequacy group ,(38 .09 ± 2 .01) weeks in insufficiency group ,(37 .29 ± 0 .49) weeks in deficiency group ,pregnancy cycle was significantly shorter when 25(OH)D<20 .00 ng/mL (P<0 .05) .Conclusion Vitamin D deficiency in pregnant women could increase the risk of abortion ,fetal distress ,bacterial vaginosis ,and significantly shorten the gestation period .